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The expected taxonomic range for this enzyme is: Brevibacterium fuscum
Synonyms
isomaltotrio-dextranase,
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exo-isomaltotriohydrolase
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exoisomaltotriohydrolase
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isomaltotrio-dextranase
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isomaltotriohydrolase, exo-
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(Glcalpha(1-6))n + H2O = (Glcalpha(1-6))n-3 + isomaltotriose
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hydrolysis of O-glycosyl bond
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6-alpha-D-glucan isomaltotriohydrolase
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dextran + H2O
isomaltotriose
isomaltodextrin + H2O
isomaltotriose
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reduced
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Sephadex G-100 + H2O
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Sephadex G-200 + H2O
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dextran + H2O
isomaltotriose
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dextran + H2O
isomaltotriose
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dextran + H2O
isomaltotriose
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AgNO3
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2 mM, 97% inhibition
CuSO4
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2 mM, 76% inhibition
HgCl2
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2 mM, complete inhibition
Iodine
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2 mM, complete inhibition
N-bromosuccinimide
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2 mM, complete inhibition
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additional information
additional information
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Km: 0.72 mg/ml for the recombinant enzyme and 0.26 mg/ml for the native enzyme
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21.5
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recombinant enzyme
additional information
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6.5 - 8
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85% of maximum activity at pH 6.5 and pH 8.0
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strain 0407
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brenda
strain 0407
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brenda
var. dextranlyticum
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brenda
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Q9WXI3_9MICO
641
0
71989
TrEMBL
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Q934J4_9MICO
25
0
2527
TrEMBL
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68000
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x * 68000, SDS-PAGE
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x * 68000, SDS-PAGE
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x * 68000, SDS-PAGE
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5.6 - 11
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37°C, 12 h, stable
208775
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50
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pH 7.5, 30 min, stable up to
60
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pH 7.5, 30 min, 30% loss of activity
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expression in Escherichia coli
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Sugiura, M.; Ito, A.; Yamaguchi, T.
Studies on dextranase. II. New exo-dextranase from Brevibacterium fuscum var. Dextranlyticum
Biochim. Biophys. Acta
350
61-70
1974
Brevibacterium fuscum
brenda
Mizuno, T.; Mori, H.; Ito, H.; Matsui, H.; Kimura, A.; Chiba, S.
Molecular cloning of isomaltotrio-dextranase gene from Brevibacterium fuscum var. dextranlyticum strain 0407 and its expression in Escherichia coli
Biosci. Biotechnol. Biochem.
63
1582-1588
1999
Brevibacterium fuscum, Brevibacterium fuscum 407
brenda
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3.2.1.95 dextran 1,6-alpha-isomaltotriosidase
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