Reference on EC 3.4.21.97 - assemblin
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Kan, C.C.
Cytomegalovirus assemblin
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
468-470
1998
Human betaherpesvirus 5, simian cytomegalovirus
-
Darke, P.L.
Herpesvirus assemblin
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
470-472
1998
Bovine alphaherpesvirus 1, Equid alphaherpesvirus 1, equine herpesvirus type 2, Herpes simplex virus, Human alphaherpesvirus 1, Human alphaherpesvirus 2, Saimiriine gammaherpesvirus 2, Suid alphaherpesvirus 1
-
Jupp, R.; Mills, J.; Tigue, N.J.; Kay, J.
Human herpesvirus type 6 assemblin
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
472-474
1998
Human betaherpesvirus 6
-
Jupp, R.; Ritchie, A.; Robinson, M.; Broadhurst, A.; Mills, J.
Epstein-Barr virus assemblin
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
474-476
1998
Human gammaherpesvirus 4
-
McMillan, D.M.; Jupp, R.; Mills, J.; Kay, J.
Varicella-zoster virus assemblin
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
476-478
1998
Human alphaherpesvirus 3
-
Chen, P.; Tsuge, H.; Almassy, R.J.; Gribskov, C.L.; Katoh, S.; Vanderpool, D.L.; Margosiak, S.A.; Pinko, C.; Matthews, D.A.; Kan, C.C.
Structure of the human cytomegalovirus protease catalytic domain reveals a novel serine protease fold and catalytic triad
Cell
86
835-843
1996
Human betaherpesvirus 5
Holwerda, B.
Activity in monomers of human cytomegalovirus protease
Biochem. Biophys. Res. Commun.
259
370-373
1999
Human betaherpesvirus 5
Yoakim, C.; Ogilvie, W.W.; Cameron, D.R.; Chabot, C.; Guse, I.; Hache, B.; Naud, J.; OMeara, J.A.; Palnte, R.; Deziel, R.
beta-Lactam derivatives as inhibitors of human cytomegalovirus protease
J. Med. Chem.
41
2882-2891
1998
Human betaherpesvirus 5
Tomasselli, A.G.; Paddock, D.J.; Curry, K.A.; Garlick, R.L.; Leone, J.W.; Lull, J.M.; Mutchler, V.T.; Baker, C.A.; Cavey, G.S.; Methews, W.R.; Shelly, J.A.; Finzel, B.C.; Baldwin, E.T.; Wells, P.A.; Tomich, C.S.C.
Recombinant human cytomegalovirus protease with a C-terminal (His)6 extension: purification, autocatalytic release of the mature enzyme, and biochemical characterization
Protein Expr. Purif.
14
343-352
1996
Human betaherpesvirus 5
McKercher, G.; Bonneau, P.R.; Lagace, L.; Thibeault, D.; Massaiol, M.J.; Krogsrud, R.; Lawetz, C.; McDonald, P.C.; Cordingley, M.G.
Improved purification protocol of the HSV-1 protease catalytic domain, using immunoaffinity
Biochem. Cell Biol.
75
795-801
1997
Human alphaherpesvirus 1
Gibson, W.; Hall, M.R.T.
Assemblin, an essential herpesvirus proteinase
Drug Des. Discov.
15
39-47
1997
Human betaherpesvirus 5
Hall, M.R.T.; Gibson, W.
Independently cloned halves of cytomegalovirus assemblin An and Ac can restore proteolytic activity to assemblin mutants by intermolecular complementation
J. Virol.
71
956-964
1997
Human betaherpesvirus 5
Bonneau, P.R.; Grand-Maitre, C.; Greenwood, D.J.; Lagac, L.; LaPlante, S.R.; Massariol., M.J.; Ogilvie, W.W.; OMeara, J.A.; Kawai, S.H.
Evidence of a conformational change in the human cytomegalovirus protease upon binding of peptidyl-activated carbonyl inhibitors
Biochemistry
36
12644-12652
1997
Human betaherpesvirus 5
Hoog, S.S.; Smith, W.W.; Qiu, X.; Janson, C.A.; Hellmig, B.; McQuerney, M.S.; ODonnell, K.; OShannessy, D.; DiLella, A.G.; Abdel-Meguid, S.S.
Active site cavity of herpesvirus protease revealed by the crystal structure of Herpes simplex virus protease/inhibitor complex
Biochemistry
36
14023-14029
1997
Herpes simplex virus, Human alphaherpesvirus 1
Margosiak, S.A.; Vanderpool, D.L.; Sisson, W.; Pinko, C.; Kan, C.C.
Dimerization of the human cytomegaolvirus protease: kinetic and biochemical characterization of the catalytic homodimer
Biochemistry
35
5300-5307
1996
Human betaherpesvirus 5
Shieh, H.S.; Kurumbail, R.G.; Stevens, A.M.; Stegeman, R.A.; Sturman, E.J.; Pak, J.Y.; Wittwer, A.J.; Palmier, M.O.; Wiegand, R.C.; Holwerda, B.C.; Stallings, W.C.
Three-dimensional structure of human cytomegalovirus protease
Nature
383
279-282
1996
Human betaherpesvirus 5
Tong, L.; Qian, C.; Massariol, M.J.; Bonneau, P.R.; Cordingley, M.G.; Lagace, L.
A new serine-protease fold revealed by the crystal strucutre of human cytomegalovirus protease
Nature
383
272-275
1996
Human betaherpesvirus 5
Snyder, S.W.; Edalji, R.P.; Lindh, F.G.; Walter, K.A.; Solomon, L.; Pratt, S.; Steffy, K.; Holzman, T.F.
Initial characterization of autoprocessing and active-center mutants of CMV proteinase
J. Protein Chem.
15
763-774
1996
Human betaherpesvirus 5
LaFemina, R.L.; Bakshl, K.; Long, W.J.; Pramanik, B.; Veloski, C.A.; Wolanski, B.S.; Marcy, A.L.; Hazuda, D.J.
Characterization of a soluble stable human cytomegalovirus protease and inhibition by M-site peptide mimics
J. Virol.
70
4819-4824
1996
Human betaherpesvirus 5
Darke, P.L.; Cole, J.L.; Waxman, L.; Hall, D.L.; Sardana, K.; Kuo, L.C.
Active human cytomegalovirus protease is a dimer
J. Biol. Chem.
271
7445-7449
1996
Human betaherpesvirus 5
Darke, P.L.; Chen, E.; Hall, D.L.; Sardanna, M.K.; Veloski, C.A.; LaFemina, R.L.; Shafer, J.A.; Kuo, L.C.
Purification of active Herpes simplex virus-1 protease expressed in Escherichia coli
J. Biol. Chem.
269
18708-18711
1994
Human alphaherpesvirus 1
Hall, M.R.T.; Gibson, W.
Cytomegalovirus assemblin: the amino and carboxy domains of the proteinase form active enzyme when separately cloned and coexpressed in eukaryotic cells
J. Virol.
70
5395-5404
1996
Human betaherpesvirus 5
Burck, P.J.; Berg, D.H.; Luk, T.P.; Sassmannshausen, L.M.; Wakulchik, M.; Smith, D.P.; Hsiung, H.M.; Becker, G.W.; Gibson, W.; Villarreal, E.C.
Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites
J. Virol.
68
2937-2946
1994
Human betaherpesvirus 5
Smith, M.C.; Giordano, J.; Cook, J.A.; Wakulchik, M.; Villarreal, E.C.; Becker, G.W.; Bemis, K.; Labus, J.; Manetta, J.S.
Purification and kinetic characterization of human cytomegalovirus assemblin
Methods Enzymol.
244
417-423
1994
Human betaherpesvirus 5
-
Tigue, N.; Matharu, P.J.; Roberts, N.A.; Mills, J.S.; Kay, J.; Rupp, R.
Cloning, expression, and characterization of the proteinase from human herpesvirus 6
J. Virol.
70
4136-4141
1996
Human betaherpesvirus 5, Human betaherpesvirus 6
Donaghy, G.; Jupp, R.
Characterization of the Epstein-Barr virus proteinase and comparison with the human cytomegalovirus proteinase
J. Virol.
69
1265-1270
1995
Human gammaherpesvirus 4, Human betaherpesvirus 5
McMillan, D.J.; Kay, J.; Mills, J.S.
Characterization ot the proteinase specified by vircella-zoster virus gene 33
J. Gen. Virol.
78
2153-2157
1997
Human alphaherpesvirus 3
Qian, C.; Lagace, L.; Massariol, M.J.; Chabot, C.; Yoakim, C.; Deziel, R.; Tong, L.
A rational approach towards successful crystallization and crystal treatment of human cytomegalovirus protease and its inhibitor complex
Acta Crystallogr. Sect. D
56
175-180
2000
Human betaherpesvirus 5
Khayat, R.; Batra, R.; Massariol, M.J.; Lagace, L.; Tong, L.
Investigating the role of histidine 157 in the catalytic activity of human cytomegalovirus protease
Biochemistry
40
6344-6351
2001
Human betaherpesvirus 5
Khayat, R.; Batra, R.; Qian, C.; Halmos, T.; Bailey, M.; Tong, L.
Structural and biochemical studies of inhibitor binding to human cytomegalovirus protease
Biochemistry
42
885-891
2003
Human betaherpesvirus 5
Khayat, R.; Batra, R.; Bebernitz, G.A.; Olson, M.W.; Tong, L.
Characterization of the monomer-dimer equilibrium of human cytomegalovirus protease by kinetic methods
Biochemistry
43
316-322
2004
Human betaherpesvirus 5
Borthwick, A.D.; Angier, S.J.; Crame, A.J.; Exall, A.M.; Haley, T.M.; Hart, G.J.; Mason, A.M.; Pennell, A.M.; Weingarten, G.G.
Design and synthesis of pyrrolidine-5,5-trans-lactams (5-oxo-hexahydro-pyrrolo[3,2-b]pyrroles) as novel mechanism-based inhibitors of human cytomegalovirus protease. 1. The alpha-methyl-trans-lactam template
J. Med. Chem.
43
4452-4464
2000
Human betaherpesvirus 5
Borthwick, A.D.; Crame, A.J.; Ertl, P.F.; Exall, A.M.; Haley, T.M.; Hart, G.J.; Mason, A.M.; Pennell, A.M.; Singh, O.M.; Weingarten, G.G.; Woolven, J.M.
Design and synthesis of pyrrolidine-5,5-trans-lactams (5-oxohexahydropyrrolo[3,2-b]pyrroles) as novel mechanism-based inhibitors of human cytomegalovirus protease. 2. Potency and chirality
J. Med. Chem.
45
1-18
2002
Human betaherpesvirus 5
Borthwick, A.D.; Davies, D.E.; Ertl, P.F.; Exall, A.M.; Haley, T.M.; Hart, G.J.; Jackson, D.L.; Parry, N.R.; Patikis, A.; Trivedi, N.; Weingarten, G.G.; Woolven, J.M.
Design and synthesis of pyrrolidine-5,5'-trans-lactams (5-oxo-hexahydropyrrolo[3,2-b]pyrroles) as novel mechanism-based inhibitors of human cytomegalovirus protease. 4. Antiviral activity and plasma stability
J. Med. Chem.
46
4428-4449
2003
Human betaherpesvirus 5
Chan, C.K.; Brignole, E.J.; Gibson, W.
Cytomegalovirus assemblin (pUL80a): cleavage at internal site not essential for virus growth; proteinase absent from virions
J. Virol.
76
8667-8674
2002
Human betaherpesvirus 5
Batra, R.; Khayat, R.; Tong, L.
Molecular mechanism for dimerization to regulate the catalytic activity of human cytomegalovirus protease
Nat. Struct. Biol.
8
810-817
2001
Human betaherpesvirus 5
de Oliveira, C.A.; Guimaraes, C.R.; Barreiro, G.; de Alencastro, R.B.
Investigation of the induced-fit mechanism and catalytic activity of the human cytomegalovirus protease homodimer via molecular dynamics simulations
Proteins Struct. Funct. Genet.
52
483-491
2003
Human betaherpesvirus 5
geitmann, M.; Danielson, E.H.
Studies of substrate-induced conformational changes in human cytomegalovirus protease using optical biosensor technology
Anal. Biochem.
332
203-214
2004
Human betaherpesvirus 5
McCartney, S.A.; Brignole, E.J.; Kolegraff, K.N.; Loveland, A.N.; Ussin, L.M.; Gibson, W.
Chemical rescue of I-site cleavage in living cells and in vitro discriminates between the cytomegalovirus protease, assemblin, and its precursor, pUL80a
J. Biol. Chem.
280
33206-33212
2005
simian cytomegalovirus
Loveland, A.N.; Chan, C.K.; Brignole, E.J.; Gibson, W.
Cleavage of human cytomegalovirus protease pUL80a at internal and cryptic sites is not essential but enhances infectivity
J. Virol.
79
12961-12968
2005
Human betaherpesvirus 5
Cottier, V.; Barberis, A.; Luethi, U.
Novel yeast cell-based assay to screen for inhibitors of human cytomegalovirus protease in a high-throughput format
Antimicrob. Agents Chemother.
50
565-571
2006
Human betaherpesvirus 5
Fernandes de Oliveira, C.A.; Werneck Guimaraes, C.R.; Barreiro, G.; Bicca de Alencastro, R.
Human cytomegalovirus protease: Why is the dimer required for catalytic activity?
J. Chem. Theory Comput.
3
278-288
2007
Human betaherpesvirus 5
Brignole, E.J.; Gibson, W.
Enzymatic activities of human cytomegalovirus maturational protease assemblin and its precursor (pPR, pUL80a) are comparable: [corrected] maximal activity of pPR requires self-interaction through its scaffolding domain
J. Virol.
81
4091-4103
2007
Human betaherpesvirus 5
Harper, J.D.; Thuet, J.; Lechtreck, K.F.; Hardham, A.R.
Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates
Protoplasma
236
97-101
2009
Paramecium tetraurelia, Perkinsus marinus, Phaeodactylum tricornutum, Phytophthora infestans, Phytophthora nicotianae, Phytophthora ramorum, Phytophthora sojae, Tetrahymena thermophila, Thalassiosira pseudonana
Fernandes, S.; Brignole, E.; Taori, K.; Gibson, W.
Cytomegalovirus capsid protease: Biological substrates are cleaved more efficiently by full-length enzyme (pUL80a) than by the catalytic domain (Assemblin)
J. Virol.
85
3526-3534
2011
Cytomegalovirus
Gable, J.; Lee, G.; Jaishankar, P.; Hearn, B.; Waddling, C.; Renslo, A.; Craik, C.
Broad-spectrum allosteric inhibition of herpesvirus proteases
Biochemistry
53
4648-4660
2014
Human gammaherpesvirus 4, Human alphaherpesvirus 2, Human betaherpesvirus 5, Human gammaherpesvirus 8
Sabit, H.; Dahan, A.; Sun, J.; Provoda, C.J.; Lee, K.D.; Hilfinger, J.H.; Amidon, G.L.
Cytomegalovirus protease targeted prodrug development
Mol. Pharm.
10
1417-1424
2013
Human betaherpesvirus 5
Yang, K.; Wills, E.G.; Baines, J.D.
Release of the herpes simplex virus 1 protease by self cleavage is required for proper conformation of the portal vertex
Virology
429
63-73
2012
Herpes simplex virus
Zuehlsdorf, M.; Werten, S.; Klupp, B.G.; Palm, G.J.; Mettenleiter, T.C.; Hinrichs, W.
Dimerization-induced allosteric changes of the oxyanion-hole loop activate the pseudorabies virus assemblin pUL26N, a herpesvirus serine protease
PLoS Pathog.
11
e1005045
2015
Suid alphaherpesvirus 1 (Q83417), Suid alphaherpesvirus 1, Suid alphaherpesvirus 1 Kaplan (Q83417)
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