crystallization under sulfate-free conditions containing 0.3 M NaCl and 50 mM tris(hydroxymethyl)aminomethane-HCl at pH 7.0. Crystal structure determined at 1.5 A resolution has a unique conformation in four regions which contains loop portions
in complex with the inhibitor 4-([(S)-1-[((S)-2-[[(RS)-3,3,3-trifluoro-1-isopropyl-2-oxopropyl]aminocarbonyl]pyrrolidin-1-yl-)carbonyl]-2-methylpropyl]aminocarbonyl)benzoic acid, sitting drop vapour diffusion method using 50 mM D-substituted sodium acetate and 0.2-0.3 M sodium sulfate
the squash inhibitor MCE III and the third domain of turkey ovomucoid inhibitor OMTKY3 are crystallized in complexes with porcine pancreatic elastase. Crystals of the complex between MCEI III and pancreatic elastase are grown in citrate buffer with and without ammonium acetate. X-ray diffraction data are collected to 1.9 A resolution at room temperature using synchrotron radiation. The crystals belong to space group P21, with unit-cell parameters a = 49.17 A, beta = 44.59 A, c = 67.08 A, beta = 110-97°. Crystals of the OMTKY3/pancreatic elastase complex are obtained in the presence of ammonium sulfate, MES buffer and polyethylene glycol monomethylether. The crystrals of this complex diffract to 2.1 A resolution and belong to space group I222, with unit-cell parameters a = 84.58, b = 84.61, c = 89.92 A
when the native structure is achieved, cosolvents increase the stability of the enzyme to the same extent as does the acylation of the active center residue Ser195. Dimethylsulfoxide, glycerol and methanol enhance the stability of the intermediates able to refold into the native form, contrary to acetonitrile
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renatured by dilution in the solution of substrate at various pHs under agitation. A lag period is observed before reaching the steady state of the hydrolysis of an amide substrate, and the lag period measured with the refolding enzyme is longer than that measured with the native elastase
intratracheal administration of two doses of ELT induces a proinflammatory response in the lung that is characterized by significant infiltration of macrophages and an increased level of interleukin-1beta in lung homogenates
type I pancreatic elastase PRT-201 treatment of atherosclerotic peripheral arteries in patients can increase artery diameter, and thus luminal area, possibly alleviating some of the symptoms of peripheral artery disease