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EC Tree
The expected taxonomic range for this enzyme is: Synechococcus elongatus PCC 7942 = FACHB-805
Reaction Schemes
Endonucleolytic cleavage to a 5'-phosphomonoester
Synonyms
2'-O-methyl RNase,
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Endonucleolytic cleavage to a 5'-phosphomonoester
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hydrolysis of phosphoric ester
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RNA, methylated in the 2'-hydroxy position of ribose + H2O
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additional information
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no hydrolysis of: deoxyribonucleic acid, normal yeast RNA
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RNA, methylated in the 2'-hydroxy position of ribose + H2O
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chemically 2'-O-methylated yeast RNA
the products are terminated in 5'-phosphates
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RNA, methylated in the 2'-hydroxy position of ribose + H2O
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naturally occuring 2'-O-methyl RNA isolated from rhapidosomes
the products are terminated in 5'-phosphates
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additional information
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1 mM Hg2+ has no effect
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additional information
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4 - 7.5
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pH 4.0: considerable activity at, pH 7.5: optimum
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brenda
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5 min, retains almost two-thirds of its initial activity
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5 min, complete inactivation
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Norton, J.; Roth, J.S.
A ribonuclease specific for 2'-methylated ribonucleic acid
J. Biol. Chem.
242
2029-2034
1967
Synechococcus elongatus PCC 7942 = FACHB-805
brenda
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