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Information on EC 3.5.4.9 - methenyltetrahydrofolate cyclohydrolase and Organism(s) Pseudomonas aeruginosa

for references in articles please use BRENDA:EC3.5.4.9
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IUBMB Comments
In eukaryotes, the enzyme occurs as a trifunctional enzyme that also has methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) and formate---tetrahydrofolate ligase (EC 6.3.4.3) activity. In some prokaryotes, it occurs as a bifunctional enzyme that also has dehydrogenase (EC 1.5.1.5) activity or formimidoyltetrahydrofolate cyclodeaminase (EC 4.3.1.4) activity.
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Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas aeruginosa
Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
mthfd2, cyclohydrolase, methenyltetrahydrofolate cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase, mthfd2l, 5,10-methenyl-thf cyclohydrolase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, nad-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase, dhch1, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5,10-methenyl-H4folate cyclohydrolase
-
-
-
0
Citrovorum factor cyclodehydrase
-
-
-
0
cyclohydrolase
-
-
-
0
formyl-methenyl-methylenetetrahydrofolate synthetase (combined)
-
-
-
0
methenyl-THF cyclohydrolase
-
-
-
0
N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase
bifunctional enzyme
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amidine hydrolysis
-
-
-
0
SYSTEMATIC NAME
IUBMB Comments
5,10-methenyltetrahydrofolate 5-hydrolase (decyclizing)
In eukaryotes, the enzyme occurs as a trifunctional enzyme that also has methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) and formate---tetrahydrofolate ligase (EC 6.3.4.3) activity. In some prokaryotes, it occurs as a bifunctional enzyme that also has dehydrogenase (EC 1.5.1.5) activity or formimidoyltetrahydrofolate cyclodeaminase (EC 4.3.1.4) activity.
CAS REGISTRY NUMBER
COMMENTARY hide
9027-97-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,10-methenyltetrahydrofolate + H2O
10-formyltetrahydrofolate + H+
show the reaction diagram
-
-
0
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5,10-methenyltetrahydrofolate + H2O
10-formyltetrahydrofolate + H+
show the reaction diagram
-
-
-
r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-[(2-chloro-6-fluorobenzyl)sulfanyl]-6-(trifluoromethyl)pyrimidin-4-ol
-
5-[(4-chlorophenyl)sulfonyl]-6-ethoxypyrimidine-2,4-diamine
-
6-benzyl-5-methyl-7-oxo-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile
-
LY354899
i.e. 5,6,7,8-tetrahydro-N5,N10-carbonylfolic acid
LY374571
i.e. (2R)-2-[(4-{[(2,5-diamino-6-hydroxypyrimidin-4-yl)carbamoyl]amino}phenyl)formamido]pentanedioic acid
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.026
5,10-methenyltetrahydrofolate
at pH 7.9 and 22°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0025
2-[(2-chloro-6-fluorobenzyl)sulfanyl]-6-(trifluoromethyl)pyrimidin-4-ol
Pseudomonas aeruginosa
at pH 7.9 and 22°C
0.0009
5-[(4-chlorophenyl)sulfonyl]-6-ethoxypyrimidine-2,4-diamine
Pseudomonas aeruginosa
at pH 7.9 and 22°C
0.0038
6-benzyl-5-methyl-7-oxo-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile
Pseudomonas aeruginosa
at pH 7.9 and 22°C
0.00003
LY374571
Pseudomonas aeruginosa
at pH 7.9 and 22°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A485FWC5_PSEAI
103
0
11022
TrEMBL
-
A0A2S5II11_PSEAI
284
0
30550
TrEMBL
-
A0A5M6GQS0_PSEAI
98
0
10660
TrEMBL
-
A0A6G7LCL4_PSEAI
284
0
30563
TrEMBL
-
A0A3A2CPG3_PSEAI
284
0
30553
TrEMBL
-
A0A2K4XIL5_PSEAI
291
0
30161
TrEMBL
-
A0A071LH88_PSEAI
284
0
30549
TrEMBL
-
A0A485FY06_PSEAI
188
0
20441
TrEMBL
-
A0A2R3ILU4_PSEAI
284
0
30519
TrEMBL
-
A0A431X8U0_PSEAI
306
0
32276
TrEMBL
-
A0A367LZV9_PSEAI
226
0
24345
TrEMBL
-
A0A2V3GGW1_PSEAI
123
0
13478
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
2 * 30000, SDS-PAGE
60000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 30000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 25% (w/v) PEG 3350 and 0.2 M magnesium formate
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+-charged HisTrap column chromatography and Superdex 200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Eadsforth, T.C.; Gardiner, M.; Maluf, F.V.; McElroy, S.; James, D.; Frearson, J.; Gray, D.; Hunter, W.N.
Assessment of Pseudomonas aeruginosa N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase as a potential antibacterial drug target
PLoS ONE
7
e35973
2012
Pseudomonas aeruginosa (Q9I2U6), Pseudomonas aeruginosa
Manually annotated by BRENDA team
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