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(benzyloxycarbonyl-L-alanyl-L-arginine)2-rhodamine-110 + 2 H2O
2 benzyloxycarbonyl-L-alanyl-L-arginine + rhodamine-110
-
specific trypsin substrate
-
-
?
2-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AAGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
2-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AEGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
2-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AFGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGR + MAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + MAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRAAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + AAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRDAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + DAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGREAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + EAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRFAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + FAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRHAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + HAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRIAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + IAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRKAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + KAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRLAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + LAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRMAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + MAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRNAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + NAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRQAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + QAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRSAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + SAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRTAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + TAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRVAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + VAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGGRYAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGGR + YAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGHR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
2-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGLR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
2-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGMR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
2-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGNR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
2-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGTR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
2-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGYR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AIGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AKGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-ALGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AMGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AQGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AVGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
4-methylumbelliferyl-4-guanidinobenzoate + H2O
4-methylumbelliferol + 4-guanidinobenzoate
-
-
-
-
?
Abz-ERFK-EDDnp + H2O
Abz-ER + FK-EDDnp
-
-
-
-
?
Abz-FRFK-EDDnp + H2O
Abz-FR + FK-EDDnp
-
-
-
-
?
Abz-GRFK-EDDnp + H2O
Abz-GR + FK-EDDnp
-
-
-
-
?
Abz-HRFK-EDDnp + H2O
Abz-HR + FK-EDDnp
-
-
-
-
?
Abz-KRFK-EDDnp + H2O
Abz-KR + FK-EDDnp
-
-
-
-
?
Abz-LRFK-EDDnp + H2O
Abz-LR + FK-EDDnp
-
-
-
-
?
Abz-NRFK-EDDnp + H2O
Abz-NR + FK-EDDnp
-
-
-
-
?
Abz-QRFK-EDDnp + H2O
Abz-QR + FK-EDDnp
-
-
-
-
?
Abz-RDFK-EDDnp + H2O
Abz-R + DFK-EDDnp
-
-
-
-
?
Abz-REFK-EDDnp + H2O
Abz-R + EFK-EDDnp
-
-
-
-
?
Abz-RGFK-EDDnp + H2O
Abz-R + GFK-EDDnp
-
-
-
-
?
Abz-RHFK-EDDnp + H2O
Abz-R + HFK-EDDnp
-
-
-
-
?
Abz-RNFK-EDDnp + H2O
Abz-R + NFK-EDDnp
-
-
-
-
?
Abz-RPFK-EDDnp + H2O
Abz-R + PFK-EDDnp
-
-
-
-
?
Abz-RQFK-EDDnp + H2O
Abz-R + QFK-EDDnp
-
-
-
-
?
Abz-RRFK-EDDnp + H2O
Abz-R + RFK-EDDnp
-
-
-
-
?
Abz-RSFK-EDDnp + H2O
Abz-R + SFK-EDDnp
-
-
-
-
?
Abz-RTFK-EDDnp + H2O
Abz-R + TFK-EDDnp
-
-
-
-
?
Abz-RVFK-EDDnp + H2O
Abz-R + VFK-EDDnp
-
-
-
-
?
Abz-RYFK-EDDnp + H2O
Abz-R + YFK-EDDnp
-
-
-
-
?
Abz-VRFK-EDDnp + H2O
Abz-VR + FK-EDDnp
-
-
-
-
?
Abz-WRFK-EDDnp + H2O
Abz-WR + FK-EDDnp
-
-
-
-
?
Abz-YRFK-EDDnp + H2O
Abz-YR + FK-EDDnp
-
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg(NO2)-4-nitroanilide + H2O
?
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg-4-nitroanilide + H2O
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg + 4-nitroaniline
-
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Har-4-nitroanilide + H2O
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Har + 4-nitroaniline
-
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Lys-4-nitroanilide + H2O
?
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-N-omega-hydroxy-L-arginine-4-nitroanilide + H2O
?
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-CN)-4-nitroanilide + H2O
?
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-NH2)-4-nitroanilide + H2O
?
-
-
-
?
acetyl-L-Phe-L-Ala-L-Thr-(4-guanidino)-L-Phe-NH-(3-carbamoyl-4-nitrophenol) + H2O
acetyl-L-Phe-L-Ala-L-Thr-(4-guanidino)-L-Phe + 5-amino-2-nitrobenzamide
-
-
-
-
?
AGPYAHSS + H2O
?
-
trypsin N143H/E151H hydrolyzes the peptide exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency
-
-
?
alpha-amylase + H2O
?
-
-
-
?
alpha-casein + H2O
?
-
-
-
-
?
alpha-N-p-tosyl-L-arginine methyl ester + H2O
alpha-N-p-tosyl-L-arginine + methanol
-
-
-
-
?
ANVIPFKVHFRAAFC + H2O
ANVIPFK + VHFR + AAFC
-
-
-
-
?
apomyoglobin + H2O
?
-
-
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
-
-
?
benzoyl-Arg 4-nitroanilide + H2O
benzoyl-Arg + 4-nitroaniline
-
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-Arg + ethanol
-
-
-
?
benzoyl-Arg ethyl ester + leucinamide
benzoyl-Arg leucinamide + ethanol
-
-
-
?
benzoyl-Arg-4-nitroanilide + H2O
benzoyl-Arg + 4-nitroaniline
-
-
?
benzoyl-Arg-p-nitroanilide + H2O
benzoyl-Arg + p-nitroaniline
benzoyl-DL-Arg-4-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
benzoyl-DL-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-DL-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide + H2O
benzoyl-DL-Arg + p-nitroaniline
-
-
-
-
?
benzoyl-DL-arginine-p-nitroanilide + H2O
benzoyl-DL-arginine + p-nitroaniline
-
-
-
-
?
benzoyl-L-Arg amide + H2O
?
-
beta-trypsin is about 40% more active than alpha trypsin
-
-
?
benzoyl-L-Arg-4-nitroanilide + H2O
benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
benzoyl-L-arginine-p-nitroanilide + H2O
benzoyl-L-arginine + p-nitroaniline
benzyloxycarbonyl-Arg-4-nitroanilide + H2O
benzyloxycarbonyl-Arg + 4-nitrolaniline
-
-
?
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
?
-
-
?
benzyloxycarbonyl-Arg-thiobenzyl ester + H2O
benzyloxycarbonyl-Arg + phenylmethanethiol
-
-
-
-
?
benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
94.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Glu-Arg-Arg + 7-amino-4-methylcoumarin
75.2% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin
14.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide + H2O
benzyloxycarbonyl-Gly-Pro-Arg + p-nitroaniline
-
-
-
-
?
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-L-glutamyl-L-lysyl-L-lysine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-glutamyl-L-lysyl-L-lysine + 7-amino-4-methylcoumarin
-
low activity
-
-
?
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-L-Lys benzoyl ester + H2O
benzyloxycarbonyl-L-Lys + benzoate
-
-
-
-
?
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg-Arg + 7-amino-4-methylcoumarin
51.4% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
44.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
Benzyloxycarbonyl-Lys methyl ester + H2O
Benzyloxycarbonyl-Lys + methanol
-
-
-
-
?
benzyloxycarbonyl-Lys p-nitrophenyl ester + H2O
benzyloxycarbonyl-Lys + p-nitrophenol
-
-
-
-
?
benzyloxycarbonyl-Lys-thiobenzyl ester + H2O
benzyloxycarbonyl-Lys + phenylmethanethiol
benzyloxycarbonyl-Phe-Arg-4-nitroanilide + H2O
?
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
?
-
-
-
?
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
-
-
?
benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
27.1% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
33.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonylglycyl-L-prolyl-L-arginine + 7-amino-4-methylcoumarin
beta-crystallin + H2O
?
-
-
-
-
?
beta-lactoglobulin + H2O
?
-
-
-
-
?
bovine heart cytochrome c + H2O
?
-
-
-
-
?
bovine M-proinsulin + H2O
?
-
insulin containing methionine at the N-terminus
cleaved at the K59R60-G61 bond of the C/A junction, but at the B/C junction cleavage occurs at the R31R32-E33 as well as the R31-R32E33 bond. Cleavage at the B/C junction is preferred (65%) over that at the C/A junction (35%)
-
?
Bovine serum albumin + H2O
?
bovine serum albumin + H2O
LVVSTQTALA + ?
-
-
-
-
?
bovine skin DQ-collagen type I + H2O
?
calmodulin + H2O
?
-
-
-
-
?
carbobenzoxy-L-Arg-7-amido-4-methylcoumarin + H2O
carbobenzoxy-L-Arg + 7-amino-4-methylcoumarin
carbonic anhydrase + H2O
?
-
-
-
?
Cbz-L-Arg-7-amido-4-methylcoumarin + H2O
Cbz-L-Arg + 7-amino-4-methylcoumarin
Collagen + H2O
?
-
trypsin has little activity against collagen
-
-
?
Cry1Ab protoxin + H2O
Cry1Ab toxin
-
-
-
-
?
D-Phe-Pip-Arg 4-nitroanilide + H2O
?
-
-
?
D-Phe-Pip-Arg 4-nitroanilide + H2O
D-Phe-Pip-Arg + 4-nitroaniline
-
-
-
-
?
D-Val-Leu-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
D-Val-Leu-Lys-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
D-Val-Leu-Lys-thiobenzyl ester + H2O
?
-
-
-
-
?
DrmSP8-36 + H2O
?
-
trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph
-
-
?
enhanced green fluorescent protein-T1 + H2O
?
-
enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa
-
-
?
enhanced green fluorescent protein-T1nb + H2O
?
-
-
-
-
?
enhanced green fluorescent protein-T2 + H2O
?
-
enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa
-
-
?
epithelial sodium channel + H2O
?
factor Xa + H2O
?
-
-
-
?
Fibronectin + H2O
?
-
-
-
-
?
FITC-casein + H2O
?
-
fluorescence-labeled casein
-
-
?
gamma-crystallin + H2O
?
-
-
-
-
?
GDDSTPSILPAPRGYPGQV + H2O
GDDSTPSILPAPR + GYPGQV
-
-
-
-
?
Gelatin + H2O
?
-
-
-
-
?
GERGFFYTPKT + H2O
?
-
insulin-derived peptide
cleavage at bond K-T
-
?
GERGFFYTPKTRR + H2O
?
-
insulin-derived peptide
cleavage at bond K-T
-
?
Glu-Gly-Arg-4-nitroanilide + H2O
?
-
-
-
?
glucose oxidase + H2O
?
-
-
-
?
Gly-Pro-Arg-p-nitroanilide + H2O
Gly-Pro-Arg + p-nitroaniline
-
-
-
-
?
glycogen phosphorylase b + H2O
?
-
-
-
?
GPNSKGRSLIGRLDTPYGGC + H2O
GPNSKGR + SLIGRLDTPYGGC
-
-
-
-
?
high pathogenic hemagglutinin + H2O
?
-
-
-
-
?
human cartilage collagen type II + H2O
?
human M-proinsulin + H2O
?
-
insulin containing methionine at the N-terminus
cleavage at the R31R32-E33 and K64R65-G66 bonds, i.e. B/C and C/A junctions. Cleavage at the B/C junction is preferred (65%) over that at the C/A junction (35%)
-
?
human placenta DQ-collagen type IV + H2O
?
insulin
?
-
-
cleavage at bond K29-T30
-
?
inter-alpha-trypsin inhibitor heavy chain H4 + H2O
?
-
inter-alpha-trypsin inhibitor heavy chain H4 is also a marker of acute ischemic stroke
-
-
?
intestinal alkaline sphingomyelinase bound to mucosa + H2O
free intestinal alkaline sphingomyelinase
L-Phe-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
L-Phe-L-Pro-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Phe-L-Val-L-Ile-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
L-Phe-L-Val-L-Ile-L-Arg + 5-amino-2-nitrobenzamide
-
-
-
-
?
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
L-Phe-L-Val-L-Ile-L-Arg + 5-amino-2-nitrobenzamide
-
-
-
-
?
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide + H2O
L-Phe-L-Val-L-Ile-L-Lys + 5-amino-2-nitrobenzamide
-
-
-
-
?
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
L-Phe-L-Val-L-Pro-L-Arg + 5-amino-2-nitrobenzamide
-
-
-
-
?
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide + H2O
L-Phe-L-Val-L-Pro-L-Lys + 5-amino-2-nitrobenzamide
-
-
-
-
?
L-Tat (47-58) + H2O
?
-
trypsin significantly inactivates L-Tat (47-58)
-
-
?
low pathogenic hemagglutinin + H2O
?
-
-
-
-
?
lubricin + H2O
?
-
-
-
-
?
M-diarginyl insulin + H2O
?
-
insulin containing methionine at the N-terminus
cleavage at bond K29-T30
-
?
M-insulin
?
-
insulin containing methionine at the N-terminus
cleavage at bond K29-T30
-
?
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide + H2O
MeOSuc-Ala-Ala-Ala-Arg + 4-nitroaniline
-
-
-
-
?
MeOSuc-Ala-Ala-Arg 4-nitroanilide + H2O
MeOSuc-Ala-Ala-Arg + 4-nitroaniline
-
-
-
-
?
MeOSuc-Ala-Arg 4-nitroanilide + H2O
MeOSuc-Ala-Arg + 4-nitroaniline
-
-
-
-
?
Myoglobin + H2O
?
-
-
-
?
N-(p-tosyl)-Gly-Pro-Arg 4-nitroanilide + H2O
N-(p-tosyl)-Gly-Pro-Arg + 4-nitroaniline
-
-
-
-
?
N-acetyl-GTNRSSKGRSLIGKVDGTSHVTGKGVT-amide + H2O
SLIGKVDGTSHVTGKGVT-amide + N-acetyl-GTNRSSKGR
-
-
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
N-alpha-benzoyl-DL-arginine-4-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + 4-nitroaniline
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + p-nitroaniline
N-alpha-benzoyl-L-Arg ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
N-alpha-benzoyl-L-Arg methyl ester + H2O
N-alpha-benzoyl-L-Arg + methanol
N-alpha-benzoyl-L-Arg-2-naphthylamide + H2O
N-alpha-benzoyl-L-Arg + 2-naphthylamine
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
N-alpha-benzoyl-L-Arg-ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
N-alpha-benzoyl-L-arginine 4-nitroanilide + H2O
N-alpha-benzoyl-L-arginine + 4-nitroaniline
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-arginine + ethanol
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-L-arginine + p-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Lys-p-nitroanilide + H2O
N-alpha-benzoyl-L-Lys + p-nitroaniline
N-alpha-benzoyl-L-Phe-L-Val-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Phe-L-Val-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-p-tosyl-L-Arg methyl ester + H2O
N-alpha-p-tosyl-L-Arg + methanol
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + ?
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + methanol
N-benzoyl-DL-Arg-4-nitroanilide + H2O
N-benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-Arg-4-nitroanilide + H2O
N-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
N-benzoyl-DL-arginine-4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
?
N-benzoyl-DL-arginyl-4-nitroanilide + H2O
?
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
N-benzoyl-L-Arg-4-nitroanilide + H2O
N-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-benzoyl-L-arginine 4-nitroanilide + H2O
N-benzoyl-L-arginine + 4-nitroaniline
-
the trypsin-like proteinases from the resistant strains exhibited higher affinity for the substrate than the enzymes from the susceptible strains
-
-
?
N-benzoyl-L-arginine ethyl ester + H2O
N-benzoyl-L-arginine + ethanol
-
-
-
-
?
N-benzoyl-L-arginine-p-nitroanilide + H2O
N-benzoyl-L-arginine + p-nitroaniline
-
-
-
-
?
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine methyl ester + H2O
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + methanol
-
-
-
-
?
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide + H2O
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + 4-nitroaniline
-
-
-
-
?
N-benzyloxycarbonyl-Gly-L-Pro-L-Arg-4-nitroanilide + H2O
N-benzyloxycarbonyl-Gly-L-Pro-L-Arg + 4-nitroaniline
-
-
-
-
?
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide + H2O
N-benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline
-
-
-
-
?
N-benzyloxycarbonyl-L-lysine benzyl ester + H2O
N-benzyloxycarbonyl-L-lysine + toluene
-
-
-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide + H2O
N-carbobenzyloxy-Gly-Pro-Arg + p-nitroaniline
Nafamostat + H2O
?
-
extremely poor substrate
-
?
Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide + H2O
Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine + 4-nitroaniline
-
-
-
-
?
Nalpha-4-tosyl-L-Arg methyl ester + H2O
?
-
-
-
-
?
Nalpha-4-tosyl-L-arginine methyl ester + H2O
Nalpha-4-tosyl-L-arginine + methanol
Nalpha-benzoyl-DL-Arg 2-naphthylamide + H2O
?
-
-
-
-
?
Nalpha-Benzoyl-DL-Arg 4-nitroanilide + H2O
Nalpha-Benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-arginine + ethanol
Nalpha-Boc-D-Ala-(p-amidino)phenyl ester + D-Ala-4-nitroanilide
Nalpha-Boc-D-Ala-D-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nalpha-Boc-D-Ala-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Ala-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
-
-
-
-
?
Nalpha-Boc-D-Ala-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Ala-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nalpha-Boc-D-Leu-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Leu-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nalpha-Boc-D-Leu-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Leu-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
-
-
-
-
?
Nalpha-Boc-D-Phe-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Phe-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nalpha-Boc-D-Phe-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Phe-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
-
-
-
-
?
Nalpha-Boc-Gly-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-Gly-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nalpha-Boc-Gly-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-Gly-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
-
-
-
-
?
Nalpha-Boc-L-Ala-(p-amidino)phenyl ester + D-Ala-4-nitroanilide
Nalpha-Boc-L-Ala-D-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nalpha-Boc-L-Ala-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Ala-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nalpha-Boc-L-Ala-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Ala-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
-
-
-
-
?
Nalpha-Boc-L-Leu-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Leu-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nalpha-Boc-L-Leu-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Leu-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
-
-
-
-
?
Nalpha-Boc-L-Phe-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Phe-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nalpha-Boc-L-Phe-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Phe-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
-
-
-
-
?
Nalpha-p-tosyl-L-Arg methyl ester + H2O
?
Nalpha-p-tosyl-L-arginine methyl ester + H2O
?
NATLDPRSFLLRNPNDKYE + H2O
NATLDPR + SFLLRNPNDKYE
-
-
-
-
?
Nbeta-Boc-beta-Ala-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nbeta-Boc-beta-Ala-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
-
-
-
-
?
Nbeta-Boc-beta-Ala-(p-guanidino)phenyl ester + beta-Ala-4-nitroanilide
Nbeta-Boc-beta-Ala-beta-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
-
-
-
-
?
Nbeta-Boc-beta-Ala-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nbeta-Boc-beta-Ala-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
-
-
-
-
?
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AAGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ADGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AEGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AFGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGAR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGDR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGER + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGFR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGK + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGGRAAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRDAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GDGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGREAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GEGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRFAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GFGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGGRGDGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GDGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGEGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GEGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGFGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GFGQ-(2,4-dinitrophenyl-ethylene diamine
-
-
-
-
?
o-aminobenzoyl-AGGRGGGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GGGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGGRGHGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GHGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGIGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GIGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGKGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GKGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGLGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GLGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGMGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GMGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGNGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GNGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGQGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GQGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGSGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GSGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGTGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GTGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGVGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GVGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGYGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GYGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRHAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GHGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRIAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GIGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRKAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GKGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRLAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GLGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRMAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GMGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRNAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GNGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRQAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GQGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRSAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GSGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRTAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GTGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRVAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GVGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRYAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GYGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGHR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGIR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGKR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGLR + GAGQ-(2,4-dinitrophenyl-ethylene diamine
-
-
-
-
?
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGLR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGMR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGNR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGQR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGSR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGTR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGVR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGYR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AHGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AIGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AKGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ALGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AMGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ANGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AQGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ASGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ATGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AVGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AYGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
ovalbumin + H2O
?
-
-
-
?
p-amidinophenyl acetate + H2O
p-amidinophenol + acetate
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
-
-
-
?
p-nitrophenyl p'-guanidinobenzoate + H2O
?
-
-
-
-
?
p-nitrophenyl-p'-guanidino benzoate + H2O
p-nitrophenol + p-guanidinobenzoate
p-tosyl-L-Arg methyl ester + H2O
?
Pefachrome + H2O
?
-
-
-
?
Pefachrome tPA + H2O
?
-
-
-
?
peroxidase + H2O
?
-
-
-
?
pre-Pro-Arg-insulin + H2O
mono/di-Arg insulin + ?
-
-
-
-
?
Pro-Arg-insulin + H2O
mono/di-Arg insulin + ?
-
-
-
-
?
pro-human defensin-5 + H2O
human defensin-5 + pro-human defensin-5 propeptide
-
pro-human defensin-5 in the intestinal lumen is processed by trypsin in a complex in which chymotrypsinogen is also cleaved for activation
-
-
?
pro-insulin + H2O
human insulin-threonine-ester
-
-
-
-
?
pro-matrix metalloproteinase-2 + H2O
matrix metalloproteinase-2 + pro-peptide
-
activation by trypsin depends on various factors such as the level of exogenously added Ca2+ and Brij-35, temperature, and trypsin concentration. Activation occurs as sequential processing, initially generating an active 62 kDa species followed by successive truncation of the C-terminal domain leading to active species of 56 kDa, 52 kDa and 50kDa. Comparison with activation of pro-matrix metalloproteinase-2 by membrane-type 1 metalloproteinase or 4-aminophenylmercuric acetate
-
-
?
protease-activated receptor-1 + H2O
?
-
activation
-
-
?
protease-activated receptor-2 + H2O
?
-
activation
-
-
?
protease-activated receptor-3 + H2O
?
-
activation
-
-
?
protease-activated receptor-4 + H2O
?
-
activation
-
-
?
proteinase-activated receptor 1 + H2O
?
-
-
-
-
?
proteinase-activated receptor PAR-1 + H2O
activated proteinase-activated receptor PAR-1
-
no activation by mesotrypsin in epithelial cells, but mesotrypsin activates PAR-1 in astrocytoma cells. Half-maximal response for both cationic and anionic trypsin at about 10-20 nM
-
-
?
proteinase-activated receptor PAR-2 + H2O
activated proteinase-activated receptor PAR-2
-
no activation by mesotrypsin in epithelial cells. Half-maximal response for both cationic and anionic trypsin at about 5 nM
-
-
?
proteinase-activated receptor PAR-3 + H2O
activated proteinase-activated receptor PAR-3
-
no activation by mesotrypsin in epithelial cells. Half-maximal response for both cationic and anionic trypsin at about 10-20 nM
-
-
?
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
?
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide + H2O
?
-
-
-
-
?
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Leu + 7-amino-4-methylcoumarin
-
-
-
?
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Lys + 7-amino-4-methylcoumarin
-
-
-
?
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O
?
-
-
-
-
?
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
-
-
?
succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Trp + 7-amino-4-methylcoumarin
-
-
-
?
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Tyr + 7-amino-4-methylcoumarin
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-7-amido-4-methylcoumarin + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 7-amino-4-methylcoumarin
-
-
-
-
?
superoxide dismutase 1 + H2O
?
-
-
-
-
?
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin + H2O
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
?
-
-
-
?
tosyl-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
-
-
?
tosyl-Gly-Pro-Lys-4-methylcoumarin 7-amide + H2O
?
-
-
-
-
?
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin + H2O
?
-
-
?
tosyl-GPK-p-nitroanilide + H2O
tosyl-GPK + p-nitroaniline
tosyl-GPR-p-nitroanilide + H2O
tosyl-GPR + p-nitroaniline
ubiquitin + H2O
?
-
-
-
-
?
VP4 + H2O
fragments VP8* and VP5*
-
outer layer protein of rotavirus capsid
-
-
?
Z-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
Z-Ala-Arg-Arg + 7-amino-4-methylcoumarin
-
low amidolytic activity
-
-
?
Z-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg + 7-amino-4-methylcoumarin
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg-Arg + 7-amino-4-methylcoumarin
-
low amidolytic activity
-
-
?
Z-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
Z-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
low amidolytic activity
-
-
?
Z-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Z-Gly-Pro-Arg + 7-amino-4-methylcoumarin
Z-Gly-Pro-Arg-p-nitroanilide + H2O
Z-Gly-Pro-Arg + p-nitroaniline
-
-
-
-
?
Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
-
low activity
-
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
-
high amidolytic activity
-
-
?
additional information
?
-
2-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGHR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGHR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGLR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGLR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGMR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGMR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGNR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGNR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGTR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
2-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
2-aminobenzoyl-AGTR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
benzoyl-Arg-p-nitroanilide + H2O
benzoyl-Arg + p-nitroaniline
-
-
-
-
?
benzoyl-Arg-p-nitroanilide + H2O
benzoyl-Arg + p-nitroaniline
-
-
-
-
?
benzoyl-DL-Arg-4-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
benzoyl-DL-Arg-4-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
benzoyl-DL-Arg-4-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
benzoyl-DL-Arg-4-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
benzoyl-DL-Arg-4-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
benzoyl-L-arginine-p-nitroanilide + H2O
benzoyl-L-arginine + p-nitroaniline
-
-
-
-
?
benzoyl-L-arginine-p-nitroanilide + H2O
benzoyl-L-arginine + p-nitroaniline
-
-
-
-
?
benzyloxycarbonyl-Lys-thiobenzyl ester + H2O
benzyloxycarbonyl-Lys + phenylmethanethiol
-
-
-
-
?
benzyloxycarbonyl-Lys-thiobenzyl ester + H2O
benzyloxycarbonyl-Lys + phenylmethanethiol
-
-
-
-
?
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonylglycyl-L-prolyl-L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonylglycyl-L-prolyl-L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
Bovine serum albumin + H2O
?
-
-
-
-
?
Bovine serum albumin + H2O
?
-
-
-
?
Bovine serum albumin + H2O
?
-
-
-
-
?
bovine skin DQ-collagen type I + H2O
?
-
-
-
-
?
bovine skin DQ-collagen type I + H2O
?
-
-
-
-
?
carbobenzoxy-L-Arg-7-amido-4-methylcoumarin + H2O
carbobenzoxy-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
carbobenzoxy-L-Arg-7-amido-4-methylcoumarin + H2O
carbobenzoxy-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
carbobenzoxy-L-Arg-7-amido-4-methylcoumarin + H2O
carbobenzoxy-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
carbobenzoxy-L-Arg-7-amido-4-methylcoumarin + H2O
carbobenzoxy-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
casein + H2O
?
-
-
-
-
?
casein + H2O
?
-
beta-casein, mutant enzymes K188E and K188Y cleave nearly 30 new peptide bonds compared to wild-type enzyme
-
-
?
Cbz-L-Arg-7-amido-4-methylcoumarin + H2O
Cbz-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Cbz-L-Arg-7-amido-4-methylcoumarin + H2O
Cbz-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Cbz-L-Arg-7-amido-4-methylcoumarin + H2O
Cbz-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Cbz-L-Arg-7-amido-4-methylcoumarin + H2O
Cbz-L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Cytochrome c + H2O
?
-
-
-
-
?
Cytochrome c + H2O
?
-
-
-
?
epithelial sodium channel + H2O
?
-
-
epithelial sodium channel cleavage and activation by isoform trypsin IV but not by trypsin I requires a critical cleavage site, i.e. Lys189 in the extracellular domain of the gamma-subunit
-
?
epithelial sodium channel + H2O
?
-
trypsin activates epithelial sodium channels in native renal tubules
-
-
?
Hemoglobin + H2O
?
-
-
-
-
?
Hemoglobin + H2O
?
-
-
-
?
human cartilage collagen type II + H2O
?
-
-
-
-
?
human cartilage collagen type II + H2O
?
-
use of enzyme and its regulators as markers of prognosis and disease activity in rheumatoid arthritis
-
-
?
human placenta DQ-collagen type IV + H2O
?
-
-
-
-
?
human placenta DQ-collagen type IV + H2O
?
-
-
-
-
?
insulin + H2O
?
-
oxidized and S-aminomethylated B-chain of trypsin. The enzyme hydrolyzes peptide bonds in which the carbonyl group is contributed by Arg, Lys and S-2-aminoethyl-cysteine
-
-
?
insulin + H2O
?
-
S-sulfonated B-chain of bovine insulin, only the linkages Arg22-Gly23 and Lys29-Ala30 are hydrolyzed
-
-
?
insulin + H2O
?
-
oxidized A chain, and B chain
-
-
?
intestinal alkaline sphingomyelinase bound to mucosa + H2O
free intestinal alkaline sphingomyelinase
-
cleavage at R440, first tryptic site upstream of signal anchor. Physiological role of enzyme in sphingomyelin digestion
-
-
?
intestinal alkaline sphingomyelinase bound to mucosa + H2O
free intestinal alkaline sphingomyelinase
-
cleavage at R440, first tryptic site upstream of signal anchor
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
-
trypsin-specific substrate
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-DL-arginine-4-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-DL-arginine-4-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + p-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + p-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Arg ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
-
-
-
-
?
N-alpha-benzoyl-L-Arg ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
-
-
-
-
?
N-alpha-benzoyl-L-Arg methyl ester + H2O
N-alpha-benzoyl-L-Arg + methanol
-
-
-
-
?
N-alpha-benzoyl-L-Arg methyl ester + H2O
N-alpha-benzoyl-L-Arg + methanol
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
N-alpha-benzoyl-L-Arg-ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
-
-
-
-
?
N-alpha-benzoyl-L-Arg-ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
?
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
?
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
?
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-arginine + ethanol
-
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-arginine + ethanol
-
-
-
-
?
N-alpha-benzoyl-L-Lys-p-nitroanilide + H2O
N-alpha-benzoyl-L-Lys + p-nitroaniline
Streptomyces erythraeus trypsin has more than 1 order of magnitude greater amidase activity than mammalian trypsin
-
-
?
N-alpha-benzoyl-L-Lys-p-nitroanilide + H2O
N-alpha-benzoyl-L-Lys + p-nitroaniline
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + methanol
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + methanol
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
a synthetic trypsin substrate
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
-
a synthetic trypsin substrate
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
-
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
-
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
-
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
-
-
-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide + H2O
N-carbobenzyloxy-Gly-Pro-Arg + p-nitroaniline
-
-
-
-
?
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide + H2O
N-carbobenzyloxy-Gly-Pro-Arg + p-nitroaniline
-
-
-
-
?
Nalpha-4-tosyl-L-arginine methyl ester + H2O
Nalpha-4-tosyl-L-arginine + methanol
-
-
-
-
?
Nalpha-4-tosyl-L-arginine methyl ester + H2O
Nalpha-4-tosyl-L-arginine + methanol
-
-
-
-
?
Nalpha-4-tosyl-L-arginine methyl ester + H2O
Nalpha-4-tosyl-L-arginine + methanol
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
-
Nalpha-benzoyl-L-Arg-p-nitroanilide
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
-
Nalpha-benzoyl-L-Arg-p-nitroanilide
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-arginine + ethanol
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-arginine + ethanol
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-arginine + ethanol
-
-
-
-
?
Nalpha-p-tosyl-L-Arg methyl ester + H2O
?
-
-
-
?
Nalpha-p-tosyl-L-Arg methyl ester + H2O
?
-
-
-
-
?
Nalpha-p-tosyl-L-arginine methyl ester + H2O
?
-
-
-
-
?
Nalpha-p-tosyl-L-arginine methyl ester + H2O
?
-
-
-
-
?
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AAGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AAGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AAGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AAGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ADGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ADGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ADGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AEGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AEGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AEGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AEGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AFGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AFGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AFGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGAR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGAR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGAR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGAR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGDR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGDR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGDR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGDR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGER + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGER + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGER + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGER + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGFR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGFR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGFR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGFR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGK + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGK + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGK + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGK + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGGRGGGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GGGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGGRGGGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GGGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGHR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGHR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGHR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGHR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGIR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGIR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGIR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGIR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGKR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGKR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGKR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGKR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGLR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGLR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGLR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGMR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGMR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGMR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGMR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGNR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGNR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGNR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGNR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGQR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGQR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGQR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGQR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGSR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGSR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGSR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGSR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGTR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGTR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGTR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGVR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGVR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGVR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGVR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGYR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGYR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGYR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGYR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AHGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AHGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AIGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AIGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AIGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AIGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AKGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AKGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AKGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AKGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ALGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ALGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ALGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ALGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AMGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AMGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AMGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AMGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ANGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ANGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ANGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AQGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AQGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AQGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AQGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ASGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ASGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ASGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ATGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ATGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AVGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AVGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AVGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AVGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AYGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AYGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
?
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AYGR + GAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
-
-
?
p-nitrophenyl-p'-guanidino benzoate + H2O
p-nitrophenol + p-guanidinobenzoate
-
beta-trypsin is more reactive than alpha-trypsin
-
-
?
p-nitrophenyl-p'-guanidino benzoate + H2O
p-nitrophenol + p-guanidinobenzoate
-
-
-
?
p-tosyl-L-Arg methyl ester + H2O
?
-
-
-
-
?
p-tosyl-L-Arg methyl ester + H2O
?
Cambarus affinis
-
-
-
-
?
p-tosyl-L-Arg methyl ester + H2O
?
-
-
-
-
?
Tos-L-Arg-OMe + H2O
?
-
-
-
-
?
Tos-L-Arg-OMe + H2O
?
-
-
-
-
?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
?
-
-
-
?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
?
salmon
-
-
-
?
tosyl-GPK-p-nitroanilide + H2O
tosyl-GPK + p-nitroaniline
-
-
-
-
?
tosyl-GPK-p-nitroanilide + H2O
tosyl-GPK + p-nitroaniline
-
-
-
-
?
tosyl-GPR-p-nitroanilide + H2O
tosyl-GPR + p-nitroaniline
-
-
-
-
?
tosyl-GPR-p-nitroanilide + H2O
tosyl-GPR + p-nitroaniline
-
-
-
-
?
Z-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Z-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg + 7-amino-4-methylcoumarin
-
low amidolytic activity
-
-
?
Z-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Z-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
Z-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Z-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
high amidolytic activity
-
-
?
additional information
?
-
-
Asp189 is responsible for the specificity of the enzyme
-
-
?
additional information
?
-
-
no substrate: type II collagen
-
-
?
additional information
?
-
-
strong interaction of Phe in position P4 with enzyme
-
-
?
additional information
?
-
-
trypsin treatment increases zone of polarizing activity signaling of non-zone of polarizing activity tissue
-
-
?
additional information
?
-
-
does not hydrolyse D-Tat
-
-
?
additional information
?
-
-
trypsin is involved in the progesterone-induced acrosome reaction in canine spermatozoa
-
-
?
additional information
?
-
no substrates: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, Lys-7-amido-4-methylcoumarin and Arg-7-amido-4-methylcoumarin
-
-
?
additional information
?
-
-
no substrates: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, Lys-7-amido-4-methylcoumarin and Arg-7-amido-4-methylcoumarin
-
-
?
additional information
?
-
-
the purified trypsin from Colossoma macropomum shows substrate specificity for arginine at P1, efficiently hydrolizing substrates with leucine and lysine at P2 and serine and arginine at P10
-
-
?
additional information
?
-
-
does not hydrolyze o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine), and o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
?
additional information
?
-
-
the enzyme from catayzes also the reverse reaction and synthesizes Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide from Nalpha-(tert-butoxycarbonyl)-L-alanine-4-guanidinophenyl ester as acyl donor and L-alanine-p-nitroanilide (L-Ala-pNA) as acyl acceptor, overview
-
-
?
additional information
?
-
-
identification of autolytic cleavage sites within the trypsin I molecule, overview
-
-
?
additional information
?
-
-
trypsin-catalyzed peptide synthesis with inverse substrates as acyl donor components, overview
-
-
?
additional information
?
-
-
trypsin-catalyzed peptide synthesis using 4-amidino- and 4-guanidinophenyl esters of Nalpha-tert-butyloxycarbonyl-amino acid as acyl donor components with two types of acyl acceptors, overview
-
-
?
additional information
?
-
-
capable of agglutinating both bloodstream-form and procyclic trypanosomes as well as rabbit red blood cells
-
-
?
additional information
?
-
very low activity with dietary protein and azocasein
-
?
additional information
?
-
-
very low activity with dietary protein and azocasein
-
?
additional information
?
-
-
enzyme pancreatic isoforms cationic trypsin and anionic trypsin show activity on human proteinase-activated receptors PAR-1, PAR-2, PAR-3 comparable to that of bovine pancreatic trypsin. Mesotrypsin cannot activate or disable proteinase-activated receptors in human epithelial cells, but activates PAR-1 in human astrocytoma cells
-
-
?
additional information
?
-
-
does not react with proteinase-activated receptor 2
-
-
?
additional information
?
-
-
exhibits a relatively high specificity for Lys/Arg-Ser/Thr peptide bonds
-
-
?
additional information
?
-
-
active 1918 pandemic flu viral neuraminidase is resistant to trypsin digestion
-
-
?
additional information
?
-
-
does not cleave enhanced green fluorescent protein-T1m and wild type enhanced green fluorescent protein
-
-
?
additional information
?
-
-
the sequence of trypsin is IVGGY
-
-
?
additional information
?
-
-
cleavage of the potentially inhibitory peptides by isozyme trypsin-3, substrate specificity, overview
-
-
?
additional information
?
-
-
trypsin shows protease and esterase activities
-
-
?
additional information
?
-
-
Arg-4-methylcoumaryl-7-amide and Suc-Leu-Leu-Val-Lys-4-methylcoumaryl-7-amide are poor substrates, substrate specificities of trypsin A and B, overview
-
-
?
additional information
?
-
-
does not hydrolyze o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) and o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
-
?
additional information
?
-
trypsin specifically cleaves the peptide bond on the carboxyl side of Lys and Arg residues. It is characterized by a common catalytic mechanism involving the catalytic triad of three essential amino acid residues His57, Asp102, and Ser195 and a substrate determinant residue Asp189
-
-
?
additional information
?
-
-
trypsin treatment alters a number of characteristics of KATP channel pharmacology, this may be due to action at possibly more than one site but includes the functional cleavage of the sulfonylurea receptor from the KATP channel
-
-
?
additional information
?
-
-
salmon trypsin induces interleukin-8 production in human airway epithelial cells, purified salmon trypsin activates nuclear factor-kappa B-mediated gene expression in HeLa cells overexpressing human protease-activated receptor-2, the production of interleukin-8 by salmon trypsin is mediated by protease-activated receptor-2
-
-
?
additional information
?
-
-
three-dimensional structure and topological specificities, substrate recognition, overview. The neighboring residues Leu71 and Gln72 in the N-terminal beta-barrel domain of the enzyme synergistically play an important role in substrate recognition. Identification of amino acid residue(s) related to collagen binding for wild-type and mutant enzymes
-
-
?
additional information
?
-
-
three-dimensional structure and topological specificities, substrate recognition in which residue 71 plays a crucial role, overview. Identification of amino acid residue(s) related to collagen binding of wild-type and mutant enzymes
-
-
?
additional information
?
-
-
the active centre is the catalytic triad: His57, Ser195, Asp102
-
-
?
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(2R,4R)-4-phenyl-1-[Nalpha-(7-methoxy-2-naphthalenesulfonyl)-L-arginyl]-2-piperidinecarboxylic acid
competitive, small molecular weight inhibitor without tryptase inhibitor activity
(4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl)-(3,4,5-6-tetrahydro[2H-1,4']bipyridinyl-4yl)-methanone
-
-
1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane
1-[N2-[(7-methoxynaphthalen-2-yl)sulfonyl]-L-arginyl]-4-phenylpiperidine-2-carboxylic acid
IC50: 0.0003 mM
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one
-
-
2,7-bis(4-amidinobenzylidene)cycloheptan-1-one
-
unspecific strong inhibition
2-mercaptoethanol
-
0.1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity
3,4-dichloroisocoumarin
-
-
4-(2-aminoethyl)-benzensulfonylfluoride
-
isozyme TryIII activity is completely (more than 95%) inhibited by 4-(2-aminoethyl)-benzensulfonylfluoride
4-(amidinophenyl)methanesulfonyl fluoride
-
-
6-amidino-2-naphthol
-
mixed-type inhibition
acetonitrile
-
partial inhibition at 20% acetonitrile, a 5% aqueous acetonitrile solution less likely reduces the activity of the porous polymer monolith-immobilized trypsin
acyclic SFTI
-
i.e. oSFTI, modified sunflower trypsin inhibitor 1, SFTI-1, generated by Fmoc-based automated synthesis
acyclic sun flower trypsin inhibitor-1
-
-
-
Adenanthera pavonina trypsin inhibitor
-
Adunil CEL
-
0.1% v/v, 47.3% residual activity
-
alpha-1-antitrypsin
-
in gestational diabetes mellitus reduction of serum trypsin inhibitory capacity may be due to non-enzymatic glycosylation of alpha-1-antitrypsin or oxidation of methionine in the active site of alpha-1-antitrypsin
-
alpha1-antitrypsin Pittsburgh
-
effective inhibitor, variant of alpha1-antitrypsin containing an Arg residue in place of the P1 Met358 in the reactive-site peptide bond
-
Apios Americana trypsin inhibitor
-
AATI, strong inhibitor, complete inhibition with Apios Americana trypsin inhibitor bound to trypsin in a 1:2 molar ratio
-
atropine
-
strongly diminishes trypsin activity in stressed animals
AVNIPFKVHFRCKAAFC
-
a small trypsin inhibitor from the skin secretion of the frog Odorrana grahami, only inhibits the hydrolysis activity of trypsin on synthetic chromogenic substrate
Bauhinia variegata var. variegata trypsin inhibitor
-
i.e. BvvTI, a Kunitz-type inhibitor from seeds of the Camels foot tree, Bauhinia variegata var. variegata. The inhibitor is also capable of significant inhibition of the proliferation of nasopharyngeal cancer CNE-1 cells in a selective way and of inhibiting anti-HIV-1 reverse transcriptase activity, overview
-
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-ethylphenyl)phosphonate
-
50% inhibition at 0.000017 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-isopropylphenyl)phosphonate
-
50% inhibition at 0.000061 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-methylthiophenyl)phosphonate
-
50% inhibition at 0.0000087 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-t-butylphenyl)phosphonate
-
50% inhibition at 0.000088 mM, comparison with inhibitory effect on urokinase
bis(m-amidinosalicylidene-L-alaninato)iron(III)
-
-
bis(p-amidinosalicylidene-L-alaninato)iron(III)
-
-
-
bisphenol A
-
the secondary and tertiary structures of trypsin are altered by bisphenol S binding, which results in the loosening of the skeleton of the enzyme. Bisphenol S induces microenvironmental changes around tyrosine and tryptophan residues of trypsin. The activity of trypsin does not change remarkably with the increasing concentration of bisphenol S. The binding of bisphenol S to trypsin is a spontaneous process and hydrogen bonding and hydrophobic interactions play a vital role in stabilizing the bisphenol S-trypsin complex. The binding constants of bisphenol S with trypsin are 74200 (25°C) and 59100 L/mol (37°C)
black gram trypsin inhibitor 1
-
highly effective trypsin inhibitor, 87.5% of trypsin inhibitory activity of black gram trypsin inhibitor 1 is retained after treatment with 10 mM dithiothreitol for 2 hours, and the activity dwindles to 12.5% after treatment with 100 mM dithiothreitol for 2 hours
-
black gram trypsin inhibitor 2
-
highly effective trypsin inhibitor, the trypsin inhibitory activity of black gram trypsin inhibitor 2 is unaffected after exposure to 100 mM dithiothreitol for 2 hours
-
black gram trypsin inhibitor 3
-
highly effective trypsin inhibitor, in the presence of 10 mM dithiothreitol trypsin inhibitory of black gram trypsin inhibitor 3 drops to 57.1% after 1 h and becomes undetectable after 2 hours, while treatment of 100 mM dithiothreitol for 1 h has the same effect
-
Bovine pancreatic trypsin inhibitor
-
bovine secretory inhibitors
-
A and B
-
BPTI-20st
-
simplified variant of bovine pancreatic trypsin inhibitor containing 20 alanines, retains a wild type level of trypsin inhibitory activity
-
BPTI-[5,55]st
-
single-disulfide-bonded variant of bovine pancreatic trypsin inhibitor, retains a wild type level of trypsin inhibitory activity
-
captopril
-
feeding angiotensin converting enzyme inhibitor captopril down-regulates trypsin activity in the larval gut, injecting third instar larvae with a combination of Aedes aegypti trypsin-modulating oostatic factor and captopril downregulates trypsin biosynthesis in the larval gut
Cd2+
-
10 mM, 48% inhibition
clispin
-
a Clitocybe nebularis trypsin inhibitor variant, recombinantly expressed in Escherichia coli, highly specific towards trypsin, fast-acting, and tight-binding. Molecular cloning of the gene and cDNA encoding cnispin, overview
Clitocybe nebularis trypsin inhibitor
-
CnSPI, several inhibitor variants, separation by trypsin affinity chromatography. Possible regulatory role for CnSPIs in the endogenous proteolytic system of Clitocybe nebularis. Isolation from frozen fruiting bodies or basidiocarps
-
cyclic SFTI
-
i.e. cSFTI, modified sunflower trypsin inhibitor 1, SFTI-1, generated by Fmoc-based automated synthesis
dithiothreitol
-
1 mM, no residual activity
DMTI-II
-
trypsin inhibitor from seeds of Dimorphandra mollis. The inhibitory activity is stable over a wide range and in the presence of DTT
-
domesticated soybean inhibitor
-
SBTI
-
DOTA-SFTI
-
modified sunflower trypsin inhibitor 1, SFTI-1, labeled, generated by Fmoc-based automated synthesis
Ecballium elaterium trypsin inhibitor II
-
EETI, a a model member of the knottin family, used for construction of libraries of EETI loop-substituted variants with diversity in both amino acid sequence and loop length, wild-type EETI or loop-substituted clones expressed from yeast, structure-function analysis, modelling, overview
-
enalapril
-
feeding angiotensin converting enzyme inhibitor enalapril down-regulates trypsin activity in the larval gut
ethylenediaminetetraacetic acid
-
partial
Gly-L-Arg-(2-amino butanoyl)-L-Thr-Nphe-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp
-
-
Gly-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
-
sun flower trypsin inhibitor SFTI-1
Glycine max trypsin inhibitor
-
from raw soymilk, inhibition kinetics, overview
-
I-ovalbumin
-
high affinity interaction of enzyme with I-ovalbumin, the product of a heating transition of ovalbumin which acts as a potent reversible serine proteinase inhibitor. Interaction is characterized by high kinetic association constants and low kinetic dissociation konstants
-
iodoacetic acid
16.5% inhibition at 1 mM; 1 mM, 16.5% inhibition
K+
-
10 mM, 16% inhibition
Kunitz domain 1 of tissue factor pathway inhibitor-2
-
-
-
Kunitz type trypsin inhibitor from Glycine max
-
isolated and purified from Korean large black soybeans cultivar, overview. Inhibits bovine trypsin, but also shows Anti-HIV reverse transcriptase activity, cytokine-inducing activity and antiproliferative activity on tumor cell lines
-
L-1-chloro-3-tosylamido-4-phenyl-2-butanone
-
-
L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone
-
-
L-Lys-L-Cys-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
-
-
leech-derived tryptase inhibitor
-
i.e. LDTI, purely competitive inhibitor
-
LEKTI
-
the LEKI gene encodes a 15-domain serine proteinase inhibitor, disulfide bonds are important for LEKTI function, noncompetitive mechanism, expression and purification of human LEKTI gene in Sf9
Li+
-
10 mM, 19% inhibition
lily bulb trypsin inhibitor
-
-
-
Lima bean trypsin inhibitor
-
Locusta migratoria protease inhibitor 1
-
strong inhibition
-
Locusta migratoria protease inhibitor 3
-
strong inhibition, the inhibitor interacts with trypsin through the reactive site P3-P4' and the P10-P6 residues
-
low-molecular-mass trypsin inhibitor inhibitor type-2
-
from Sinapis alba, inhibitor consists of a peptide mixture, displaying Ile or Arg at position 43, Trp or kynurenine at position 44, and C-terminal ragged ends
-
m-amidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
m-amidinosalicylidene-L-alaninato(aqua)copper(II) hydrochloride
-
-
-
m-guanidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
mung bean trypsin inhibitor
-
mustard trypsin inhibitor 2
-
mustard trypsin inhibitor II
inhibition of HzTrypsin-C, no inhibition of HzTrypsin-S
-
N-alpha-p-tosyl-L-lysine chloromethyl ketone
N-alpha-tosyl-L-Lys-chloromethyl ketone
-
complete inhibition at 1 mM
N-alpha-tosyl-L-lysine chlormethylketone
-
less than 50% inhibition of isozyme TryIII by the trypsin-specific inhibitor N-alpha-tosyl-L-lysine chlormethylketone
N-alpha-tosyl-L-lysine chloromethyl ketone
-
-
N-alpha-tosyl-L-lysine-chloromethylketone
-
complete inhibition at 5 mM
N-p-tosyl-L-lysine chloromethyl ketone
N-p-tosyl-lysine chloroketone
N-tosyl-L-lysine chloromethyl ketone
82.9% inhibition at 5 mM
N-tosyl-L-lysine-chloromethyl ketone
N-tosyl-L-phenyl-alanine-chloromethyl ketone
-
11% inhibition at 1mg/ml
N-tosyl-L-phenylalanine-chloromethyl ketone
-
5.34% inhibition at 5 mM
Na+
-
10 mM, 21% inhibition
Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride
-
inhibits trypsin specifically and irreversibly, 40% reduced activity in the presence of 1 mM Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride
Nafamostat
-
potent inhibitor
nafamostat mesilate
IC50: 0.00003 mM
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-acetyl-piperazide
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-methylpiperidide
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid methyl ester
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid methylester
-
-
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-piperazide
-
-
Nalpha-p-tosyl-L-lysine chloromethyl ketone
-
complete inhibition
Nalpha-tosyl-L-Lys-chloromethyl ketone
Nalpha-tosyl-L-Lys-chloromethylketone
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester
-
-
napsagatran ethylester
-
-
nexin-1
-
nexin-1 inhibits isozymes trypsin-1 ad trypsin-2, nexin-1 inhibits trypsin-4 and forms stable complexes only with this trypsin isoenzyme
-
p-amidinosalicylidene-L-alaninato(aqua)copper(II)
-
-
p-nitrophenyl-p'-guanidino-benzoate
-
irreversible trypsin inhibitor
p-tosyl-L-Lys-chloromethane
pancreatic secretory trypsin inhibitor
-
Pancreatic trypsin inhibitor
-
pepstatin A
26% inhibition at 0.01 mM
Phaseolus vulgaris trypsin inhibitor
-
two different variants of 132 kDa and 118 kDA from navy beans and red kidney beans, respectively, isolated by heat treatment and ammonium sulfate fractionation
-
phenyl methyl sulphonyl fluoride
-
100% inhibition
phenylmethyl sulfonyl fluoride
phenylmethylsulfonyl fluoride
Phenylmethylsulfonylfluoride
Plathymenia foliolosa trypsin inhibitor
-
PFTI
-
polylysine
-
immobilized enzyme
porcine secretory inhibitors
-
-
-
porcine trypsin inhibitor
-
0.01 mM, more than 95% inhibition
-
potato inhibitor II
strong inhibition of HzTrypsin-C, no inhibition of HzTrypsin-S
-
Protease Inhibitor Cocktail
-
-
-
Putranjiva roxburghii trypsin inhibitor
-
highly potent inhibitor of bovine trypsin, trypsin inhibitory activity of Putranjiva roxburghii trypsin inhibitor is completely retained up to 70°C, above 70°C, there is a slight decrease in the inhibitory activity retaining almost 85% inhibitory activity up to 80°C, the inhibitory activity of Putranjiva roxburghii trypsin inhibitor falls sharply above 80°C with a loss of almost 80% inhibitory activity at 90°C, only a slight decrease of 5% in inhibitory activity is observed when Putranjiva roxburghii trypsin inhibitor is incubated for 2 h at 100 mM dithiothreitol
-
Sagittaria sagittifolia arrowhead protease inhibitor A
-
API-A
-
small glossy black soybean trypsin inhibitor
-
inhibitory activity is stable in the pH range 3-13 and in the temperature range 0-60°C, is inhibited by dithiothreitol (5-25 mM) in a dose-dependent manner
-
sodium dodecylsulfate
-
0.5% w/v, no residual activity
soybean Bowman-Birk inhibitor
inhibition of HzTrypsin-C,no inhibition of HzTrypsin-S
-
soybean Kunitz trypsin inhibitor
-
Soybean trypsin inhibitor
-
Spinacia oleracea trypsin inhibitor
-
high affinity between trypsin and Spinacia oleracea trypsin inhibitor
-
sun flower trypsin inhibitor-1
-
-
sunflower trypsin inhibitor 1
-
SFTI-1, binding and effects on human prostate cancer cells, overview
sunflower trypsin inhibitor-1
SVIGCWTKSIPPRPCFVK-amide
-
HV-BBI is a Bowman-Birk type protease inhibitor from the skin secretion of the Chinese Bamboo odorous frog Huia versabilis, SVIGCWTKSIPPRPCFVK-amide is a synthetic replicate of HV-BBI with the wild-type K (Lys8) residue in the presumed P1 position and is a potent, competitive, and reversible inhibitor of trypsin
SVIGCWTRSIPPRPCFVK-amide
-
HV-BBI is a Bowman-Birk type protease inhibitor from the skin secretion of the Chinese Bamboo odorous frog Huia versabilis, SVIGCWTRSIPPRPCFVK-amide is a synthetic replicate of HV-BBI with the mutant R (Arg8) residue in the presumed P1 position and is a competitive, and reversible inhibitor of trypsin with reduced potency compared to the wild type peptide SVIGCWTKSIPPRPCFVK-amide
T5E/N18R/T20G/P21S/T22D/K31M mutant Schistocerca gregaria protease (trypsin) inhibitor 1
-
the mutant inhibitor is improved compared to the wild-type inhibitor protein
-
Tamarindus indica trypsin inhibitor
-
a Kunitz-type inhibitor
-
thionine
-
from cowpea, inhibits trypsin, no inhibition of chymotrypsin
tissue inhibitor of matrix metalloproteinases-2
-
TIMP-2, prevents the C-terminal truncation of activated metalloproteinase-2, without affecting the generation of the initial 62 kDa activated species
-
tosyl-L-lysine-chloromethyl ketone
-
-
tosyl-L-lysyl chloromethyl ketone
tosyl-L-Phe chloromethyl ketone
-
-
trans-epoxysuccinyl-L-leucylamido-(4-guanidino) butane
-
-
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
-
trypsin inhibitor I-S Type from soybean
-
reversible trypsin inhibitor
-
trypsin modulating oostatic factor
-
the hexapeptide NPTNLH is secreted at the last stage of vitellogenesis and inhibits the biosynthesis of trypsin in insect midgut cells
-
tumor-associated trypsin inhibitor
-
urinary trypsin inhibitor
-
Vigna angularis trypsin inhibitor
-
from adzuki beans, 13 kDa, isolated by heat treatment and ammonium sulfate fractionation
-
Vigna mungo trypsin inhibitor
-
purification of the Bowman-Birk proteinase inhibitor from the seeds of black gram, Vigna mungo cv. TAU-1. 8041.5 Da by mass spectrometry, pI 4.3-6.0, stable up to 80°C and at pH 2.0-12.0, analysis of the secondary structural conformation, overview, exhibts non-competitive-type inhibitory activity against both bovine pancreatic trypsin
-
wild-type soybean inhibitor
-
WBTI
-
[4-(6-chloro-naphthalene-2-sulphonyl)piperazin-1-yl]-(3,4,5,6-tetrahydro-[2H-1,4']bipyridinyl-4-yl)-methanone
-
-
[Abu(3, 11)]-sun flower trypsin inhibitor-1
-
-
-
[Arg5]-SFTI-1
-
a wild-type SFTI-1 analogue
[Lys5]-SFTI-1
-
a wild-type SFTI-1 analogue
[N,N'-bis(m-amidinosalicylidene)ethylenediaminato]copper(II)
-
-
-
[N,N'-bis(m-guanidinosalicylidene)ethylenediaminato]copper(II)
-
-
-
[Phe5]-SFTI-1
-
a wild-type SFTI-1 analogue
1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane
16.9% inhibition at 0.1 mM
1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane
-
6.09% inhibition at 0.1 mM
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of larvae of Anticarsia gemmatalis, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of Corcyra cephalonica, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of Diatraea saccharalis, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of Anagasta kuehniella, a severe pest in flour mills, at 1% with a significant reduction in larval survival and weight, effects of this inhibitor on food consumption, absorption and utilization, as well as the effects of the inhibitor on the midgut proteolytic activity of larvae fed on an artificial diet, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, has only slight retarding effect on larval and pupal developmental and growth of Sitotroga cerealella, overview
-
Adenanthera pavonina trypsin inhibitor
-
i.e. ApTI, isolated and purified from seeds, retards larval and pupal developmental and growth of Spodoptera frugiperda, overview
-
antipain
IC50: 0.04 mM
antipain
-
0.01 mM, isoenzyme aT-I loses 96% of its activity, isoenzyme aT-II loses 99% of its activity
Aprotinin
-
-
Aprotinin
-
immobilized enzyme
Aprotinin
-
very efficient inhibition of trypsin-1 and trypsin-2, but not of trypsin-4
Ba2+
-
-
Ba2+
-
27% inhibition at 5 mM
benzamidine
-
unspecific, weak inhibition
benzamidine
-
competitive inhibitor
benzamidine
5 mM, 17.9% residual activity
benzamidine
-
99% inhibition at 1 mM
benzamidine
-
1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity
benzamidine
-
72.0% inhibition of trypsin A, 95.4% of trypsin B, at 1 mM. 88.8% inhibition of trypsin A, 97.6% of trypsin B at 0.1 mM,
benzamidine
-
54% inhibition at 5 mM
benzamidine
-
5 mM, 22% residual activity
benzamidine
-
80.2% inhibition
benzamidine
-
complete inhibition
benzamidine
-
55% inhibition at 5 mM
benzylamine
-
-
Bovine pancreatic trypsin inhibitor
-
-
-
Bovine pancreatic trypsin inhibitor
-
BPTI
-
Bovine pancreatic trypsin inhibitor
-
-
-
Bovine pancreatic trypsin inhibitor
-
i.e. BPTI or aprotinin, influence of temperature on the relationship between structure and dynamics of the inhibitor protein, calculations and X-ray crystal structure of BPTI at 1.5 A resolution, global diffusion and internal motions, overview
-
Bovine pancreatic trypsin inhibitor
-
BPTI, a natural non-specific serine protease inhibitor which possesses the ability to inhibit trypsin, the inhibitory activity of recombinant BPTI against trypsin is the same as natural BPTI, the trade name is aprotinin
-
Bovine pancreatic trypsin inhibitor
-
also known as aprotinin. At the lowest aprotinin concentration of 0.000625 mg, the residual trypsin obtained in neonate larvae of shoot borer, internode borer and top borer is 33.6, 24.8 and 9.3%, respectively. The residual trypsin for third instar of shoot borer, internode borer and top borer at the lowest aprotinin concentration of 0.000125 mg is 55.0, 65.0 and 20.7%, respectively. The amount of aprotinin needed to bring about 50% inhibition of gut proteinases of third instar larvae is 0.00025 mg for shoot borer and 0.0025 mg for internode borer whereas 0.000125 mg is sufficient to bring about nearly 80% inhibition in top borer.
-
Bowman-Birk inhibitor
-
double-headed native trypsin/chymotrypsin inhibitor in soybeans, which inhibits trypsin and chymotrypsin in a non-competitive manner, 78% reduced activity in the presence of 0.0005 mM Bowman-Birk inhibitor
Bowman-Birk inhibitor
-
-
chymostatin
-
-
Co2+
-
10 mM, 31.6% inhibition
Co2+
-
48% inhibition at 5 mM
Cu2+
-
14% inhibition at 5 mM
Cu2+
-
68% inhibition at 5 mM
DFP
-
-
E-64
0.02 mM, 19.5% residual activity
E-64
-
18.3% inhibition of trypsin A, 11.1% of trypsin B, at 0.001 mM. 46.9% inhibition of trypsin A, 33.8% of trypsin B at 0.01 mM
EDTA
-
10% inhibition at 2 mM
EDTA
-
12% inhibition at 5 mM
EDTA
-
weak inhibition, 18% inhibition at 10 mM
EDTA
14.2% inhibition at 2 mM
EDTA
-
50 mM, 88% residual activity
EDTA
-
1 mM, 53.9% residual activity
EDTA
-
6.85% inhibition at 2 mM
EDTA
-
a metalloenzyme inhibitor, 8% inhibition at 5 mM
Hg2+
-
57% inhibition at 5 mM
Hg2+
-
72% inhibition at 5 mM
Hg2+
-
10 mM, 85.7% inhibition
leupeptin
IC50: 0.0002 mM
leupeptin
-
0.1 mM, isoenzyme aT-I loses 97% of its activity, isoenzyme aT-II completely loses its activity
Lima bean trypsin inhibitor
0.002 mM, 7.9% residual activity
-
Lima bean trypsin inhibitor
-
-
-
Lima bean trypsin inhibitor
-
-
-
Mg2+
-
24% inhibition at 5 mM
Mg2+
-
10 mM, 21.6% inhibition
Mn2+
-
-
Mn2+
-
34.5% inhibition at 5 mM
mung bean trypsin inhibitor
-
synthesis and inhibitory activity of mutant inhibitor variants expressed in Escherichia coli, overview
-
mung bean trypsin inhibitor
0.003 mM, 3.6% residual activity
-
mustard trypsin inhibitor 2
-
-
-
mustard trypsin inhibitor 2
-
80% of trypsin activity can be inhibited by 42 nM mustard trypsin inhibitor 2
-
N-alpha-p-tosyl-L-lysine chloromethyl ketone
-
10 mM, 99.67% inhibition
N-alpha-p-tosyl-L-lysine chloromethyl ketone
-
85.4% inhibition
N-p-tosyl-L-lysine chloromethyl ketone
5 mM, 83% inhibition
N-p-tosyl-L-lysine chloromethyl ketone
-
88% inhibition at 5 mM
N-p-tosyl-L-lysine chloromethyl ketone
-
89% inhibition at 0.1 mM
N-p-tosyl-L-lysine chloromethyl ketone
-
complete inhibition at 5 mM
N-p-tosyl-lysine chloroketone
-
-
N-p-tosyl-lysine chloroketone
-
-
N-p-tosyl-lysine chloroketone
-
-
N-p-tosyl-lysine chloroketone
-
-
N-tosyl-L-lysine-chloromethyl ketone
-
82% inhibition at 5 mM
N-tosyl-L-lysine-chloromethyl ketone
-
complete inhibition
NaCl
-
enzyme activity continuously decreases with increasing NaCl concentration of 0-30%
NaCl
-
60& inhibition at 35% NaCl
NaCl
69% inhibition at 0.6 M
NaCl
-
enzyme activity continuously decreases to 50% of maximal activity with increasing NaCl concentration of 0-30%
NaCl
-
trypsin activity decreases continuously as NaCl concentration increases from 030%
NaCl
-
exhibits a progressive decrease in activity with increasing NaCl concentration (0-30%)
NaCl
-
relative activities of trypsin isozymes A, B, and C at 15% NaCl are approximately 39%, 38% and 40% of maximal activity, respectively
NaCl
-
60% inhibition at 30% NaCl
NaCl
-
activity decreases with increasing NaCl concentrations (0-30%)
Nalpha-tosyl-L-Lys-chloromethyl ketone
-
91% inhibition at 1 mM
Nalpha-tosyl-L-Lys-chloromethyl ketone
-
-
Nalpha-tosyl-L-Lys-chloromethyl ketone
-
-
Nalpha-tosyl-L-Lys-chloromethyl ketone
-
-
Nalpha-tosyl-L-Lys-chloromethyl ketone
-
-
Nalpha-tosyl-L-Lys-chloromethylketone
-
79% inhibition at 5 mM
Nalpha-tosyl-L-Lys-chloromethylketone
-
89% inhibition at 5 mM
Nalpha-tosyl-L-Lys-chloromethylketone
-
-
ovomucoid
-
-
-
ovomucoid
-
chicken ovomucoid
-
p-tosyl-L-Lys-chloromethane
-
-
p-tosyl-L-Lys-chloromethane
-
reaction towards B chain of insulin is inhibited, the activity towards the A-chain is not significantly affected
pancreatic secretory trypsin inhibitor
-
a Kazal-type specific trypsin inhibitor with narrow specificity for the inhibition of trypsin, with very little or no inhibitory capacity against other serine proteinases, even those exhibiting trypsin-like specificity
-
pancreatic secretory trypsin inhibitor
-
also known as serine protease inhibitor Kazal type 1
-
Pancreatic trypsin inhibitor
-
i.e. trasylol, kallikrein inactivator
-
Pancreatic trypsin inhibitor
-
bovine pancreatic trypsin inhibitor
-
PdKI-3.1
-
isolation of two highly pH- and thermo-stable Kunitz-type inhibitors of trypsin from seeds of the tree Pithecellobium dumosum, overview
-
PdKI-3.1
-
isolation of two highly pH- and thermo-stable Kunitz-type inhibitors of trypsin from seeds of the tree Pithecellobium dumosum, overview
-
PdKI-3.1
-
isolation of two highly pH- and thermo-stable Kunitz-type inhibitors of trypsin from seeds of the tree Pithecellobium dumosum, overview
-
PdKI-3.2
-
isolation of two highly pH- and thermo-stable Kunitz-type inhibitors of trypsin from seeds of the tree Pithecellobium dumosum, overview
-
PdKI-3.2
-
isolation of two highly pH- and thermo-stable Kunitz-type inhibitors of trypsin from seeds of the tree Pithecellobium dumosum, overview
-
PdKI-3.2
-
isolation of two highly pH- and thermo-stable Kunitz-type inhibitors of trypsin from seeds of the tree Pithecellobium dumosum, overview
-
Pefabloc SC
1 mM, 4.5% residual activity
Pefabloc SC
-
completely inhibits isoenzymes trypsin-1, trypsin-2, and trypsin-4
Pefabloc SC
-
complete inhibition of trypsin A and B at 1 mM
phenylbutylamine
-
-
phenylbutylamine
salmon
-
-
Phenylethylamine
-
-
Phenylethylamine
salmon
-
-
phenylmethyl sulfonyl fluoride
-
-
phenylmethyl sulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
10 mM, 4.5% residual activity
phenylmethylsulfonyl fluoride
-
100 mM, complete inhibition
phenylmethylsulfonyl fluoride
-
5 mM, 43% residual activity
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
a serine protease inhibitor, over 90% inhibition of trypsin isozymes A, B and C at 5 mM
phenylmethylsulfonyl fluoride
-
partial
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
-
Phenylmethylsulfonylfluoride
-
89.5% inhibition at 1 mM
Phenylmethylsulfonylfluoride
-
-
Phenylpropylamine
-
-
Phenylpropylamine
salmon
-
-
PMSF
-
80% inhibition at 1 mM, complete at 5 mM
PMSF
-
62% inhibition at 1 mM, complete inhibition at 5 mM
PMSF
-
73% inhibition at 1 mM
PMSF
-
0.1 mM, isoenzyme aT-I loses 89% of its activity, isoenzyme aT-II loses 80% of its activity
PMSF
-
80.7% inhibition of trypsin A, 57.4% of trypsin B at 0.1 mM. 96.4% inhibition of trypsin A, 93.2% of trypsin B at 1 mM
PMSF
-
89% inhibition at 5 mM
PMSF
-
complete inhibition
PMSF
-
61% inhibition at 1 mM, complete inhibition at 5 mM
soybean Kunitz trypsin inhibitor
-
-
-
soybean Kunitz trypsin inhibitor
strong inhibition of HzTrypsin-C, no inhibition of HzTrypsin-S
-
soybean Kunitz trypsin inhibitor
-
80% of trypsin activity can be inhibited by 42 nM soybean Kunitz trypsin inhibitor
-
Soybean trypsin inhibitor
-
complete inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
complete inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
complete inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
IC50: 0.000009 mM
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
different Kunitz trypsin inhibitors, KTIs, from different soybean lines, 15 soybean experimental lines and varieties, overview
-
Soybean trypsin inhibitor
-
isozyme TryIII activity is completely (more than 95%) inhibited by soybean trypsin inhibitor
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
250 mM, complete inhibition
-
Soybean trypsin inhibitor
-
84% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
0.1 mg/ml, isoenzyme aT-I loses 95% of its activity, isoenzyme aT-II loses 97% of its activity
-
Soybean trypsin inhibitor
1 mg/ml, 80% inhibition; 80.3% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
91% inhibition at 1mg/ml
-
Soybean trypsin inhibitor
-
80% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
complete inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
91.5% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
complete inhibition
-
Soybean trypsin inhibitor
-
0.025% w/v, 0.3% residual activity
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
96% inhibition at 1 mM
-
Soybean trypsin inhibitor
-
75.6% inhibition
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
-
Soybean trypsin inhibitor
-
84.2% inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
a serine protease inhibitor, complete inhibition of trypsin isozymes A, B and C at 1 mg/ml
-
Soybean trypsin inhibitor
-
complete inhibition
-
Soybean trypsin inhibitor
-
0.01 mM, more than 95% inhibition
-
Soybean trypsin inhibitor
-
SBT, complete inhibition at 1 mg/ml
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
-
-
Soybean trypsin inhibitor
-
a Kunitz trypsin inhibitor from soybean seeds, identification of mutants, several polymorphic types that are controlled by multiple alleles, overview. Development of a single nucleotide amplified polymorphism marker for the classification of the predominant KTi types, Tia and Tib, and evaluation of KTi activities by differing KTi type total 451 soybean mutant lines, overview. Trypsin inhibitor type and trypsin inhibitor activity in TIU/mg, overview
-
Soybean trypsin inhibitor
-
effective inhibitor
-
Soybean trypsin inhibitor
-
strong
-
Soybean trypsin inhibitor
-
-
-
sunflower trypsin inhibitor-1
-
SFTI-1
sunflower trypsin inhibitor-1
-
SFTI-1, a natural 14-residue cyclic peptide, can be used for enzyme separation from other serine proteases, synthesis of SFTI-1-based supports and affinity chromatography, overview. Inhibition of trypsin by some acyclic SFTI-1 analogues, e.g. [Lys5]-SFTI-1, [Arg5]-SFTI-1, and [Phe5]-SFTI-1, no inhibition by [Leu5]-SFTI-1, overview
tosyl-L-lysyl chloromethyl ketone
-
-
tosyl-L-lysyl chloromethyl ketone
-
-
tosyl-L-lysyl chloromethyl ketone
-
-
tosyl-L-lysyl chloromethyl ketone
-
-
tosyl-L-lysyl chloromethyl ketone
Triops sp.
-
-
tumor-associated trypsin inhibitor
-
specific inhibition of degradation of gelatin and collagen type II
-
tumor-associated trypsin inhibitor
-
specific inhibition of degradation of gelatin and collagen type II
-
tumor-associated trypsin inhibitor
-
very efficient inhibition of trypsin-1 and trypsin-2, but not of trypsin-4
-
tumor-associated trypsin inhibitor
-
i.e. TATI, endogenous inhjibitor expressed in the cytoplasm of colon mucosa and colorectal cancer cells, shows prognostic significance of tumour-specific TATI expression in colorectal cancer, inhibitor expression is associated with a significantly decreased overall survival of colon cancer patients, and with increased liver metastasis, overview
-
urinary trypsin inhibitor
-
also known as ulinastatin
-
urinary trypsin inhibitor
-
urinary trypsin inhibitor reduces nuclear factor-kappa B activation and downregulates the expression of its related mediators, followed by the inhibition of neutrophil aggregation and infiltration in hepatic ischemiareperfusion injury
-
urinary trypsin inhibitor
-
-
-
urinary trypsin inhibitor
-
also known as bikunin
-
urinary trypsin inhibitor
-
-
-
urinary trypsin inhibitor
-
a Kunitz type protease inhibitor
-
Zn2+
-
18% inhibition at 5 mM
Zn2+
-
10 mM, 31.6% inhibition
Zn2+
-
71% inhibition at 5 mM
additional information
-
no inhibition by E-64, N-ethylmaleimide, iodoacetic acid, pepstatin A, and Nalpha-4-tosyl-L-Phe-chloromethylketone
-
additional information
-
the enzyme activity is completely stable in presence of detergents, overview. No inhibition by EDTA, 2-nercaptoethanol, pepstatin A, Ca2+, Ba2+, Zn2+, Cu2+, Mg2+, Mn2+, K+, and Na+
-
additional information
-
no or poor inhibition by pepstatin A, 2-mercaptoethanol, Ca2+, K+, and Na+
-
additional information
-
naturally occurring trypsin inhibitor SFTI-1 isolated from sunflower seeds and its analogues
-
additional information
-
4-guanidinebenzoate: no inhibition up to 0.2 mM
-
additional information
-
trypsin is not inhibited by N-(4-aminobutyl)-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp and N-benzylglycine-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
-
additional information
-
trypsin is not inhibited by N-(4-aminobutyl)-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp and N-benzylglycine-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp, Gly-L-Arg-(2-amino butanoic acid)-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp, L-Lys-(2-amino butanoyl)-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp, and Nlys-(2-amino butanoyl)-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp
-
additional information
-
design and synthesis of Schiff base metal chelate inhibitors of trypsin, structural basis, overview. The binding mode of the guanidino groups of m-guanidinosalicylidene-L-alaninato(aqua)copper(II) hydrochloride and [N,N'-bis(m-guanidinosalicylidene)ethylenediaminato]copper(II) to Asp189 in the S1 pocket of trypsin is markedly different from of the amidino group of m-amidinosalicylidene-L-alaninato(aqua)copper(II) hydrochloride. The active site residues of trypsin play a crucial role in the binding affinity to the trypsin molecule.
-
additional information
-
inhinitory potencies of the different legume trypsin inhibitors, overview
-
additional information
-
aspartate (PepA) and cysteine (E64) protease inhibitors as well as the chymotrypsin inhibitor tosyl-L-phenylalanine chloromethylketone have no effect on enzymatic activity of isozyme TryIII
-
additional information
-
no inhibition by N-alpha-tosyl-L-Phe-chloromethyl ketone, E-64, and O-fenantroline
-
additional information
-
not inhibitory: chymostatin
-
additional information
-
the crude and partially purified extract from Passiflora f. edulis flavicarpa leaves displays inhibitory effect towards trypsin, methyl jasmonate treatment of the leaves increases the inhibitory activity against trypsin
-
additional information
-
no or poor inhibition by E-64, N-ethylmaleimide, iodoacetic acid, TPCK, pepstatin A, and EDTA
-
additional information
-
no inhibition by N-ethylmaleimide
-
additional information
no inhibition by N-ethylmaleimide
-
additional information
-
1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane, N-ethylmaleimide, iodoacetic acid, chymotrypsin, pepstatin A, and EDTA have no inhibitory effect
-
additional information
-
no or poor inhibition by E-64, N-ethylmaleimide, iodoacetic acid, TPCK, pepstatin A, and EDTA
-
additional information
-
trypsin 4 is completely resistant to chymotryptic degradation
-
additional information
-
mesotrypsin exhibits complete resistance against wild-type alpha1-antitrypsin
-
additional information
-
resistant to soybean trypsin inhibitor and aprotinin
-
additional information
-
trypsin activity decreases with age, trypsin activity correlates inversely with cholesterol concentration and plasmin and elastase activity
-
additional information
-
not inhibited by up to 0.05 mM SVIGCWTFSIPPRPCFVK-amide
-
additional information
-
identification of trypsin-3 binding peptides, and molecular modeling of the binding of peptides to isozyme trypsin-3. The degradation rate of peptides by trypsin correlates to their inhibitory efficacy, the most stable peptides being the most effective inhibitors, overview
-
additional information
-
determination of serum trypsin inhibitory capacity of plasma in psoriatic patients, which is increased compared to healthy persons
-
additional information
-
no or poor inhibition of trypsin A and trypsin B by pepstatin at 0.001 mM, and EDTA and 1,10-phenathroline at 10 mM
-
additional information
-
no or poor inhibition by N-tosyl-L-phenylalanine chloromethylketone, EDTA, 2-mercaptoethanol, pepstatin A, iodoacetic acid, and E-64
-
additional information
-
infection of Lutzomyia longipalpis with Leishmania mexicana reduces trypsin-like activity in the sand fly midgut, overview
-
additional information
-
no or poor inhibition by pepstatin A, EDTA, 2-mercaptoethanol, and N-p-tosyl-L-phenylalanine chloromethyl ketone
-
additional information
-
no inhibition by EDTA, E-64, and pepstatin A
-
additional information
-
not inhibitory: Ca2+ up to 500 mM
-
additional information
-
resistant to soybean trypsin inhibitor and aprotinin
-
additional information
-
effective inhibition of the catalytic activity by metal ions observed in trypsin R96H is caused by specific and reversible reorganization of the active site in the enzyme
-
additional information
-
notinhibited by pepstatin A
-
additional information
-
no inhibition by DTNB and 2-mercaptoethanol
-
additional information
-
not inhibitory: EDTA, tosyl-L-Phe chloromethyl ketone
-
additional information
-
no inhibition by pepstatin A, 2-mercaptoethanol and EDTA
-
additional information
-
not inhibited by 1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane, N-ethylmaleimide, iodoacetic acid, N-tosyl-L-phenyl-alanine-chloromethyl ketone, and pepstatin
-
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0.0018
2-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0073
2-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0037
2-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0029
2-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.008
2-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0038
2-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0091
2-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0018
2-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0037
2-aminobenzoyl-AGGRAAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.008
2-aminobenzoyl-AGGRDAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.018
2-aminobenzoyl-AGGREAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0041
2-aminobenzoyl-AGGRFAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0035
2-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0098
2-aminobenzoyl-AGGRHAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0059
2-aminobenzoyl-AGGRIAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.002
2-aminobenzoyl-AGGRKAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0036
2-aminobenzoyl-AGGRLAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0025
2-aminobenzoyl-AGGRMAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0062
2-aminobenzoyl-AGGRNAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0039
2-aminobenzoyl-AGGRQAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0085
2-aminobenzoyl-AGGRSAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0043
2-aminobenzoyl-AGGRTAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.004
2-aminobenzoyl-AGGRVAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.004
2-aminobenzoyl-AGGRYAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0005 - 0.017
2-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.04
2-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0018
2-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0013 - 0.012
2-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0012 - 0.017
2-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0004 - 0.024
2-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.011
2-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0051
2-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0017 - 0.01
2-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0025
2-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0018 - 0.0023
2-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0022
2-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0017
2-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0012
2-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0025
2-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0018
2-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0024
2-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0031
2-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0024
2-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0034
2-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0017
2-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0024
2-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0069
Abz-ERFK-EDDnp
-
pH 8.0, 25°C
0.0005
Abz-FRFK-EDDnp
-
pH 8.0, 25°C
0.0163
Abz-GRFK-EDDnp
-
pH 8.0, 25°C
0.0068
Abz-HRFK-EDDnp
-
pH 8.0, 25°C
0.0006
Abz-KRFK-EDDnp
-
pH 8.0, 25°C
0.001
Abz-LRFK-EDDnp
-
pH 8.0, 25°C
0.0074
Abz-NRFK-EDDnp
-
pH 8.0, 25°C
0.0089
Abz-QRFK-EDDnp
-
pH 8.0, 25°C
0.0081
Abz-RDFK-EDDnp
-
pH 8.0, 25°C
0.0129
Abz-REFK-EDDnp
-
pH 8.0, 25°C
0.0159
Abz-RGFK-EDDnp
-
pH 8.0, 25°C
0.00185
Abz-RHFK-EDDnp
-
pH 8.0, 25°C
0.0049
Abz-RNFK-EDDnp
-
pH 8.0, 25°C
0.0031
Abz-RPFK-EDDnp
-
pH 8.0, 25°C
0.0074
Abz-RQFK-EDDnp
-
pH 8.0, 25°C
0.0017
Abz-RRFK-EDDnp
-
pH 8.0, 25°C
0.001
Abz-RSFK-EDDnp
-
pH 8.0, 25°C
0.00325
Abz-RTFK-EDDnp
-
pH 8.0, 25°C
0.0183
Abz-RVFK-EDDnp
-
pH 8.0, 25°C
0.0017
Abz-RYFK-EDDnp
-
pH 8.0, 25°C
0.0029
Abz-VRFK-EDDnp
-
pH 8.0, 25°C
0.0033
Abz-WRFK-EDDnp
-
pH 8.0, 25°C
0.0056
Abz-YRFK-EDDnp
-
pH 8.0, 25°C
0.242
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg(NO2)-4-nitroanilide
-
-
0.015
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg-4-nitroanilide
-
-
0.236
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Har-4-nitroanilide
-
-
0.017
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Lys-4-nitroanilide
-
-
0.361
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-CN)-4-nitroanilide
-
-
0.384
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-NH2)-4-nitroanilide
-
-
0.328
alpha-N-p-tosyl-L-arginine methyl ester
-
pH 8.5, 60°C
0.033 - 0.037
benzoyl-Arg 4-nitroanilide
0.0026 - 0.09
benzoyl-Arg ethyl ester
0.034 - 0.43
benzoyl-Arg-p-nitroanilide
0.067 - 0.16
benzoyl-DL-Arg-4-nitroanilide
0.07744
benzoyl-DL-Arg-7-amido-4-methylcoumarin
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
3
benzoyl-DL-Arg-p-nitroanilide
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
0.02 - 0.44
benzoyl-L-arginine-p-nitroanilide
0.00049
benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
0.00064
benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
0.0141 - 0.0182
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
0.017
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide
-
at 25°C
0.00038 - 0.00054
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
0.00028 - 0.00036
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
0.00039 - 0.00082
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
0.0007 - 0.00112
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
0.0003 - 0.00053
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
0.00058 - 0.00059
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
0.00059
benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
0.00092
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
0.0285 - 0.125
Benzyloxycarbonyl-Lys methyl ester
0.01 - 1.6
benzyloxycarbonyl-Lys p-nitrophenyl ester
0.086 - 2.1
Benzyloxycarbonyl-Lys-thiobenzyl ester
0.00102
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
0.0061 - 0.0177
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
0.24
bovine serum albumin
-
-
-
0.00399
enhanced green fluorescent protein-T1
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
-
0.00421
enhanced green fluorescent protein-T1nb
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
-
0.0089 - 0.285
factor Xa
-
0.00015 - 0.00354
FITC-casein
-
0.1115
GERGFFYTPKT
-
pH 8.0, 37°C
0.0092
GERGFFYTPKTRR
-
pH 8.0, 37°C
0.485
Glu-Gly-Arg-4-nitroanilide
-
pH 7.4, 25°C
0.115
human M-proinsulin
-
pH 8.0, 37°C
-
0.12
Insulin
-
pH 8.0, 37°C
-
0.03
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
0.096
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide
-
-
0.044
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
0.24
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide
-
-
0.0085
M-diarginyl insulin
-
pH 8.0, 37°C
-
0.12
M-insulin
-
pH 8.0, 37°C
-
0.027
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25°C
0.013
MeOSuc-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25°C
0.018
MeOSuc-Ala-Arg 4-nitroanilide
-
pH 8.0, 25°C
32 - 35.5
N-alpha-benzoyl-L-Arg ethyl ester
0.55 - 7.06
N-alpha-benzoyl-L-Arg-p-nitroanilide
2.7 - 3.2
N-alpha-benzoyl-L-arginine 4-nitroanilide
1.26 - 3.2
N-alpha-benzoyl-L-arginine ethyl ester
0.06 - 0.09
N-alpha-benzoyl-L-arginine-p-nitroanilide
0.68 - 5.7
N-alpha-benzoyl-L-Lys-4-nitroanilide
0.2 - 0.33
N-alpha-p-tosyl-L-arginine methyl ester
0.158
N-benzoyl-DL-Arg-4-nitroanilide
-
-
0.05 - 0.507
N-benzoyl-DL-arginine 4-nitroanilide
0.0077
N-benzoyl-L-Arg ethyl ester
-
-
0.88
N-benzoyl-L-arginine-p-nitroanilide
-
in 67 mM sodium phosphate buffer, pH 7.6, at 25°C
0.007 - 0.034
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
0.08 - 22
N-benzyloxycarbonyl-L-lysine benzyl ester
0.0135 - 0.0274
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
0.15 - 0.156
Nalpha-4-tosyl-L-arginine methyl ester
0.077 - 1.12
Nalpha-benzoyl-DL-Arg-p-nitroanilide
0.068
Nalpha-benzoyl-DL-arginine 4-nitroanilide
-
pH 10.5, 25°C
0.083 - 0.125
Nalpha-benzoyl-DL-arginine-4-nitroanilide
0.051
Nalpha-benzoyl-L-Arg-4-nitroanilide
-
pH 8.0, 25°C
0.0153
Nalpha-benzoyl-L-arginine ethyl ester
-
pH 8.0, 37°C
0.0012 - 0.168
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
0.25
Nalpha-p-tosyl-L-Arg methyl ester
-
pH 8.0, 30°C
0.0013 - 0.045
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0057
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0033 - 0.017
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.002 - 0.0071
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0025 - 0.044
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.016 - 0.036
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0053 - 0.023
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0031 - 0.019
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.01 - 0.0233
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0031 - 0.015
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.015
o-aminobenzoyl-AGGRGDGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.013
o-aminobenzoyl-AGGRGEGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.009
o-aminobenzoyl-AGGRGFGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0024
o-aminobenzoyl-AGGRGGGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0032
o-aminobenzoyl-AGGRGHGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.003
o-aminobenzoyl-AGGRGIGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0009
o-aminobenzoyl-AGGRGKGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.002
o-aminobenzoyl-AGGRGLGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0024
o-aminobenzoyl-AGGRGMGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0037
o-aminobenzoyl-AGGRGNGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0028
o-aminobenzoyl-AGGRGQGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0029
o-aminobenzoyl-AGGRGSGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.007
o-aminobenzoyl-AGGRGTGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0027
o-aminobenzoyl-AGGRGVGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.006
o-aminobenzoyl-AGGRGYGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0083
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0031 - 0.029
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0053 - 0.018
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.012
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.015
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.017
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0033 - 0.025
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0011 - 0.027
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0163
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0014 - 0.019
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.011 - 0.013
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0013
o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0015 - 0.024
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.001 - 0.0079
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0013 - 0.016
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0008 - 0.016
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0018 - 0.0064
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0016 - 0.013
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0017 - 0.019
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0041
o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0015 - 0.037
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0013 - 0.015
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0387
p-amidinophenyl acetate
-
-
21
p-nitrophenyl acetate
-
-
0.029
p-tosyl-L-Arg methyl ester
-
-
0.023 - 0.0844
Pefachrome
0.013 - 0.0472
Pefachrome tPA
0.00167 - 0.07
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
0.033 - 0.099
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
0.00217 - 0.31
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
0.004 - 0.1
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
0.101 - 0.839
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
0.004 - 0.78
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
0.000167
succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
0.08 - 0.2
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
0.00927
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
0.0305
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 30°C
0.01 - 0.05
Tos-L-Arg-OMe
0.017 - 6.2
tosyl-Arg methyl ester
0.00017 - 0.000896
tosyl-Gly-Pro-Arg-4-nitroanilide
0.002 - 0.231
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
0.0141 - 0.346
Z-Gly-Pro-Arg-p-nitroanilide
additional information
additional information
-
0.0005
2-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0006
2-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.017
2-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0013
2-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0017
2-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.012
2-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0012
2-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0024
2-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.017
2-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0004
2-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0013
2-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.024
2-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0017
2-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.01
2-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0018
2-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
0.0023
2-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.033
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25°C, isoenzyme aT-II
0.037
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25°C, isoenzyme aT-I
0.0026
benzoyl-Arg ethyl ester
-
alpha-trypsin
0.014
benzoyl-Arg ethyl ester
-
pH 8.0, 25°C, isoenzyme aT-II
0.031
benzoyl-Arg ethyl ester
-
pH 8.0, 25°C, isoenzyme aT-I
0.09
benzoyl-Arg ethyl ester
-
gamma-trypsin
0.034
benzoyl-Arg-p-nitroanilide
-
at 25°C
0.43
benzoyl-Arg-p-nitroanilide
-
at 25°C
0.067
benzoyl-DL-Arg-4-nitroanilide
-
pH 7.6, 25°C
0.13
benzoyl-DL-Arg-4-nitroanilide
-
pH 8.0, 25°C
0.16
benzoyl-DL-Arg-4-nitroanilide
-
pH 8.0, 50°C
0.02
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 5°C
0.02
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 5°C
0.02
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 15°C
0.02
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 25°C
0.02
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 5°C
0.03
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 15°C
0.03
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 25°C
0.03
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 15°C
0.04
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 25°C
0.04
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 35°C
0.05
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 35°C
0.07
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 35°C
0.2
benzoyl-L-arginine-p-nitroanilide
-
at 5°C
0.26
benzoyl-L-arginine-p-nitroanilide
-
at 25°C
0.27
benzoyl-L-arginine-p-nitroanilide
-
at 15°C
0.44
benzoyl-L-arginine-p-nitroanilide
-
at 35°C
0.0141
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
wild-type enzyme
0.0162
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme E151Q
0.0177
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme N143H
0.0182
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme E151H
0.00038
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
0.00054
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
0.00028
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
0.00036
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
0.00039
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
0.00082
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
0.0007
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
0.00112
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
0.0003
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
0.00053
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
0.00058
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
0.00059
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
0.0285
Benzyloxycarbonyl-Lys methyl ester
-
gamma-trypsin
0.125
Benzyloxycarbonyl-Lys methyl ester
-
alpha-trypsin
0.01
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
alpha-trypsin
1.6
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
gamma-trypsin
0.086
Benzyloxycarbonyl-Lys-thiobenzyl ester
-
wild-type enzyme
2.1
Benzyloxycarbonyl-Lys-thiobenzyl ester
-
mutant D189H
0.0061
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37°C
0.012
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y/Q72R, pH 8.0, 37°C
0.0172
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y, pH 8.0, 37°C
0.0175
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant Y71L, pH 8.0, 37°C
0.0177
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37°C
0.0089
factor Xa
wild-type, pH 8.0, 25°C
-
0.0205
factor Xa
mutant Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C
-
0.0222
factor Xa
mutant G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C
-
0.285
factor Xa
mutant Y172S/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C
-
0.00015
FITC-casein
-
pH 7.0, 37°C
-
0.00016
FITC-casein
-
pH 8.6, 37°C
-
0.000209
FITC-casein
-
pH 7.5, 37°C
-
0.000215
FITC-casein
-
pH 8.1, 37°C
-
0.00086
FITC-casein
-
pH 7.8, 30°C
-
0.00298
FITC-casein
-
pH 7.8, 58°C
-
0.00333
FITC-casein
-
pH 7.8, 37°C
-
0.00354
FITC-casein
-
pH 7.8, 48°C
-
32
N-alpha-benzoyl-L-Arg ethyl ester
-
enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, pH 8.0, 25°C
35.5
N-alpha-benzoyl-L-Arg ethyl ester
-
wild-type, pH 8.0, 25°C
0.55
N-alpha-benzoyl-L-Arg-p-nitroanilide
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
7.06
N-alpha-benzoyl-L-Arg-p-nitroanilide
-
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
2.7
N-alpha-benzoyl-L-arginine 4-nitroanilide
-
pH 8.3, 25°C, recombinant enzyme from Zea may
3.2
N-alpha-benzoyl-L-arginine 4-nitroanilide
-
pH 8.3, 25°C, native bovine trypsin
1.26
N-alpha-benzoyl-L-arginine ethyl ester
-
in 20 mM TBS (pH 7.6) for 10 min at 25°C, in the absence of sugars or glycoproteins
2.81
N-alpha-benzoyl-L-arginine ethyl ester
-
in 20 mM TBS (pH 7.6) for 10 min at 25°C, in the presence of 0.2 M methyl-alpha-D-galactoside
3.2
N-alpha-benzoyl-L-arginine ethyl ester
-
in 20 mM TBS (pH 7.6) for 10 min at 25°C, in the presence of 0.2 M methyl-alpha-D-mannoside
0.06
N-alpha-benzoyl-L-arginine-p-nitroanilide
-
isozyme TLE II, at 37°C, pH 8.0
0.09
N-alpha-benzoyl-L-arginine-p-nitroanilide
-
isozyme TLE I, at 37°C, pH 8.0
0.68
N-alpha-benzoyl-L-Lys-4-nitroanilide
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
5.7
N-alpha-benzoyl-L-Lys-4-nitroanilide
-
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
0.2
N-alpha-p-tosyl-L-arginine methyl ester
-
trypsin B, at pH 8.0 and 30°C
0.33
N-alpha-p-tosyl-L-arginine methyl ester
-
trypsin A, at pH 8.0 and 30°C
0.05
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25°C
0.064
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25°C
0.13
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 9.0, 25°C
0.387
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.5, 25°C
0.507
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.5, 60°C
0.007
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 8.0, 25°C
0.009
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 9.0, 25°C
0.015
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 7.1, 25°C
0.034
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 6.0, 25°C
0.08
N-benzyloxycarbonyl-L-lysine benzyl ester
-
alpha-trypsin
22
N-benzyloxycarbonyl-L-lysine benzyl ester
-
gamma-trypsin
0.0135
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
0.0198
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193A, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
0.0213
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193Y, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
0.0252
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
0.0274
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193F, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
0.15
Nalpha-4-tosyl-L-arginine methyl ester
-
pH 8.5, 45°C
0.156
Nalpha-4-tosyl-L-arginine methyl ester
-
pH 8.5, 25°C
0.077
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
-
0.197
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
cationic trypsin 1
0.244
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
cationic trypsin 2
0.619
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
anionic trypsin
0.99
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
anionic enzyme
1.12
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
anionic enzyme
0.083
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin B, pH 9.0, 25°C
0.1
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin C, pH 9.0, 25°C
0.125
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin A, pH 9.0, 25°C
0.0012
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
without addition of methanol
0.0038
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 10% methanol
0.0096
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 20% methanol
0.0176
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 30% methanol
0.168
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 50% methanol
0.0013
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.005
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.045
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0033
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.01
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.017
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.002
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0071
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0025
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.027
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.044
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.016
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.019
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.036
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0053
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.022
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.023
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0031
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.009
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.019
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.01
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.011
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.016
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0233
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0031
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.006
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0074
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.015
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0031
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.015
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.029
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0053
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.009
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.018
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0033
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.015
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.025
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0011
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.023
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.027
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0014
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.018
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.019
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.011
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.013
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0015
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0032
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.024
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.001
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0079
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0013
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.008
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.016
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0008
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.014
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.016
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0018
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0064
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0016
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.01
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.013
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0017
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.019
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0015
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.013
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.037
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0013
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.015
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.023
Pefachrome
-
pH 8.0, wild-type enzyme
0.0342
Pefachrome
-
pH 8.0, mutant enzyme E97N/Y99L
0.0443
Pefachrome
-
pH 8.0, mutant enzyme S190A
0.0844
Pefachrome
-
pH 8.0, mutant enzyme E97N/Y99L/S190A
0.013
Pefachrome tPA
-
native enzyme
0.0229
Pefachrome tPA
-
variant X99rT containing the 99-loop (Glu97,Tyr99) of factor Xa
0.0472
Pefachrome tPA
-
variant X(99/175/190)rT containing the 99-loop the 175-loop (Ser172,Ser173, Phe174, Ile175) and Ala190 of factor Xa
0.00167
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
0.07
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
0.033
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 8
0.057
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 9
0.087
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 7
0.099
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 10
0.00217
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
0.31
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
0.004
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
0.1
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
0.101
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 9
0.105
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 8
0.184
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 10
0.839
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 7
0.004
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
0.78
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
0.08
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
0.2
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
0.01
Tos-L-Arg-OMe
-
at 25°C
0.01
Tos-L-Arg-OMe
-
at 5°C
0.01
Tos-L-Arg-OMe
-
at 15°C
0.02
Tos-L-Arg-OMe
-
at 35°C
0.02
Tos-L-Arg-OMe
-
isozyme ST-1, at 5°C
0.02
Tos-L-Arg-OMe
-
isozyme ST-2, at 15°C
0.02
Tos-L-Arg-OMe
-
isozyme ST-2, at 25°C
0.02
Tos-L-Arg-OMe
-
isozyme ST-2, at 5°C
0.02
Tos-L-Arg-OMe
-
isozyme ST-3, at 25°C
0.02
Tos-L-Arg-OMe
-
isozyme ST-3, at 5°C
0.03
Tos-L-Arg-OMe
-
isozyme ST-1, at 15°C
0.03
Tos-L-Arg-OMe
-
isozyme ST-2, at 35°C
0.03
Tos-L-Arg-OMe
-
isozyme ST-3, at 15°C
0.04
Tos-L-Arg-OMe
-
isozyme ST-1, at 25°C
0.04
Tos-L-Arg-OMe
-
isozyme ST-3, at 35°C
0.05
Tos-L-Arg-OMe
-
isozyme ST-1, at 35°C
0.017
tosyl-Arg methyl ester
-
alpha-trypsin
0.06
tosyl-Arg methyl ester
-
gamma-trypsin
0.12
tosyl-Arg methyl ester
Cambarus affinis
-
free enzyme
1.53
tosyl-Arg methyl ester
Cambarus affinis
-
enzyme immobilized on Sepharose 4B-KT
2.5
tosyl-Arg methyl ester
Cambarus affinis
-
enzyme immobilized on porous glass loaded with isothiocyanate
6.2
tosyl-Arg methyl ester
Cambarus affinis
-
enzyme immobilized on Sepharose 4B-KT
0.00017
tosyl-Gly-Pro-Arg-4-nitroanilide
-
pH 8.3
0.000896
tosyl-Gly-Pro-Arg-4-nitroanilide
salmon
-
pH 8.3
0.002
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, recombinant enzyme
0.0023
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, wild-type enzyme
0.0032
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, recombinant enzyme
0.0036
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, wild-type enzyme
0.0044
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190A
0.0061
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190S
0.0101
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190S
0.0129
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190A
0.067
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190P
0.166
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190P
0.224
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190V
0.231
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190V
0.0141
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
0.0203
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
0.0266
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
0.0298
Z-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
0.0788
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
0.346
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
influence of pH-value on Km-value of wild-type and mutant enzymes
-
additional information
additional information
-
the mutations C191A and C220A decrease the activity of trypsin by 20-200fold as measured by the ratio of turnover number to Km-value for the hydrolysis of amide substrates, ester hydrolysis is decreased by less than 10fold
-
additional information
additional information
-
peptide synthesis using 4-amidino- and 4-guanidinophenyl esters of Nalpha-tert-butyloxycarbonyl-amino acid as acyl donor components with two types of acyl acceptors, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
33000
2-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
3800
2-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
13300
2-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
30000
2-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
330000
2-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
240000
2-aminobenzoyl-AGGRAAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
123000
2-aminobenzoyl-AGGRDAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
133000
2-aminobenzoyl-AGGREAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
217000
2-aminobenzoyl-AGGRFAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
200000
2-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
183000
2-aminobenzoyl-AGGRHAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
133000
2-aminobenzoyl-AGGRIAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
230000
2-aminobenzoyl-AGGRKAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
138000
2-aminobenzoyl-AGGRLAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
200000
2-aminobenzoyl-AGGRMAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
300000
2-aminobenzoyl-AGGRNAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
230000
2-aminobenzoyl-AGGRQAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
400000
2-aminobenzoyl-AGGRSAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
200000
2-aminobenzoyl-AGGRTAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
133000
2-aminobenzoyl-AGGRVAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
167000
2-aminobenzoyl-AGGRYAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
630000
2-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
117000
2-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
130000
2-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
400000
2-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
910000
2-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
410000
2-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
26700
2-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
20000
2-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
41000
2-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
33000
2-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
41000
2-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
16300
2-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
50000
2-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
31700
2-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
16500
2-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
21700
2-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
31700
2-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
pH 8.5, 30°C
17.3
Abz-ERFK-EDDnp
-
pH 8.0, 25°C
8.87
Abz-FRFK-EDDnp
-
pH 8.0, 25°C
31.94
Abz-GRFK-EDDnp
-
pH 8.0, 25°C
21.07
Abz-HRFK-EDDnp
-
pH 8.0, 25°C
53.23
Abz-KRFK-EDDnp
-
pH 8.0, 25°C
53.23
Abz-LRFK-EDDnp
-
pH 8.0, 25°C
30.61
Abz-NRFK-EDDnp
-
pH 8.0, 25°C
12.64
Abz-QRFK-EDDnp
-
pH 8.0, 25°C
2.33
Abz-RDFK-EDDnp
-
pH 8.0, 25°C
7.49
Abz-REFK-EDDnp
-
pH 8.0, 25°C
9.15
Abz-RGFK-EDDnp
-
pH 8.0, 25°C
8.32
Abz-RHFK-EDDnp
-
pH 8.0, 25°C
16.63
Abz-RNFK-EDDnp
-
pH 8.0, 25°C
0.1
Abz-RPFK-EDDnp
-
pH 8.0, 25°C
13.31
Abz-RQFK-EDDnp
-
pH 8.0, 25°C
26.61
Abz-RRFK-EDDnp
-
pH 8.0, 25°C
16.63
Abz-RSFK-EDDnp
-
pH 8.0, 25°C
18.3
Abz-RTFK-EDDnp
-
pH 8.0, 25°C
4.59
Abz-RVFK-EDDnp
-
pH 8.0, 25°C
10.65
Abz-RYFK-EDDnp
-
pH 8.0, 25°C
46.57
Abz-VRFK-EDDnp
-
pH 8.0, 25°C
6.32
Abz-WRFK-EDDnp
-
pH 8.0, 25°C
27.72
Abz-YRFK-EDDnp
-
pH 8.0, 25°C
1.4
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg(NO2)-4-nitroanilide
-
-
23
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg-4-nitroanilide
-
-
19
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Lys-4-nitroanilide
-
-
3.2
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-N-omega-hydroxy-L-arginine-4-nitroanilide
-
-
0.7
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-CN)-4-nitroanilide
-
-
5.1
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-NH2)-4-nitroanilide
-
-
112
alpha-N-benzyloxycarbonyl-Lys-thiobenzyl ester
-
wild-type enzyme
112
alpha-N-p-tosyl-L-arginine methyl ester
-
pH 8.5, 60°C
1.94 - 3.7
benzoyl-Arg 4-nitroanilide
0.24 - 42.9
benzoyl-Arg ethyl ester
1.13 - 3.44
benzoyl-Arg-p-nitroanilide
1.46 - 23.8
benzoyl-DL-Arg-4-nitroanilide
0.25
benzoyl-DL-Arg-7-amido-4-methylcoumarin
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
1.33
benzoyl-DL-Arg-p-nitroanilide
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
0.3 - 7.7
benzoyl-L-arginine-p-nitroanilide
76
benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
92
benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
44.5 - 69.3
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
0.01 - 172.8
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide
41.14 - 42.99
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
4.65 - 14.85
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
5.42 - 12.19
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
67.79 - 72.08
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
3.2 - 8.07
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
5.51 - 25.75
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
72
benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
80
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
0.71 - 80
Benzyloxycarbonyl-Lys methyl ester
23 - 82
benzyloxycarbonyl-Lys p-nitrophenyl ester
12.3
Benzyloxycarbonyl-Lys-thiobenzyl ester
-
mutant D189H
148
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C
33 - 279.5
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
0.26
enhanced green fluorescent protein-T1
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
-
0.85
enhanced green fluorescent protein-T1nb
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
-
0.32 - 0.48
FITC-casein
-
0.313
GERGFFYTPKT
-
pH 8.0, 37°C
4.15
GERGFFYTPKTRR
-
pH 8.0, 37°C
11.65
human M-proinsulin
-
pH 8.0, 37°C
-
0.14
Insulin
-
pH 8.0, 37°C
-
3.2
L-Phe-L-Val-L-Ile-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
4.4
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
27.4
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide
-
-
4
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide
-
-
1.1
M-diarginyl insulin
-
pH 8.0, 37°C
-
0.14
M-insulin
-
pH 8.0, 37°C
-
30.5
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25°C
33.6
MeOSuc-Ala-Ala-Arg 4-nitroanilide
-
pH 8.0, 25°C
24.9
MeOSuc-Ala-Arg 4-nitroanilide
-
pH 8.0, 25°C
10.5 - 12.3
N-alpha-benzoyl-L-Arg ethyl ester
2.46 - 5.73
N-alpha-benzoyl-L-Arg-p-nitroanilide
0.84 - 0.98
N-alpha-benzoyl-L-arginine-p-nitroanilide
0.56 - 1.04
N-alpha-benzoyl-L-Lys-4-nitroanilide
66.7 - 80
N-alpha-p-tosyl-L-arginine methyl ester
0.8
N-benzoyl-DL-Arg-4-nitroanilide
-
-
1.34 - 4.71
N-benzoyl-DL-arginine 4-nitroanilide
3.8
N-benzoyl-DL-arginine-4-nitroanilide
-
6.1
N-benzoyl-L-arginine-p-nitroanilide
-
in 67 mM sodium phosphate buffer, pH 7.6, at 25°C
23.5 - 70.5
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide
18.3 - 54.5
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
8 - 84
N-benzyloxycarbonyl-L-lysine benzyl ester
110 - 162
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
59.15 - 210
Nalpha-4-tosyl-L-arginine methyl ester
0.355 - 1
Nalpha-benzoyl-DL-Arg-p-nitroanilide
2.76
Nalpha-benzoyl-DL-arginine 4-nitroanilide
-
pH 10.5, 25°C
1.21 - 5.76
Nalpha-benzoyl-DL-arginine-4-nitroanilide
2.12
Nalpha-benzoyl-L-Arg-4-nitroanilide
-
pH 8.0, 25°C
16.5
Nalpha-benzoyl-L-arginine ethyl ester
-
pH 8.0, 37°C
0.013 - 0.13
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
200
Nalpha-p-tosyl-L-Arg methyl ester
-
pH 8.0, 30°C
0.0067 - 1.2
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0014 - 0.013
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0045 - 0.08
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0072 - 1.2
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00022 - 52
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00004 - 5.9
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00004 - 1.4
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00022 - 26
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00004 - 630
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00011 - 820
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00014
o-aminobenzoyl-AGGRGDGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00013
o-aminobenzoyl-AGGRGEGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0034
o-aminobenzoyl-AGGRGFGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00029
o-aminobenzoyl-AGGRGGGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00063
o-aminobenzoyl-AGGRGHGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0007
o-aminobenzoyl-AGGRGIGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00043
o-aminobenzoyl-AGGRGKGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00068
o-aminobenzoyl-AGGRGLGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00063
o-aminobenzoyl-AGGRGMGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00027
o-aminobenzoyl-AGGRGNGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00015
o-aminobenzoyl-AGGRGQGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00034
o-aminobenzoyl-AGGRGSGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0007
o-aminobenzoyl-AGGRGTGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00078
o-aminobenzoyl-AGGRGVGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00145
o-aminobenzoyl-AGGRGYGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00027 - 14
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00022 - 12
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00072 - 41
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00017 - 19
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00015 - 17
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00017 - 140
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00014 - 30
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00011 - 14
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00011 - 0.13
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00015 - 26
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00022 - 11
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.004
o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0048 - 0.37
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0055 - 2.1
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0052 - 0.95
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.005 - 1.9
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0043 - 0.83
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.0068 - 1.4
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.005 - 0.013
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.006
o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0047 - 0.9
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.005 - 0.013
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
0.00167 - 91
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
95 - 163
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
0.000167 - 0.00217
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
0.004 - 121.2
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
40 - 74
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
0.004 - 0.0063
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
0.000167
succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
0.00067 - 0.08
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
1.41
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin
-
in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C
14.7
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 30°C
1.7 - 76
tosyl-Arg methyl ester
28 - 88
tosyl-Gly-Pro-Arg-4-nitroanilide
0.52 - 82.5
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
0.04 - 8.78
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin
3.86 - 88.2
Z-Gly-Pro-Arg-p-nitroanilide
additional information
additional information
-
1.94
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25°C, isoenzyme aT-I
2.94
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25°C, isoenzyme aT-I
3.7
benzoyl-Arg 4-nitroanilide
-
pH 8.0, 25°C, isoenzyme aT-II
0.24
benzoyl-Arg ethyl ester
-
gamma-trypsin
0.3
benzoyl-Arg ethyl ester
-
pH 8.0, 25°C, isoenzyme aT-II
26.6
benzoyl-Arg ethyl ester
-
alpha-trypsin
42.9
benzoyl-Arg ethyl ester
-
pH 8.0, 25°C, isoenzyme aT-I
1.13
benzoyl-Arg-p-nitroanilide
-
at 25°C
3.44
benzoyl-Arg-p-nitroanilide
-
at 25°C
1.46
benzoyl-DL-Arg-4-nitroanilide
-
pH 8.0, 25°C
6.17
benzoyl-DL-Arg-4-nitroanilide
-
pH 7.6, 25°C
23.8
benzoyl-DL-Arg-4-nitroanilide
-
pH 8.0, 50°C
0.3
benzoyl-L-arginine-p-nitroanilide
-
at 5°C
0.64
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 5°C
0.76
benzoyl-L-arginine-p-nitroanilide
-
at 15°C
0.83
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 5°C
0.94
benzoyl-L-arginine-p-nitroanilide
-
at 25°C
1.06
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 5°C
1.27
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 15°C
2
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 25°C
2.43
benzoyl-L-arginine-p-nitroanilide
-
at 35°C
2.72
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 15°C
2.74
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 15°C
3 - 6
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 15°C
3.13
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 25°C
3.86
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-3, at 35°C
4.06
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 25°C
5.54
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 25°C
6.74
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-2, at 35°C
7.7
benzoyl-L-arginine-p-nitroanilide
-
isozyme ST-1, at 35°C
44.5
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme N143H
49.3
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme E151Q
58.3
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
wild-type enzyme
69.3
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide
-
mutant enzyme E151H
0.01
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide
-
at 25°C
172.8
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide
-
at 25°C
41.14
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
42.99
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
4.65
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
14.85
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
5.42
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
12.19
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
67.79
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
72.08
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
3.2
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
8.07
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
5.51
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin A
25.75
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
pH 8.0, 37°C, trypsin B
0.71
Benzyloxycarbonyl-Lys methyl ester
-
gamma-trypsin
80
Benzyloxycarbonyl-Lys methyl ester
-
alpha-trypsin
23
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
gamma-trypsin
82
benzyloxycarbonyl-Lys p-nitrophenyl ester
-
alpha-trypsin
33
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37°C
51.3
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y, pH 8.0, 37°C
57.3
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant L71Y/Q72R, pH 8.0, 37°C
93
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
mutant Y71L, pH 8.0, 37°C
279.5
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide
-
wild-type enzyme, pH 8.0, 37°C
3
factor Xa
mutant Y172S/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C
-
9.25
factor Xa
mutant G186K/G187Q/V188E/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C
-
77.7
factor Xa
mutant Y172S/Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C
-
86.4
factor Xa
mutant Y217E/P222K/Y224K/P225Y, pH 8.0, 25°C
-
124
factor Xa
wild-type, pH 8.0, 25°C
-
0.32
FITC-casein
-
pH 7.0, 37°C
-
0.35
FITC-casein
-
pH 7.8, 30°C
-
0.4
FITC-casein
-
pH 7.5, 37°C
-
0.43
FITC-casein
-
pH 8.6, 37°C
-
0.44
FITC-casein
-
pH 7.8, 37°C
-
0.44
FITC-casein
-
pH 8.1, 37°C
-
0.48
FITC-casein
-
pH 7.8, 48°C
-
0.48
FITC-casein
-
pH 7.8, 58°C
-
10.5
N-alpha-benzoyl-L-Arg ethyl ester
-
enzyme with O-carboxymethyl-poly-beta-cyclodextrin attached to surface, pH 8.0, 25°C
12.3
N-alpha-benzoyl-L-Arg ethyl ester
-
wild-type, pH 8.0, 25°C
2.46
N-alpha-benzoyl-L-Arg-p-nitroanilide
-
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
5.73
N-alpha-benzoyl-L-Arg-p-nitroanilide
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
0.84
N-alpha-benzoyl-L-arginine-p-nitroanilide
-
isozyme TLE I, at 37°C, pH 8.0
0.98
N-alpha-benzoyl-L-arginine-p-nitroanilide
-
isozyme TLE II, at 37°C, pH 8.0
0.56
N-alpha-benzoyl-L-Lys-4-nitroanilide
-
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
1.04
N-alpha-benzoyl-L-Lys-4-nitroanilide
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
66.7
N-alpha-p-tosyl-L-arginine methyl ester
-
trypsin B, at pH 8.0 and 30°C
80
N-alpha-p-tosyl-L-arginine methyl ester
-
trypsin A, at pH 8.0 and 30°C
1.34
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25°C
1.56
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 9.0, 25°C
2.32
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.0, 25°C
2.62
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.5, 25°C
4.71
N-benzoyl-DL-arginine 4-nitroanilide
-
pH 8.5, 60°C
23.5
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide
-
isozyme trypsin-1, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2
32.7
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide
-
isozyme trypsin-2, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2
70.5
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide
-
isozyme trypsin-4, in 50 mM Tris, pH 8.0, 100 mM NaCl, 6 mM CaCl2
18.3
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 6.0, 25°C
48.9
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 9.0, 25°C
53.1
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 8.0, 25°C
54.5
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide
-
pH 7.1, 25°C
8
N-benzyloxycarbonyl-L-lysine benzyl ester
-
gamma-trypsin
84
N-benzyloxycarbonyl-L-lysine benzyl ester
-
alpha-trypsin
110
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
124
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193A, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
133
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193Y, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
158
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
162
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193F, in 50 mM Tricine, 10 mM CaCl2, pH 8.0 at 20°C
59.15
Nalpha-4-tosyl-L-arginine methyl ester
-
pH 8.5, 25°C
210
Nalpha-4-tosyl-L-arginine methyl ester
-
pH 8.5, 45°C
0.355
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
anionic trypsin
0.473
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
cationic trypsin 1
1
Nalpha-benzoyl-DL-Arg-p-nitroanilide
-
cationic trypsin 2
1.21
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin B, pH 9.0, 25°C
2.24
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin A, pH 9.0, 25°C
5.76
Nalpha-benzoyl-DL-arginine-4-nitroanilide
-
isozyme trypsin C, pH 9.0, 25°C
0.013
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
without addition of methanol
0.043
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 10% methanol
0.052
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 20% methanol
0.07
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 30% methanol
0.13
Nalpha-benzyloxycarbonyl-Lys-p-nitrophenyl ester
-
in presence of 50% methanol
0.0067
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.54
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
1.2
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0014
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.013
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0045
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.064
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.08
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0072
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
1.2
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00022
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.74
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
52
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00004
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.03
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
5.9
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00004
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.09
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
1.4
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00022
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.43
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
26
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00004
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
1.4
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
1.7
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
630
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00011
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.9
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
2.7
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
820
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00027
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.08
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
14
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00022
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.38
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
12
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00072
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.4
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
41
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00017
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.26
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
19
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00015
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.34
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
17
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00017
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.08
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
140
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00014
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.27
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
30
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00011
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.16
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
14
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00011
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.13
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00015
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.4
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
26
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00022
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.3
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
11
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0048
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.26
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.37
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0055
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.17
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
2.1
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0052
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.12
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.95
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.005
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.22
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
1.9
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0043
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.83
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0068
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.19
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
1.4
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.005
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.013
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.0047
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.57
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.9
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.005
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.013
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine)
-
at 30°C in 100 mM Tris-HCl buffer pH 8.5
0.00167
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
91
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
95
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 7
144
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 9
145
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 10
163
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide
-
wild-type enzyme, pH 8
0.000167
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
0.00217
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
0.004
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
73
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
121.2
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
40
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 7
59
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 9
64
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 10
74
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide
-
wild-type enzyme, pH 8
0.004
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
0.0063
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
0.00067
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, wild-type enzyme
0.08
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
-
pH 8.0, 37°C, mutant enzyme D189S/DELTA223
41.3
Tos-L-Arg-OMe
-
isozyme ST-1, at 5°C
46.2
Tos-L-Arg-OMe
-
at 5°C
61.7
Tos-L-Arg-OMe
-
isozyme ST-2, at 5°C
64.5
Tos-L-Arg-OMe
-
isozyme ST-1, at 15°C
68.4
Tos-L-Arg-OMe
-
isozyme ST-3, at 5°C
69.2
Tos-L-Arg-OMe
-
at 15°C
94.3
Tos-L-Arg-OMe
-
isozyme ST-1, at 25°C
94.3
Tos-L-Arg-OMe
-
isozyme ST-1, at 35°C
105
Tos-L-Arg-OMe
-
isozyme ST-2, at 15°C
109
Tos-L-Arg-OMe
-
at 25°C
139
Tos-L-Arg-OMe
-
at 35°C
154
Tos-L-Arg-OMe
-
isozyme ST-3, at 15°C
159
Tos-L-Arg-OMe
-
isozyme ST-3, at 25°C
189
Tos-L-Arg-OMe
-
isozyme ST-3, at 35°C
200
Tos-L-Arg-OMe
-
isozyme ST-2, at 35°C
234
Tos-L-Arg-OMe
-
isozyme ST-2, at 25°C
1.7
tosyl-Arg methyl ester
-
gamma-trypsin
76
tosyl-Arg methyl ester
-
alpha-trypsin
28
tosyl-Gly-Pro-Arg-4-nitroanilide
salmon
-
pH 8.3
30.4
tosyl-Gly-Pro-Arg-4-nitroanilide
salmon
-
pH 8.3
88
tosyl-Gly-Pro-Arg-4-nitroanilide
-
pH 8.3
0.52 - 0.7
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, wild-type enzyme
2.7
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, recombinant enzyme
8
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190P
38.33
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190V
82.5
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190A
0.04 - 1.97
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190P
0.5
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190V
3.2
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190A
8.78
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin
pH 7.6, mutant enzyme T190P
3.86
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G142A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
20 - 50
Z-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
29.2
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G184A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
51.5
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G/G19A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
54.1
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193G, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
83.6
Z-Gly-Pro-Arg-p-nitroanilide
-
mutant enzyme R193A, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
88.2
Z-Gly-Pro-Arg-p-nitroanilide
-
wild type enzyme, in 20 mM Tricine,10 mM CaCl2, at pH 8.0 and 20°C
additional information
additional information
-
the mutations C191A and C220A decrease the activity of trypsin by 20-200fold as measured by the ratio of turnover number to Km-value for the hydrolysis of amide substrates, ester hydrolysis is decreased by less than 10fold
-
additional information
additional information
-
influence of pH-value on turnover number of wild-type and mutant enzymes
-
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