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EC unknown
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Synonyms pernilase, more
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benzoyl-Arg-amido-4-methyl-coumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
Boc-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
Boc-Leu-Gly-Arg-amido-4-methyl-coumarin + H2O
Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin
azocasein + H2O
?
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Substrates: - Products: -
?
azocasein + H2O
?
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Substrates: - Products: -
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benzoyl-Arg-amido-4-methyl-coumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
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Substrates: about 45% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Products: -
?
benzoyl-Arg-amido-4-methyl-coumarin + H2O
benzoyl-Arg + 7-amino-4-methylcoumarin
-
Substrates: about 45% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Products: -
?
Boc-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
Substrates: about 45% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Products: -
?
Boc-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
Substrates: about 45% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Products: -
?
Boc-Leu-Gly-Arg-amido-4-methyl-coumarin + H2O
Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
-
Substrates: - Products: -
?
Boc-Leu-Gly-Arg-amido-4-methyl-coumarin + H2O
Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
-
Substrates: - Products: -
?
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
Substrates: about 20% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Products: -
?
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
Boc-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
Substrates: about 20% of the activity with Boc-Leu-Gly-Arg-amido-4-methyl-coumarin Products: -
?
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1,10-phenanthroline
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1 mM, 34% inhibition
diisopropyl fluorophosphate
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1 mM, 88% inhibition
EDTA
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1 mM, 30% inhibition
EGTA
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1 mM, 40% inhibition
PMSF
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1 mM, 87% inhibition
Soybean trypsin inhibitor
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1 mg/ml 40% inhibition
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80 - 110
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80°C: about 65% of maximal activity, 110°C: about 75% of maximal activity
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brenda
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brenda
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brenda
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brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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monomer
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1 * 50000, SDS-PAGE
monomer
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1 * 50000, SDS-PAGE
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2-mercaptoethanol
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1%, resistant to
2-mercaptoethanol
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1%, resistant to
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dithiothreitol
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10%, resistant to
dithiothreitol
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10%, resistant to
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guanidine-HCl
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4 M, complete inactivation
guanidine-HCl
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4 M, complete inactivation
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SDS
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2%, 97% inactivation
SDS
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2%, 97% inactivation
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urea
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8 M, 88% inactivation
urea
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8 M, 88% inactivation
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highly resistant to the reducing agents dithiothreitol and 2-mercaptoethanol
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727450
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Chavez Croocker, P.; Sako, Y.; Uchida, A.
Purification and characterization of an intracellular heat-stable proteinase (pernilase) from the marine hyperthermophilic archaeon Aeropyrum pernix K1.
Extremophiles
3
3-9
1999
Aeropyrum pernix, Aeropyrum pernix DSM 11879
brenda
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