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Information on EC 3.6.1.43 - dolichyldiphosphatase
for references in articles please use BRENDA:EC3.6.1.43
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EC Tree 3 Hydrolases
3.6 Acting on acid anhydrides
3.6.1 In phosphorus-containing anhydrides
3.6.1.43 dolichyldiphosphatase
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
showSynonyms
acid pyrophosphatase, dol-p-p phosphatase, dolichyl-diphosphate phosphatase, dppase, dolichyl pyrophosphatase, dolichyl pyrophosphate phosphatase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CAX4
Dol-P-P phosphatase
dol-P-P phosphohydrolase
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-
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Dol-PP phosphatase
dolichol diphosphatase
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-
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dolichyl diphosphate phosphohydrolase
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-
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dolichyl pyrophosphatase
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-
-
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dolichyl pyrophosphate phosphatase
dolichyl-diphosphate phosphatase
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-
-
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dolichyl pyrophosphate phosphatase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
dolichyl diphosphate + H2O = dolichyl phosphate + phosphate
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of diphosphate bonds
hydrolysis of diphosphate bonds
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-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
dolichyl-diphosphate phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
73361-26-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
dolichyl phosphate + H2O
dolichol + phosphate
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Substrates: -
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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Substrates: involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
Substrates: -
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: Dol-P produced by DPPase contributes significantly to the pool of Dol-P utilized for lipid intermediate biosynthesis on the cytoplasmic leaflet, dolichyl phosphate metabolism, overview
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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Substrates: calf brain microsomes, sealed vesicles, are used as substrates
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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Substrates: -
Products: -
Products: -
?
additional information
?
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Substrates: enzyme can not hydrolyse dolichyl monophospate
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme can not hydrolyse dolichyl monophospate
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: Dol-P produced by DPPase contributes significantly to the pool of Dol-P utilized for lipid intermediate biosynthesis on the cytoplasmic leaflet, dolichyl phosphate metabolism, overview
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
-
Substrates: -
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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bacitracin fails to inhibit the acid PPase
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bacitracin
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complete inhibition; no inhibition when preincubating with EDTA
divalent cations
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strong or complete inhibition at concentrations of 5 mM for all tested ions
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phosphate
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strong inhibition, 5 mM causes more than 60% loss of activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Influenza, Human
Characterization and comparison of the full 3' and 5' untranslated genomic regions of diverse isolates of infectious salmon anaemia virus by using a rapid and universal method.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.048
dolichyl phosphate
-
pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
0.04
dolichyl diphosphate
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pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.005
pH 7, 30°C, Dol-P-P phosphatase activity using microsomal fractions from Sf-9 cells expressing the enzyme
0.016
pH 7, 30°C, Dol-P-P phosphatase activity using microsomal fractions from COS cells expressing the enzyme
additional information
-
increases significantly during day 12-25 of embryonic development
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
absent in peroxisomes and inner mitochondrial membranes
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brenda
-
transmembrane protein with a luminally oriented active site
brenda
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plasma, outer mitochondrial membranes
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
physiological function
malfunction
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in enzyme-deficient cells showing endoplasmic reticulum stress, membrane and growth defect, the N-glycosylation of the vacuolar carboxypeptidase is severely affected, and expression of the endoplasmic reticulum chaperone Kar2p is elevated, which results in unfolded protein response activation as an adaptive response
malfunction
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in enzyme-deficient cells showing endoplasmic reticulum stress, membrane and growth defect, the N-glycosylation of the vacuolar carboxypeptidase is severely affected, and expression of the endoplasmic reticulum chaperone Kar2p is elevated, which results in unfolded protein response activation as an adaptive response
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metabolism
-
olicholpyrophosphatase participates in glycoprotein biosynthesis
physiological function
-
the enzyme regulates membrane biogenesis by coordinating lipid homeostasis with protein quality control
physiological function
-
the enzyme regulates membrane biogenesis by coordinating lipid homeostasis with protein quality control
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Show AA Sequence and Transmembrane Helices (2657 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C solubilized enzyme can be stored for 6 months without loss of activity
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Saccharomyces cerevisiae, COS cells and Sf-9 insect cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
lipids from the Sulfolobus membranes enhance the activity of the acid PPase to about 3fold
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wedgwood, J.F.; Strominger, J.L.
Enzymatic activities in cultured human lymphocytes that dephosphorylate dolichyl pyrophosphate and dolichyl phosphate
J. Biol. Chem.
255
1120-1123
1980
Homo sapiens
brenda
Belocopitow, E.; Boscoboinik, D.
Dolichyl-phosphate phosphatase and dolichyl-diphosphate phosphatase in rat-liver microsomes
Eur. J. Biochem.
125
167-173
1982
Rattus norvegicus
brenda
Bhat, N.R.; Frank, D.W.; Wolf, M.J.; Waechter, C.J.
Developmental changes in enzymes involved in dolichyl phosphate metabolism in cultured embryonic rat brain cells
J. Neurochem.
56
339-344
1991
Rattus norvegicus
brenda
Applekvist, E.L.; Chojnacki, T.; Dallner, G.
Dolichol (C55) mono-and pyrophosphatases of the rat liver
Biosci. Rep.
1
619-626
1981
Rattus norvegicus
-
brenda
Boscoboinik, D.O.; Belocopitow, E.
Assay of dolichol monophosphate and dolichol diphosphate phosphatases: thin-layer chromatography in a nonequilibrated system
Anal. Biochem.
114
42-45
1981
Rattus norvegicus
brenda
Scher, M.G.; Waechter, C.J.
Brain dolichyl pyrophosphate phosphatase. Solubilization, characterization, and differentiation from dolichyl monophosphate phosphatase activity
J. Biol. Chem.
259
14580-14585
1984
Bos taurus
brenda
Scher, M.G.; Waechter, C.J.
Dolichyl pyrophosphate phosphatase in brain
Methods Enzymol.
111
547-553
1985
Bos taurus
brenda
Shabalin, Y.A.; Hamut, A.; Bukhtiyarov, Y.E.; Kulaev, I.S.
Intracellular distribution and solubilization of yeast dolichylpyrophoshatase
Biochemistry (Moscow)
56
2032-2041
1991
Saccharomyces cerevisiae
-
brenda
Fernandez, F.; Rush, J.S.; Toke, D.A.; Han, G.S.; Quinn, J.E.; Carman, G.M.; Choi, J.Y.; Voelker, D.R.; Aebi, M.; Waechter, C.J.
The CWH8 gene encodes a dolichyl pyrophosphate phosphatase with a luminally oriented active site in the endoplasmic reticulum of Saccharomyces cerevisiae
J. Biol. Chem.
276
41455-41464
2001
Saccharomyces cerevisiae
brenda
Rush, J.S.; Cho, S.K.; Jiang, S.; Hofmann, S.L.; Waechter, C.J.
Identification and characterization of a cDNA encoding a dolichyl pyrophosphate phosphatase located in the endoplasmic reticulum of mammalian cells
J. Biol. Chem.
277
45226-45234
2002
Mus musculus (Q9JMF7), Mus musculus
brenda
Rush, J.S.; Gao, N.; Lehrman, M.A.; Waechter, C.J.
Recycling of dolichyl monophosphate to the cytoplasmic leaflet of the endoplasmic reticulum after the cleavage of dolichyl pyrophosphate on the lumenal monolayer
J. Biol. Chem.
283
4087-4093
2008
Saccharomyces cerevisiae
brenda
Manabe, F.; Itoh, Y.H.; Shoun, H.; Wakagi, T.
Membrane-bound acid pyrophosphatase from Sulfolobus tokodaii, a thermoacidophilic archaeon: heterologous expression of the gene and characterization of the product
Extremophiles
13
859-865
2009
Sulfurisphaera tokodaii
brenda
James, A.W.; Gowsalya, R.; Nachiappan, V.
Dolichyl pyrophosphate phosphatase-mediated N-glycosylation defect dysregulates lipid homeostasis in Saccharomyces cerevisiae
Biochim. Biophys. Acta
1861
1705-1718
2016
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
brenda
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