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IUBMB Comments The enzyme participates in the methylaspartate cycle, an anaplerotic pathway that operates in some members of the haloarchaea and forms malate from acetyl-CoA.
Synonyms MCT , mesaconate:succinyl-CoA CoA transferase,
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mesaconate:succinyl-CoA CoA transferase
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mesaconate + succinyl-CoA = 2-methylfumaryl-CoA + succinate
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MetaCyc
methylaspartate cycle
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succinyl-CoA:mesaconate CoA transferase
The enzyme participates in the methylaspartate cycle, an anaplerotic pathway that operates in some members of the haloarchaea and forms malate from acetyl-CoA.
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acrylate + succinyl-CoA
? + succinate
Substrates: low activity
?
glutarate + succinyl-CoA
? + succinate
Substrates: low activity
?
itaconate + succinyl-CoA
? + succinate
Substrates: low activity
?
mesaconate + succinyl-CoA
2-methylfumaryl-CoA + succinate
methylsuccinate + succinyl-CoA
? + succinate
Substrates: high activity, not a natural substrate
?
mesaconate + succinyl-CoA
2-methylfumaryl-CoA + succinate
Substrates: the enzyme is involved in the methylaspartate cycle
?
mesaconate + succinyl-CoA
2-methylfumaryl-CoA + succinate
Substrates: whereas the enzyme is highly specific for succinyl-CoA as a CoA-donor, it can activate other carboxylic acids, i.e., methylsuccinate, itaconate, acrylate, and glutarate
?
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mesaconate + succinyl-CoA
2-methylfumaryl-CoA + succinate
Substrates: the enzyme is involved in the methylaspartate cycle
?
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KCl
the enzyme is dependent on the presence of KCl and shows highest activity at 4 M KCl
additional information
no requirement for divalent cations
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CaCl2
5 mM, 5% inhibition
CoCl2
5 mM, 10% inhibition
MgCl2
5 mM, 7% inhibition
MnCl2
5 mM, 24% inhibition
NiCl2
5 mM, 5% inhibition
additional information
no inhibition by EDTA
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39.3
Acrylate
37°C, pH 7.8
40.9
Glutarate
37°C, pH 7.8
0.6 - 2.9
Methylsuccinate
2.8
succinyl-CoA
37°C, pH 7.8
1.3
mesaconate
37°C, pH 7.8, 1 mM succinyl-CoA
7.1
mesaconate
37°C, pH 7.8, 5 mM succinyl-CoA
0.6
Methylsuccinate
37°C, pH 7.8, 1 mM succinyl-CoA
2.9
Methylsuccinate
37°C, pH 7.8, 5 mM succinyl-CoA
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12.2
mesaconate
37°C, pH 7.8
12.2
succinyl-CoA
37°C, pH 7.8
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0.12
Acrylate
37°C, pH 7.8
0.32
Glutarate
37°C, pH 7.8
9.1 - 12.4
Methylsuccinate
17.8
succinyl-CoA
37°C, pH 7.8
4.6
mesaconate
37°C, pH 7.8, 5 mM succinyl-CoA
9.8
mesaconate
37°C, pH 7.8, 1 mM succinyl-CoA
9.1
Methylsuccinate
37°C, pH 7.8, 5 mM succinyl-CoA
12.4
Methylsuccinate
37°C, pH 7.8, 1 mM succinyl-CoA
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4.3
calculated from sequence
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UniProt
brenda
Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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evolution
the mct gene is mainly transmitted vertically among haloarchaea
metabolism
the enzyme catalyzes a reaction in the methylaspartate cycle
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MCT_HALHT
Haloarcula hispanica (strain ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM B-1755)
396
0
42996
Swiss-Prot
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MCT_HALMA
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
396
0
42956
Swiss-Prot
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homodimer
2 * 48000, SDS-PAGE
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Haloferax volcanii strain H1209 is used for the heterologous expression of the Haloarcula hispanica proteins
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Borjian, F.; Johnsen, U.; Schoenheit, P.; Berg, I.A.
Succinyl-CoA mesaconate CoA-transferase and mesaconyl-CoA hydratase, enzymes of the methylaspartate cycle in Haloarcula hispanica
Front. Microbiol.
8
1683
2017
Haloarcula hispanica (G0HQ31)
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