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Information on EC 3.2.1.18 - exo-alpha-sialidase
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EC Tree
3.2.1.18 exo-alpha-sialidaseIUBMB Comments
The enzyme does not act on 4-O-acetylated sialic acids. endo-alpha-Sialidase activity is listed as EC 3.2.1.129, endo-alpha-sialidase. See also EC 4.2.2.15 anhydrosialidase.
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Word Map
- 3.2.1.18
- influenza
-
sialic
- viruses
- vaccine
- oseltamivir
-
pandemic
-
avian
- lectin
- subtype
- oligosaccharide
-
sialylated
- lymphocyte
- epitope
- gangliosides
-
season
- trypsin
- erythrocyte
- glycoconjugates
- glycans
- trypanosoma
- hn
- galactose
- chicken
-
outbreak
-
reassortant
-
desialylation
-
hemagglutination
- cholera
-
n-linked
- virion
- cruzi
- parainfluenza
-
n-acetylneuraminic
- perfringens
- mucin
- bird
-
concanavalin
-
surveillance
-
endoglycosidase
- peanut
- pronase
- newcastle
-
agglutination
-
epidemic
-
poultry
- nucleoprotein
- analysis
-
sendai
- n-acetylgalactosamine
- gm3
- ferret
- medicine
- molecular biology
- diagnostics
- nutrition
The enzyme appears in viruses and cellular organisms
Synonyms
acetylneuraminidase, Acetylneuraminyl hydrolase, acid sialidase, acylneuraminyl glycohydrolase, alpha-N-acylneuraminate glycohydrolase, alpha-neuraminidase, alpha-sialidase, alpha2,6-sialidase, alpha2,6-sialyltransferase, alpha2,6-trans-sialidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylneuraminidase
-
-
-
-
Acetylneuraminyl hydrolase
acid sialidase
acylneuraminyl glycohydrolase
-
-
-
-
alpha-neuraminidase
-
-
-
-
Am0705
Am0707
Am1757
Am2085
Cytosolic sialidase
DELTA15Pd2,6ST
-
the enzyme has additional alpha2,6-trans-sialidase and alpha2,6-sialidase activities
G9 sialidase
Ganglioside sialidase
ganglioside-specific sialidase
GH33C
hemagglutinin-neuraminidase
HN
KDNase
Lysosomal sialidase
Major 85 kDa surface antigen
-
-
-
-
Major surface antigen
-
-
-
-
Membrane sialidase
Mouse skeletal muscle sialidase
-
-
-
-
MSS
-
-
-
-
MTS
-
-
-
-
mucopolysaccharide N-acetylneuraminylhydrolase
-
-
-
-
Murine thymic sialidase
-
-
-
-
N-acetylneuraminosyl glycohydrolase
N-acylneuraminate glycohydrolase
-
-
-
-
N-acylneuraminosyl glycohydrolase
NA
NanA
NANase
NanH
NanI
NanJ
Neu
NEU1
NEU2
Neu3
NEU4
neuraminidase
neuraminidase 1
SA85-1.1 protein
-
-
-
-
SA85-1.2 protein
-
-
-
-
SA85-1.3 protein
-
-
-
-
SiaBb1
sialidase
sialyl hydrolase
STNA
-
-
-
-
TDE0471
trans-sialidase
Acetylneuraminyl hydrolase
-
-
-
-
Cytosolic sialidase
-
-
-
-
G9 sialidase
-
-
-
-
Ganglioside sialidase
-
-
-
-
hemagglutinin-neuraminidase
A2THD4, A7L4X3, A7UC53, A7UC55, A7UC57, A7UC61, A7UC62, A7UC63, A7UC64, A7UC67, A7UC69, A7UC70, Q83758, Q914X2, Q9W8Z8
-
Lysosomal sialidase
-
-
-
-
Membrane sialidase
-
-
-
-
NANase
-
-
-
-
neuraminidase
-
-
-
-
neuraminidase
-
-
sialidase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
-
-
-
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
enzyme does not act on 4-O-acetylated sialic acids
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase, the active center contains essential cysteine residues
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase, active center contains essential cysteine residues
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
reaction and kinetic mechanisms
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
active site, Arg498, Glu258, Tyr317, and Tyr262 are catalytic residues, with the aromatic ring of the latter being important for function
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
trans-sialidase preferring the transfer of sialic acids from sialoglycoconjugates to beta-galactosyl acceptor molecules, ping-pong, double diplacement catalytic mechanism, Tyr342 is important, Asp59 is the acid/base residue, enzyme-substrate complex structure, covalent sialyl-enzyme intermediate
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
catalytic chemistry involves the formation of a covalent intermediate with an active site tyrosine nucleophile
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
ping-pong bi-bi kinetic mechanism. Residue D59 functions as acid/base catalyst, and Y342 as active site nucleophile
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
catalytic mechanism, overview
-
hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates
exo-glycosidase
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
acetylneuraminyl hydrolase
The enzyme does not act on 4-O-acetylated sialic acids. endo-alpha-Sialidase activity is listed as EC 3.2.1.129, endo-alpha-sialidase. See also EC 4.2.2.15 anhydrosialidase.
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CAS REGISTRY NUMBER
COMMENTARY
9001-67-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,3)Gal + p-nitrophenol
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
N-glycolylneuraminic acid + 4-nitrophenol + ?
-
-
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)Gal + p-nitrophenol
(N-glycolylneuraminic acid)(alpha2,6)Galbeta-p-nitrophenol + H2O
N-glycolylneuraminic acid + 4-nitrophenol + ?
-
-
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)GalNAc + p-nitrophenol
(trifluoromethyl)umbelliferyl alpha-sialoside + H2O
(trifluoromethyl)umbelliferone + sialic acid
-
-
-
-
?
(trifluoromethyl)umbelliferyl alpha-sialoside + lactose
(trifluoromethyl)umbelliferone + sialyllactose
-
-
-
-
?
2'-(4-methyl-umbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methyl-umbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methyl-umbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methyl-umbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl) 5-glycolyl-alpha-D-N-acetylneuraminic acid + H2O
4-methyl-umbelliferone + 5-glycolyl-alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl) 9-O-acetyl-alpha-D-N-acetylneuraminic acid + H2O
4-methyl-umbelliferone + 9-O-acetyl-alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methyl-umbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
?
-
-
-
-
?
2-(3-methoxyphenyl)-N-acetylneuraminic acid + H2O
3-methoxyphenol + N-acetylneuraminic acid
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetyl-alpha-D-neuraminic acid
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
2-chloro-5-(4-methoxyspiro(1,2-dioxetane-3,29-(5-chloro)tricyclo[3.3.1.13,7]decan)-4-yl-phenyl-5-acetamido-3,5-dideoxy-alpha-D-glycero-D-galacto-2-nonulopyranoside)onate + H2O
?
2-deoxy-2,3-dehydro-N-acetylneuraminic acid + H2O
N-acetylneuraminic acid
-
reaction deduced from crystal structures and confirmed by NMR study
-
?
3'-sialyllactose + Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAc
lactose + Neu5Ac(alpha2,3)[Galp(beta1,2)]Galp(beta1,6)[Galf(beta1,4)]GlcNAc
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
lactose + Neu5Ac(alpha2,3)[Galp(beta1,2)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
-
-
-
-
?
3'-sialyllactose + Galp(beta1,2)[Galp(beta1,3)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
lactose + Neu5Ac(alpha2,3)[Galp(beta1,2)]Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
-
-
-
-
?
3'-sialyllactose + Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAc
?
-
-
-
-
?
3'-sialyllactose + Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcOBn
?
-
-
-
-
?
3'-sialyllactose + Galp(beta1,3)Galp(beta1,6)[Galf(beta1,4)]GlcNAcol
?
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
4-methylumbelliferyl alpha-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl alpha-NeuAc + H2O
4-methylumbelliferone + alpha-neuAc
-
-
-
-
?
4-methylumbelliferyl D-N-acetylneuraminyl-alpha-(2->3)-D-galactopyranoside + H2O
4-methylumbelliferol + N-acetylneuraminate + 4-methylumbelliferyl alpha-D-galactopyranoside
4-methylumbelliferyl N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
4-methylumbelliferyl-3-deoxy-D-glycero-D-galacto-2-nonulosonic acid + H2O
?
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
4-nitrophenyl alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + N-acetylneuraminic acid
-
-
-
?
4-nitrophenyl alpha-sialoside + lactose
4-nitrophenol + sialyllactose
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
5-acetylneuraminic acid-(alpha2,3)Galbeta-4-nitrophenol + H2O
N-acetylneuraminic acid + 4-nitrophenol + ?
-
-
-
-
?
5-acetylneuraminic acid-(alpha2,6)Galbeta-4-nitrophenol + H2O
N-acetylneuraminic acid + 4-nitrophenol + ?
-
-
-
-
?
5-azidoacetylneuraminic acid-(alpha2,3)Galbeta-4-nitrophenol + H2O
N-azidoacetylneuraminic acid + 4-nitrophenol + ?
-
-
-
-
?
5-azidoacetylneuraminic acid-(alpha2,6)Galbeta-4-nitrophenol + H2O
N-azidoacetylneuraminic acid + 4-nitrophenol + ?
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O
5-bromo-4-chloro-3-hydroxyindole + N-acetylneuraminic acid
5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid + H2O
?
5-fluoroacetylneuraminic acid-(alpha2,3)Galbeta-4-nitrophenol + H2O
N-fluoroacetylneuraminic acid + 4-nitrophenol + ?
-
-
-
-
?
5-fluoroacetylneuraminic acid-(alpha2,6)Galbeta-4-nitrophenol + H2O
N-fluoroacetylneuraminic acid + 4-nitrophenol + ?
-
-
-
-
?
8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O
8-fluoromethylumbelliferyl alpha-D-galactopyranoside + 3-deoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonate
alpha(2-6')-sialyllactose + H2O
sialic acid + lactose
the nanB encoded enzyme hydrolyzes both (2-3')- and (2-6')-sialyllactose, the latter substrate is preferred
-
-
?
alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R
?
-
the enzyme is selective for alpha(2-3)-linked sialic acid
-
-
?
Candida albicans cells + H2O
desialylated Candida albicans cells + sialic acid
-
-
-
-
?
Collocalia mucoid + H2O
sialic acid + ?
-
substrate contains a large number of terminal sialic acids
-
-
?
ganglioside GD3 + H2O
N-acetylneuraminate + ganglioside GM3
-
100% activity
-
-
?
ganglioside GM3 + H2O
N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosylceramide
ganglioside GM3(Neu5Ac) + H2O
desialylated ganglioside GM3 + sialic acid
-
i.e. Neu5Acalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer
-
-
?
ganglioside GM3(Neu5Gc) + H2O
desialylated ganglioside GM3 + sialic acid
-
i.e. Neu5Gcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer
-
-
?
ganglioside IV3-alpha-Neu5Ac-nLc4Cer + H2O
desialylated ganglioside nLc4Cer + sialic acid
-
i.e. Neu5Acalpha(2-3)-Galbeta(1-4)GcNAcbeta(1-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer
-
-
?
ganglioside VI3-alpha-Neu5Ac-nLc6Cer + H2O
desialylated ganglioside nLc6Cer + sialic acid
-
i.e. Neu5Acalpha(2-3)-Galbeta(1-4)GcNAcbeta(1-3)-Galbeta(1-4)-GlcNAcbeta(1-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer
-
-
?
methyl beta-lactoside + p-nitrophenyl-5,9-diacetyl neuraminic acid
?
-
-
-
-
?
methyl beta-lactoside + p-nitrophenyl-N-acetyl heptulosonic acid
?
-
-
-
-
?
methyl beta-lactoside + p-nitrophenyl-N-acetyl octulosonic acid
?
-
-
-
-
?
N-(2,4,7,8,9-penta-O-acetyl-N-acetyl-beta-D-neuraminoyl)phenylalanine methylester + H2O
?
-
-
-
-
?
N-(4,7,8,9-tetra-O-acetyl-N-acetyl-2-O-benzyl-alpha-D-neuraminoyl)phenylalanine methylester + H2O
?
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,6-D-galactose + H2O
N-acetylneuraminic acid + D-galactose
-
-
-
-
?
Neu5(4-[(2,4-dinitrophenoxy)methyl]-1H-1,2,3-triazol-1-yl)Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
?
-
-
-
-
?
Neu5,9Ac2(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
Neu5,9Ac2(alpha2,6)GalNAc + p-nitrophenol
Neu5,9Ac2alpha2,6Galbeta-p-nitrophenol + H2O
Neu5,9Ac2alpha2,6Gal + p-nitrophenol
-
low activity
-
-
?
Neu5Ac(alpha2,3)Galbeta-p-nitrophenol + H2O
Neu5Acalpha2,3Gal + p-nitrophenol
-
-
-
-
?
Neu5Ac-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-CH2(CH3)CH2CH2CH3 + H2O
?
-
-
-
-
?
Neu5Ac-alpha-(2->3)-Gal-beta-4-nitrophenol + H2O
Neu5Ac + 4-nitrophenyl beta-D-galactopyranoside
-
-
-
-
?
Neu5Ac-alpha-(2->6)-Gal-beta-4-nitrophenol + H2O
Neu5Ac + 4-nitrophenyl beta-D-galactopyranoside
-
-
-
-
?
Neu5Ac9(2,4-dinitrobenzoyl)N-alpha-(2->3)-Gal-beta--(1->4)-Glc-beta-n-octyl + H2O
?
-
-
-
-
?
Neu5Acalpha2,6Galbeta-p-nitrophenol + H2O
Neu5Acalpha2,6Galbeta + p-nitrophenol
-
-
-
-
?
Neu5AcF-alpha-(2->3)-Gal-beta-4-nitrophenol + H2O
Neu5AcF + 4-nitrophenyl beta-D-galactopyranoside
-
-
-
-
?
Neu5glycolyl-alpha-(2->3)-Gal-beta-4-nitrophenol + H2O
N-glycolylneuraminic acid + 4-nitrophenyl beta-D-galactopyranoside
-
-
-
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3,4-dimethylpyridinium + H2O
sialic acid + 3,4-dimethylpyridine
-
-
-
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-3-methylpyridinium + H2O
sialic acid + 3-methylpyridine
-
-
-
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]-4-methylpyridinium + H2O
sialic acid + 4-methylpyridine
-
-
-
-
?
N[(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosyl)onate]pyridinium + H2O
sialic acid + pyridine
-
-
-
-
?
O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galactonon-2-ulopyranosylonic acid)-(2->3)-O-(beta-D-galactopyranosyl)-(1->4)-O-(beta-D-glucopyranosyl)-octanol + H2O
?
-
-
-
-
?
O-(5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galactonon-2-ulopyranosylonic acid)-(2->6)-O-(beta-D-galactopyranosyl)-(1->4)-O-(beta-D-glucopyranosyl)-octanol + H2O
?
-
-
-
-
?
O-(5-acetamido-9-acetoxy-3,5,9-trideoxy-D-glycero-alpha-D-galactonon-2-ulopyranosylonic acid)-(2->3)-O-(beta-D-galactopyranosyl)-(1->4)-O-(beta-D-glucopyranosyl)-octanol + H2O
?
-
-
-
-
?
O-(5-acetamido-9-acetoxy-3,5,9-trideoxy-D-glycero-alpha-D-galactonon-2-ulopyranosylonic acid)-(2->6)-O-(beta-D-galactopyranosyl)-(1->4)-O-(beta-D-glucopyranosyl)-octanol + H2O
?
-
-
-
-
?
O-(5-glycolylamido-3,5,-dideoxy-D-glycero-alpha-D-galactonon-2-ulopyranosylonic acid)-(2->3)-O-(beta-D-galactopyranosyl)-(1->4)-O-(beta-D-glucopyranosyl)-octanol + H2O
?
-
-
-
-
?
O-(5-glycolylamido-3,5,-dideoxy-D-glycero-alpha-D-galactonon-2-ulopyranosylonic acid)-(2->6)-O-(beta-D-galactopyranosyl)-(1-> 4)-O-(beta-D-glucopyranosyl)-octanol + H2O
?
-
-
-
-
?
phenyl beta-sialoside + H2O
phenol + alpha-sialic acid
mutant enzymes Y370G, Y370A, Y370N and Y370T catalyze the hydrolysis of phenyl beta-sialoside with an inversion of the anomeric configuration. Mutant enzyme Y370A shows 4% of the activity of Y370G, mutant enzymes Y370N and Y370T show 9% of the activity of Y370G
-
-
?
red blood ghost cells + H2O
?
-
desialylation, preference for different species: in descending order mouse, rat, camel, goat, and dog
-
-
?
sialic acid + lactose
6'-sialyllactose
the enzyme is able to transfer sialyl from sialic acid dimer or oligomer to lactose with high efficiency and strict alpha2-6 regioselectivity
-
-
r
sialic acid tetramer with alpha-linked trifluoromethylumbelliferyl + H2O
?
-
hydrolysis with formation of two alpha-linked trifluoromethylumbelliferyl glycoside-containing species, trifluoromethylumbelliferone and the TFMU sialoside monomer
-
-
?
sialic acid trimer with alpha-linked trifluoromethylumbelliferyl + H2O
?
-
degraded with exclusive cleavage at the coumarin-sialoside bond
-
-
?
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,3)Gal + p-nitrophenol
-
-
-
-
?
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,3)Gal + p-nitrophenol
-
weak activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,3)Gal + p-nitrophenol
-
-
-
-
?
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,3)Gal + p-nitrophenol
-
-
-
-
?
(N-glycolylneuraminic acid)(alpha2,3)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,3)Gal + p-nitrophenol
-
-
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)Gal + p-nitrophenol
-
low activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)Gal + p-nitrophenol
-
weak activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)Galbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)Gal + p-nitrophenol
-
low activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)GalNAc + p-nitrophenol
-
low activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)GalNAc + p-nitrophenol
-
weak activity
-
-
?
(N-glycolylneuraminic acid)(alpha2,6)GalNAcbeta-p-nitrophenol + H2O
(N-glycolylneuraminic acid)(alpha2,6)GalNAc + p-nitrophenol
-
low activity
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl) alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
2-(3-methoxyphenyl)-N-acetylneuraminic acid + H2O
3-methoxyphenol + N-acetylneuraminic acid
-
-
-
-
?
2-(3-methoxyphenyl)-N-acetylneuraminic acid + H2O
3-methoxyphenol + N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
synthetic, fluorogenic substrate
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetyl-alpha-D-neuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetyl-alpha-D-neuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetyl-alpha-D-neuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-(4-nitrophenyl)-alpha-D-N-acetylneuraminic acid + H2O
4-nitrophenol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
2-chloro-5-(4-methoxyspiro(1,2-dioxetane-3,29-(5-chloro)tricyclo[3.3.1.13,7]decan)-4-yl-phenyl-5-acetamido-3,5-dideoxy-alpha-D-glycero-D-galacto-2-nonulopyranoside)onate + H2O
?
-
-
-
-
?
2-chloro-5-(4-methoxyspiro(1,2-dioxetane-3,29-(5-chloro)tricyclo[3.3.1.13,7]decan)-4-yl-phenyl-5-acetamido-3,5-dideoxy-alpha-D-glycero-D-galacto-2-nonulopyranoside)onate + H2O
?
-
-
-
-
?
2-chloro-5-(4-methoxyspiro(1,2-dioxetane-3,29-(5-chloro)tricyclo[3.3.1.13,7]decan)-4-yl-phenyl-5-acetamido-3,5-dideoxy-alpha-D-glycero-D-galacto-2-nonulopyranoside)onate + H2O
?
-
-
-
-
?
2-chloro-5-(4-methoxyspiro(1,2-dioxetane-3,29-(5-chloro)tricyclo[3.3.1.13,7]decan)-4-yl-phenyl-5-acetamido-3,5-dideoxy-alpha-D-glycero-D-galacto-2-nonulopyranoside)onate + H2O
?
-
-
-
-
?
2-chloro-5-(4-methoxyspiro(1,2-dioxetane-3,29-(5-chloro)tricyclo[3.3.1.13,7]decan)-4-yl-phenyl-5-acetamido-3,5-dideoxy-alpha-D-glycero-D-galacto-2-nonulopyranoside)onate + H2O
?
-
-
-
-
?
3'-sialyllactose + H2O
sialic acid + lactose
-
-
-
?
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid + H2O
?
preferred substrate
-
-
?
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid + H2O
?
preferred substrate
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
about 1% activity compared to ganglioside GD3
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
influenza A virus H1N1 pdm09
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
?
-
-
-
?
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
?
-
-
-
?
4-methylumbelliferyl alpha-sialoside + H2O
?
48.9% activity compared to alpha(2-6)-sialyllactose
-
-
?
4-methylumbelliferyl alpha-sialoside + H2O
?
48.9% activity compared to alpha(2-6)-sialyllactose
-
-
?
4-methylumbelliferyl D-N-acetylneuraminyl-alpha-(2->3)-D-galactopyranoside + H2O
4-methylumbelliferol + N-acetylneuraminate + 4-methylumbelliferyl alpha-D-galactopyranoside
-
preference for the alpha2-3 isomer of 4-methylumbelliferyl alpha-D-N-acetylneuraminylgalactopyranoside
-
-
?
4-methylumbelliferyl D-N-acetylneuraminyl-alpha-(2->3)-D-galactopyranoside + H2O
4-methylumbelliferol + N-acetylneuraminate + 4-methylumbelliferyl alpha-D-galactopyranoside
-
preference for the alpha2-3 isomer of 4-methylumbelliferyl alpha-D-N-acetylneuraminylgalactopyranoside
-
-
?
4-methylumbelliferyl N-acetyl-alpha-D-neuraminic acid + H2O
?
highest activity
-
-
?
4-methylumbelliferyl N-acetyl-alpha-D-neuraminic acid + H2O
?
highest activity
-
-
?
4-methylumbelliferyl N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
unidentified influenza virus A/Quebec/144147/09
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
isozyme Neu-1 and Neu-3
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
H7N9 subtype A/Anhui/1/2013
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
H7N9 subtype A/Shanghai/1/2013
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
L0N7M7
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
4-methylumbelliferone + N-acetylneuraminic acid
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
-
?
4-nitrophenyl alpha-sialoside + H2O
?
65.5% activity compared to alpha(2-6)-sialyllactose
-
-
?
4-nitrophenyl alpha-sialoside + H2O
?
65.5% activity compared to alpha(2-6)-sialyllactose
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-5-fluoro-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
about 30% cleavage
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
about 5% cleavage
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(3-deoxy-5-O-methyl-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-(5-azido-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid)-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
less than 10% cleavage
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
less than 5% cleavage
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-azidoacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
about 50% cleavage
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
about 35% cleavage
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-fluoroacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-3)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
4-nitrophenyl O-[5-(2-methoxyacetamido)-3,5-dideoxy-D-glycero-alpha-D-galacto-2-nonulopyranosylonic acid]-(2-6)-O-beta-D-galactopyranoside + H2O
?
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O
5-bromo-4-chloro-3-hydroxyindole + N-acetylneuraminic acid
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O
5-bromo-4-chloro-3-hydroxyindole + N-acetylneuraminic acid
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O
?
-
-
-
-
?
5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid + H2O
?
-
-
-
-
?
5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid + H2O
?
-
-
-
-
?
5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid + H2O
?
Akkermansia muciniphila DSM 22959
-
-
-
-
?
6'-sialyllactose + H2O
sialic acid + lactose
-
-
-
?
6'-sialyllactose + H2O
sialic acid + lactose
isoform Neu3a shows low activity
-
-
?
8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O
8-fluoromethylumbelliferyl alpha-D-galactopyranoside + 3-deoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonate
-
-
-
?
8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O
8-fluoromethylumbelliferyl alpha-D-galactopyranoside + 3-deoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonate
-
-
-
?
alpha(2-3')-sialyllactose + H2O
sialic acid + lactose
nanH encoded enzyme is highly specific for the (2-3')-sialyllactose
-
-
?
alpha(2-3')-sialyllactose + H2O
sialic acid + lactose
the nanB encoded enzyme hydrolyzes both 2-3'- and 2-6'-sialyllactose
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
89.8% activity compared to alpha(2-6)-sialyllactose
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
89.8% activity compared to alpha(2-6)-sialyllactose
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
-
preference for alpha(2-3)-linkages
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
-
high activity
-
-
?
alpha(2-3)-sialyllactose + H2O
sialic acid + lactose
-
formation of a covalent sialyl-enzyme intermediate
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
100% activity
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
100% activity
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
-
low activity
-
-
?
alpha(2-6)-sialyllactose + H2O
sialic acid + lactose
-
DELTA15Pd2,6ST-catalyzed trans-sialidase reactions follow a ping-pong bi-bi reaction mechanism, DELTA15Pd2,6ST preferentially cleaves alpha2,6-linked sialic acid
-
-
?
alpha-sialyllactose + H2O
sialic acid + lactose
-
-
-
-
?
bovine submaxillary gland mucin + H2O
sialic acid + ?
-
-
-
?
colominic acid + H2O
sialic acid + lactose
i.e. (2-8')-sialyllactose
-
-
?
fetuin + H2O
?
isoform Neu3a shows low activity
-
-
?
ganglioside GD1a + H2O
ganglioside GM1 + sialic acid
-
i.e. NeuAcalpha(2-3)-Galbeta(1-3)-GalNAcbeta(1-4)-NeuAcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer, isozyme Neu-3, no activity with Neu-1
-
-
?
ganglioside GD1a + H2O
ganglioside GM1 + sialic acid
-
i.e. NeuAcalpha(2-3)-Galbeta(1-3)-GalNAcbeta(1-4)-NeuAcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer, isozyme Neu-3, no activity with Neu-1
-
-
?
ganglioside GD1a + H2O
ganglioside GM1 + sialic acid
-
in transfected cells, mainly via cell-cell-interaction
-
-
?
ganglioside GD3 + H2O
?
isoform Neu3a shows highest activity with ganglioside GD3
-
-
?
ganglioside GM3 + H2O
N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosylceramide
-
-
-
-
?
ganglioside GM3 + H2O
N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosylceramide
-
about 60% activity compared to ganglioside GD3
-
-
?
ganglioside GM3 + H2O
sialic acid + ?
-
in transfected cells, mainly via cell-cell-interaction
-
-
?
mucin of bovine submaxillary gland + H2O
sialic acid + ?
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,3-lactose + H2O
N-acetylneuraminic acid + lactose
-
-
-
-
?
N-acetylneuraminic acid-alpha-2,6-lactose + H2O
N-acetylneuraminic acid + lactose