Reference on EC 2.8.1.6 - biotin synthase
Please use the Reference Search for a specific query.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Baldet, P.; Gerbling, H.; Axiotis, S.; Douce, R.
Biotin biosynthesis in higher plant cells. Identification of intermediates
Eur. J. Biochem.
217
479-485
1993
Lavandula vera
Izumi, Y.; Kano, Y.; Inagaki, K.; Kawase, N.; Tani, Y.; Yamada, H.
Characterization of biotin biosynthetic enzymes of Bacillus sphaericus: a dethiobiotin producing bacterium
Agric. Biol. Chem.
45
1983-1989
1981
Lysinibacillus sphaericus
-
Shiuan, D.; Campbell, A.
Transcriptional regulation and gene arrangement of Escherichia coli, Citrobacter freundii and Salmonella typhimurium biotin operons
Gene
67
203-211
1988
Citrobacter freundii, Escherichia coli
Zhang, S.; Sanyal, I.; Bulboaca, G.H.; Rich, A.; Flint, D.H.
The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase
Arch. Biochem. Biophys.
309
29-35
1994
Bacillus subtilis, Saccharomyces cerevisiae
Bui, T.S.; Marquet, A.
Biotin synthase of Bacillus sphaericus
Methods Enzymol.
279
356-362
1997
Lysinibacillus sphaericus
Flint, D.H.; Allen, R.M.
Purification and characterization of biotin synthases
Methods Enzymol.
279
349-356
1997
Lysinibacillus sphaericus, Escherichia coli
Birch, O.M.; Fuhrmann, M.; Shaw, N.M.
Biotin synthase from Escherichia coli, an investigation of the low molecular weight and protein components required for activity in vitro
J. Biol. Chem.
270
19158-19165
1995
Escherichia coli
Sanyal, I.; Cohen, G.; Flint, D.H.
Biotin synthase: purification, characterization as a [2Fe-2S]cluster protein, and in vitro activity of the Escherichia coli bioB gene product
Biochemistry
33
3625-3631
1994
Escherichia coli
Bui, B.T.S.; Florentin, D.; Fournier, F.; Ploux, O.; Mejean, A.; Marquet, A.
Biotin synthase mechanism: on the origin of sulfur
FEBS Lett.
440
226-230
1998
Lysinibacillus sphaericus, Escherichia coli
Mejean, A.; Bui, B.T.S.; Florentin, D.; Ploux, O.; Izumi, Y.; Marquet, A.
Highly purified biotin synthase can transform dethiobiotin into biotin in the absence of any other protein, in the presence of photoreduced deazaflavin
Biochem. Biophys. Res. Commun.
217
1231-1237
1995
Lysinibacillus sphaericus
Ohshiro, T.; Yamamoto, M.; Izumi, Y.; Bui, B.T.S.; Florentin, D.; Marquet, A.
Enzymatic conversion of dethiobiotin in cell-free extracts of a Bacillus sphaericus bioB transformant
Biosci. Biotechnol. Biochem.
58
1738-1741
1994
Lysinibacillus sphaericus
Weaver, L.M.; Yu, F.; Wurtele, E.S.; Nikolau, B.J.
Characterization of the cDNA and gene coding for the biotin synthase of Arabidopsis thaliana
Plant Physiol.
110
1021-1028
1996
Arabidopsis thaliana
Patton, D.A.; Jonson, M.; Ward, E.R.
Biotin synthase from Arabidopsis thaliana. cDNA isolation and characterization of gene expression
Plant Physiol.
112
371-378
1996
Arabidopsis thaliana
Sanyal, I.; Gibson, K.J.; Flint, D.H.
Escherichia coli biotin synthase: an investigation into the factors required for its activity and its sulfur donor
Arch. Biochem. Biophys.
326
48-56
1996
Escherichia coli
Shaw, N.M.; Birch, O.M.; Tinschert, A.; Venetz, V.; Dietrich, R.; Savoy, L.A.
Biotin synthase from Escherichia coli: isolation of an enzyme-generated intermediate and stoichiometry of S-adenosylmethionine use
Biochem. J.
330
1079-1085
1998
Escherichia coli
Ugulava, N.B.; Sacanell, C.J.; Jarrett, J.T.
Spectroscopic changes during a single turnover of biotin synthase: Destruction of a [2Fe-2S] cluster accompanies sulfur insertion
Biochemistry
40
8352-8358
2001
Escherichia coli
Berkovitch, F.; Nicolet, Y.; Wan, J.T.; Jarrett, J.T.; Drennan, C.L.
Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme
Science
303
76-79
2004
Escherichia coli (P12996), Escherichia coli
Cosper, M.M.; Cosper, N.J.; Hong, W.; Shokes, J.E.; Broderick, W.E.; Broderick, J.B.; Johnson, M.K.; Scott, R.A.
Structural studies of the interaction of S-adenosylmethionine with the [4Fe-4S] clusters in biotin synthase and pyruvate formate-lyase activating enzyme
Protein Sci.
12
1573-1577
2003
Escherichia coli
Ugulava, N.B.; Surerus, K.K.; Jarrett, J.T.
Evidence from Mossbauer spectroscopy for distinct [2Fe-2S]2+ and [4Fe-4S]2+ cluster binding sites in biotin synthase from Escherichia coli
J. Am. Chem. Soc.
124
9050-9051
2002
Escherichia coli
Birch, O.M.; Hewitson, K.S.; Fuhrmann, M.; Burgdorf, K.; Baldwin, J.E.; Roach, P.L.; Shaw, N.M.
MioC is an FMN-binding protein that is essential for Escherichia coli biotin synthase activity in vitro
J. Biol. Chem.
275
32277-32280
2000
Escherichia coli
Bui, B.T.S.; Benda, R.; Schuenemann, V.; Florentin, D.; Trautwein, A.X.; Marquet, A.
Fate of the (2Fe-2S)2+ cluster of Escherichia coli biotin synthase during reaction: A Moessbauer characterization
Biochemistry
42
8791-8798
2003
Escherichia coli
Cosper, M.M.; Jameson, G.N.; Eidsness, M.K.; Huynh, B.H.; Johnson, M.K.
Recombinant Escherichia coli biotin synthase is a [2Fe-2S](2+) protein in whole cells
FEBS Lett.
529
332-336
2002
Escherichia coli
Cosper, M.M.; Jameson, G.N.L.; Davydov, R.; Eidsness, M.K.; Hoffman, B.M.; Huynh, B.H.; Johnson, M.K.
The [4Fe-4S]2+ cluster in reconstituted biotin synthase binds S-adenosyl-L-methionine
J. Am. Chem. Soc.
124
14006-14007
2002
Escherichia coli
Farh, L.; Hwang, S.Y.; Steinrauf, L.; Chiang, H.J.; Shiuan, D.
Structure-function studies of Escherichia coli biotin synthase via a chemical modification and site-directed mutagenesis approach
J. Biochem.
130
627-635
2001
Escherichia coli
Hewitson, K.S.; Baldwin, J.E.; Shaw, N.M.; Roach, P.L.
Mutagenesis of the proposed iron-sulfur cluster binding ligands in Escherichia coli biotin synthase
FEBS Lett.
466
372-376
2000
Escherichia coli
Kiyasu, T.; Asakura, A.; Nagahashi, Y.; Hoshino, T.
Biotin synthase of Bacillus subtilis shows less reactivity than that of Escherichia coli in in vitro reaction systems
Arch. Microbiol.
179
26-32
2002
Bacillus subtilis, Escherichia coli
McIver, L.; Baxter, R.L.; Campopiano, D.J.
Identification of the [Fe-S] cluster-binding residues of Escherichia coli biotin synthase
J. Biol. Chem.
275
13888-13894
2000
Escherichia coli
Ollagnier-de Choudens, S.; Sanakis, Y.; Hewitson, K.S.; Roach, P.; Munck, E.; Fontecave, M.
Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli
J. Biol. Chem.
277
13449-13454
2002
Escherichia coli
Ollagnier-de-Choudens, S.; Mulliez, E.; Fontecave, M.
The PLP-dependent biotin synthase from Escherichia coli: mechanistic studies
FEBS Lett.
532
465-468
2002
Escherichia coli
Ollagnier-de-Choudens, S.; Mulliez, E.; Hewitson, K.S.; Fontecave, M.
Biotin synthase is a pyridoxal phosphate-dependent cysteine desulfurase
Biochemistry
41
9145-9152
2002
Escherichia coli
Picciocchi, A.; Douce, R.; Alban, C.
Biochemical characterization of the Arabidopsis biotin synthase reaction. The importance of mitochondria in biotin synthesis
Plant Physiol.
127
1224-1233
2001
Arabidopsis thaliana
Picciocchi, A.; Douce, R.; Alban, C.
The plant biotin synthase reaction. Identification and characterization of essential mitochondrial accessory protein components
J. Biol. Chem.
278
24966-24975
2003
Arabidopsis thaliana (O49543), Arabidopsis thaliana (Q8S904), Arabidopsis thaliana (Q8W3L1), Arabidopsis thaliana (Q9M0V0), Arabidopsis thaliana
Ugulava, N.B.; Frederick, K.K.; Jarrett, J.T.
Control of adenosylmethionine-dependent radical generation in biotin synthase: A kinetic and thermodynamic analysis of substrate binding to active and inactive forms of BioB
Biochemistry
42
2708-2719
2003
Escherichia coli
Ugulava, N.B.; Gibney, B.R.; Jarrett, J.T.
Biotin synthase contains two distinct iron-sulfur cluster binding sites: Chemical and spectroelectrochemical analysis of iron-sulfur cluster interconversions
Biochemistry
40
8343-8351
2001
Escherichia coli
Kiyasu, T.; Asakura, A.; Nagahashi, Y.; Hoshino, T.
Contribution of cysteine desulfurase (NifS protein) to the biotin synthase reaction of Escherichia coli
J. Bacteriol.
182
2879-2885
2000
Escherichia coli
Mihara, H.; Esaki, N.
Bacterial cysteine desulfurases: their function and mechanisms
Appl. Microbiol. Biotechnol.
60
12-23
2002
Escherichia coli
Gibson, K.J.; Pelletier, D.A.; Turner, I.M., Sr.
Transfer of sulfur to biotin from biotin synthase (BioB protein)
Biochem. Biophys. Res. Commun.
254
632-635
1999
Escherichia coli
Tse Sum Bui, B.; Lotierzo, M.; Escalettes, F.; Florentin, D.; Marquet, A.
Further investigation on the turnover of Escherichia coli biotin synthase with dethiobiotin and 9-mercaptodethiobiotin as substrates
Biochemistry
43
16432-16441
2004
Escherichia coli
Mader Cosper, M.; Jameson, G.N.L.; Hernandez, H.L.; Krebs, C.; Huynh, B.H.; Johnson, M.K.
Characterization of the cofactor composition of Escherichia coli biotin synthase
Biochemistry
43
2007-2021
2004
Escherichia coli
Jameson, G.N.; Cosper, M.M.; Hernandez, H.L.; Johnson, M.K.; Huynh, B.H.
Role of the [2Fe-2S] cluster in recombinant Escherichia coli biotin synthase
Biochemistry
43
2022-2031
2004
Escherichia coli
Choi-Rhee, E.; Cronan, J.E.
Biotin synthase is catalytic in vivo, but catalysis engenders destruction of the protein
Chem. Biol.
12
461-468
2005
Escherichia coli
Tse Sum Bui, B.; Florentin, D.; Marquet, A.; Benda, R.; Trautwein, A.X.
Mossbauer studies of Escherichia coli biotin synthase: evidence for reversible interconversion between [2Fe-2S]2+ and [4Fe-4S]2+ clusters
FEBS Lett.
459
411-414
1999
Escherichia coli
Hong, Y.; Chen, Y.; Farh, L.; Yang, W.; Liao, C.; Shiuan, D.
Recombinant Candida utilis for the production of biotin
Appl. Microbiol. Biotechnol.
71
211-221
2006
Cyberlindnera jadinii (Q9HF48), Cyberlindnera jadinii
Lotierzo, M.; Raux, E.; Tse Sum Bui, B.; Goasdoue, N.; Libot, F.; Florentin, D.; Warren, M.J.; Marquet, A.
Biotin synthase mechanism: mutagenesis of the YNHNLD conserved motif
Biochemistry
45
12274-12281
2006
Escherichia coli
Broach, R.B.; Jarrett, J.T.
Role of the [2Fe-2S]2+ cluster in biotin synthase: mutagenesis of the atypical metal ligand arginine 260
Biochemistry
45
14166-14174
2006
Escherichia coli
Tse Sum Bui, B.; Mattioli, T.A.; Florentin, D.; Bolbach, G.; Marquet, A.
Escherichia coli biotin synthase produces selenobiotin. Further evidence of the involvement of the [2Fe-2S]2+ cluster in the sulfur insertion step
Biochemistry
45
3824-3834
2006
Escherichia coli
Muehlenhoff, U.; Gerl, M.J.; Flauger, B.; Pirner, H.M.; Balser, S.; Richhardt, N.; Lill, R.; Stolz, J.
The iron-sulfur cluster proteins Isa1 and Isa2 are required for the function but not for the de novo synthesis of the Fe/S clusters of biotin synthase in Saccharomyces cerevisiae
Eukaryot. Cell
6
495-504
2007
Saccharomyces cerevisiae, Saccharomyces cerevisiae W303-1A
Reyda, M.R.; Dippold, R.; Dotson, M.E.; Jarrett, J.T.
Loss of iron-sulfur clusters from biotin synthase as a result of catalysis promotes unfolding and degradation
Arch. Biochem. Biophys.
471
32-41
2008
Escherichia coli
Taylor, A.M.; Farrar, C.E.; Jarrett, J.T.
9-Mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase
Biochemistry
47
9309-9317
2008
Escherichia coli
Abdel-Hamid, A.M.; Cronan, J.E.
In vivo resolution of conflicting in vitro results: Synthesis of biotin from dethiobiotin does not require pyridoxal phosphate
Chem. Biol.
14
1215-1220
2007
Escherichia coli
Lotierzo, M.; Bui, B.T.; Leech, H.K.; Warren, M.J.; Marquet, A.; Rigby, S.E.
Iron-sulfur cluster dynamics in biotin synthase: a new [2Fe-2S](1+) cluster
Biochem. Biophys. Res. Commun.
381
487-490
2009
Escherichia coli
Reyda, M.R.; Fugate, C.J.; Jarrett, J.T.
A complex between biotin synthase and the iron-sulfur cluster assembly chaperone HscA that enhances in vivo cluster assembly
Biochemistry
48
10782-10792
2009
Escherichia coli
Farrar, C.; Jarrett, J.
Protein residues that control the reaction trajectory in S-adenosylmethionine radical enzymes: Mutagenesis of asparagine 153 and aspartate 155 in Escherichia coli biotin synthase
Biochemistry
48
2448-2458
2009
Escherichia coli
Fuchs, M.G.; Meyer, F.; Ryde, U.
A combined computational and experimental investigation of the [2Fe-2S] cluster in biotin synthase
J. Biol. Inorg. Chem.
15
203-212
2010
Escherichia coli (P12996)
Delli-Bovi, T.; Spalding, M.; Prigge, S.
Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli
BMC Biotechnol.
10
73
2010
Escherichia coli
Farrar, C.E.; Siu, K.K.; Howell, P.L.; Jarrett, J.T.
Biotin synthase exhibits burst kinetics and multiple turnovers in the absence of inhibition by products and product-related biomolecules
Biochemistry
49
9985-9996
2010
Escherichia coli
Fugate, C.J.; Jarrett, J.T.
Biotin synthase: insights into radical-mediated carbon-sulfur bond formation
Biochim. Biophys. Acta
1824
1213-1222
2012
Escherichia coli (P12996)
Fugate, C.J.; Stich, T.A.; Kim, E.G.; Myers, W.K.; Britt, R.D.; Jarrett, J.T.
9-Mercaptodethiobiotin is generated as a ligand to the [2Fe-2S]+ cluster during the reaction catalyzed by biotin synthase from Escherichia coli
J. Am. Chem. Soc.
134
9042-9045
2012
Escherichia coli
Kamachi, T.; Kouno, T.; Doitomi, K.; Yoshizawa, K.
Generation of adenosyl radical from S-adenosylmethionine (SAM) in biotin synthase
J. Inorg. Biochem.
105
850-857
2011
Escherichia coli (P12996)
Kim, H.; Kim, J.; Park, Y.
Transcriptome and gene ontology (GO) enrichment analysis reveals genes involved in biotin metabolism that affect L-Lysine production in Corynebacterium glutamicum
Int. J. Mol. Sci.
17
353
2016
Corynebacterium glutamicum (P46396), Corynebacterium glutamicum, Corynebacterium glutamicum DSM 20300 (P46396)
Kamiyama, S.; Ohnuki, R.; Moriki, A.; Abe, M.; Ishiguro, M.; Sone, H.
The effects of light and temperature on biotin synthesis in pea sprouts
J. Nutr. Sci. Vitaminol.
62
19-25
2016
Pisum sativum
Bockman, M.R.; Engelhart, C.A.; Cramer, J.D.; Howe, M.D.; Mishra, N.K.; Zimmerman, M.; Larson, P.; Alvarez-Cabrera, N.; Park, S.W.; Boshoff, H.I.M.; Bean, J.M.; Young, V.G.; Ferguson, D.M.; Dartois, V.; Jarrett, J.T.; Schnappinger, D.; Aldrich, C.C.
Investigation of ( S)-(-)-acidomycin a selective antimycobacterial natural product that inhibits biotin synthase
ACS Infect. Dis.
5
598-617
2019
Mycobacterium tuberculosis (P9WPQ7), Mycobacterium tuberculosis H37Rv (P9WPQ7)
Tao, L.; Stich, T.A.; Fugate, C.J.; Jarrett, J.T.; Britt, R.D.
EPR-derived structure of a paramagnetic intermediate generated by biotin synthase BioB
J. Am. Chem. Soc.
140
12947-12963
2018
Escherichia coli (P12996)
Kwon, K.; Bekal, S.; Domier, L.; Lambert, K.
Active and inactive forms of biotin synthase occur in Heterodera glycines
J. Nematol.
51
e2019-69
2019
Heterodera glycines (F1LLQ0)
-
Bali, A.P.; Lennox-Hvenekilde, D.; Myling-Petersen, N.; Buerger, J.; Salomonsen, B.; Gronenberg, L.S.; Sommer, M.O.A.; Genee, H.J.
Improved biotin, thiamine, and lipoic acid biosynthesis by engineering the global regulator IscR
Metab. Eng.
60
97-109
2020
Escherichia coli, Escherichia coli BW25113
Magwamba, C.C.; Rukseree, K.; Palittapongarnpim, P.
Cloning, expression and characterization of histidine-tagged biotin synthase of Mycobacterium tuberculosis
Tuberculosis
98
42-49
2016
Mycobacterium tuberculosis (P9WPQ7), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WPQ7)
html completed
