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EC Tree
The expected taxonomic range for this enzyme is: Pseudomonas
Synonyms
amidinoaspartic amidinohydrolase, Mn2+-dependent amidinoaspartase, N-amidino-L-aspartic acid amidinohydrolase,
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amidinoaspartic amidinohydrolase
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Mn2+-dependent amidinoaspartase
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N-amidino-L-aspartic acid amidinohydrolase
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N-amidino-L-aspartate + H2O = L-aspartate + urea
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N-amidino-L-aspartate amidinohydrolase
Also acts slowly on N-amidino-L-glutamate.
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guanidinoglutaric acid + H2O
?
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-
?
N-amidino-L-aspartic acid
L-aspartic acid + urea
N-amidino-L-aspartic acid + H2O
L-aspartic acid + urea
N-amidino-L-glutamate + H2O
L-glutamic acid + urea
additional information
?
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N-amidino-L-aspartic acid
L-aspartic acid + urea
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?
N-amidino-L-aspartic acid
L-aspartic acid + urea
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?
N-amidino-L-aspartic acid
L-aspartic acid + urea
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?
N-amidino-L-aspartic acid + H2O
L-aspartic acid + urea
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?
N-amidino-L-aspartic acid + H2O
L-aspartic acid + urea
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?
N-amidino-L-aspartic acid + H2O
L-aspartic acid + urea
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?
N-amidino-L-glutamate + H2O
L-glutamic acid + urea
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?
N-amidino-L-glutamate + H2O
L-glutamic acid + urea
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?
additional information
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enzyme is specific for the L-isomer
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additional information
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enzyme is specific for the L-isomer
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additional information
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N-amidino-D-aspartate, 3-guanidinopropionic acid, 4-guanidinobutyric acid, 2-amino-4-guanidinopropionic acid, agmatine, arginine, N-acetyl-arginine, creatinine, guanidinoacetic acid are no substrates
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?
additional information
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additional information
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C-32, guanidinoacetate, 3-guanidinopropionate, 4-guanidinobutyrate, 5-guanidinovalerate, 6-guanidinocaproate, 2-guanidinoethylsulfate, taurocyamine, 4-guanidino-3-hydroxybutyrate, L-arginine, DL-N-acetylarginine, DL-2-guanidinoisobutyrate, N-amidinoalanine, methylguanidine, guanidine and creatine are no substrates
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?
additional information
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C-32, guanidinoacetate, 3-guanidinopropionate, 4-guanidinobutyrate, 5-guanidinovalerate, 6-guanidinocaproate, 2-guanidinoethylsulfate, taurocyamine, 4-guanidino-3-hydroxybutyrate, L-arginine, DL-N-acetylarginine, DL-2-guanidinoisobutyrate, N-amidinoalanine, methylguanidine, guanidine and creatine are no substrates
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?
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N-amidino-L-aspartic acid
L-aspartic acid + urea
N-amidino-L-aspartic acid
L-aspartic acid + urea
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?
N-amidino-L-aspartic acid
L-aspartic acid + urea
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?
N-amidino-L-aspartic acid
L-aspartic acid + urea
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?
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Mn2+
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C-32, specifically activation
Co2+
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Co2+
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temperature-dependent activation
Co2+
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C-32, 27% of the activation with Mn2+
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aspartic acid
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competitive inhibition
Urea
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competitive inhibition
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0.0094 - 20
N-amidino-L-aspartate
0.0094
N-amidino-L-aspartate
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C-32, activation with Mn2+
0.21
N-amidino-L-aspartate
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C-32 , activation with Co2+
2.4
N-amidino-L-aspartate
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HEPES buffer, pH 8, assay method B
8.3
N-amidino-L-aspartate
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HEPES buffer, pH 8, assay method A
10.3
N-amidino-L-aspartate
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C-32
20
N-amidino-L-aspartate
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phosphate buffer
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no activity in Pseudomonas chlororaphis
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brenda
no activity in Pseudomonas chlororaphis ATCC 9446
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brenda
fluorescent Pseudomonas
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brenda
C-32
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brenda
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brenda
C-32
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brenda
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240000
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C-32, gel filtration
39800
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5 * 39800, C-32, SDS-PAGE
300000
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pentamer
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5 * 39800, C-32, SDS-PAGE
pentamer
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5 * 39800, C-32, SDS-PAGE
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40 - 50
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incubation at 40°C results in rapid activation, prolonged incubation causes irreversible loss of activity, incubation at 50°C 90% of the initial enzyme activity lost within 10 min
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-15°C, stable for several months
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medicine
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test for N-amidino-L-aspartate in the urine of uremic patients
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Milstien, S.; Goldman, P.
Metabolism of guanidinosuccinic acid. I. Characterization of a specific amidinohydrolase from Pseudomonas chlororaphis
J. Biol. Chem.
247
6280-6283
1972
Pseudomonas putida, Pseudomonas chlororaphis
brenda
Yorifuji, T.; Furuyoshi, S.
Mn2+ dependent amidinoaspartase in Pseudomonas putida
Agric. Biol. Chem.
50
1327-1328
1986
no activity in Pseudomonas chlororaphis, Pseudomonas putida, Pseudomonas chlororaphis, no activity in Pseudomonas chlororaphis ATCC 9446, Pseudomonas putida C-32
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brenda
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