We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The expected taxonomic range for this enzyme is: Pseudomonas
Synonyms
amidinoaspartic amidinohydrolase, Mn2+-dependent amidinoaspartase, N-amidino-L-aspartic acid amidinohydrolase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
amidinoaspartic amidinohydrolase
-
-
-
-
Mn2+-dependent amidinoaspartase
-
-
N-amidino-L-aspartic acid amidinohydrolase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
N-amidino-L-aspartate + H2O = L-aspartate + urea
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
amidine hydrolysis
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
N-amidino-L-aspartate amidinohydrolase
Also acts slowly on N-amidino-L-glutamate.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
guanidinoglutaric acid + H2O
?
-
-
-
-
?
N-amidino-L-aspartic acid
L-aspartic acid + urea
N-amidino-L-aspartic acid + H2O
L-aspartic acid + urea
N-amidino-L-glutamate + H2O
L-glutamic acid + urea
additional information
?
-
N-amidino-L-aspartic acid
L-aspartic acid + urea
-
-
-
-
?
N-amidino-L-aspartic acid
L-aspartic acid + urea
-
-
-
-
?
N-amidino-L-aspartic acid
L-aspartic acid + urea
-
-
-
-
?
N-amidino-L-aspartic acid + H2O
L-aspartic acid + urea
-
-
-
-
?
N-amidino-L-aspartic acid + H2O
L-aspartic acid + urea
-
-
-
-
?
N-amidino-L-aspartic acid + H2O
L-aspartic acid + urea
-
-
-
-
?
N-amidino-L-glutamate + H2O
L-glutamic acid + urea
-
-
-
-
?
N-amidino-L-glutamate + H2O
L-glutamic acid + urea
-
-
-
-
?
additional information
?
-
-
enzyme is specific for the L-isomer
-
-
?
additional information
?
-
-
enzyme is specific for the L-isomer
-
-
?
additional information
?
-
-
N-amidino-D-aspartate, 3-guanidinopropionic acid, 4-guanidinobutyric acid, 2-amino-4-guanidinopropionic acid, agmatine, arginine, N-acetyl-arginine, creatinine, guanidinoacetic acid are no substrates
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
C-32, guanidinoacetate, 3-guanidinopropionate, 4-guanidinobutyrate, 5-guanidinovalerate, 6-guanidinocaproate, 2-guanidinoethylsulfate, taurocyamine, 4-guanidino-3-hydroxybutyrate, L-arginine, DL-N-acetylarginine, DL-2-guanidinoisobutyrate, N-amidinoalanine, methylguanidine, guanidine and creatine are no substrates
-
-
?
additional information
?
-
-
C-32, guanidinoacetate, 3-guanidinopropionate, 4-guanidinobutyrate, 5-guanidinovalerate, 6-guanidinocaproate, 2-guanidinoethylsulfate, taurocyamine, 4-guanidino-3-hydroxybutyrate, L-arginine, DL-N-acetylarginine, DL-2-guanidinoisobutyrate, N-amidinoalanine, methylguanidine, guanidine and creatine are no substrates
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
N-amidino-L-aspartic acid
L-aspartic acid + urea
N-amidino-L-aspartic acid
L-aspartic acid + urea
-
-
-
-
?
N-amidino-L-aspartic acid
L-aspartic acid + urea
-
-
-
-
?
N-amidino-L-aspartic acid
L-aspartic acid + urea
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mn2+
-
C-32, specifically activation
Co2+
-
-
Co2+
-
temperature-dependent activation
Co2+
-
C-32, 27% of the activation with Mn2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
aspartic acid
-
competitive inhibition
Urea
-
competitive inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0094 - 20
N-amidino-L-aspartate
0.0094
N-amidino-L-aspartate
-
C-32, activation with Mn2+
0.21
N-amidino-L-aspartate
-
C-32 , activation with Co2+
2.4
N-amidino-L-aspartate
-
HEPES buffer, pH 8, assay method B
8.3
N-amidino-L-aspartate
-
HEPES buffer, pH 8, assay method A
10.3
N-amidino-L-aspartate
-
C-32
20
N-amidino-L-aspartate
-
phosphate buffer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
no activity in Pseudomonas chlororaphis
-
-
-
brenda
no activity in Pseudomonas chlororaphis ATCC 9446
-
-
-
brenda
fluorescent Pseudomonas
-
-
brenda
C-32
-
-
brenda
-
-
-
brenda
C-32
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
240000
-
C-32, gel filtration
39800
-
5 * 39800, C-32, SDS-PAGE
300000
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pentamer
-
5 * 39800, C-32, SDS-PAGE
pentamer
-
5 * 39800, C-32, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
40 - 50
-
incubation at 40°C results in rapid activation, prolonged incubation causes irreversible loss of activity, incubation at 50°C 90% of the initial enzyme activity lost within 10 min
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-15°C, stable for several months
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
medicine
-
test for N-amidino-L-aspartate in the urine of uremic patients
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Milstien, S.; Goldman, P.
Metabolism of guanidinosuccinic acid. I. Characterization of a specific amidinohydrolase from Pseudomonas chlororaphis
J. Biol. Chem.
247
6280-6283
1972
Pseudomonas chlororaphis, Pseudomonas putida
brenda
Yorifuji, T.; Furuyoshi, S.
Mn2+ dependent amidinoaspartase in Pseudomonas putida
Agric. Biol. Chem.
50
1327-1328
1986
no activity in Pseudomonas chlororaphis, Pseudomonas putida, Pseudomonas chlororaphis, no activity in Pseudomonas chlororaphis ATCC 9446, Pseudomonas putida C-32
-
brenda
Select items on the left to see more content.
html completed
BRENDA home
History
3.5.3.14 amidinoaspartase
more
top
