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IUBMB Comments The enzyme, characterized from Pseudomonas species, participates in a peptidoglycan salvage pathway.
Synonyms mupP , MurNAc 6P phosphatase, N-acetylmuramic acid 6-phosphate phosphatase,
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N-acetylmuramic acid 6-phosphate phosphatase
mupP
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N-acetylmuramic acid 6-phosphate phosphatase
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N-acetylmuramic acid 6-phosphate phosphatase
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N-acetyl-D-muramate 6-phosphate + H2O = N-acetyl-D-muramate + phosphate
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MetaCyc
peptidoglycan recycling II
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N-acetyl-D-muramate 6-phosphate phosphohydrolase
The enzyme, characterized from Pseudomonas species, participates in a peptidoglycan salvage pathway.
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N-acetyl-D-glucosamine 6-phosphate + H2O
N-acetyl-D-glucosamine + phosphate
Substrates: -
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N-acetyl-D-muramate 6-phosphate + H2O
N-acetyl-D-muramate + phosphate
additional information
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N-acetyl-D-muramate 6-phosphate + H2O
N-acetyl-D-muramate + phosphate
Substrates: -
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N-acetyl-D-muramate 6-phosphate + H2O
N-acetyl-D-muramate + phosphate
Substrates: -
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additional information
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Substrates: no substrates: N-acetyl-D-muramate 1-phosphate, N-acetyl-D-glucosamine 6-phosphate, N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-glucosamine, D-glucose, D-glucosamine 6-phosphate, glucose 6-phosphate
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additional information
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Substrates: neither alpha-1-phosphorylated N-acetyl-D-muramate, N-acetyl-D-glucose, or D-glucose nor glucosamine 6-phosphate or D-glucose 6-phosphate is used as a substrate
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additional information
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Substrates: no substrates: N-acetyl-D-muramate 1-phosphate, N-acetyl-D-glucosamine 6-phosphate, N-acetyl-D-glucosamine 1-phosphate, N-acetyl-D-glucosamine, D-glucose, D-glucosamine 6-phosphate, glucose 6-phosphate
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N-acetyl-D-muramate 6-phosphate + H2O
N-acetyl-D-muramate + phosphate
Substrates: -
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Mg2+
very low activity in absence of Mg2+, 16fold increase in activity in presence of 10 mM Mg2+
Mg2+
10 mM used in assay conditions
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0.31
N-acetyl-D-muramate 6-phosphate
0.31
N-acetyl-D-muramate 6-phosphate
pH 7.6, 22°C
0.31
N-acetyl-D-muramate 6-phosphate
at pH 7.6 and 22°C
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0.88
N-acetyl-D-muramate 6-phosphate
0.88
N-acetyl-D-muramate 6-phosphate
pH 7.6, 22°C
0.88
N-acetyl-D-muramate 6-phosphate
at pH 7.6 and 22°C
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2.84
N-acetyl-D-muramate 6-phosphate
2.84
N-acetyl-D-muramate 6-phosphate
pH 7.6, 22°C
2.84
N-acetyl-D-muramate 6-phosphate
at pH 7.6 and 22°C
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4 - 10
at pH 4.0 and pH 10.0, the enzyme activity is 40 and 60%, respectively, of the maximum
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
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physiological function
enzyme is part of the anabolic recycling pathway of Pseudomonas species and specifically converts MurNAc 6-phosphate to MurNAc. A MupP mutant is highly susceptible to fosfomycin, accumulates large amounts of MurNAc 6-phosphate, and shows lower levels of UDP-MurNAc than wild-type cells
physiological function
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enzyme is part of the anabolic recycling pathway of Pseudomonas species and specifically converts MurNAc 6-phosphate to MurNAc. A MupP mutant is highly susceptible to fosfomycin, accumulates large amounts of MurNAc 6-phosphate, and shows lower levels of UDP-MurNAc than wild-type cells
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x * 25890, calculated, recombinant His-tagged protein
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x * 25890, calculated from amino acid sequence
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x * 25890, calculated, recombinant His-tagged protein
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22
40 min, 10% loss of activity
22 - 45
the recombinant enzyme loses about 10% of its activity at 22°C and 70% at 37°C within 40 min and is completely inactivated within 40 min at 45°C. About 50% of its activity remain at 22°C, and about 85% of its activity remain at 37°C
37
40 min, 70% loss of activity
45
40 min, complete inactivation
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the long-term stability of the enzyme in solution at -80°C is increased by the addition of 20% (v/v) glycerol
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Ni-NTA column chromatography and Superdex 200 gel filtration
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expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
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Borisova, M.; Gisin, J.; Mayer, C.
The N-acetylmuramic acid 6-phosphate phosphatase MupP completes the Pseudomonas peptidoglycan recycling pathway leading to intrinsic fosfomycin resistance
mBio
8
e00092
2017
Pseudomonas putida (Q88M11), Pseudomonas putida DSM 6125 (Q88M11)
brenda
Unsleber, S.; Borisova, M.; Mayer, C.
Enzymatic synthesis and semi-preparative isolation of N-acetylmuramic acid 6-phosphate
Carbohydr. Res.
445
98-103
2017
Pseudomonas putida (Q88M11)
brenda
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