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Information on EC 3.1.3.45 - 3-deoxy-manno-octulosonate-8-phosphatase
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3.1.3.45 3-deoxy-manno-octulosonate-8-phosphataseSpecify your search results
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase, 3-deoxy-D-manno-octulosonate-8-phosphate phosphatase, 3-deoxy-D-manno-octulosonate-8-phosphate phosphohydrolase, 3-deoxy-manno-octulosonate-8-phosphatase, KDO8P phosphatase, KDO8PP, KdsC, KPP, phosphatase, phospho-3-deoxy-2-octulosonate, yrbI, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase
3-deoxy-D-manno-octulosonate-8-phosphate phosphatase
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-
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3-deoxy-D-manno-octulosonate-8-phosphate phosphohydrolase
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-
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KDO8P phosphatase
KDO8PP
KdsC
phosphatase, phospho-3-deoxy-2-octulosonate
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-
-
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yrbI
2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase
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2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3-deoxy-D-manno-octulosonate 8-phosphate + H2O = 3-deoxy-D-manno-octulosonate + phosphate
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
additional information
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KDO-phosphatase is not essential for viability of Escherichia coli
hydrolysis of phosphoric ester
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-
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-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
3-deoxy-D-manno-octulosonate-8-phosphate 8-phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
59088-24-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
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last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway
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?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
? + phosphate
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-
-
-
?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
? + phosphate
-
best substrate
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-
?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
? + phosphate
-
-
-
?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
? + phosphate
best substrate
-
-
?
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
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-
-
?
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
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-
-
-
?
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
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high specificity
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?
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
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the enzyme is absolutely specific for the alpha-pyranose anomer
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-
?
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
Escherichia coli B / ATCC 11303
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-
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?
KDN 9-phosphate + H2O
3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate + phosphate
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-
-
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?
KDN 9-phosphate + H2O
3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate + phosphate
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-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
last enzyme of 3-deoxy-D-manno-octulosonate biosynthetic pathway
-
?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
? + phosphate
-
best substrate
-
-
?
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
? + phosphate
best substrate
-
-
?
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
-
-
-
?
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
-
the enzyme is absolutely specific for the alpha-pyranose anomer
-
-
?
3-deoxy-D-manno-octulosonate 8-phosphate + H2O
3-deoxy-D-manno-octulosonate + phosphate
Escherichia coli B / ATCC 11303
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-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ba2+
Co2+
Mg2+
Mn2+
Zn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0001
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate
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at pH 7.0 and 25°C
0.16
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate
at pH 7.0 and 25°C
0.058
3-deoxy-D-manno-octulosonate 8-phosphate
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in presence of 1.0 mM Co2+, 37°C
0.075
3-deoxy-D-manno-octulosonate 8-phosphate
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pH 7.0, 37°C, presence of 1 mM Mg2+
0.091
3-deoxy-D-manno-octulosonate 8-phosphate
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in presence of 1.0 mM Mg2+, 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.78
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate
at pH 7.0 and 25°C
1.5
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate
-
at pH 7.0 and 25°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4.8
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate
at pH 7.0 and 25°C
15
2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate
-
at pH 7.0 and 25°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Show AA Sequence and Transmembrane Helices (7184 entries)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)
Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
tetramer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme bound to Mg2+ and metavanadate, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol MME 550, 40 mM MgCl2 and 100 mM HEPES (pH 7.5), at 18°C
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to 2.25 A resolution, space group P212121. Four protomers of YrbI in the asymmetric unit form a tetramer
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enzyme bound to Mg2+ and metavanadate, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol MME 550, 40 mM MgCl2 and 100 mM HEPES (pH 7.5), at 18°C
structures of KdsC in complex with Mg2+, citrate and products and capturing of transition states of the enzyme. The binding of ligands does not cause any specific conformational changes in the active site. The conformation of substrate 3-deoxy-D-manno-octulosonic acid is different from ist conformation when it binds as a cleaved product. An intersubunit tunnel is observed
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
TALON metal affinity resin column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ray, P.H.; Benedict, C.D.
Purification and characterization of a specific 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase from Escherichia coli B
J. Bacteriol.
142
60-68
1980
Escherichia coli, Escherichia coli B / ATCC 11303
brenda
Baasov, T.; Jakob, A.
Anomeric specificity of 3-deoxy-D-manno-2-octulosonate 8-phosphate phosphatase from Escherichia coli
J. Am. Chem. Soc.
112
4972-4974
1990
Escherichia coli
-
brenda
Wu, J.; Woodard, R.W.
Escherichia coli YrbI is 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase
J. Biol. Chem.
278
18117-18123
2003
Escherichia coli
brenda
Sperandeo, P.; Pozzi, C.; Deho, G.; Polissi, A.
Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus
Res. Microbiol.
157
547-558
2006
Escherichia coli
brenda
Daughtry, K.D.; Huang, H.; Malashkevich, V.; Patskovsky, Y.; Liu, W.; Ramagopal, U.; Sauder, J.M.; Burley, S.K.; Almo, S.C.; Dunaway-Mariano, D.; Allen, K.N.
Structural basis for the divergence of substrate specificity and biological function within HAD phosphatases in lipopolysaccharide and sialic acid biosynthesis
Biochemistry
52
5372-5386
2013
Bacteroides thetaiotaomicron, Haemophilus influenzae, Haemophilus influenzae (P45314)
brenda
Dhindwal, S.; Priyadarshini, P.; Patil, D.N.; Tapas, S.; Kumar, P.; Tomar, S.; Kumar, P.
Ligand-bound structures of 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase from Moraxella catarrhalis reveal a water channel connecting to the active site for the second step of catalysis
Acta Crystallogr. Sect. D
71
239-255
2015
Moraxella catarrhalis (A0A0J9X241), Moraxella catarrhalis BC8 (A0A0J9X241)
brenda
Park, J.; Lee, D.; Kim, M.; Kim, D.; Shin, D.
A preliminary X-ray study of 3-deoxy-D-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI) from Burkholderia pseudomallei
Acta Crystallogr. Sect. F
71
790-793
2015
Burkholderia pseudomallei
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brenda
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