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Information on EC 3.5.1.74 - chenodeoxycholoyltaurine hydrolase
for references in articles please use BRENDA:EC3.5.1.74
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EC Tree 3 Hydrolases
3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
3.5.1 In linear amides
3.5.1.74 chenodeoxycholoyltaurine hydrolase
IUBMB Comments
Some other taurine conjugates are hydrolysed, but not glycine conjugates of bile acids.
The enzyme appears in viruses and cellular organisms
showSynonyms
CTH, hydrolase, chenodeoxychoyltaurine, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CTH
hydrolase, chenodeoxychoyltaurine
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
chenodeoxycholoyltaurine + H2O = chenodeoxycholate + taurine
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of linear amides
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
chenodeoxycholoyltaurine amidohydrolase
Some other taurine conjugates are hydrolysed, but not glycine conjugates of bile acids.
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CAS REGISTRY NUMBER
COMMENTARY
125752-75-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
tauro (3alpha,5beta,7beta,12alpha)-3,7,12-trihydroxycholan-24-oic amide + H2O
taurine + (3alpha,5beta,7beta,12alpha)-3,7,12-trihydroxycholan-24-oic acid
-
Substrates: 0.5% of activity compared to taurochenodeoxycholic acid
Products: -
Products: -
?
tauro-beta-muricholic acid + H2O
taurine + beta-muricholic acid
-
Substrates: 62% of activity compared to taurochenodeoxycholic acid
Products: -
Products: -
?
taurocholic acid + H2O
taurine + cholic acid
-
Substrates: 2.8% of activity compared to taurochenodeoxycholic acid
Products: -
Products: -
?
taurodeoxycholic acid + H2O
taurine + deoxycholic acid
-
Substrates: 31% of activity compared to taurochenodeoxycholic acid
Products: -
Products: -
?
taurohyocholic acid + H2O
taurine + hyocholic acid
-
Substrates: 24% of activity compared to taurochenodeoxycholic acid
Products: -
Products: -
?
taurohyodeoxycholic acid + H2O
taurine + hyodeoxycholic acid
-
Substrates: 36% of activity compared to taurochenodeoxycholic acid
Products: -
Products: -
?
tauroursodeoxycholic acid + H2O
taurine + ursodeoxycholic acid
-
Substrates: 56% of activity compared to taurochenodeoxycholic acid
Products: -
Products: -
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
-
Substrates: best substrate
Products: -
Products: -
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
Phocaeicola vulgatus VI-31
-
Substrates: best substrate
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificity
Products: -
Products: -
?
additional information
?
-
-
Substrates: hydrolyzes taurine-conjugated bile acids, glycine-conjugated bile acids are no substrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with glycocholic acid, glyco-7beta-cholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, glyco-beta-muricholic acid, glycoursodeoxycholic acid, glycohyocholic acid, glycohyodeoxycholic acid
Products: -
Products: -
?
additional information
?
-
-
Substrates: specificity for not only the amino acid moiety but also the steroidal moiety
Products: -
Products: -
?
additional information
?
-
Phocaeicola vulgatus VI-31
-
Substrates: substrate specificity
Products: -
Products: -
?
additional information
?
-
Phocaeicola vulgatus VI-31
-
Substrates: hydrolyzes taurine-conjugated bile acids, glycine-conjugated bile acids are no substrates
Products: -
Products: -
?
additional information
?
-
Phocaeicola vulgatus VI-31
-
Substrates: no activity with glycocholic acid, glyco-7beta-cholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, glyco-beta-muricholic acid, glycoursodeoxycholic acid, glycohyocholic acid, glycohyodeoxycholic acid
Products: -
Products: -
?
additional information
?
-
Phocaeicola vulgatus VI-31
-
Substrates: specificity for not only the amino acid moiety but also the steroidal moiety
Products: -
Products: -
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
dithiodipyridine
-
0.1 mM: 15.9% inhibition, 1 mM: 50.2% inhibition, 10 mM: complete inhibition
N-ethylmaleimide
-
0.1 mM: 3.5% inhibition, 1 mM: 27.4% inhibition, 10 mM: 90.8% inhibition
p-hydroxymercuribenzoate
-
0.00001 mM: 4% inhibition, 0.0001 mM: 60% inhibition, 0.001 mM: complete inhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A087AZ12_BIFLL
317
0
35125
TrEMBL
-
A0A087BL81_BIFLN
317
0
35143
TrEMBL
-
A0A0A1GNM5_BIFLN
317
0
35155
TrEMBL
Secretory Pathway (Reliability: 2)
A0A0H3ECC2_BIFBP
Bifidobacterium bifidum (strain PRL2010)
316
0
35258
TrEMBL
-
A0A0L7CW47_BIFBR
317
0
35125
TrEMBL
-
A0A0L7AX86_BIFBR
317
0
35124
TrEMBL
-
A0A0M4MFZ9_BIFLI
317
0
35098
TrEMBL
-
A0A286TEZ2_BIFBI
316
0
35260
TrEMBL
-
A0A4R0SBQ2_BIFLL
317
0
35097
TrEMBL
-
A0A4R0U1X2_BIFLL
317
0
35097
TrEMBL
-
A0A4R0V6Y8_BIFLL
317
0
35111
TrEMBL
-
A0A4S5BBQ1_BIFLI
317
0
35124
TrEMBL
-
A0A4T0UI03_BIFLI
317
0
35154
TrEMBL
-
A0A564S0A5_BIFLI
317
0
35168
TrEMBL
-
A0A837U229_BIFLL
317
0
35143
TrEMBL
-
A0A8U0KVA1_BIFLI
317
0
35080
TrEMBL
Secretory Pathway (Reliability: 1)
A0AA46JXR4_BIFLL
317
0
35273
TrEMBL
other Location (Reliability: 3)
A0AA87IE42_BIFLL
317
0
35125
TrEMBL
-
A0AAJ1UN94_9BIFI
316
0
35319
TrEMBL
-
A0AAV3FLN3_BIFLL
317
0
35125
TrEMBL
other Location (Reliability: 4)
A0AAX1LIP7_BIFLI
317
0
35138
TrEMBL
-
A0AB74HH92_BIFLL
317
0
35099
TrEMBL
Mitochondrion (Reliability: 3)
B3DS63_BIFLD
Bifidobacterium longum (strain DJO10A)
317
0
35125
TrEMBL
-
B7GRV6_BIFLS
Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
317
0
35101
TrEMBL
-
C5EAY9_BIFLI
317
0
35125
TrEMBL
other Location (Reliability: 5)
D4BPE8_BIFBR
334
0
36999
TrEMBL
-
D6ZU57_BIFLJ
Bifidobacterium longum subsp. longum (strain JDM301)
317
0
35143
TrEMBL
-
Q6J264_BIFBI
316
0
35288
TrEMBL
other Location (Reliability: 3)
Q8G653_BIFLO
Bifidobacterium longum (strain NCC 2705)
317
0
35125
TrEMBL
-
CBH_BIFLN
317
0
35155
Swiss-Prot
other Location (Reliability: 5)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
tetramer
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kawamoto, K.; Horibe, I.; Uchida, K.
Purification and characterization of a new hydrolase for conjugated bile acids, chenodeoxycholyltaurine hydrolase, from Bacteroides vulgatus
J. Biochem.
106
1049-1053
1989
Phocaeicola vulgatus, Phocaeicola vulgatus VI-31
brenda
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