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Information on EC 3.5.1.56 - N,N-dimethylformamidase
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3.5.1.56 N,N-dimethylformamidaseIUBMB Comments
An iron protein. Also acts on N-ethylformamide and N-methyl-formamide and, more slowly, on N,N-diethylformamide, N,N-dimethylacetamide and unsubstituted acyl amides.
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Word Map
- 3.5.1.56
- paracoccus
-
methylotrophic
- waste
- alcaligenes
- aminophilus
- environmental protection
The enzyme appears in viruses and cellular organisms
Synonyms
dmfase, dimethylformamidase, n,n-dimethylformamidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
dimethylformamidase
DMFase
N,N-dimethylformamidase
dimethylformamidase
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-
-
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DMFase
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N,N-dimethylformamide + H2O = dimethylamine + formate
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-
-
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N,N-dimethylformamide + H2O = dimethylamine + formate
an enzyme of high substrate specificity that catalyzes the first step in the degradation of N,N-dimethylformamide
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of linear amides
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-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
N,N-dimethylformamide amidohydrolase
An iron protein. Also acts on N-ethylformamide and N-methyl-formamide and, more slowly, on N,N-diethylformamide, N,N-dimethylacetamide and unsubstituted acyl amides.
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CAS REGISTRY NUMBER
COMMENTARY
104645-73-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
formamide + H2O
NH3 + formate
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3.8% of the activity with N,N-dimethylformamide
-
?
prolinamide + H2O
NH3 + proline
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1.6% of the activity with N,N-dimethylformamide
-
?
N,N-diethylformamide + H2O
diethylamine + formate
-
29% of the activity with N,N-dimethylformamide
-
?
N,N-diethylformamide + H2O
diethylamine + formate
-
29% of the activity with N,N-dimethylformamide
-
?
N,N-diethylformamide + H2O
diethylamine + formate
-
2.6% of the activity with N,N-dimethylformamide
-
?
N,N-diethylformamide + H2O
diethylamine + formate
-
2.6% of the activity with N,N-dimethylformamide
-
?
N,N-dimethylacetamide + H2O
dimethylamine + acetate
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31% of the activity with N,N-dimethylformamide
-
?
N,N-dimethylacetamide + H2O
dimethylamine + acetate
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31% of the activity with N,N-dimethylformamide
-
?
N,N-dimethylacetamide + H2O
dimethylamine + acetate
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3.4% of the activity with N,N-dimethylformamide
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?
N,N-dimethylformamide + H2O
dimethylamine + formate
A0A2Y9CJ10
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-
?
N,N-dimethylformamide + H2O
dimethylamine + formate
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catabolic pathway and related enzymes, degradation by strain DGVK1 leads to accumulation of ammonia and dimethylamine, overview
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-
?
N,N-dimethylformamide + H2O
dimethylamine + formate
-
catabolic pathway and related enzymes, degradation by strain DGVK1 leads to accumulation of ammonia and dimethylamine, overview
-
-
?
N,N-dimethylformamide + H2O
dimethylamine + formate
-
an enzyme of high substrate specificity that catalyzes the first step in the degradation of N,N-dimethylformamide
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-
?
N,N-dimethylformamide + H2O
dimethylamine + formate
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N,N-dimethylformamide, a toxic solvent used in the chemical industry, is frequently present in industrial waste
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-
?
N-ethylformamide + H2O
ethylamine + formate
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31.5% of the activity with N,N-dimethylformamide
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?
N-ethylformamide + H2O
ethylamine + formate
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31.5% of the activity with N,N-dimethylformamide
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?
N-methylformamide + H2O
methylamine + formate
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14.8% of the activity with N,N-dimethylformamide
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?
N-methylformamide + H2O
methylamine + formate
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14.8% of the activity with N,N-dimethylformamide
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?
additional information
?
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less than 1% of the activity with N,N-dimethylformamide: acetamide, acrylamide, butyramide
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?
additional information
?
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less than 1% of the activity with N,N-dimethylformamide: acetamide, acrylamide, butyramide
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N,N-dimethylformamide + H2O
dimethylamine + formate
A0A2Y9CJ10
-
-
-
?
N,N-dimethylformamide + H2O
dimethylamine + formate
-
catabolic pathway and related enzymes, degradation by strain DGVK1 leads to accumulation of ammonia and dimethylamine, overview
-
-
?
N,N-dimethylformamide + H2O
dimethylamine + formate
-
catabolic pathway and related enzymes, degradation by strain DGVK1 leads to accumulation of ammonia and dimethylamine, overview
-
-
?
N,N-dimethylformamide + H2O
dimethylamine + formate
-
an enzyme of high substrate specificity that catalyzes the first step in the degradation of N,N-dimethylformamide
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
27.6
N,N-Dimethylformamide
A0A2Y9CJ10
pH 6.5, 37°C, recombinant enzyme
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isolated from soil samples collected from coalmine leftovers
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
A0A2Y9CJ10
strain DM1 can utilize N,N-dimethylformamide, DMA, MA, methanol, ethanol, formamide, toluene, glycerol, and trisodium citrate as the sole source of carbon or carbon and nitrogen
brenda
additional information
-
strain DGVK1 is able to grow on N,N-dimethylformamide as sole carbon and nitrogen source, overview
brenda
additional information
-
strain DGVK1 is able to grow on N,N-dimethylformamide as sole carbon and nitrogen source, overview
-
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
A0A2Y9CJ10
the enzyme is important and initial in the N,N-dimethylformamide (DMF) degradation pathway, transcriptional analysis of genes specific to DMF degradation, overview
physiological function
physiological function
-
plasmid pAMI2 of Paracoccus aminophilus JCM 7686 carries genetic information which is crucial for the methylotrophic growth, using N,N-dimethylformamide as the sole source of carbon and energy. The plasmid carries a three-gene cluster coding for DmfR, a transcription regulator of the LuxR familiy and two subunits, DmfA1 and DmfA2 of N,N-dimethylformamidase, an enzyme of high substrate specificity that catalyzes the first step in the degradation of N,N-dimethylformamide
physiological function
A0A2Y9CJ10
the enzyme is responsible for N,N-dimethylformamide degradation, molecular mechanism, overview
Show AA Sequence and Transmembrane Helices (156 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
heterotetramer
A0A2Y9CJ10
recombinent enzyme, alpha2beta2, 2 * 20000, alpha-subunit, + 2 * 80000, beta-subunit, SDS-PAGE
tetramer
additional information
additional information
-
plasmid pAMI2 of Paracoccus aminophilus JCM 7686 carries genetic information which is crucial for the methylotrophic growth, using N,N-dimethylformamide as the sole source of carbon and energy. The plasmid carries a three-gene cluster coding for DmfR, a transcription regulator of the LuxR familiy and two subunits, DmfA1 and DmfA2 of N,N-dimethylformamidase, an enzyme of high substrate specificity that catalyzes the first step in the degradation of N,N-dimethylformamide
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
A0A2Y9CJ10
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Escherichai coli
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encoded on plasmid pLVM1, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
A0A2Y9CJ10
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
environmental protection
environmental protection
-
the enzyme activity is useful to treat industrial effluent containing dimethylformamide obtained from pharmaceutical industry
environmental protection
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the enzyme activity is useful to treat industrial effluent containing dimethylformamide obtained from pharmaceutical industry
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schr, H.P.; Holzmann, W.; Ramos Tombo, G.M.; Ghisalba, O.
Purification and characterization of N,N-dimethylformamidase from Pseudomonas DMF 3/3
Eur. J. Biochem.
158
469-475
1986
Pseudomonas sp., Pseudomonas sp. DMF 3/3
brenda
Hasegawa, Y.; Matsuo, M.; Sigemoto, Y.; Sakai, T.; Tokuyama, T.
Purification and characterisation of N,N-dimethylformamidase from Alcaligenes sp. KUFA-1
J. Ferment. Bioeng.
84
543-547
1997
Alcaligenes sp., Alcaligenes sp. KUFA-1
-
brenda
Veeranagouda, Y.; Emmanuel Paul, P.V.; Gorla, P.; Siddavattam, D.; Karegoudar, T.B.
Complete mineralisation of dimethylformamide by Ochrobactrum sp. DGVK1 isolated from the soil samples collected from the coalmine leftovers
Appl. Microbiol. Biotechnol.
71
369-375
2006
Ochrobactrum sp., Ochrobactrum sp. DGVK1
brenda
Dziewit, L.; Dmowski, M.; Baj, J.; Bartosik, D.
Plasmid pAMI2 of Paracoccus aminophilus JCM 7686 carries N,N-dimethylformamide degradation-related genes whose expression is activated by a LuxR family regulator
Appl. Environ. Microbiol.
76
1861-1869
2010
Paracoccus aminophilus
brenda
Lu, X.; Wang, W.; Zhang, L.; Hu, H.; Xu, P.; Wei, T.; Tang, H.
Molecular mechanism of N,N-dimethylformamide degradation in Methylobacterium sp. strain DM1
Appl. Environ. Microbiol.
85
e00275-19
2019
Methylobacterium sp. DM1 (A0A2Y9CJ10)
brenda
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