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Information on EC 3.1.3.1 - alkaline phosphatase
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EC Tree
3.1.3.1 alkaline phosphataseIUBMB Comments
Wide specificity. Also catalyses transphosphorylations. The human placental enzyme is a zinc protein. Some enzymes hydrolyse diphosphate (cf. EC 3.6.1.1 inorganic diphosphatase)
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Word Map
- 3.1.3.1
- osteoblast
- osteocalcin
- collagen
-
osteogenic
- aminotransferase
- bilirubin
- mesenchymal
- women
- pancreatitis
- marrow
- albumin
- parathyroid
- urinary
- phosphorus
- creatinine
- count
- arterial
- osteoporosis
- resorption
- cholesterol
- pain
- fracture
- children
-
alt
- necrosis
- transaminase
- spine
- femoral
-
osteogenesis
- bile
- osteopontin
-
lumbar
-
morphogenetic
- anterior
- biliary
- dental
- periodontal
- calcification
- triglyceride
-
postoperative
-
gamma-glutamyl
-
hepatotoxicity
-
preoperative
-
radiological
- transpeptidase
- trabecular
-
admission
- ligament
-
postmenopausal
-
biocompatibility
- agriculture
- biotechnology
- food industry
- molecular biology
- medicine
- diagnostics
- synthesis
- analysis
- environmental protection
The enzyme appears in viruses and cellular organisms
Synonyms
AIP, alkaline p-nitrophenylphosphate phosphatase, alkaline phenyl phosphatase, alkaline phosphatase, alkaline phosphatase 4, alkaline phosphatase F I, alkaline phosphatase F II, alkaline phosphatase HaALP1, alkaline phosphatase HaALP2, alkaline phosphatase HvmALP 1, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alkaline p-nitrophenylphosphate phosphatase
alkaline phenyl phosphatase
-
-
-
-
alkaline phosphatase
alkaline phosphatase 4
alkaline phosphatase IV
alkaline phosphohydrolase
-
-
-
-
alkaline phosphomonoesterase
-
-
-
-
ALP
ALPase
ALPL
alr5291
-
alr5291 encodes a protein that is an atypical alkaline phosphatase like other cyanobacteria PhoAs
AP
AP-TNAP
-
-
-
-
APase
APASED
-
-
-
-
BC6
-
-
-
-
CAPase
EAP
-
-
-
-
Germ-cell alkaline phosphatase
-
-
-
-
glycerophosphatase
-
-
-
-
H-AP
HaALP
High molecular weight phosphatase
-
-
-
-
IAP
IAPase
intestinal alkaline phosphatase
L-AP
Liver/bone/kidney isozyme
-
-
-
-
Low molecular weight phosphatase
-
-
-
-
M-ALP
-
-
-
-
Nagao isozyme
-
-
-
-
non-specific alkaline phosphatase
-
-
-
-
OE5192R
PHOA
phoK
phosphatase, alkaline
-
-
-
-
phosphomonoesterase
-
-
-
-
phox
PLAP
PLAP-like
-
-
-
-
RAN1
-
-
-
-
Regan isozyme
-
-
-
-
SAP
tissue non-specific alkaline phosphatase
tissue-nonspecific alkaline phosphatase
TNAP
TNSALP
ALP
-
-
-
-
APase
-
-
-
-
H-AP
-
-
-
-
IAP
-
-
-
-
L-AP
-
-
-
-
tissue-nonspecific alkaline phosphatase
-
-
TNSALP
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a phosphate monoester + H2O = an alcohol + phosphate
double in-line displacement mechanism involving two-metal ion catalysis
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate, active site structure, highly conserved in evolution of alkaline phosphatases
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate, active site structure, mechanism
shrimp
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate, catalytically active His153 and His328
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate, substrate binding and active site structure
-
a phosphate monoester + H2O = an alcohol + phosphate
Arg166 contributes to catalysis through binding interactions and through additional transition state stabilization that may arise from complementarity of the guanidinum group to the geometry of the trigonal bipyramidal transition state
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
-
a phosphate monoester + H2O = an alcohol + phosphate
Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate
-
-
a phosphate monoester + H2O = an alcohol + phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
phosphomonoester
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
MetaCyc
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SYSTEMATIC NAME
IUBMB Comments
phosphate-monoester phosphohydrolase (alkaline optimum)
Wide specificity. Also catalyses transphosphorylations. The human placental enzyme is a zinc protein. Some enzymes hydrolyse diphosphate (cf. EC 3.6.1.1 inorganic diphosphatase)
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CAS REGISTRY NUMBER
COMMENTARY
9001-78-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-[2-benzthiazole]-6'-hydroxybenzthiazole phosphate + H2O
2'-[2-benzthiazole]-6'-hydroxybenzthiazole + phosphate
-
weakly fluorescent substrate
highly fluorescent product
?
2-chloro-5-(4-methoxyspiro (1,2-dioxetane-3,2'-(5'-chloro)tricyclo[3.3.1.13,7]decan)-4-yl)phenyl phosphate + H2O
2-chloro-5-(4-methoxyspiro (1,2-dioxetane-3,2'-(5'-chloro)tricyclo[3.3.1.13,7]decan)-4-yl)phenol + phosphate
-
CDP-star
-
-
?
3'-ITP + H2O
?
-
51.5% of the activity with 4-nitrophenyl phosphate
-
-
?
4-methoxy-4-(3-phosphatephenyl)spiro [1,2-dioxetane-3,2'-adamantane] disodium salt + H2O
?
-
-
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
-
80% of activity with p-nitrophenyl phosphate
-
-
?
5-bromo-4-chloro-3-indolyl phosphate + H2O
5-bromo-4-chloro-indole + phosphate
Thermus sp. NTU-237
-
-
-
?
5-bromo-4-chloro-3-indolyl phosphate + nitroblue tetrazolium
?
-
i.e. BCIP/NBT system
-
?
adenosine 5'-(beta-glucosyl)diphosphate + H2O
?
-
29.8% of the activity with 4-nitrophenyl phosphate
-
-
?
adenosine 5'-diphosphate + H2O
AMP + phosphate
-
77% of the activity with 4-nitrophenyl phosphate
-
-
?
adenosine 5'-monophosphate + H2O
?
306.1% of the activity with 4-nitrophenyl phosphate
-
-
?
adenosine 5'-triphosphate + H2O
?
257.4% of the activity with 4-nitrophenyl phosphate
-
-
?
adenosine 5'-triphosphate + H2O
AMP + phosphate
-
61% of the activity with 4-nitrophenyl phosphate
-
-
?
alpha-naphthyl acid phosphate + H2O
?
88.6% of the activity with 4-nitrophenyl phosphate
-
-
?
cytidine 5'-monophosphate + H2O
?
343.8% of the activity with 4-nitrophenyl phosphate
-
-
?
D-fructose 1,6-diphosphate + H2O
?
-
52% of the activity with 4-nitrophenyl phosphate
-
-
?
disodium 3-(4-meth- oxyspiro [1,2-dioxetane-3,2'-(5'-chloro)-tricyclo [3.3.1.13,7]- decan]-4-yl)phenyl phosphate + H2O
?
-
-
-
?
guanosine 5'-monophosphate + H2O
?
358.8% of the activity with 4-nitrophenyl phosphate
-
-
?
histone II-A + H2O
?
-
enzyme shows protein phosphatase activity
-
?
indoxyl phosphate + nitroblue tetrazolium chloride
nitrioblue diformazan + phosphate
-
-
-
?
linear DNA fragment + H2O
dephosphorylated linear DNA fragment + phosphate
the enzyme has a dephosphorylation efficiency of 92.9% to dephosphorylate linear DNA fragments of plasmid pUC18
-
-
?
methyl 3-nitrophenyl phosphate + H2O
methyl phosphate + 3-nitrophenol
-
-
-
?
methyl 4-nitrophenyl phosphate + H2O
methyl phosphate + 4-nitrophenol
-
-
-
?
methyl 4-nitrophenyl phosphorothioate + H2O
4-nitrophenol + methyl phosphorothioate
only the R-enantiomer is detectably hydrolyzed by the enzyme
-
-
?
methyl p-nitrophenyl phosphate + H2O
methanol + p-nitrophenol + phosphate
-
-
-
-
?
methyl p-nitrophenyl phosphorothioate + H2O
methanol + p-nitrophenol + phosphorothioate
-
-
-
-
?
N-acetylcysteamine S-phosphate + H2O
N-acetylcysteamine + phosphate
-
-
-
-
?
phosphothreonine + H2O
phosphate + Thr
-
22.1% of the activity with 4-nitrophenyl phosphate
-
-
?
phosphotyrosine + H2O
phosphate + Tyr
-
23.7% of the activity with 4-nitrophenyl phosphate
-
-
?
uridine 5'-monophosphate + H2O
?
388.6% of the activity with 4-nitrophenyl phosphate
-
-
?
[(4-chlorophenyl)thio](10-methyl-9(10H)-acridinylidene)-methanol 1-(dihydrogenphosphate) disodium salt + H2O
?
-
-
-
?
2'-deoxyadenosine 5'-monophosphate + H2O
?
113.3% of the activity with 4-nitrophenyl phosphate
-
-
?
2'-deoxyadenosine 5'-monophosphate + H2O
?
113.3% of the activity with 4-nitrophenyl phosphate
-
-
?
2-phosphoglycerate + H2O
glycerol + phosphate
-
53.7% of the activity with 4-nitrophenyl phosphate
-
-
?
2-phosphoglycerate + H2O
glycerol + phosphate
-
hydrolyzed by isoenzyme III, weak activity with isoenzyme I and II
-
-
?
2-phosphoglycerate + H2O
glycerol + phosphate
-
wild-type activity is 16.9% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 16.3fold higher than activity from wild-type enzyme
-
-
?
3-phosphoglycerate + H2O
glycerol + phosphate
-
hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferone + phosphate
-
-
-
-
?
4-methylumbelliferyl phosphate + H2O
4-methylumbelliferone + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
reaction is at least partially diffusion-controlled
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
non-specific, phosphoseryl intermediate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
strong support for a model in which electrostatic interactions between the bimetallo Zn2+ site and a nonbridging phosphate ester oxygen atom make a significant contribution to the large rate enhancement observed for alkaline phosphatase-catalyzed phosphate monoester hydrolysis
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
hydrolysis of 4-nitrophenylphosphate is nonlinear with time when the enzyme is microinjected into reversed micelles (of hexadecyltrimethylammoniumbromide in cyclohexane, with 1-butanol as cosurfactant) that contain substrate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
hydrolysis of 4-nitrophenylphosphate is nonlinear with time when the enzyme is microinjected into reversed micelles (of hexadecyltrimethylammoniumbromide in cyclohexane, with 1-butanol as cosurfactant) that contain substrate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
allosteric mechanism
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
activity with mutant os-1 enzyme is 12.2fold higher than activity from wild-type enzyme
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
strongest affinity substrate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
best substrate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
non-specific, via phosphoseryl intermediate
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate+ H2O
4-nitrophenol + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
90% of activity with p-nitrophenyl phosphate
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
90% of activity with p-nitrophenyl phosphate
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
80% of activity with p-nitrophenyl phosphate
-
-
?
5'-GMP + H2O
guanosine + phosphate
-
40% of activity with p-nitrophenyl phosphate
-
-
?
5'-UMP + H2O
uridine + phosphate
-
30% of activity with p-nitrophenyl phosphate
-
-
?
5'-UMP + H2O
uridine + phosphate
-
30% of activity with p-nitrophenyl phosphate
-
-
?
5-bromo-4-chloro-3-indolyl phosphate + H2O
?
50.3% of the activity with 4-nitrophenyl phosphate
-
-
?
5-bromo-4-chloro-3-indolyl phosphate + H2O
?
50.3% of the activity with 4-nitrophenyl phosphate
-
-
?
ADP + H2O
AMP + phosphate
-
wild-type activity is 16.9% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 1.2fold lower than activity from wild-type enzyme
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
60% of activity with p-nitrophenyl phosphate
-
-
?
alpha-glycerophosphate + H2O
glycerol + phosphate
-
low activity with alpha-isomer
-
?
alpha-naphthyl phosphate + H2O
1-naphthol + phosphate
-
hydrolyzed by isoenzyme II and III, no activity with isoenzyme I
-
-
?
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
-
-
-
?
alpha-naphthyl phosphate + H2O
alpha-naphthol + phosphate
-
-
-
?
AMP + H2O
adenosine + phosphate
-
wild-type activity is 9.6% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 18.2fold higher than activity from wild-type enzyme
-
-
?
ATP + H2O
?
-
57.8% of the activity with 4-nitrophenyl phosphate
-
-
?
ATP + H2O
?
-
wild-type activity is 15.4% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 6.9fold higher than activity from wild-type enzyme
-
-
?
beta-glycerophosphate + H2O
glycerol + phosphate
-
20% of activity with p-nitrophenyl phosphate
-
-
?
beta-glycerophosphate + H2O
glycerol + phosphate
-
20% of activity with p-nitrophenyl phosphate
-
-
?
beta-glycerophosphate + H2O
glycerol + phosphate
uranium precipitation assay: uranyl carbonate solution in carbonate-bicarbonate buffer, supplemented with beta-glycerophosphate as the substrate at 30°C under static nongrowing conditions in a final volume of 5 ml
-
-
?
beta-glyceryl phosphate + H2O
glycerol + phosphate
-
-
-
?
bis(p-nitrophenyl) phosphate + H2O
?
6.1% of the activity with 4-nitrophenyl phosphate
-
-
?
bis(p-nitrophenyl) phosphate + H2O
?
6.1% of the activity with 4-nitrophenyl phosphate
-
-
?
casein + H2O
?
-
enzyme shows protein phosphatase activity
-
?
casein + H2O
?
-
enzyme shows protein phosphatase activity
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
-
36% of the activity with 4-nitrophenyl phosphate
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
-
5.2% of the activity with 4-nitrophenyl phosphate
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
-
wild-type activity is 2.2% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 52fold higher than activity from wild-type enzyme
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
-
32% of the activity with 4-nitrophenyl phosphate
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
-
wild-type activity is 9.6% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 21fold higher than activity from wild-type enzyme
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
-
60% of the activity with 4-nitrophenyl phosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
-
19.4% of the activity with 4-nitrophenyl phosphate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
-
wild-type activity is 6.6% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 34.5fold higher than activity from wild-type enzyme
-
-
?
glucose 1-phosphate + H2O
glucose + phosphate
-
93% of the activity with 4-nitrophenyl phosphate
-
-
?
Glucose 6-phosphate + H2O
Glucose + phosphate
-
alkaline phosphatase F I
-
-
?
Glucose 6-phosphate + H2O
Glucose + phosphate
-
alkaline phosphatase F II
-
-
?
glycerophosphate + H2O
?
248.7% of the activity with 4-nitrophenyl phosphate
-
-
?
glycerophosphate + H2O
?
248.7% of the activity with 4-nitrophenyl phosphate
-
-
?
glycerophosphate + H2O
glycerol + phosphate
-
63% of the activity with 4-nitrophenyl phosphate
-
-
?
L-histidinyl phosphate + H2O
L-histidinol + phosphate
-
-
-
?
O-phosphoserine + H2O
serine + phosphate
-
wild-type activity is 14.0% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 10.9fold higher than activity from wild-type enzyme
-
-
?
O-phosphothreonine + H2O
threonine + phosphate
-
wild-type activity is 21.3% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 9.8fold higher than activity from wild-type enzyme
-
-
?
O-phosphotyrosine + H2O
tyrosine + phosphate
-
wild-type activity is 68.3% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 8.6fold higher than activity from wild-type enzyme
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
hydrolyzed by isoenzyme I, II or III
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
activities towards tyrosine phosphatase substrate ENCpYINASL and calcineurin substrate DLDVPIPGRDFRRVpSVAAE were also detected, further substrates: ATP, sn-glycerol 3-phosphate
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
Synechococcus elongatus PCC 7942 = FACHB-805 R2
-
-
-
-
?
phosphoethanolamine + H2O
phosphate + ethanolamine
-
hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I
-
-
?
phosphorylated acetyl-CoA carboxylase + H2O
acetyl-CoA carboxylase + phosphate
-
-
-
-
?
phosphorylated acetyl-CoA carboxylase + H2O
acetyl-CoA carboxylase + phosphate
-
-
-
-
?
phosphorylcholine + H2O
phosphate + choline
-
hydrolyzed by isoenzyme III, no activity with isoenzyme I and II
-
-
?
phosphoserine + H2O
phosphate + Ser
-
9.3% of the activity with 4-nitrophenyl phosphate
-
-
?
phosphoserine + H2O
phosphate + Ser
-
hydrolyzed by isoenzyme II, weak activity with isoenzyme I and II
-
-
?
additional information
?
-
-
the purified alkaline phosphatase removes the 5'-phosphate group of a linearized plasmid without showing DNAase activity
-
-
?
additional information
?
-
-
motion required for the formation of the enzyme-phosphate complex is minimal on the part of alkaline phosphatase
-
-
?
additional information
?
-
-
TNAP is an essential component of serum calcification activity and that its role in the shotgun mechanism is to activate the serum nucleator of apatite crystal formation
-
-
?
additional information
?
-
-
enzmye exclusively phosphomonoesters as a substrate, and not phosphodiesters
-
-
?
additional information
?
-
-
alkaline phosphatase activity is decreased during vitamin C deficiency. The decreased expression of collagen, alkaline phosphatase, and osteocalcin can explain the defects in bone caused by scurvy
-
-
?
additional information
?
-
-
involvement of the enzyme in sugar metabolism
-
-
?
additional information
?
-
-
could be a component of muscle regulatory mechanism at the biochemical level secondary to hyper-regulation of Chasmagnathus granulatus
-
-
?
additional information
?
-
-
the enzyme is involved in the biochemical amplification process of excitation or adaption
-
-
?
additional information
?
-
-
the enzyme also catalyses the transfer of the phosphoryl group to alcohols
-
?
additional information
?
-
-
the enzyme is involved in recovering of phosphate esters when free phosphate is depleted
-
?