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Information on EC 3.2.1.22 - alpha-galactosidase and Organism(s) Homo sapiens

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EC Tree
IUBMB Comments
Also hydrolyses alpha-D-fucosides.
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
1,6-alpha-D-galactoside galactohydrolase, a-galactosidase, Ag-I, Ag-II, Aga-F78, Aga-Y, AgaA, agaAJB13, AgaB, AgaI, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Agalsidase alfa
alpha-D-galactosidase
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-
-
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Alpha-D-galactoside galactohydrolase
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-
-
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alpha-Gal
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alpha-Gal A
alpha-galactosidase
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alpha-galactosidase A
alpha-galactoside galactohydrolase
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-
-
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Fabrazyme
commercial preparation
Genzyme
commercial preparation
melibiase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-35-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl alpha-D-galactopyranoside + H2O
4-methylumbelliferone + alpha-D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
alpha-D-galactose + 4-methylumbelliferone
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-galactoside + H2O
4-methylumbelliferol + alpha-D-galactose
show the reaction diagram
-
-
-
-
-
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
galactobisylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
globopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
globotriaosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
globotriaosylceramide + H2O
D-Galalpha(1,4)D-Glu-ceramide + D-galactose
show the reaction diagram
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i.e. D-Galalpha(1,4)D-Galalpha(1,4)D-Glu-ceramide
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-
?
globotriose + H2O
?
show the reaction diagram
-
-
-
-
?
globtriglycosylceramide + H2O
?
show the reaction diagram
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-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl alpha-D-fucopyranoside + H2O
o-nitrophenol + D-fucose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-alpha-N-acetyl-galactosaminide + H2O
?
show the reaction diagram
-
-
-
-
?
trihexosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
globotriaosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
globotriaosylceramide + H2O
D-Galalpha(1,4)D-Glu-ceramide + D-galactose
show the reaction diagram
-
i.e. D-Galalpha(1,4)D-Galalpha(1,4)D-Glu-ceramide
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-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
trihexosylceramide + H2O
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-deoxy-L-altronojirimycin hydrochloride
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1000fold less effective than 1-deoxygalactonojirimycin
1-deoxygalactonojirimycin
galactostatin bisulfite
myo-inositol
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N-acetyl-D-galactosamine
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n-butyldeoxygalactonojirimycin
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2000fold less effective than 1-deoxygalactonojirimycin
sodium taurocholate
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additional information
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not inhibited by 2,6-dithiopurine
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-deoxygalactonojirimycin
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.9 - 6.8
4-methylumbelliferyl alpha-D-galactopyranoside
2.2 - 4.5
4-methylumbelliferyl alpha-D-galactoside
1.8 - 16.3
4-methylumbelliferyl-alpha-D-galactoside
0.28
Galabiosylceramide
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3.7
globopentaose
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9.1
globotriose
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-
0.18
globtriglycosylceramide
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-
8.8
o-nitrophenyl alpha-D-fucopyranoside
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1.3
o-nitrophenyl-alpha-N-acetyl-galactosaminide
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alpha-galactosidase B
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.038 - 0.047
1-deoxygalactonojirimycin
0.095 - 0.122
galactostatin bisulfite
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000048 - 0.000063
1-deoxygalactonojirimycin
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.052
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17.6
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2060000
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placental alpha-galactosidase A
229000
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plasma alpha-galactosidase A
4070000
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spleen alpha-galactosidase A
6.56
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74.62
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purified recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 4.5
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4.2
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wild-type and mutant enzymes S65T, S65A and S65D
4.3
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4-methylumbelliferyl-alpha-D-galactopyranoside as substrate
4.8