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IUBMB CommentsAlso acts on acetylagmatine, propanoylagmatine and bleomycin B2
The expected taxonomic range for this enzyme is: Fusarium
Synonyms
acylagmatine amidohydrolase,
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acylagmatine amidohydrolase
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acylagmatine deacylase
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benzoylagmatine + H2O = benzoate + agmatine
Also acts on acetylagmatine, propanoylagmatine and bleomycin B2
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hydrolysis of peptide bond
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benzoylagmatine amidohydrolase
Also acts on acetylagmatine, propanoylagmatine and bleomycin B2
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acetyl agmatine + H2O
acetic acid + agmatine
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benzoyl agmatine + H2O
benzoic acid + agmatine
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bleomycin B2 + H2O
bleomycinic acid + agmatine
propionyl agmatine + H2O
propionic acid + agmatine
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bleomycin B2 + H2O
bleomycinic acid + agmatine
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bleomycin B2 + H2O
bleomycinic acid + agmatine
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specific with respect to the alkyl-chain length of the substrate
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1,10-phenanthroline
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84% inhibition at 50 mM
benzoyl-L-Arg-ethylester
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Ki: 3.6 mM
benzoyl-L-Arg-NH2
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Ki: 5 mM
Co2+
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74% inhibition at 10 mM
Ni2+
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85% inhibition at 10 mM
p-chloromercuribenzoic acid
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77% inhibition at 0.1 mM, restored by addition of cysteine or 2-mercaptoethanol
Zn2+
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95% inhibition at 10 mM
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0.22
benzoyl agmatine
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3.6
benzoyl-L-Arg-ethylester
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brenda
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brenda
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Takahashi, Y.; Shirai, T.; Ishii, S.
Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides
J. Biochem.
77
823-830
1975
Fusarium anguioides
brenda
Umezawa, H.; Takahashi, Y.; Fujii, A.; Saino, T.; Shirai, T.; Takita, T.
Preparation of bleomycinic acid: Hydrolysis of bleomycin B2 by a Fusarium acylagmatine amidohydrolase
J. Antibiot.
26
117-119
1973
Fusarium anguioides
brenda
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3.5.1.40 acylagmatine amidase
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