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IUBMB Comments The enzyme is involved in the hydrolysis of the steroid saponin dioscin by the digestive system of Sus scrofa (pig). cf . EC 3.2.1.189 , dioscin glycosidase (diosgenin-forming).
The expected taxonomic range for this enzyme is: Sus scrofa
Synonyms dioscin alpha-L-rhamnosidase, more
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dioscin alpha-L-rhamnosidase
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3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O = 2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside
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3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin rhamnosidase (3-O-beta-D-Glc-diosgenin-forming)
The enzyme is involved in the hydrolysis of the steroid saponin dioscin by the digestive system of Sus scrofa (pig). cf. EC 3.2.1.189, dioscin glycosidase (diosgenin-forming).
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3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O
2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside
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Substrates: the enzyme is involved in hydrolysis of the steroid saponin dioscin Products: -
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3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O
2 L-rhamnopyranose + diosgenin-3-O-beta-D-glucopyranoside
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Substrates: - Products: -
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additional information
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Substrates: no hydrolysis of ginsenoside Re or rutin Products: -
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3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O
2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside
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Substrates: the enzyme is involved in hydrolysis of the steroid saponin dioscin Products: -
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Ca2+
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10-200 mM, 1.13-1.18fold activation
Mg2+
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inhibits at 10-100 mM, slightly activates (109% of the control activity) at 200 mM
Zn2+
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10-200 mM, 1.08-1.1fold activation
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Cu2+
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10 mM, 94% inhibition
Fe3+
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10 mM, 88% inhibition
Mg2+
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inhibits at 10-100 mM, slightly activates at 200 mM
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4 - 9
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pH 4.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity
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32 - 57
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32°C: about 85% of maximal activity, 57°C: about 35% of maximal activity
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47000
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x * 47000, SDS-PAGE
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Qian, S.; Yu, H.; Zhang, C.; Lu, M.; Wang, H.; Jin, F.
Purification and characterization of dioscin-alpha-L-rhamnosidase from pig liver
Chem. Pharm. Bull.
53
911-914
2005
Sus scrofa
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