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EC Tree
IUBMB Comments Products are nigerose and 4-alpha-D-nigerosylglucose. No hydrolysis of alpha-D-glucans containing only 1,3- or 1,4-bonds.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
dextranase, myco-,
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dextranase, myco-
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Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
enzyme hydrolyzes nigeran tetrasaccharide by an endo-type of action
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Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
enzyme hydrolyzes nigeran tetrasaccharide by an endo-type of action
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Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
endo-multichain mechanism
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Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
endo-multichain mechanism
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Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
enzyme hydrolyzes nigeran tetrasaccharide by an endo-type of action
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Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
endo-multichain mechanism
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Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
enzyme hydrolyzes nigeran tetrasaccharide by an endo-type of action
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Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
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hydrolysis of O-glycosyl bond
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(1->3)-(1->4)-alpha-D-glucan 4-glucanohydrolase
Products are nigerose and 4-alpha-D-nigerosylglucose. No hydrolysis of alpha-D-glucans containing only 1,3- or 1,4-bonds.
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nigeran + H2O
nigerose + 4-alpha-D-nigerosylglucose
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
nigeran + H2O
nigerose + 4-alpha-D-nigerosylglucose
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?
nigeran + H2O
nigerose + 4-alpha-D-nigerosylglucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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both, fast and slow enzyme
no production of glucose
?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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both, fast and slow enzyme
no production of glucose
?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
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?
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nigeran + H2O
nigerose + 4-alpha-D-nigerosylglucose
nigeran + H2O
nigerose + 4-alpha-D-nigerosylglucose
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?
nigeran + H2O
nigerose + 4-alpha-D-nigerosylglucose
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?
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nigeran tetrasaccharide
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nigeran trisaccharide
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p-chloromercuribenzoic acid
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Hg+
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fast enzyme
Hg2+
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N-bromosuccinimide
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N-bromosuccinimide
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fast enzyme
N-Chlorosuccinimide
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N-Chlorosuccinimide
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fast enzyme
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additional information
additional information
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additional information
additional information
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additional information
additional information
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6
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2 - 9
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fast and slow enzyme pH 2 15%, pH 9 75% of maximal activity
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70
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fast and slow enzyme
50
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23 - 80
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23°C: fast enzyme 7%, slow enzyme 16% of maximal activity, 80°C: fast enzyme 50%, slow enzyme 30% of maximal activity
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NHB-1
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brenda
NHB-1
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brenda
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brenda
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brenda
QM 1931
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brenda
IFO 3355
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brenda
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brenda
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brenda
QM 1931
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SwissProt
brenda
J-13-3
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brenda
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SwissProt
brenda
J-13-3
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brenda
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brenda
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brenda
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A0A2Z5WLI6_BACCI
1042
1
107833
TrEMBL
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Q93R89_9ACTN
600
0
62245
TrEMBL
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A0A3G9DKD4_ARTSP
990
1
99971
TrEMBL
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40000
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sedimentation equilibrium fast and slow enzyme
56078
x * 56078, calculation from nucleotide sequence
68000
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1 * 68000, SDS-PAGE
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?
x * 56078, calculation from nucleotide sequence
?
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x * 56078, calculation from nucleotide sequence
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monomer
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1 * 120000, SDS-PAGE
monomer
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1 * 120000, SDS-PAGE
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monomer
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1 * 40000, guanidine hydrochloride treatment
monomer
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1 * 40000, guanidine hydrochloride treatment
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monomer
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1 * 68000, SDS-PAGE
monomer
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1 * 68000, SDS-PAGE
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additional information
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fast and slow enzyme have similar but not identical amino acid compositions
additional information
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fast and slow enzyme have similar but not identical amino acid compositions
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glycoprotein
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glycoprotein
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fast form: 16% carbohydrate, slow form: 15% carbohydrate, only mannose and glucose
glycoprotein
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chemical constitution of carbohydrate portion
glycoprotein
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fast form: 16% carbohydrate, slow form: 15% carbohydrate, only mannose and glucose
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4 - 5
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stable between
136743
5 - 10
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80% of maximal activity after incubation at 4°C for 48 h
136748
6 - 7
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stable between
136748
6 - 8
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stable between
136747
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50
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enzyme stable up to 50°C
60
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10 min, 67% of maximal activity
60
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10 min, 60% of maximal activity
70
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10 min, 17% of maximal activity
70
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10 min, 22% of maximal activity
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4°C, 2 months, no loss of activity, fast and slow enzyme
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fast and slow enzyme, different electrophoretic mobilities
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synthesis
the enzyme seems to be a useful tool for the preparation of expensive nigerose and nigerooligosaccharides
synthesis
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the enzyme seems to be a useful tool for the preparation of expensive nigerose and nigerooligosaccharides
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Tung, K.K.; Nordin, J.H.
Structure of the tetrasaccharide produced by the hydrolysis of nigeran by the enzyme mycodextranase
Biochim. Biophys. Acta
158
154-156
1968
Penicillium melinii
brenda
Tung, K.K.; Rosenthal, A.; Nordin, J.H.
Enzymes that hydrolyze fungal cell wall polysaccharides. II. Purification and properties of mycodextranase, an endo -D-(1-4) glucanase from Penicillium melinii
J. Biol. Chem.
246
6722-6736
1971
Penicillium melinii, Penicillium melinii QM 1931
brenda
Samuel, O.; Nordin, J.H.
A unique system for the study of the biosynthesis and secretion of a specific glycoprotein enzyme
Biochem. Biophys. Res. Commun.
45
1376-1383
1971
Penicillium melinii, Penicillium melinii QM 1931
brenda
Rosenthal, A.L.; Nordin, J.H.
Enzymes that hydrolyze fungal cell wall polysaccharides.The carbohydrate constitution of mycodextranase, an endo -D-(1-4) glucanase from Penicillium melinii
J. Biol. Chem.
256
5295-5303
1975
Penicillium melinii
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brenda
Marchessault, R.H.; Revol, J.F.; Bobbitt, F.; Nordin, J.H.
Enzymatic depolymerization of lamellar single crystals of nigeran
Biopolymers
19
1069-1080
1980
Penicillium melinii
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brenda
Okazaki, K.; Abe, T.; Saruwatari, K.; Kato, F.; Maruyama, K.; Tagawa, K.
Purification and properties of mycodextranase from Streptomyces sp. J-13-3
Biosci. Biotechnol. Biochem.
56
1401-1405
1992
Streptomyces sp., Chloridium sp., Cladosporium sp., Penicillium melinii, Streptomyces griseus, Trichoderma sp., Streptomyces sp. J-13-3
brenda
Okazaki, K.; Jin, S.Y.; Sawamura, Y.; Hayakawa, S.
Purification and properties of mycodextranase from Bacillus circulans NHB-1
J. Ferment. Bioeng.
79
370-372
1995
Bacillus circulans, Penicillium melinii, Bacillus circulans NHB-1
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brenda
Okazaki, K.; Amano, T.; Morimoto, T.; Iemoto, T.; Kawabata, T.; Hayakawa, S.; Akimitsu, K.
Cloning and nucleotide sequence of the mycodextranase gene from Streptomyces sp. J-13-3
Biosci. Biotechnol. Biochem.
65
1684-1687
2001
Streptomyces sp. (Q93R89), Streptomyces sp. J-13-3 (Q93R89)
brenda
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3.2.1.61 mycodextranase
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