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Information on EC 3.5.3.17 - Guanidinopropionase
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3.5.3.17 GuanidinopropionaseIUBMB Comments
Requires Mn2+. Also acts, more slowly, on taurocyamine and 4-guanidinobutanoate.
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Word Map
- 3.5.3.17
- glycogen
- phosphorylase
-
glycogenolysis
- gsase
- fbpase
- 4-guanidinobutyrate
- 3-guanidinopropionate
-
diapause
The enzyme appears in viruses and cellular organisms
Synonyms
gpase, guanidinopropionase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
guanidinopropionase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3-Guanidinopropanoate + H2O = beta-alanine + urea
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SYSTEMATIC NAME
IUBMB Comments
3-Guanidinopropanoate amidinopropionase
Requires Mn2+. Also acts, more slowly, on taurocyamine and 4-guanidinobutanoate.
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CAS REGISTRY NUMBER
COMMENTARY
68821-77-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-Guanidinopropanoate + H2O
beta-Alanine + urea
-
highly specific for
-
?
3-Guanidinopropanoate + H2O
beta-Alanine + urea
-
highly specific for
-
?
3-Guanidinopropanoate + H2O
beta-Alanine + urea
-
highly specific for
-
?
4-Guanidinobutyrate + H2O
4-Aminobutyrate + urea
-
3% of the activity with 3-guanidinopropanoate
-
?
4-Guanidinobutyrate + H2O
4-Aminobutyrate + urea
-
3% of the activity with 3-guanidinopropanoate
-
?
Taurocyamine + H2O
?
-
11% of the activity with 3-guanidinopropanoate
-
?
Taurocyamine + H2O
?
-
11% of the activity with 3-guanidinopropanoate
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
-
purified native enzyme is fully active without added divalent metal, most potent activator of EDTA-treated enzyme
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
12.8
3-guanidinopropionate
pH 7.9, 30°C, wild-type unmethylated GpuA
108
3-guanidinopropionate
pH 7.9, 30°C, mutant Y157M unmethylated GpuA
37.3
4-Guanidinobutyrate
pH 7.9, 30°C, wild-type unmethylated GpuA
285
4-Guanidinobutyrate
pH 7.9, 30°C, mutant Y157M unmethylated GpuA
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
48.8
3-guanidinopropionate
pH 7.9, 30°C, mutant Y157M unmethylated GpuA
106
3-guanidinopropionate
pH 7.9, 30°C, wild-type unmethylated GpuA
2 - 8
4-Guanidinobutyrate
pH 7.9, 30°C, mutant Y157M unmethylated GpuA
57.6
4-Guanidinobutyrate
pH 7.9, 30°C, wild-type unmethylated GpuA
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8.28
3-guanidinopropionate
pH 7.9, 30°C, wild-type unmethylated GpuA
1.54
4-Guanidinobutyrate
pH 7.9, 30°C, wild-type unmethylated GpuA
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain PAO 1 (wild-type and mutant strain M-6, capable of utilizing taurocyamine as nitrogen source, with altered induction of the enzyme)
-
-
brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GPUA_PSEAE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
318
0
34189
Swiss-Prot
-
A0A2S6SFF6_9PROT
313
0
34799
TrEMBL
-
A0A2S6SUS5_9PROT
324
0
35673
TrEMBL
-
A0A5B8RG06_9ZZZZ
354
0
38005
TrEMBL
other Location (Reliability: 2)
A0A1U7M7Z7_TISCR
319
0
35158
TrEMBL
-
A0A2Z2NW38_9GAMM
323
0
35429
TrEMBL
-
A0A2S6SAS8_9PROT
317
0
34900
TrEMBL
-
A0A2S6T093_9PROT
313
0
34962
TrEMBL
-
A0A2Z2NTG2_9GAMM
324
0
35166
TrEMBL
-
A0A2S6SVS7_9PROT
327
0
36544
TrEMBL
-
A0A259UJS8_9FIRM
335
0
37190
TrEMBL
-
A0A2S6T1D1_9PROT
317
0
35193
TrEMBL
-
A0A2S6S798_9PROT
311
0
34655
TrEMBL
-
A0A2S6R0P6_9PROT
323
0
35373
TrEMBL
-
A0A644TP46_9ZZZZ
317
0
34453
TrEMBL
Mitochondrion (Reliability: 5)
A0A380TAF6_9ZZZZ
303
0
31705
TrEMBL
other Location (Reliability: 5)
A0A221K098_9RHOB
317
0
33799
TrEMBL
-
A0A2S6SEE2_9PROT
317
0
35193
TrEMBL
-
A0A2S6SUV8_9PROT
312
0
34906
TrEMBL
-
A0A564WEN9_9PROT
303
0
31705
TrEMBL
-
A0A2S6ULA3_9PROT
324
0
34854
TrEMBL
-
A0A2S6SWV4_9PROT
317
0
34900
TrEMBL
-
A0A644WQR1_9ZZZZ
331
0
35831
TrEMBL
other Location (Reliability: 3)
Q4KJW3_PSEF5
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
318
0
34218
TrEMBL
-
A0A6A7SJS9_9ZZZZ
354
0
38005
TrEMBL
other Location (Reliability: 2)
A0A2S6S889_9PROT
330
0
36976
TrEMBL
-
A0A399F5C1_9DEIN
316
0
35246
TrEMBL
-
A0A1Z5HDB5_9GAMM
336
0
36996
TrEMBL
-
A0A259U4J4_9FIRM
333
0
36016
TrEMBL
-
A0A2S6T7L9_9PROT
313
0
34962
TrEMBL
-
A0A2S6SMT7_9PROT
313
0
34986
TrEMBL
-
A0A2S6UVV3_9PROT
318
0
34285
TrEMBL
-
A0A2S6T0R6_9PROT
313
0
34799
TrEMBL
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
195000 - 215000
-
Pseudomonas aeruginosa, gel filtration of crystalline enzyme, disc gel electrophoresis
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structures are determined of Mn2+-bound GpuA (methylated), and 1,6-diaminohexane-bound GpuA (unmethylated)
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Y157M
Km for substrates 3-guanidinopropionate or 4-guanidinobutyrate significantly increased compared to wild-type, kcat for substrates 3-guanidinopropionate or 4-guanidinobutyrate significantly decreased
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM Tris-chloride buffer, pH 8.0, 0.05 mM MnCl2, 25% glycerol, partially purified enzyme is stable for up to 5 months
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
mutant strain M-6, capable of utilizing taurocyamine as nitrogen source
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yorifuji, T.; Sugai, I.; Matsumoto, H.; Tabuchi, A.
Characterization of 3-guanidinopropionate amidinohydrolase from Pseudomonas aeruginosa and a comparative study with 4-guanidinobutyrate amidinohydrolase from another Pseudomonas
Agric. Biol. Chem.
46
1361-1367
1982
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO 1
-
brenda
Yorifuji, T.; Sugai, I.
3-Guanidinopropionate amidinohydrolase and 4-guanidinobutyrate amidinohydrolase of Pseudomonas aeruginosa strain PAO1
Agric. Biol. Chem.
42
1789-1790
1978
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO 1
-
brenda
Yorifuji, T.; Kobayashi, T.; Tabuchi, A.; Shiritani, Y.; Yonaha, K.
Distribution of amidinohydrolases among Pseudomonas and comparative studies of some purified enzymes by one-dimensional peptide mapping
Agric. Biol. Chem.
47
2825-2830
1983
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO 1
-
brenda
Yorifuji, T.; Sawada, K.; Tokuda, C.
Taurocyamine-utilizing mutant of Pseudomonas aeruginosa with altered induction of 3-guanidinopropionate amidinohydrolase
Agric. Biol. Chem.
50
3077-3082
1986
Pseudomonas aeruginosa
-
brenda
Nakada, Y.; Itoh, Y.
Pseudomonas aeruginosa PAO1 genes for 3-guanidinopropionate and 4-guanidinobutyrate utilization may be derived from a common ancestor
Microbiology
151
4055-4062
2005
Pseudomonas aeruginosa
brenda
Lee, S.J.; Kim, d.o..J.; Kim, H.S.; Lee, B.I.; Yoon, H.J.; Yoon, J.Y.; Kim, K.H.; Jang, J.Y.; Im, H.N.; An, D.R.; Song, J.S.; Kim, H.J.; Suh, S.W.
Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily
J. Struct. Biol.
175
329-338
2011
Pseudomonas aeruginosa (Q9I6K2), Pseudomonas aeruginosa
brenda
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