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EC Tree
The enzyme appears in viruses and cellular organisms
Reaction Schemes
exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
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bacteriophage SP3 deoxyribonuclease
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deoxyribonucleate 5-dinucleotidase
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deoxyribonucleic 5-dinucleotidohydrolase
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DNA 5-dinucleotidohydrolase
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EC 3.1.4.31
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formerly
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exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
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hydrolysis of phosphoric ester
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oligo- and polydeoxyribonucleotides + H2O
dinucleotide + oligonucleotide
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hydrolysis on polynucleotides from the 5-termini of single-stranded DNA
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single-stranded DNA + H2O
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single-stranded DNA + H2O
dinucleotide + trinucleotide
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hydrolysis from the 5-and 3-termini of single-stranded DNA
general structure of dinucleotide is d-pX-pY and of trinucleotide is d-pX-pY-pZ
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sorbitol-derivatized octanucleotides + H2O
dinucleotide + oligonucleotide
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single-stranded DNA + H2O
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Ca2+
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slight activity, no activity in less than 6.7 mM
Mg2+
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optimum for activation between 13 and 25 mM
Mn2+
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at 1.3 mM 44% of activation by Mg2+
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N-cyclohexyl-N'-beta-(4-methylmorpholinium)-ethylcarbodiimide-derivatized DNA
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blocks enzyme activity absolutely, inhibition independent of nucleotide sequences
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brenda
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infected Bacillus subtilis
brenda
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I7GZP8_9HELI
263
0
29767
TrEMBL
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A0A2X3BFB2_9HELI
265
0
30325
TrEMBL
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A0A448TR47_9HELI
269
0
30545
TrEMBL
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A0A377JVW7_9HELI
268
0
30416
TrEMBL
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A0A377Q111_9HELI
269
0
30558
TrEMBL
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A0A377JVA4_9HELI
268
0
30411
TrEMBL
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DEAE-cellulose, phosphocellulose, sucrose gradient sedimentation
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analysis
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determination of the direction of hydrolysis of processive exonucleases
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Ho, N.W.Y.
Methods for limiting the action of SP3 DNAase and for the determination of the direction of hydrolysis of processive exonucleases
Biochim. Biophys. Acta
563
393-399
1979
Enterobacteria phage SP
brenda
Trilling, D.M.; Aposhian, H.V.
Sequential cleavage of dinucleotides from DNA by phage SP3 DNase
Proc. Natl. Acad. Sci. USA
60
214-221
1968
Enterobacteria phage SP
brenda
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