Information on EC 3.4.14.2 - dipeptidyl-peptidase II and Organism(s) Homo sapiens

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria


The taxonomic range for the selected organisms is: Homo sapiens

EC NUMBER
COMMENTARY hide
3.4.14.2
-
RECOMMENDED NAME
GeneOntology No.
dipeptidyl-peptidase II
-
CAS REGISTRY NUMBER
COMMENTARY hide
76199-23-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-Ala-4-nitroanilide + H2O
Ala-Ala + 4-nitroanilide
show the reaction diagram
-
-
-
-
?
Ala-Ala-Ala + H2O
Ala-Ala + Ala
show the reaction diagram
-
54% activity compared to Met-Ala-Ser
-
-
?
Ala-Ala-Ala-Ala + H2O
Ala-Ala + Ala-Ala
show the reaction diagram
-
39% activity compared to Met-Ala-Ser
-
-
?
Ala-Pro-2-(4-methoxy)-naphthylamine + H2O
?
show the reaction diagram
-
-
-
-
?
Ala-Pro-4-nitroanilide + H2O
Ala-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Ala-Pro-amino-4-trifluoromethylcoumarin + H2O
Ala-Pro + amino-4-trifluoromethylcoumarin
show the reaction diagram
-
-
?
Ala-Pro-p-nitroanilide + H2O
Ala-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Arg-Pro-4-nitroanilide + H2O
Arg-Pro + 4-nitroanilide
show the reaction diagram
-
-
-
-
?
Arg-Pro-Lys-Pro + H2O
Arg-Pro + Lys-Pro
show the reaction diagram
-
fragments of substance P
-
-
?
Arg-Pro-p-nitroanilide + H2O
Arg-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Asn-Pro-p-nitroanilide + H2O
Asn-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Asp-Pro-4-nitroanilide + H2O
Asp-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
casomorphin + H2O
?
show the reaction diagram
-
-
-
-
?
Glu-Pro-4-nitroanilide + H2O
Glu-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Gly-Ala-7-amido-4-methylcoumarin + H2O
Gly-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Gly-Pro-4-nitroanilide + H2O
Gly-Pro + 4-nitroaniline
show the reaction diagram
Gly-Pro-7-amido-4-methylcoumarin + H2O
Gly-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Gly-Pro-Ala + H2O
Gly-Pro + Ala
show the reaction diagram
-
96% activity compared to Met-Ala-Ser
-
-
?
Gly-Pro-Gly-Gly + H2O
Gly-Pro + Gly-Gly
show the reaction diagram
-
16% activity compared to Met-Ala-Ser
-
-
?
Gly-Pro-p-nitroanilide + H2O
Gly-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
His-Ala-p-nitroanilide + H2O
His-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
His-Pro-p-nitroanilide + H2O
His-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Lys-Ala-4-methylcoumaryl-7-amide + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
Lys-Ala-7-amido-4-methylcoumarin + H2O
Lys-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Lys-Ala-p-nitroanilide + H2O
Lys-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Lys-Pro-2-(4-methoxy)-naphthylamine + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Pro-4-nitroanilide + H2O
Lys-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Lys-Pro-7-amido-4-methylcoumarin + H2O
Lys-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Lys-Pro-p-nitroanilide + H2O
Lys-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Met-Ala-Ser + H2O
Met-Ala + Ser
show the reaction diagram
-
100% activity
-
-
?
Nle-Nle-p-nitroanilide + H2O
Nle-Nle + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Nle-Nle-rhodamine + H2O
Nle-Nle + rhodamine
show the reaction diagram
-
-
?
Nle-Pro-7-amido-4-methylcoumarin + H2O
Nle-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Nle-Pro-rhodamine + H2O
Nle-Pro + rhodamine
show the reaction diagram
-
-
?
Phe-Pro-4-methylcoumarin 7-amide + H2O
Phe-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Phe-Pro-p-nitroanilide + H2O
Phe-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Pro-Ala-p-nitroanilide + H2O
Pro-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Ser-Pro-p-nitroanilide + H2O
Ser-Pro + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Substance P + H2O
?
show the reaction diagram
-
-
-
-
?
Tyr-Ala-p-nitroanilide + H2O
Tyr-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Arg-Pro-Lys-Pro + H2O
Arg-Pro + Lys-Pro
show the reaction diagram
-
fragments of substance P
-
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
casomorphin + H2O
?
show the reaction diagram
-
-
-
-
?
Substance P + H2O
?
show the reaction diagram
-
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,S)-(2E)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0361 mM, low inhibitory potential against EC 3.4.14.5
(1R,S)-(2E)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0404 mM, low inhibitory potential against EC 3.4.14.5
(1R,S)-(2Z)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0329 mM, low inhibitory potential against EC 3.4.14.5
(1R,S)-(2Z)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
-
IC50: 0.0873 mM, low inhibitory potential against EC 3.4.14.5
(3S)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
-
-
(3S)-4-[(3aR,6aR)-hexahydrocyclopenta[b]pyrrol-1(2H)-yl]-4-oxobutane-1,3-diamine
-
AX8819, potent and selective inhibitor of DPP II
(3S)-N1-(4-chlorobenzyl)-4-(2-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 2.3 mM; IC50: 2.3 nM
(3S)-N1-(4-chlorobenzyl)-4-(3-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
-
0.62 nM; IC50: 0.62 nM
(3S)-N1-(4-chlorobenzyl)-4-(3-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 1.13 nM
(3S)-N1-(4-chlorobenzyl)-4-(4-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 0.39 nM
(3S)-N1-(4-chlorobenzyl)-4-(4-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
-
IC50: 0.55 nM
(3S)-N1-(4-chlorobenzyl)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
1-((2S)-2-amino-4-((4-chlorobenzyl)amino)butanoyl)piperidin-3-ol
-
IC50: 54.7 nM
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidin-4-ol
-
IC50: 113 nM
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidine-2-carbonitrile
-
IC50: 6.5 nM
1-([(2-phenylethyl)amino]acetyl)-2-pyrrolidinecarbonitrile
-
0.0163 mM
1-([(4-fluorobenzyl)amino]acetyl)-2-pyrrolidinecarbonitrile
-
IC50: 0.0255 mM
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
-
IC50: 0.00104 mM, low inhibitory potential against EC 3.4.14.5
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)piperidin-4-amine
-
-
1-benzyl-N-(2-cyclopentylidene-2-fluoroethyl)-4-piperidinamine
-
IC50: 0.0013 mM, low inhibitory potential against EC 3.4.14.5
1-benzyl-N-(2-oxo-2-piperidin-1-ylethyl)piperidin-4-amine
-
-
1-benzyl-N-[2-oxo-2-(1-piperidinyl)ethyl]-4-piperidinamine
-
IC50: 0.0031 mM, low inhibitory potential against EC 3.4.14.5
1-[(1-adamantylamino)acetyl]-2-pyrrolidinecarbonitrile
-
IC50: 0.1335 mM
1-[(2S)-2-amino-4-(benzylamino)butanoyl]piperidine-2-carbonitrile
-
IC50: 4.0 nM
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-3-ol
-
IC50: 54.7 nM
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-4-ol
-
IC50: 113 nM
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidine-2-carbonitrile
-
IC50: 6.5 nM
3-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)aminomethyl)-benzonitrile
-
IC50: 2.7 nM
4-oxo-4-(1-piperidinyl)-N1 -(4-piperidinyl)-1,3-(S)-butanedi-amine
-
IC50: 85 nM
4-oxo-4-(1-piperidinyl)-N1-(2-thienylmethyl)-1,3-(S)-butanediamine
-
IC50: 0.8 nM
4-oxo-4-(1-piperidinyl)-N1-(4-pyridinylmethyl)-1,3-(S)-butanediamine
-
IC50: 0.75 nM
4-oxo-4-(1-piperidinyl)-N3-(4-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.017 mM
4-oxo-N1-(2-phenylethyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.9 nM
4-oxo-N1-(3-phenylpropyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.91 nM
4-oxo-N1-phenyl-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.0015 mM
6-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)amino)nicotinonitrile
-
IC50: 0.0046 mM
Ala-PSI[CS-N]-pyrrolidine
-
competitive
Ala-PSI[CS-N]-thiazolidine
-
competitive
Ala-pyrrolidine
-
competitive
Ala-thiazolidine
-
competitive
benzyl 4-[(2-cyclohexylidene-2-fluoroethyl)amino]-1-piperidinecarboxylate
-
IC50: 0.0128 mM, low inhibitory potential against EC 3.4.14.5
bestatin
-
-
Cd2+
-
-
Co2+
-
-
Cu2+
-
-
diisopropyl fluorophosphate
-
-
Diprotin A
-
not selective for the enzyme
EDTA
-
-
Hg2+
-
-
Ile cyanothiazolidide
-
not selective for the enzyme
Ile-PSI[CS-N]-pyrrolidine
-
competitive
Ile-PSI[CS-N]-thiazolidine
-
competitive
Ile-pyrrolidine
-
competitive
Ile-thiazolidine
-
competitive
Lys piperidide
-
not selective for the enzyme
Lys thiazolidide
-
not selective for the enzyme
lysyl-piperidide
-
-
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclohexylidene-2-fluoroethanamine
-
IC50: 0.0129 mM, low inhibitory potential against EC 3.4.14.5
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclopentylideneethanamine
-
0.0075 mM; IC50: 0.0075 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-1-adamantanamine
-
IC50: 0.0913 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
-
IC50: 0.0042 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-N-(2-phenylethyl)-amine
-
IC50: 0.011 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-fluorobenzyl)-amine
-
IC50: 0.0219 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-methoxybenzyl)amine
-
IC50: 0.0134 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)aniline
-
IC50: 0.1 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclohexylidene-2-fluoroethyl)cyclohexanamine
-
IC50: 0.5 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-phenylethyl)amine
-
IC50: 0.0272 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-pyridinylethyl)amine
-
IC50: 0.0096 mM, low inhibitory potential against EC 3.4.14.5; IC50: 0.0157 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-fluorobenzyl)amine
-
IC50: 0.0108 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-methoxy-benzyl)amine
-
IC50: 0.0155 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylidene-2-fluoroethyl)cyclohexanamine
-
IC50: 0.09 mM, low inhibitory potential against EC 3.4.14.5
N-(2-cyclopentylideneethyl)cyclohexanamine
-
IC50: 0.062 mM; IC50: 0.062 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
-
-
N-benzyl-2-cyclohexylidene-2-fluoroethanamine
-
IC50: 0.034 mM, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-cyclopentylidene-2-fluoroethanamine
-
IC50: 0.0378 mM, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-cyclopentylideneethanamine
-
IC50: 0.026 mM; IC50: 0.026 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-oxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.397 mM, low inhibitory potential against EC 3.4.14.5
N-benzyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.5 mM
N-benzyl-2-thioxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.0484 mM, low inhibitory potential against EC 3.4.14.5
N-cyclohexyl-2-oxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.177 mM, low inhibitory potential against EC 3.4.14.5
N-cyclohexyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.276 mM
N-cyclohexyl-2-thioxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.069 mM, low inhibitory potential against EC 3.4.14.5
N-cyclohexyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.092 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-oxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.052 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.063 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-thioxo-2-(1-piperidinyl)ethaneamine
-
IC50: 0.042 mM, low inhibitory potential against EC 3.4.14.5
N-piperonyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
-
IC50: 0.067 mM, low inhibitory potential against EC 3.4.14.5
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]acetamide
-
IC50: 0.0015 mM
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]guanidine bis-(trifluoroacetate)
-
IC50: 0.0018 mM
N-[3-amino-1-(S)-(1-piperidinylcarbonyl)propyl]guanidine bis-(trifluoroacetate)
-
IC50: 0.0074 mM
N-[3-amino-4-oxo-4-(1-piperidinyl)butyl]benzamide
-
IC50: 0.0074 mM
N1 -(2-cyclopentylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
-
IC50: 0.0537 mM, low inhibitory potential against EC 3.4.14.5
N1 -(4-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.66 nM
N1,N1-di(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 4.2 nM
N1,N1-dibenzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.6 nM
N1-(1-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.31 nM
N1-(2,4-dimethoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 2.7 nM
N1-(2-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.23 nM
N1-(2-cyclohexylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
-
IC50: 0.0546 mM, low inhibitory potential against EC 3.4.14.5
N1-(2-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.85 nM
N1-(2-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.1 nM
N1-(3-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.34 nM
N1-(3-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.77 nM
N1-(3-nitro-2-pyridinyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.0235 mM
N1-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.48 nM
N1-(4-chlorobenzyl)-N1-methyl-4-oxo-4-(1-piperidinyl)-1,3-butanediamine
-
IC50: 0.22 nm
N1-(cyclohexylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 1.1 nM
N1-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 2.03 nM
N1-cyclohexyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 18.6 nM
N1-[2-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.39 nM
N1-[4-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
-
IC50: 0.41 nM
N2-cyclopentyl-N-[(diphenoxyphosphoryl)(phenyl)methyl]glycinamide
-
-
N3-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine dihydrobromide
-
IC50: 0.0125 mM
Phe-PSI[CS-N]-pyrrolidine
-
competitive
Phe-PSI[CS-N]-thiazolidine
-
competitive
Phe-pyrrolidine
-
competitive
Phe-thiazolidine
-
competitive
phenylmethylsulfonyl fluoride
-
-
threo-Ile thiazolidide
-
not selective for the enzyme
Val pyrrolidide
-
not selective for the enzyme
Val thiazolidide
-
not selective for the enzyme
Val-PSI[CS-N]-pyrrolidine
-
competitive
Val-PSI[CS-N]-thiazolidine
-
competitive
Val-pyrrolidine
-
competitive
Val-thiazolidine
-
competitive
[(1R)-1-[[(2S)-2,4-diaminobutanoyl]amino]pentyl]boronic acid
-
-
additional information
-
isomerization of the thiazolidine derivatives
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.22
Ala-Ala-4-nitroanilide
-
-
0.0457
Ala-Pro-4-nitroanilide
-
-
0.032
Ala-Pro-amino-4-trifluoromethylcoumarin
-
pH 7.5, 37°C
0.009 - 0.041
Ala-Pro-p-nitroanilide
0.086
Arg-Pro-4-nitroanilide
-
-
0.04
Arg-Pro-p-nitroanilide
-
37°C, pH 5.5
0.101
Asn-Pro-p-nitroanilide
-
37°C, pH 5.5
1.16
Asp-Pro-4-nitroanilide
-
-
0.311
Glu-Pro-4-nitroanilide
-
-
0.25 - 0.282
Gly-Pro-4-nitroanilide
0.149
Gly-Pro-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.07 - 0.227
Gly-Pro-p-nitroanilide
0.0223
His-Ala-p-nitroanilide
-
37°C, pH 5.5
0.083
His-Pro-p-nitroanilide
-
37°C, pH 5.5
0.93
Lys-Ala-4-methylcoumarin 7-amide
-
-
0.215
Lys-Ala-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.04 - 0.57
Lys-Ala-p-nitroanilide
0.0457
Lys-Pro-4-nitroanilide
-
-
0.011
Lys-Pro-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.019
Lys-Pro-p-nitroanilide
0.02
Nle-Nle-p-nitroanilide
-
37°C, pH 5.5
0.006
Nle-Nle-rhodamine
-
pH 7.5, 37°C
0.003
Nle-Pro-7-amido-4-methylcoumarin
-
pH 7.5, 37°C
0.003
Nle-Pro-rhodamine
-
pH 7.5, 37°C
0.058
Phe-Pro-p-nitroanilide
-
37°C, pH 5.5
1.376
Pro-Ala-p-nitroanilide
-
37°C, pH 5.5
0.08
Ser-Pro-p-nitroanilide
-
37°C, pH 5.5
0.22
Tyr-Ala-p-nitroanilide
-
37°C, pH 5.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
89 - 105.4
Ala-Pro-p-nitroanilide
65.8
Arg-Pro-p-nitroanilide
-
37°C, pH 5.5
184.6
Asn-Pro-p-nitroanilide
-
37°C, pH 5.5
67.3
Gly-Pro-p-nitroanilide
12.8
His-Ala-p-nitroanilide
-
37°C, pH 5.5
28.4
His-Pro-p-nitroanilide
-
37°C, pH 5.5
229.3
Lys-Ala-p-nitroanilide
-
37°C, pH 5.5
3 - 77.7
Lys-Pro-p-nitroanilide
18.4
Nle-Nle-p-nitroanilide
-
37°C, pH 5.5
93
Phe-Pro-p-nitroanilide
-
37°C, pH 5.5
15.3
Pro-Ala-p-nitroanilide
-
37°C, pH 5.5
66 - 116.9
Ser-Pro-p-nitroanilide
11
Tyr-Ala-p-nitroanilide
-
37°C, pH 5.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0033
Ala-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30°C
0.00065
Ala-PSI[CS-N]-thiazolidine
-
pH 5.5, 30°C
0.0218
Ala-pyrrolidine
-
pH 5.5, 30°C
0.0044
Ala-thiazolidine
-
pH 5.5, 30°C
0.0408
Diprotin A
-
pH 7.5, 37°C
0.00056
Ile cyanothiazolidide
-
pH 7.5, 37°C
0.0048
Ile-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30°C
0.0011
Ile-PSI[CS-N]-thiazolidine
-
pH 5.5, 30°C
0.0302
Ile-pyrrolidine
-
pH 5.5, 30°C
0.0071
Ile-thiazolidine
-
pH 5.5, 30°C
0.00065
Lys piperidide
-
pH 7.5, 37°C
0.00017
Lys thiazolidide
-
pH 7.5, 37°C
0.000052 - 0.00264
lysyl-piperidide
0.000082 - 0.00145
N-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butane-diamine dihydrochloride
0.0073
Phe-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30°C
0.0006
Phe-PSI[CS-N]-thiazolidine
-
pH 5.5, 30°C
0.0139
Phe-pyrrolidine
-
pH 5.5, 30°C
0.0032
Phe-thiazolidine
-
pH 5.5, 30°C
0.0097
threo-Ile thiazolidide
-
pH 7.5, 37°C
0.1
Val pyrrolidide
-
above, pH 7.5, 37°C
0.0153
Val thiazolidide
-
pH 7.5, 37°C
0.0082
Val-PSI[CS-N]-pyrrolidine
-
pH 5.5, 30°C
0.0009
Val-PSI[CS-N]-thiazolidine
-
pH 5.5, 30°C
0.0267
Val-pyrrolidine
-
pH 5.5, 30°C
0.0077
Val-thiazolidine
-
pH 5.5, 30°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0361
(1R,S)-(2E)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens;
-
IC50: 0.0361 mM, low inhibitory potential against EC 3.4.14.5
0.0404
(1R,S)-(2E)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens;
-
IC50: 0.0404 mM, low inhibitory potential against EC 3.4.14.5
0.0329
(1R,S)-(2Z)-2-(1-fluoro-2-[(2-phenylethyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens;
-
IC50: 0.0329 mM, low inhibitory potential against EC 3.4.14.5
0.0873
(1R,S)-(2Z)-2-(1-fluoro-2-[(4-fluorobenzyl)amino]ethylidene)cyclopentanecarbonitrile
Homo sapiens;
-
IC50: 0.0873 mM, low inhibitory potential against EC 3.4.14.5
0.00013
(3S)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
Homo sapiens;
-
-
0.00000088
(3S)-4-[(3aR,6aR)-hexahydrocyclopenta[b]pyrrol-1(2H)-yl]-4-oxobutane-1,3-diamine
Homo sapiens;
-
-
0.0000023 - 2.3
(3S)-N1-(4-chlorobenzyl)-4-(2-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
0.62
(3S)-N1-(4-chlorobenzyl)-4-(3-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens;
-
IC50: 0.62 nM
0.00000113
(3S)-N1-(4-chlorobenzyl)-4-(3-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens;
-
IC50: 1.13 nM
0.00000039
(3S)-N1-(4-chlorobenzyl)-4-(4-fluoropiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens;
-
IC50: 0.39 nM
0.00000055
(3S)-N1-(4-chlorobenzyl)-4-(4-methylpiperidin-1-yl)-4-oxobutane-1,3-diamine
Homo sapiens;
-
IC50: 0.55 nM
0.00000048 - 0.0000011
(3S)-N1-(4-chlorobenzyl)-4-oxo-4-piperidin-1-ylbutane-1,3-diamine
0.0000547
1-((2S)-2-amino-4-((4-chlorobenzyl)amino)butanoyl)piperidin-3-ol
Homo sapiens;
-
IC50: 54.7 nM
0.000113
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidin-4-ol
Homo sapiens;
-
IC50: 113 nM
0.0000065
1-((2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl)piperidine-2-carbonitrile
Homo sapiens;
-
IC50: 6.5 nM
0.0255
1-([(4-fluorobenzyl)amino]acetyl)-2-pyrrolidinecarbonitrile
Homo sapiens;
-
IC50: 0.0255 mM
0.00104
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
Homo sapiens;
-
IC50: 0.00104 mM, low inhibitory potential against EC 3.4.14.5
0.00104
1-benzyl-N-(2-cyclohexylidene-2-fluoroethyl)piperidin-4-amine
Homo sapiens;
-
-
0.0013
1-benzyl-N-(2-cyclopentylidene-2-fluoroethyl)-4-piperidinamine
Homo sapiens;
-
IC50: 0.0013 mM, low inhibitory potential against EC 3.4.14.5
0.0031
1-benzyl-N-(2-oxo-2-piperidin-1-ylethyl)piperidin-4-amine
Homo sapiens;
-
-
0.0031
1-benzyl-N-[2-oxo-2-(1-piperidinyl)ethyl]-4-piperidinamine
Homo sapiens;
-
IC50: 0.0031 mM, low inhibitory potential against EC 3.4.14.5
0.1335
1-[(1-adamantylamino)acetyl]-2-pyrrolidinecarbonitrile
Homo sapiens;
-
IC50: 0.1335 mM
0.000004
1-[(2S)-2-amino-4-(benzylamino)butanoyl]piperidine-2-carbonitrile
Homo sapiens;
-
IC50: 4.0 nM
0.0000547
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-3-ol
Homo sapiens;
-
IC50: 54.7 nM
0.000113
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidin-4-ol
Homo sapiens;
-
IC50: 113 nM
0.0000065
1-[(2S)-2-amino-4-[(4-chlorobenzyl)amino]butanoyl]piperidine-2-carbonitrile
Homo sapiens;
-
IC50: 6.5 nM
0.0000027
3-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)aminomethyl)-benzonitrile
Homo sapiens;
-
IC50: 2.7 nM
0.000085
4-oxo-4-(1-piperidinyl)-N1 -(4-piperidinyl)-1,3-(S)-butanedi-amine
Homo sapiens;
-
IC50: 85 nM
0.0000008
4-oxo-4-(1-piperidinyl)-N1-(2-thienylmethyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.8 nM
0.00000075
4-oxo-4-(1-piperidinyl)-N1-(4-pyridinylmethyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.75 nM
0.017
4-oxo-4-(1-piperidinyl)-N3-(4-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.017 mM
0.0000009
4-oxo-N1-(2-phenylethyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.9 nM
0.00000191
4-oxo-N1-(3-phenylpropyl)-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 1.91 nM
0.0015
4-oxo-N1-phenyl-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.0015 mM
0.0046
6-((3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl)amino)nicotinonitrile
Homo sapiens;
-
IC50: 0.0046 mM
0.0128
benzyl 4-[(2-cyclohexylidene-2-fluoroethyl)amino]-1-piperidinecarboxylate
Homo sapiens;
-
IC50: 0.0128 mM, low inhibitory potential against EC 3.4.14.5
0.0129
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclohexylidene-2-fluoroethanamine
Homo sapiens;
-
IC50: 0.0129 mM, low inhibitory potential against EC 3.4.14.5
0.0075
N-(1,3-benzodioxol-5-ylmethyl)-2-cyclopentylideneethanamine
Homo sapiens;
-
IC50: 0.0075 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
0.0913
N-(2-cyclohexylidene-2-fluoroethyl)-1-adamantanamine
Homo sapiens;
-
IC50: 0.0913 mM, low inhibitory potential against EC 3.4.14.5
0.0042
N-(2-cyclohexylidene-2-fluoroethyl)-4-piperidinamine
Homo sapiens;
-
IC50: 0.0042 mM, low inhibitory potential against EC 3.4.14.5
0.011
N-(2-cyclohexylidene-2-fluoroethyl)-N-(2-phenylethyl)-amine
Homo sapiens;
-
IC50: 0.011 mM, low inhibitory potential against EC 3.4.14.5
0.0219
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-fluorobenzyl)-amine
Homo sapiens;
-
IC50: 0.0219 mM, low inhibitory potential against EC 3.4.14.5
0.0134
N-(2-cyclohexylidene-2-fluoroethyl)-N-(4-methoxybenzyl)amine
Homo sapiens;
-
IC50: 0.0134 mM, low inhibitory potential against EC 3.4.14.5
0.1
N-(2-cyclohexylidene-2-fluoroethyl)aniline
Homo sapiens;
-
IC50: 0.1 mM, low inhibitory potential against EC 3.4.14.5
0.5
N-(2-cyclohexylidene-2-fluoroethyl)cyclohexanamine
Homo sapiens;
-
IC50: 0.5 mM, low inhibitory potential against EC 3.4.14.5
0.0272
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-phenylethyl)amine
Homo sapiens;
-
IC50: 0.0272 mM, low inhibitory potential against EC 3.4.14.5
0.0096 - 0.0157
N-(2-cyclopentylidene-2-fluoroethyl)-N-(2-pyridinylethyl)amine
0.0108
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-fluorobenzyl)amine
Homo sapiens;
-
IC50: 0.0108 mM, low inhibitory potential against EC 3.4.14.5
0.0155
N-(2-cyclopentylidene-2-fluoroethyl)-N-(4-methoxy-benzyl)amine
Homo sapiens;
-
IC50: 0.0155 mM, low inhibitory potential against EC 3.4.14.5
0.09
N-(2-cyclopentylidene-2-fluoroethyl)cyclohexanamine
Homo sapiens;
-
IC50: 0.09 mM, low inhibitory potential against EC 3.4.14.5
0.062
N-(2-cyclopentylideneethyl)cyclohexanamine
Homo sapiens;
-
IC50: 0.062 mM; IC50: 0.062 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
0.034
N-benzyl-2-cyclohexylidene-2-fluoroethanamine
Homo sapiens;
-
IC50: 0.034 mM, low inhibitory potential against EC 3.4.14.5
0.0378
N-benzyl-2-cyclopentylidene-2-fluoroethanamine
Homo sapiens;
-
IC50: 0.0378 mM, low inhibitory potential against EC 3.4.14.5
0.026
N-benzyl-2-cyclopentylideneethanamine
Homo sapiens;
-
IC50: 0.026 mM; IC50: 0.026 mM, low inhibitory potential against EC 3.4.14.5, low inhibitory potential against EC 3.4.14.5
0.397
N-benzyl-2-oxo-2-(1-piperidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.397 mM, low inhibitory potential against EC 3.4.14.5
0.5
N-benzyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.5 mM
0.0484
N-benzyl-2-thioxo-2-(1-piperidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.0484 mM, low inhibitory potential against EC 3.4.14.5
0.177
N-cyclohexyl-2-oxo-2-(1-piperidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.177 mM, low inhibitory potential against EC 3.4.14.5
0.276
N-cyclohexyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.276 mM
0.069
N-cyclohexyl-2-thioxo-2-(1-piperidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.069 mM, low inhibitory potential against EC 3.4.14.5
0.092
N-cyclohexyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.092 mM, low inhibitory potential against EC 3.4.14.5
0.052
N-piperonyl-2-oxo-2-(1-piperidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.052 mM, low inhibitory potential against EC 3.4.14.5
0.063
N-piperonyl-2-oxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.063 mM, low inhibitory potential against EC 3.4.14.5
0.042
N-piperonyl-2-thioxo-2-(1-piperidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.042 mM, low inhibitory potential against EC 3.4.14.5
0.067
N-piperonyl-2-thioxo-2-(1-pyrrolidinyl)ethaneamine
Homo sapiens;
-
IC50: 0.067 mM, low inhibitory potential against EC 3.4.14.5
0.0015
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]acetamide
Homo sapiens;
-
IC50: 0.0015 mM
0.0018
N-[3-(S)-amino-4-oxo-4-(1-piperidinyl)butyl]guanidine bis-(trifluoroacetate)
Homo sapiens;
-
IC50: 0.0018 mM
0.0074
N-[3-amino-1-(S)-(1-piperidinylcarbonyl)propyl]guanidine bis-(trifluoroacetate)
Homo sapiens;
-
IC50: 0.0074 mM
0.0074
N-[3-amino-4-oxo-4-(1-piperidinyl)butyl]benzamide
Homo sapiens;
-
IC50: 0.0074 mM
0.0537
N1 -(2-cyclopentylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
Homo sapiens;
-
IC50: 0.0537 mM, low inhibitory potential against EC 3.4.14.5
0.00000066
N1 -(4-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.66 nM
0.0000042
N1,N1-di(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 4.2 nM
0.0000006
N1,N1-dibenzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.6 nM
0.00000131
N1-(1-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 1.31 nM
0.0000027
N1-(2,4-dimethoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 2.7 nM
0.00000023
N1-(2-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.23 nM
0.0546
N1-(2-cyclohexylidene-2-fluoroethyl)-N3,N3-dimethyl-1,3-propanediamine
Homo sapiens;
-
IC50: 0.0546 mM, low inhibitory potential against EC 3.4.14.5
0.00000185
N1-(2-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 1.85 nM
0.0000011
N1-(2-naphthylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 1.1 nM
0.00000134
N1-(3-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 1.34 nM
0.00000177
N1-(3-methoxybenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 1.77 nM
0.0235
N1-(3-nitro-2-pyridinyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.0235 mM
0.00000048
N1-(4-chlorobenzyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.48 nM
0.00000022
N1-(4-chlorobenzyl)-N1-methyl-4-oxo-4-(1-piperidinyl)-1,3-butanediamine
Homo sapiens;
-
IC50: 0.22 nm
0.0000011
N1-(cyclohexylmethyl)-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 1.1 nM
0.00000203
N1-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 2.03 nM
0.0000186
N1-cyclohexyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 18.6 nM
0.00000039
N1-[2-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.39 nM
0.00000041
N1-[4-(benzyloxy)benzyl]-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine
Homo sapiens;
-
IC50: 0.41 nM
0.0038
N2-cyclopentyl-N-[(diphenoxyphosphoryl)(phenyl)methyl]glycinamide
Homo sapiens;
-
-
0.0125
N3-benzyl-4-oxo-4-(1-piperidinyl)-1,3-(S)-butanediamine dihydrobromide
Homo sapiens;
-
IC50: 0.0125 mM
0.00000048
[(1R)-1-[[(2S)-2,4-diaminobutanoyl]amino]pentyl]boronic acid
Homo sapiens;
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
substrate: Lys-Ala-p-nitroanilide
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
substrate: Lys-Ala-p-nitroanilide or Ala-Pro-p-nitroanilide
5 - 5.5
-
enzyme from seminal fluid
5.5 - 6
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 8.5
-
-
4 - 6
-
pH 4.0: about 50% of maximal activity, pH 6.0: about 40% of maximal activity, substrate: Lys-Ala-p-nitroanilide or Ala-Pro-p-nitroanilide
4 - 7
-
50% of maximal activity at pH 4 and 7
5 - 6
-
-
5.5 - 7
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
37
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8
-
isoelectric focusing, enzyme from spleen and ovary
6.1
-
isoelectric focusing, enzyme from seminal plasma
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest activity in the hypothalamus
Manually annotated by BRENDA team
-
of patients with Sjorgren's syndrome and healthy subjects
Manually annotated by BRENDA team
-
increased activity in serum of cancer patients
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Homo sapiens;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
-
gel filtration, enzyme from kidney
116000
-
SDS-PAGE
120000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
2 h, 30% loss of activity without a stabilizer. 10% loss of activity after 4 h in presence of 1 mg/ml bovine serum albumin or 0.1% Tween 20
50
-
stable up to 50°C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
in presence of stabilizing agents, the enzyme tolerates at least five subsequent freeze/thaw cycles
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, -20°C, or -80°C, purified enzyme is stable for at least 4 months in presence of 1 mg/ml bovine serum albumin
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from placenta
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His·Bind Resin column chromatography
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purification scheme includes an inhibitor-based affinity chromatographic step
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in 293-EBNA cells
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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the enzyme is a potential urinary marker for the biochemical detection of kidney damage