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Information on EC 3.4.22.7 - asclepain
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EC Tree
3.4.22.7 asclepainSpecify your search results
Word Map
- 3.4.22.7
- latex
- asclepias
-
milkweed
- casein
- asclepiadaceae
- speciosa
- fruticosa
- torr
- curassavica
- proteinases
- syriaca
- hemoglobin
-
pflna
-
petiole
- clot
- food industry
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
asclepain, asclepain f, papain-like endopeptidase, asclepain ci, asclepain cii, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
asclepain cI
asclepain f
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Similar to that of papain
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
endopeptidase
-
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CAS REGISTRY NUMBER
COMMENTARY
37288-80-5
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-pyroglutamyl-L-phenylalanyl-L-leucine 4-nitroanilide + H2O
L-pyroglutamyl-L-phenylalanyl-L-leucine + 4-nitroaniline
very low activity
-
-
?
L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide + H2O
?
-
-
-
-
?
L-pyroglutamyl-Phe-Leu-p-nitroanilide + H2O
L-pyroglutamyl-Phe-Leu + p-nitroaniline
-
-
-
-
?
N-alpha-benzyloxycarbonyl-Gly-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-Gly + 4-nitrophenol
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Ala-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Ala + 4-nitrophenol
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Asn-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Asn + 4-nitrophenol
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Asp-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Asp + 4-nitrophenol
asclepain cII has highest endoesterolytic activity using N-alpha-benzyloxycarbonyl-amino acid p-nitrophenyl esters with L-Asp as amino acid
-
-
?
N-alpha-benzyloxycarbonyl-L-Gln-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Gln + 4-nitrophenol
-
-
-
?
N-alpha-benzyloxycarbonyl-L-Tyr-4-nitrophenyl ester + H2O
N-alpha-benzyloxycarbonyl-L-Tyr + 4-nitrophenol
-
-
-
?
N-carbobenzoxy-Gly p-nitrophenyl ester + H2O
N-carbobenzoxy-Gly + p-nitrophenol
-
-
-
-
?
N-carbobenzoxy-L-Ala 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Ala + 4-nitrophenol
-
47% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-L-Gln 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Gln + 4-nitrophenol
-
-
-
-
?
N-carbobenzoxy-L-Ile 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Ile + 4-nitrophenol
-
59% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Lys + 4-nitrophenol
-
78% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-L-Tyr 4-nitrophenyl ester + H2O
N-carbobenzoxy-L-Tyr + 4-nitrophenol
-
51% of the activity with N-carbobenzoxy-L-Gln 4-nitrophenyl ester
-
-
?
N-carbobenzoxy-Leu p-nitrophenyl ester + H2O
N-carbobenzoxy-Leu + p-nitrophenol
-
-
-
-
?
N-carbobenzoxy-Lys p-nitrophenyl ester + H2O
N-carbobenzoxy-Lys + 4-nitrophenol
-
-
-
-
?
N-carbobenzoxy-Tyr p-nitrophenyl ester + H2O
N-carbobenzoxy-Tyr + p-nitrophenol
-
-
-
-
?
N-methanesulfonylglycine 3-bromophenyl ester + H2O
N-methanesulfonylglycine + 3-bromophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-chlorophenyl ester + H2O
N-methanesulfonylglycine + 3-chlorophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-fluorophenyl ester + H2O
N-methanesulfonylglycine + 3-fluorophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-methylphenyl ester + H2O
N-methanesulfonylglycine + 3-methylphenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-nitrophenyl ester + H2O
N-methanesulfonylglycine 3-nitrophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 3-tert-butylphenyl ester + H2O
N-methanesulfonylglycine + 3-tert-butylphenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-bromophenyl ester + H2O
N-methanesulfonylglycine + 4-bromophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-chlorophenyl ester + H2O
N-methanesulfonylglycine + 4-chlorophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-fluorophenyl ester + H2O
N-methanesulfonylglycine + 4-fluorophenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-methylphenyl ester + H2O
N-methanesulfonylglycine + 4-methylphenol
Asclepias sp.
-
-
-
-
?
N-methanesulfonylglycine 4-nitrophenyl ester + H2O
N-methanesulfonylglycine + 4-nitrophenol
Asclepias sp.
-
-
-
-
?
Nalpha-carbobenzoxy-Gly-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
-
about 25% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester
-
-
?
Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-L-Ala + 4-nitrophenol
-
100% activity
-
-
?
Nalpha-carbobenzoxy-L-Gln-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-L-Gln + 4-nitrophenol
-
about 85% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester
-
-
?
Nalpha-carbobenzoxy-L-Tyr-4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-L-Tyr + 4-nitrophenol
-
about 20% activity compared to Nalpha-carbobenzoxy-L-Ala-4-nitrophenyl ester
-
-
?
Nalpha-CBZ-L-Gln-4-nitrophenyl ester + H2O
Nalpha-CBZ-L-Gln + 4-nitrophenol
when assayed on N-alpha-CBZ-amino acid-4-nitrophenyl esters, the enzyme exhibits higher preference for the glutamine derivative
-
-
?
pGlu-Phe-Leu 4-nitroanilide + H2O
pGlu-Phe-Leu + 4-nitroaniline
-
-
-
?
pGlu-Phe-Leu-p-nitroanilide + H2O
pGlu-Phe-Leu + p-nitroaniline
-
-
-
?
Phe-Val-Asn-Ala-His-Leu-CysA-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + H2O
Phe-Val-Asn + Ser-His-Leu-Val-Glu-Ala-Leu + Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + Gly-Phe-Phe-Tyr-Thr
-
-
14% Phe-Val-Asn + 22% Ser-His-Leu-Val-Glu-Ala-Leu + 15% Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-CysA-Gly-Glu + 10% Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 10% Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala + 13% Gly-Phe-Phe-Tyr-Thr
?
soybean protein + H2O
?
the enzyme is able to selectively hydrolyze soybean proteins at pH 10, employing an enzyme/substrate ratio of 0.2% (w/w). The enzymatic hydrolysis allows a strong increase in the solubility, water and oil holding capacity
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
N-Benzoyl-L-Arg + ethanol
-
-
-
-
?
oxidized insulin B chain + H2O
?
12 cut-off points
-
-
?
additional information
?
-
-
asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview
-
-
?
additional information
?
-
asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview
-
-
?
additional information
?
-
asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview
-
-
?
additional information
?
-
-
asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview
-
-
?
additional information
?
-
asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview
-
-
?
additional information
?
-
asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview
-
-
?
additional information
?
-
-
the peptide PFLNA is a poor substrate for asclepain cII
-
-
?
additional information
?
-
the peptide PFLNA is a poor substrate for asclepain cII
-
-
?
additional information
?
-
the peptide PFLNA is a poor substrate for asclepain cII
-
-
?
additional information
?
-
-
no activity towards N-alpha-benzyloxycarbonyl-L-Val-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Leu-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Ile-4-nitrophenyl ester, and N-alpha-benzyloxycarbonyl-L-Lys-4-nitrophenyl ester
-
-
?
additional information
?
-
no activity towards N-alpha-benzyloxycarbonyl-L-Val-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Leu-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Ile-4-nitrophenyl ester, and N-alpha-benzyloxycarbonyl-L-Lys-4-nitrophenyl ester
-
-
?
additional information
?
-
no activity towards N-alpha-benzyloxycarbonyl-L-Val-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Leu-4-nitrophenyl ester, N-alpha-benzyloxycarbonyl-L-Ile-4-nitrophenyl ester, and N-alpha-benzyloxycarbonyl-L-Lys-4-nitrophenyl ester
-
-
?
additional information
?
-
-
ascpelain f shows low milk clotting activity
-
-
?
additional information
?
-
-
no activity towards Nalpha-carbobenzoxy-L-Leu-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Asn-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Trp-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Val-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Asp-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-lys-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Phe-4-nitrophenyl ester, Nalpha-carbobenzoxy-L-Pro-4-nitrophenyl ester, and Nalpha-carbobenzoxy-L-Ile-4-nitrophenyl ester
-
-
?
additional information
?
-
the cut specificity is governed by the presence of hydrophobic residues (F, L, V) in the P2 position
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview
-
-
?
additional information
?
-
asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview
-
-
?
additional information
?
-
asclepain cI is the main active enzyme, but shows lower specific activity than asclepain cII, which is the minor proteolytic component in the latex, overview
-
-
?
additional information
?
-
-
asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview
-
-
?
additional information
?
-
asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview
-
-
?
additional information
?
-
asclepain cII is the minor proteolytic component in the latex, but shows higher specific activity than asclepain cI, the main active enzyme, overview
-
-
?
additional information
?
-
-
ascpelain f shows low milk clotting activity
-
-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2S,3S)-3-(N-[(S)-1-[N-(4-guanidinobutyl)carbamoyl]3-methylbutyl]carbamoyl)oxirane-2-carboxylic acid
i.e. E-64, complete immediate and irreversible inhibition at 10 mM after 30 min incubation
phenylmethylsulfonyl fluoride
incubation with 100 mM phenylmethylsulfonyl fluoride for 30 min decreases activity to 63% of the initial value, but inhibition is partially reverted when 12 mM cysteine is added to the reaction mixture
additional information
-
1,10-phenantroline and pepstatine do not affect the activity of asclepain cII; activity of asclepain cII is inhibited by cysteine proteases inhibitors like E-64, but not by any other protease inhibitors such as 1,10-phenantroline, phenylmethanesulfonyl fluoride, and pepstatine
-
additional information
1,10-phenantroline and pepstatine do not affect the activity of asclepain cII; activity of asclepain cII is inhibited by cysteine proteases inhibitors like E-64, but not by any other protease inhibitors such as 1,10-phenantroline, phenylmethanesulfonyl fluoride, and pepstatine
-
additional information
1,10-phenantroline and pepstatine do not affect the activity of asclepain cII; activity of asclepain cII is inhibited by cysteine proteases inhibitors like E-64, but not by any other protease inhibitors such as 1,10-phenantroline, phenylmethanesulfonyl fluoride, and pepstatine
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.1634
N-alpha-benzyloxycarbonyl-L-Gln-4-nitrophenyl ester
at 37°C, in 0.1 M Tris-HCl buffer (pH 8.0) containing 2 mM EDTA and 25 mM cysteine
additional information
additional information
Asclepias sp.
-
Km-values for hydrolysis of aryl mesyl glycinates
-
0.73
L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide
-
pH 8.5, 42°C, Lineweaver-Burk equation
0.82
L-pyroglutamyl-L-phenylalanyl-L-leucine-4-nitroanilide
-
pH 8.5, 42°C, Eddie-Hofstee equation
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
121.5
N-alpha-benzyloxycarbonyl-L-Gln-4-nitrophenyl ester
at 37°C, in 0.1 M Tris-HCl buffer (pH 8.0) containing 2 mM EDTA and 25 mM cysteine
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
740000
N-alpha-benzyloxycarbonyl-L-Gln-4-nitrophenyl ester
at 37°C, in 0.1 M Tris-HCl buffer (pH 8.0) containing 2 mM EDTA and 25 mM cysteine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
the enzyme has a specific activity of 1.0586 units/mg in crude extract and of 12.836 units/mg after 12.1 fold purification, using casein as substrate, one arbitrary enzyme unit is defined as the amount of enzyme that produces an increase of one absorbance unit per minute
additional information
the enzyme has a specific activity of 1.0586 units/mg in crude extract and of 12.836 units/mg after 12.1 fold purification, using casein as substrate, one arbitrary enzyme unit is defined as the amount of enzyme that produces an increase of one absorbance unit per minute
additional information
the enzyme has a specific activity of 1.0586 units/mg in crude extract and of 12.836 units/mg after 12.1 fold purification, using casein as substrate, one arbitrary enzyme unit is defined as the amount of enzyme that produces an increase of one absorbance unit per minute
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 7
substrate: pGlu-Phe-Leu 4-nitroanilide
8.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 9
-
pH 5.0: about 70% of maximal activity, pH 9.0: about 30% of maximal activity, hydrolysis of N-benzoyl-L-Arg ethyl ester, asclepain Ag7
9.4 - 10.2
asclepain cII shows maximum activity within the range of pH 9.4 and 10.2 with casein as substrate
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
9.3
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Asclepias sp.
asclepain A1, A2, A3, A4, A5, and asclepain B1, B2, B3, B4 and B5
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
asclepain cII present in the latex of Asclepias curassavica is found to accelerate the blood coagulation process. Results reveal that asclepain cII present in the latex is responsible for its thrombin like activity
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). B5BLP0_9GENT
340
0
38582
TrEMBL
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE