BRENDA Home
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
endonucleolytic cleavage of poly(A) to fragments terminated by 3'-hydroxy and 5'-phosphate groups
Synonyms
endoribonuclease iv,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
endoribonuclease IV
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
endonucleolytic cleavage of poly(A) to fragments terminated by 3'-hydroxy and 5'-phosphate groups
endonucleolytic cleavage of poly(A) to fragments terminated by 3'-hydroxy and 5'-phosphate groups
random cleavage
-
endonucleolytic cleavage of poly(A) to fragments terminated by 3'-hydroxy and 5'-phosphate groups
forms oligonucleotides with an average chain length of 10
-
endonucleolytic cleavage of poly(A) to fragments terminated by 3'-hydroxy and 5'-phosphate groups
forms oligonucleotides with an average chain length of 10
-
endonucleolytic cleavage of poly(A) to fragments terminated by 3'-hydroxy and 5'-phosphate groups
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of phosphoric ester
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MS2 RNA + H2O
?
-
-
-
-
?
poly(A) + H2O
oligonucleotides
R17 RNA + H2O
?
-
-
-
-
?
RNA + H2O
oligonucleotides
additional information
?
-
-
not: poly(C), poly(U), poly(G), double-stranded poly(A)*poly(U), DNA
-
-
?
poly(A) + H2O
oligonucleotides
-
-
with an average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
poly(A) + H2O
oligonucleotides
-
-
with an average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
poly(A) + H2O
oligonucleotides
-
-
with an average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
-
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
-
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
-
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
-
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
cleavage of the poly(A)-tail of mRNA
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
processing of nuclear RNA to mRNA
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
RNA + H2O
oligonucleotides
RNA + H2O
oligonucleotides
-
-
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
-
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
-
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
-
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
cleavage of the poly(A)-tail of mRNA
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
RNA + H2O
oligonucleotides
-
processing of nuclear RNA to mRNA
average chain length of 10, terminated by 3'-hydroxyl and 5'-phosphate groups
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mn2+
-
optimum: 0.5 mM, more effective than Mg2+
additional information
-
does not require divalent cations
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EDTA
-
presence of 2 mM EDTA results in total inhibition, under 0.2 mM: no effect
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Infections
Dramatic increase in poly(A) synthesis after infection of Molt-3 cells with HIV.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Quail
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
45000
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, in solution 5, addition of 1 mg/ml albumin, after 6 months 30% of activity were lost
-
-20°C, in water, retains full activity for 7 years
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
15000fold, nearly to homogeneity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Muller, W.E.G.
Endoribonuclease IV. A poly(A)-specific ribonuclease from chick oviduct. 1. Purification of the enzyme
Eur. J. Biochem.
70
241-248
1976
Gallus gallus
brenda
Muller, W.E.G.; Seibert, G.; Steffen, R.; Zahn, R.K.
Endoribonuclease IV. 2. Further investigation on the specificity
Eur. J. Biochem.
70
249-258
1976
Gallus gallus, Coturnix japonica
brenda
Bellemare, G.; Jordan, B.R.; Monier, R.
Demonstration of a highly exposed region in Escherichia coli 5 s RNA by partial hydrolysis with ribonuclease IV and sheep kidney nuclease
J. Mol. Biol.
71
307-315
1972
Escherichia coli
brenda
Jacobson, A.B.; Spahr, P.F.
Studies on the secondary structure of single-stranded RNA from the bacteriophage MS2. II Analysis of the RNase IV cleavage products
J. Mol. Biol.
115
279-294
1977
Escherichia coli
brenda
Spahr, P.F.; Gesteland, R.F.
Specific cleavage of bacteriophage R17 RNA by an endonuclease isolated from E. coli MRE-600
Proc. Natl. Acad. Sci. USA
59
876-883
1968
Escherichia coli
brenda
Select items on the left to see more content.
html completed