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Information on EC 3.1.4.44 - glycerophosphoinositol glycerophosphodiesterase
for references in articles please use BRENDA:EC3.1.4.44
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EC Tree 3 Hydrolases
3.1 Acting on ester bonds
3.1.4 Phosphoric-diester hydrolases
3.1.4.44 glycerophosphoinositol glycerophosphodiesterase
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
- 3.1.4.44
- glycerophosphocholine
- dicer
-
au-rich
-
mir15a
-
let-7a
-
forensically
-
fluid-specific
Reaction Schemes
showSynonyms
mir16, gde1/mir16, glycerophosphodiester phosphodiesterase domain containing 5, glycerophosphoinositol phosphodiesterase, gpi-pde, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
GDE1
glycerophosphodiester phosphodiesterase domain containing 5
GDPD5, BC024955
glycerophosphoinositol glycerophosphohydrolase
-
-
-
-
phosphodiesterase, glycerophosphoinositol
-
-
-
-
sn-glycero(3)phosphoinositol glycerophosphohydrolase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1-(sn-glycero-3-phospho)-1D-myo-inositol + H2O = myo-inositol + sn-glycerol 3-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
hydrolysis of phosphoric ester
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
1-(sn-glycero-3-phospho)-1D-myo-inositol glycerophosphohydrolase
-
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CAS REGISTRY NUMBER
COMMENTARY
72414-13-2
not distinguished from EC 3.1.4.43
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-(sn-glycero-3-phospho)-1D-myo-inositol + H2O
myo-inositol + sn-glycerol 3-phosphate
-
Substrates: selectively hydrolyzes glycerophosphoinositol over glycerophosphocholine
Products: -
Products: -
?
glycerophosphocholine + H2O
choline + glycerolphosphate
-
Substrates: selectively hydrolyzes glycerophosphoinositol over glycerophosphocholine
Products: -
Products: -
?
glycerophosphoinositol + H2O
glycerol 3-phosphate + inositol
Substrates: -
Products: -
Products: -
?
glycerophosphoinositol + H2O
glycerol 3-phosphate + inositol
-
Substrates: enzyme selectively hydrolyzes glycerophosphoinositol
Products: -
Products: -
?
sn-glycero-3-phospho-1-D-myo-inositol + H2O
sn-glycero 3-phosphate + inositol
-
Substrates: -
Products: -
Products: -
?
sn-glycero-3-phospho-1-D-myo-inositol + H2O
sn-glycero 3-phosphate + inositol
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme might cleave teichoic acid and lipoteichoic acid at C1 and C3 of their glycerophosphates, respectively
Products: -
Products: -
?
additional information
?
-
-
Substrates: glycerophosphoinositol 4-phosphate and glycerophosphoinositol 4,5-diphosphate are potential substrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: no hydrolysis of glycerophosphocholine
Products: -
Products: -
?
additional information
?
-
-
Substrates: regulation of GDE1 activity by G protein signalling, GDE1 provides a link between phosphoinositide metabolism and G protein signal transduction, GDE1 is most likely involved in the remodeling of membrane phosphoinositides and regulation of membrane phospholipid composition, and hence in intracellular membrane trafficking
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
Substrates: regulation of GDE1 activity by G protein signalling, GDE1 provides a link between phosphoinositide metabolism and G protein signal transduction, GDE1 is most likely involved in the remodeling of membrane phosphoinositides and regulation of membrane phospholipid composition, and hence in intracellular membrane trafficking
Products: -
Products: -
?
glycerophosphoinositol + H2O
glycerol 3-phosphate + inositol
Substrates: -
Products: -
Products: -
?
glycerophosphoinositol + H2O
glycerol 3-phosphate + inositol
-
Substrates: enzyme selectively hydrolyzes glycerophosphoinositol
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
KCl
in vitro: no direct decrease in GDPD5 activity, measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine
Mg2+
NaCl
in vivo: activity decreases (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no direct decrease in GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high NaCl concentration.
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
glycerophosphoethanolamine
-
blocks the hydrolysis of glycerophosphoinositol by 30%
glycerophosphoinositol 4,5-diphosphate
-
blocks the hydrolysis of glycerophosphoinositol by 70%
Glycerophosphoinositol 4-phosphate
-
blocks the hydrolysis of glycerophosphoinositol by 30%
glycerophosphoserine
-
blocks the hydrolysis of glycerophosphoinositol by 80%
Urea
in vivo: activity decreases in vivo (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no directl inhibition of GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high urea concentration.
additional information
-
GDE1 activity expressed in HEK 293T cells can be regulated by stimulation of G protein-coupled, alpha/beta-adrenergic and lysophospholipid receptors, phenylephrine reduces the GDE1 activity by 30% and lysophatidic acid by 40% compared with untreated cells
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
GDE1 activity expressed in HEK 293T cells can be regulated by stimulation of G protein-coupled, alpha/beta-adrenergic and lysophospholipid receptors, isoproterenol increases the GDE1 activity by 50% compared with untreated cells
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Breast Neoplasms
Glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) expression correlates with malignant choline phospholipid metabolite profiles in human breast cancer.
Diabetes Mellitus, Type 1
miR15a and miR16 in Chilean type 1 diabetes patients: possible association with apoptosis, inflammatory, or autoimmunity markers.
Echinococcosis
MicroRNA profile in immune response of alveolar and cystic echinococcosis patients.
Foot-and-Mouth Disease
Autophagic degradation of SQSTM1 inhibits ovarian cancer motility by decreasing DICER1 and AGO2 to induce MIRLET7A-3P.
Glioblastoma
Differential expression of miR16 in glioblastoma and glioblastoma stem cells: their correlation with proliferation, differentiation, metastasis and prognosis.
Hepatitis C
The potential regulatory role of miR16 to the interplay between interferon and transforming growth factor beta pathways through IRF3 and SMAD7 in hepatitis C virus infected patients.
Infections
The potential regulatory role of miR16 to the interplay between interferon and transforming growth factor beta pathways through IRF3 and SMAD7 in hepatitis C virus infected patients.
Leukemia, Lymphocytic, Chronic, B-Cell
Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.
Lymphoma
Differential expression of miR16 in glioblastoma and glioblastoma stem cells: their correlation with proliferation, differentiation, metastasis and prognosis.
Neoplasm Metastasis
Differential expression of miR16 in glioblastoma and glioblastoma stem cells: their correlation with proliferation, differentiation, metastasis and prognosis.
Neoplasm Metastasis
GDPD5, a target of miR-195-5p, is associated with metastasis and chemoresistance in colorectal cancer.
Neoplasms
Differential expression of miR16 in glioblastoma and glioblastoma stem cells: their correlation with proliferation, differentiation, metastasis and prognosis.
Neoplasms
Glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) expression correlates with malignant choline phospholipid metabolite profiles in human breast cancer.
Neoplasms
In vivo multimodality imaging of miRNA-16 iron nanoparticle reversing drug resistance to chemotherapy in a mouse gastric cancer model.
Neoplasms
Methyl(R217)HuR and MCM6 are inversely correlated and are prognostic markers in non small cell lung carcinoma.
Ovarian Neoplasms
Oleuropein reduces cisplatin resistance in ovarian cancer by targeting apoptotic pathway regulators.
Pulmonary Disease, Chronic Obstructive
A systematic review of smoking-related epigenetic alterations.
Stomach Neoplasms
In vivo multimodality imaging of miRNA-16 iron nanoparticle reversing drug resistance to chemotherapy in a mouse gastric cancer model.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
integral membrane protein, the catalytic GDE domain faces the lumen/extracellular space and the C-terminus faces the cytoplasm
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
drug target
the enzyme exhibits considerable potential as a novel therapeutic target for non-mucin-producing colon adenocarcinoma
physiological function
the enzyme (GDE1) may be a cancer suppressor by up-regulating the inositol metabolism pathway
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A2K5EEP4_AOTNA
331
0
37532
TrEMBL
other Location (Reliability: 1)
A0A2K6C2V0_MACNE
331
0
37722
TrEMBL
Secretory Pathway (Reliability: 2)
A0A2K6U0P6_SAIBB
331
0
37600
TrEMBL
-
A0A2R9AFK3_PANPA
331
0
37695
TrEMBL
other Location (Reliability: 3)
A0A2U3WMV5_ODORO
331
0
37701
TrEMBL
other Location (Reliability: 1)
A0A341BCB9_NEOAA
331
0
37655
TrEMBL
-
A0A6B0RQ22_9CETA
331
0
37653
TrEMBL
other Location (Reliability: 5)
A0A6P3HA51_BISBB
331
0
37653
TrEMBL
other Location (Reliability: 3)
A0A6P5DZE2_BOSIN
331
0
37680
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6P6HAG3_PUMCO
331
0
37751
TrEMBL
Mitochondrion (Reliability: 2)
A0A811Z5P2_NYCPR
331
0
37732
TrEMBL
Secretory Pathway (Reliability: 1)
A0A8C3W3J5_9CETA
331
0
37633
TrEMBL
other Location (Reliability: 2)
A0A8C6B641_MONMO
331
0
37673
TrEMBL
other Location (Reliability: 1)
A0A8C7BLP0_NEOVI
331
0
37629
TrEMBL
other Location (Reliability: 4)
A0A8D2JRF2_SCIVU
331
0
37760
TrEMBL
other Location (Reliability: 2)
B2R7B1_HUMAN
331
0
37732
TrEMBL
other Location (Reliability: 1)
H0X1U1_OTOGA
331
0
37736
TrEMBL
Secretory Pathway (Reliability: 4)
M1ES98_MUSPF
330
0
37482
TrEMBL
other Location (Reliability: 2)
M3YPL0_MUSPF
331
0
37629
TrEMBL
other Location (Reliability: 3)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
several potential phosphorylation sites detected, possible regulation by kinase phosphorylation
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in mIMCD-3 cell: GDPD5 expression reduces (mRNA level) when osmolarity is increased from 300 to 700 mosmol/kg by adding NaCl (200 mosmol/kg) and urea (200 mosmol/kg) in combination. HEK293 cells transfected with GDPD5: recombinant GDPD5 is perinuclear. GDPD5 colocalizes with neuropathy target esterase in the ER of HEK293 cells. Expression of GDPD5 increases GPC-PDE activity (specific siRNA assay to knock down GDPD5, mIMCD-3 cells)
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
diagnostics
high association with non-mucin-producing colon adenocarcinoma prognosis
medicine
high association with non-mucin-producing colon adenocarcinoma prognosis
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dawson, R.M.C.; Hemington, N.; Richards, D.E.; Irvine, R.F.
sn-Glycero(3)phosphoinositol glycerophosphohydrolase. A new phosphodiesterase in rat tissues
Biochem. J.
182
39-45
1979
Rattus norvegicus
brenda
Kusser, W.; Fiedler, F.
A novel glycerophosphodiesterase from Bacillus pumilus
FEBS Lett.
166
301-306
1984
Bacillus pumilus
brenda
Zheng, B.; Berrie, C.P.; Corda, D.; Farquhar, M.G.
GDE1/MIR16 is a glycerophosphoinositol phosphodiesterase regulated by stimulation of G protein-coupled receptors
Proc. Natl. Acad. Sci. USA
100
1745-1750
2003
Rattus norvegicus
brenda
Yanaka, N.; Nogusa, Y.; Fujioka, Y.; Yamashita, Y.; Kato, N.
Involvement of membrane protein GDE2 in retinoic acid-induced neurite formation in Neuro2A cells
FEBS Lett.
581
712-718
2007
Homo sapiens (Q9NZC3), Mus musculus
brenda
Gallazzini, M.; Ferraris, J.D.; Burg, M.B.
GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC
Proc. Natl. Acad. Sci. USA
105
11026-11031
2008
Mus musculus (Q640M6)
brenda
Shen, Q.; Lu, C.; Yang, H.; Ge, M.X.; Xia, W.X.; Kong, Q.P.; Li, G.H.; Gu, Y.H.
Glycerophosphodiester phosphodiesterase 1 (GDE1) acts as a potential tumor suppressor and is a novel therapeutic target for non-mucin-producing colon adenocarcinoma
PeerJ
8
e8421
2020
Homo sapiens (Q9NZC3)
brenda
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