a binuclear zinc center is located in each subunit at the C-terminal end of the (beta,alpha)8-barrel. Ligands to the binuclear metal center include His68, His70, His201, His230, Asp285. The two zincs are bridged by a carboxylated lysine residue Lys162 and a solvent molecule
inactive precursor undergoes a self-activation process, converting it from an uncleaved precursor of 35000 Da into alpha- and beta-subunits of 20000 and 15000 Da, respectively. Glycine stimulates cleavage in a dose-dependent manner
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
uncleaved (inactive) as well as the cleaved (active), aspartate-bound structures. Cleavage does not greatly alter the overall structure of the enzyme with the exception of the conformational change in the conserved HGG loop that coincides with cleavage
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS