Information on EC 3.1.3.48 - protein-tyrosine-phosphatase and Organism(s) Bos taurus

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The taxonomic range for the selected organisms is: Bos taurus

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.48
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RECOMMENDED NAME
GeneOntology No.
protein-tyrosine-phosphatase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
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SYSTEMATIC NAME
IUBMB Comments
protein-tyrosine-phosphate phosphohydrolase
Dephosphorylates O-phosphotyrosine groups in phosphoproteins, such as the products of EC 2.7.10.2, non-specific protein-tyrosine kinase.
CAS REGISTRY NUMBER
COMMENTARY hide
79747-53-8
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97162-86-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-naphthyl phosphate + H2O
1-naphthol + phosphate
show the reaction diagram
-
89.3% of the activity with 4-nitrophenyl phosphate, lung enzyme
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
FMN + H2O
?
show the reaction diagram
-
lung enzyme
-
-
-
FMN + H2O
? + phosphate
show the reaction diagram
-
71.8% of the activity with 4-nitrophenyl phosphate, lung enzyme
-
-
?
phosphotyrosine + H2O
tyrosine + phosphate
show the reaction diagram
phosphotyrosyl-casein + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
poly(Glu,Tyr) + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
FMN + H2O
?
show the reaction diagram
-
lung enzyme
-
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phosphotyrosine + H2O
tyrosine + phosphate
show the reaction diagram
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lung enzyme
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-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
-
Cu2+
-
lung enzyme
diphosphate
-
-
Fe2+
-
lung enzyme
L-Tartrate
-
no inhibition of lung enzyme
Mn2+
-
-
n-dodecyl trimethylammonium bromide
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pH 7.0, inactivation at concentration 10fold higher than critical micellar concentration
n-hexadecyl trimethylammonium bromide
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pH 7.0, inactivation at concentration 10fold higher than critical micellar concentration
n-tetradecyl trimethylammonium bromide
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pH 7.0, inactivation at concentration 10fold higher than critical micellar concentration
Ni2+
-
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p-chloromercuribenzoate
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lung enzyme
phosphate
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sodium decylsulfate
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at concentration 10fold lower than critical micellar concentration, complete inactivation. Inactivation is independent of pH-value, irreversible and not affected by NaCl. Presence of phosphate protects
sodium dodecylsulfate
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at concentration 10fold lower than critical micellar concentration, complete inactivation. Inactivation is independent of pH-value, irreversible and not affected by NaCl. Presence of phosphate protects
sodium octylsulfate
-
at concentration 10fold lower than critical micellar concentration, complete inactivation. Inactivation is independent of pH-value, irreversible and not affected by NaCl. Presence of phosphate protects
Sodium tetradecylsulfate
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at concentration 10fold lower than critical micellar concentration, complete inactivation. Inactivation is independent of pH-value, irreversible and not affected by NaCl. Presence of phosphate protects
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014 - 0.44
4-nitrophenyl phosphate
0.012 - 0.36
phosphotyrosine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0975
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47
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
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substrate 4-nitrophenyl phosphate
6
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phosphotyrosine
6.5 - 7
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7
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substrate phosphotyrosyl histone
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17800
-
x * 17800, SDS-PAGE
50000
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gel filtration
52000
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1 * 52000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 17800, SDS-PAGE
monomer
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1 * 52000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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20% loss of activity after 30 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very susceptible to proteolytic degradation
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, stable for at least 4 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
separation of seven cytosolic enzymes
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