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IUBMB Comments Inactivates vasopressin and oxytocin by splitting off glycinamide. Also cleaves ester substrates of trypsin and chymotrypsin. Although glycinamide is by far the preferred leaving group, other aminoacylamides may also be released, e.g. phenylalaninamide. The toad skin enzyme is inhibited by diisopropyl fluorophosphate.
Reaction Schemes
cleavage of C-terminal glycinamide from polypeptides
Synonyms
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EC 3.4.15.2
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formerly
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glycinamidase, peptidyl
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peptidyl amino acid amide hydrolase
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peptidyl carboxy-amidase
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peptidyl carboxyamidase
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peptidyl-aminoacylamidase
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peptidyl-glycinamidase
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carboxamidopeptidase
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cleavage of C-terminal glycinamide from polypeptides
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hydrolysis of peptide bond
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acetyl-L-Trp ethyl ester + H2O
?
toad
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Substrates: -
?
alpha-aminosuberic acid + H2O
?
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Substrates: -
?
alpha-aminosuberic acid oxytocin + H2O
glycinamide + ?
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Substrates: -
?
alpha-deamino-cystathionine + H2O
?
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Substrates: -
?
alpha-deamino-cystathionine-6,1-oxytocin + H2O
glycinamide + ?
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Substrates: -
?
antidiuretic hormone + H2O
glycinamide + ?
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Substrates: release of glycinamide from the C-terminus of the hormone
?
benzoyl-L-Arg ethyl ester + H2O
?
toad
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Substrates: -
?
beta-deamino-cystathionine-1,6-oxytocin + H2O
glycinamide + ?
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Substrates: -
?
oxytocin + H2O
?
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Substrates: the enzyme is not a strict peptidyl-glycinamidase, but can release other amino acid amides from a variety of amidated peptide
?
oxytocin + H2O
glycinamide + ?
Vasopressin + H2O
?
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Substrates: -
?
[Arg8]-vasopressin + H2O
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toad
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Substrates: hydrolysis of the Arg8-Gly9-NH2 bond
?
additional information
?
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toad
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Substrates: enzyme inactivates neurophyseal hormones
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oxytocin + H2O
glycinamide + ?
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Substrates: -
?
oxytocin + H2O
glycinamide + ?
toad
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Substrates: hydrolysis of the Leu8-Gly9-NH2 bond
?
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additional information
?
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toad
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Substrates: enzyme inactivates neurophyseal hormones
?
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4-methylumbelliferyl p-guanidinobenzoate
toad
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alpha-aminosuberic acid oxytocin
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at high concentrations
beta-deamino-cystathionine-1,6-oxytocin
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at high concentrations
concanavalin A
toad
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diisopropyl phosphofluoridate
Lima bean trypsin inhibitor
toad
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p-Nitrophenyl p-guanidinobenzoate
toad
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tosyllysine chloromethyl ketone
toad
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tosylphenylalanine chloromethyl ketone
toad
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diisopropyl phosphofluoridate
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diisopropyl phosphofluoridate
toad
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PCMB
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PCMB
toad
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reversed by Cys
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0.0025 - 0.0033
alpha-aminosuberic acid
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0.0025 - 0.0033
alpha-deamino-cystathionine
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0.00625
beta-deamino-cystathionine-1,6-oxytocin
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0.0025 - 0.0033
Oxytocin
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7.5 - 8.5
toad
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hydrolysis of 8-arginine-vasopressin or benzoyl-L-Arg ethyl ester
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brenda
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brenda
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brenda
toad
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brenda
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medulla
brenda
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brenda
toad
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brenda
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brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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100000
toad
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gel filtration
48000
toad
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2 * 48000, each subunit consists of a heavy chain, 28000 da, and a light chain, 19000 Da joined by a disulfide bond, SDS-PAGE
48000
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2 * 48000, each subunit consists of two polypeptide chains of 28 kDa and 19 kDa linked via two disulfide bonds
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dimer
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2 * 48000, each subunit consists of two polypeptide chains of 28 kDa and 19 kDa linked via two disulfide bonds
dimer
toad
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2 * 48000, each subunit consists of a heavy chain, 28000 da, and a light chain, 19000 Da joined by a disulfide bond, SDS-PAGE
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EDTA, 0.001 mM, stabilizes during purification
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Nardacci, N.J.; Mukhopadhyay, S.; Campbell, B.J.
Partial purification and characterization of the antidiuretic hormone-inactivating enzyme from renal plasma membranes
Biochim. Biophys. Acta
377
146-157
1975
Sus scrofa
brenda
Fruhaufova, L.; Suska-Brzezinska, E.; Barth,T.; Rychlik, I.
Rat liver enzyme inactivating oxytocin and its deamino-carba analogues
Collect. Czech. Chem. Commun.
38
2793-2798
1973
Rattus norvegicus
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brenda
Simmons, W.H.; Walter, R.
Carboxamidopeptidase: purification and characterization of a neurohypophyseal hormone inactivating peptidase from toad skin
Biochemistry
19
39-48
1980
toad
brenda
Simmons, W.H.
Peptidyl-glycinamidase
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds) Academic Press
2
1932-1933
2004
Rhinella marina
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brenda
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