Information on EC 3.1.3.16 - protein-serine/threonine phosphatase and Organism(s) Dictyostelium discoideum

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The taxonomic range for the selected organisms is: Dictyostelium discoideum

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.3.16
-
RECOMMENDED NAME
GeneOntology No.
protein-serine/threonine phosphatase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
protein-serine/threonine-phosphate phosphohydrolase
A group of enzymes removing the serine- or threonine-bound phosphate group from a wide range of phosphoproteins, including a number of enzymes that have been phosphorylated under the action of a kinase (cf. EC 3.1.3.48 protein-tyrosine-phosphatase). The spleen enzyme also acts on phenolic phosphates and phosphamides (cf. EC 3.9.1.1, phosphoamidase).
CAS REGISTRY NUMBER
COMMENTARY hide
9025-75-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
phosphocasein + H2O
casein + phosphate
show the reaction diagram
-
-
-
-
?
phosphoproteins + H2O
proteins + phosphate
show the reaction diagram
phosphorylated phosphorylase alpha + H2O
phosphorylase alpha + phosphate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
calcineurin A, strictly dependent on Mn2+ or Mg2+ or Ca2+
Mg2+
-
calcineurin A, strictly dependent on Mn2+ or Mg2+ or Ca2+
Mn2+
-
calcineurin A, strictly dependent on Mn2+ or Mg2+ or Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
Mn2+-stimulated calcineurin A
8.3
-
Mg2+-stimulated calcineurin A
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
B-subunit of phosphatase PP2A
Manually annotated by BRENDA team
A-subunit of phosphatase PP2A
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
phosphatase PP2A, catalytic subunit C, regulatory subunits A and B, 1 * 37000 + 1 * 65000 + 1 * 55000
55000
phosphatase PP2A, catalytic subunit C, regulatory subunits A and B, 1 * 37000 + 1 * 65000 + 1 * 55000
65000
phosphatase PP2A, catalytic subunit C, regulatory subunits A and B, 1 * 37000 + 1 * 65000 + 1 * 55000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
phosphatase PP2A, catalytic subunit C, regulatory subunits A and B, 1 * 37000 + 1 * 65000 + 1 * 55000
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
overexpression in Dictyostelium discoideum
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F269C
increased enzyme activity against phosphorylase alpha and a higher sensitivity to calculin A