Information on EC 3.1.3.16 - protein-serine/threonine phosphatase and Organism(s) Drosophila melanogaster

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The taxonomic range for the selected organisms is: Drosophila melanogaster

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.16
-
RECOMMENDED NAME
GeneOntology No.
protein-serine/threonine phosphatase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
protein-serine/threonine-phosphate phosphohydrolase
A group of enzymes removing the serine- or threonine-bound phosphate group from a wide range of phosphoproteins, including a number of enzymes that have been phosphorylated under the action of a kinase (cf. EC 3.1.3.48 protein-tyrosine-phosphatase). The spleen enzyme also acts on phenolic phosphates and phosphamides (cf. EC 3.9.1.1, phosphoamidase).
CAS REGISTRY NUMBER
COMMENTARY hide
9025-75-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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PGAM5 is an evolutionarily conserved activator of apoptosis signal-regulating kinase 1
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
very low activity
-
-
?
p38 + H2O
?
show the reaction diagram
-
-
-
-
?
phosphophosphorylase kinase + H2O
phosphorylase kinase + phosphate
show the reaction diagram
-
specific for alpha-subunit
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated Akt1 + H2O
Akt1 + phosphate
show the reaction diagram
-
-
-
-
?
phosphorylated apoptosis signal-regulating kinase 1 + H2O
apoptosis signal-regulating kinase 1 + phosphate
show the reaction diagram
-
PGAM5 acts as a specific protein Ser/Thr phosphatase that activates apoptosis signal-regulating kinase 1 by dephosphorylation of inhibitory sites (phosphorylation of Thr-838), PGAM5 serves as a specialized activator of apoptosis signal-regulating kinase 1
-
-
?
phosphorylated INAD-protein + H2O
INAD-protein + phosphate
show the reaction diagram
-
INAD-protein is inactivation no afterpotential D-protein
-
-
?
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
phosphorylated phosphorylase alpha + H2O
phosphorylase alpha + phosphate
show the reaction diagram
-
-
-
-
?
RRApSVA + H2O
?
show the reaction diagram
-
-
-
-
?
RRApTVA + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphorylated p53 + H2O
p53 + phosphate
show the reaction diagram
-
the B56-containing PP2A acts partly through p53 suppressing apoptosis
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Drosophila melanogaster protein phosphatase inhibitor-2
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IC50: 0.5-1 nM
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okadaic acid
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IC50: 40 nM
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000005 - 0.000001
Drosophila melanogaster protein phosphatase inhibitor-2
Drosophila melanogaster;
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IC50: 0.5-1 nM
-
0.00004
okadaic acid
Drosophila melanogaster;
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IC50: 40 nM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12.08
-
after MonoQ FPLC purification step
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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highest level
Manually annotated by BRENDA team
PDB
SCOP
CATH
UNIPROT
ORGANISM
Drosophila melanogaster;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
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SDS-PAGE
37000
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x * 37000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 37000, SDS-PAGE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Q Sepharose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression with a His tag in Pichia pastoris
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information