Information on EC 2.8.1.1 - thiosulfate sulfurtransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
2.8.1.1
-
RECOMMENDED NAME
GeneOntology No.
thiosulfate sulfurtransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thiosulfate + cyanide = sulfite + thiocyanate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfur atom transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
thiosulfate disproportionation IV (rhodanese)
-
-
non-pathway related
-
-
Sulfur metabolism
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
thiosulfate:cyanide sulfurtransferase
A few other sulfur compounds can act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-04-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
cabbage
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
vervet monkey
-
-
Manually annotated by BRENDA team
Columbia domesticus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
comparison of enzyme activity with several tissues of sheep, cattle, goat, camel, dog, chicken and human
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain H37Rv
-
-
Manually annotated by BRENDA team
strain H37Rv
-
-
Manually annotated by BRENDA team
chinese hamster
-
-
Manually annotated by BRENDA team
parsley
-
-
Manually annotated by BRENDA team
serovar typhimurium
-
-
Manually annotated by BRENDA team
spinach
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strains: O90, O77, O10, O82, O80
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-(dimethylamino)-4'-azobenzene sulfinate + thiosulfate
4-(dimethylamino)-4'-azobenzene thiosulfonate + SO32-
show the reaction diagram
-
-
-
-
?
4-(dimethylamino)-4'-azobenzene thiosulfonate + cyanide
4-(dimethylamino)-4'-azobenzene sulfinate + SCN-
show the reaction diagram
-
-
-
-
?
4-(dimethylamino)-4'-azobenzene thiosulfonate + glutathione
4-(dimethylamino)-4'-azobenzene sulfinate + reduced glutathione
show the reaction diagram
-
-
-
-
?
5-dimethylamino-1-naphthalene sulfinate + thiosulfate
5-dimethylamino-1-naphthalene thiosulfonate + SO32-
show the reaction diagram
-
-
-
-
?
5-dimethylamino-1-naphthalene thiosulfonate + cyanide
5-dimethylamino-1-naphthalene sulfinate + SCN-
show the reaction diagram
-
-
-
-
?
5-dimethylamino-1-naphthalene thiosulfonate + glutathione
5-dimethylamino-1-naphthalene sulfinate + reduced glutathione
show the reaction diagram
-
-
-
-
?
alkyl sulfinate + cyanide
?
show the reaction diagram
aryl sulfinate + cyanide
?
show the reaction diagram
cyanide + 2-aminoethanethiosulfate
?
show the reaction diagram
-
K249A
-
-
?
cyanide + 3-mercaptopyruvate
?
show the reaction diagram
-
-
-
?
cyanide + ethanethiosulfate
?
show the reaction diagram
-
K249A
-
-
?
cyanide + p-toluenethiosulfate
?
show the reaction diagram
-
K249A
-
-
?
cysteine + cyanide
cyanoalanine + H2S
show the reaction diagram
H2S + cyanide
?
show the reaction diagram
-
very poor substrate
-
-
?
H2S + cyanide
thiocyanate
show the reaction diagram
persulfide + cyanide
?
show the reaction diagram
-
-
-
-
?
polysulfide + cyanide
? + thiocyanate
show the reaction diagram
-
-
-
-
?
reduced thioredoxin + methane thiosulfonate
?
show the reaction diagram
-
only the less negative rhodanese isoform catalyzes the oxidation of thioredoxin
-
-
r
tetrathionate + cyanide
? + thiocyanate
show the reaction diagram
-
-
-
-
?
thiocystine + cyanide
?
show the reaction diagram
thioredoxin + thiosulfate
?
show the reaction diagram
-
-
-
-
?
thiosulfate + 2-mercaptoethanol
?
show the reaction diagram
-
20% of the activity with cyanide
-
-
?
thiosulfate + a thiol
?
show the reaction diagram
-
single displacement mechanism
-
-
?
thiosulfate + benzene thiosulfonate
?
show the reaction diagram
-
-
-
-
?
thiosulfate + borohydride
?
show the reaction diagram
thiosulfate + cyanide
sulfite + thiocyanate
show the reaction diagram
thiosulfate + D,L-homocysteine
?
show the reaction diagram
-
19% of the activity with cyanide
-
-
?
thiosulfate + dihydrolipoate
?
show the reaction diagram
thiosulfate + dithionite
?
show the reaction diagram
thiosulfate + dithiothreitol
?
show the reaction diagram
-
-
-
-
?
thiosulfate + glutathione
?
show the reaction diagram
-
20% of the activity with cyanide
-
-
?
thiosulfate + L-cysteine
?
show the reaction diagram
-
12% of the activity with cyanide
-
-
?
thiosulfate + monothiol
?
show the reaction diagram
-
-
-
-
?
thiosulfate + N-acetyl-L-cysteine
?
show the reaction diagram
-
5% of the activity with cyanide
-
-
?
thiosulfate + sulfite
sulfite + thiosulfate
show the reaction diagram
thiosulfate + thiosulfinate
?
show the reaction diagram
-
-
-
-
?
thiosulfonate + cyanide
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiosulfate + cyanide
sulfite + thiocyanate
show the reaction diagram
additional information
?
-
-
enzyme does not metabolize sulfide. The rate limiting step in sulfide detoxification is oxidation by a sulfide oxidase to thiosulfate
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
one ion per monomer, but not necessary for enzyme activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
2-Naphthalene sulfonate
-
-
2-oxoglutarate
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
Anions
-
CaCl2
Cd2+
-
more than 70% inhibition at 0.1 mM
Cu2+
-
more than 70% inhibition at 0.1 mM
Dinitrobenzene
-
-
-
Dinitrofluorobenzene
-
-
-
dithioerythritol
dithiothreitol
DL-dihydrolipoate
DL-isocitrate
-
-
E-4-ethyl-2-[E-hydroxyimino]-5-nitro-3-hexenamide
-
dithithreitol prevents enzyme inhibition and fully restores enzyme activity
fumarate
-
-
glutathione
-
activation of the enzyme with a maximum at 0.005-0.02 mM, inhibition above
H2O2
-
the less negative isoform is more instable to H2O2
iodoacetamide
-
complete inhibition at 20 mM
iodoacetate
L-cysteine
lipoate
menadione
-
1 h incubation at 0.02 mM leads to loss of the activity of thiosulfate sulfurtransferase by 33%. In addition, 3-mercaptosulfurtransferase and the level of sulfane sulfur and glutathione are decreased
N-bromosuccinimide
-
-
Ni2+
-
more than 70% inhibition at 0.1 mM
oxaloacetate
-
-
p-chloromercuribenzoate
Phenylglyoxal
-
-
pyruvate
-
-
S-nitroso-glutathione
-
dithiothreitol prevents enzyme inhibition and fully restores enzyme activity
S-nitroso-N-acetyl-penicillamine
-
dithithreitol prevents enzyme inhibition and fully restores enzyme activity
SO42-
-
incubation of mitochondria with sulfate causes a significant decrease in activity
Sodium arsenite
-
-
Sodium borohydride
-
pH 8.0
succinate
-
-
sulfhydryl reagents
-
-
Sulfide
-
activating at 0.1 mM, inhibitory above 0.3 mM, in human colon cancer cell line HT-29
sulfite
Thiocyanate
-
-
thiosulfate
Triton X-100
-
slight
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Butyrate
-
significantly increase of enzyme activity and expression in human colon cancer cell line HT-29
dihydrolipoate
-
increases activity at low concentrations
dithioerythritol
-
maximal activation at 0.005 mM, inhibition above
glutathione
-
increases activity at low concentrations
L-cysteine
reduced glutathione
-
enhances activity
Sulfide
-
activating at 0.1 mM, inhibitory above 0.3 mM, in human colon cancer cell line HT-29
sulfite
-
activating effect at high thiosulfate concentrations in p-dimethylaminoazobenzene-treated mice
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.7 - 51.7
3-Mercaptopyruvate
0.03 - 27
CN-
1.7 - 14
cyanide
8.8
H2S
-
20°C, pH 9.2
0.05
Polysulfide
-
pH 9.0, 80°C
-
6.9
tetrathionate
-
pH 9.0, 80°C
0.034
thioredoxin
-
2°C, pH 8.6
0.53 - 603
thiosulfate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7 - 54
3-Mercaptopyruvate
2.8 - 400
thiosulfate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
-
intestine
0.008
-
abomasum
0.012
-
enzyme from muscle, pH 9.2, 37°C
0.014
-
intestine
0.015
-
with H2S as substrate
0.016
-
omasum
0.017
-
spleen
0.018
-
rumen
0.026
-
abomasum
0.029
-
rumen
0.03
-
enzyme from lung, pH 9.2, 37°C
0.033
-
rumen
0.035
-
enzyme from brain, pH 9.2, 37°C
0.047
-
oesophagus
0.069
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of methionine
0.071
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 0.9% NaCl as control; rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiosulfate
0.077
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of cysteine
0.078
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiocystine
0.085
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-4-carboxylic acid
0.092
-
kidney
0.1
-
microsomal rhodanese 28 days after common bile duct ligation
0.11
-
rhodanese activity in muscle of omasum of male and female animals
0.113
-
rhodanese activity in the Ehrlich ascites tumor cells of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-2,4-dicarboxylic acid
0.128
-
stomach
0.158
-
small intestine
0.159
-
microsomal rhodanese 28 days after common bile duct ligation
0.183
-
liver
0.19
-
rhodanese activity in muscle of reticulum of rumen of female animals
0.21
-
rhodanese activity in fundus muscle of abomasum of male animals
0.214
-
liver
0.22
-
rhodanese activity in fundus muscle of abomasum of female animals
0.23
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of cysteine
0.246
-
left colon
0.254
-
rectum
0.289
-
microsomal rhodanese 28 days after apparent operation
0.29
-
rhodanese activity in small intestine of female animals
0.292
-
right colon
0.3
-
rhodanese activity in heart ventricle of female animals
0.314
-
liver
0.34
-
rhodanese activity in heart ventricle of male animals
0.362
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-2,4-dicarboxylic acid
0.374
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of 2-methyl-thiazolidine-4-carboxylic acid
0.376
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of methionine
0.38
-
rhodanese activity in heart atrium of male animals
0.39
-
rhodanese activity in heart atrium of female animals
0.4
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiocystine
0.426
-
kidney
0.476
-
rhodanese activity in the liver of tumor-bearing mouse on the fourth day after intraperitoneal administration of thiazolidine-4-carboxylic acid
0.48
-
enzyme from brain, pH 9.2, 37°C
0.49
-
rhodanese activity in brain of female animals
0.51
-
rhodanese activity in fundus epithelium of abomasum of male animals
0.52
-
rhodanese activity in fundus epithelium of abomasum of female animals
0.57
-
rhodanese activity in pylore epithelium of abomasum of female animals
0.59
-
muscle
0.6
-
rhodanese activity in large intestine of male animals
0.619
-
kidney
0.62
-
rhodanese activity in large intestine of male animals
0.63
-
preparative PAGE
0.67
-
spleen
0.82
-
enzyme from kidney cortex, pH 9.2, 37°C
0.88
-
kidney
0.903
-
liver
0.965
-
enzyme from kidney, pH 9.2, 37°C
0.97
-
rhodanese activity in kidney cortex of female animals
1.03
-
cytosolic rhodanese 42 days after common bile duct ligation
1.06
-
cytosolic rhodanese 28 days after common bile duct ligation
1.08
-
mitochondrial rhodanese 42 days after common bile duct ligation
1.11
-
cytosolic rhodanese 14 days after common bile duct ligation
1.14
-
mitochondrial rhodanese 28 days after common bile duct ligation
1.25
-
liver
1.35
-
cytosolic rhodanese 28 days after common bile duct ligation
1.4
-
enzyme from kidney, pH 9.2, 37°C
1.68
-
mitochondrial rhodanese 28 days after common bile duct ligation
1.83
-
cytosolic rhodanese 7 days after common bile duct ligation
2.18
-
mitochondrial rhodanese 14 days after common bile duct ligation
2.27
-
cytosolic rhodanese 3 days after common bile duct ligation
2.44
-
mitochondrial rhodanese 7 days after common bile duct ligation
2.5
-
cytosolic rhodanese 2 days after common bile duct ligation
2.61
-
cytosolic rhodanese 1 day after common bile duct ligation
2.85
-
cytosolic rhodanese 12 h after common bile duct ligation
2.99
-
rhodanese activity in omasum of male animals
3
-
rhodanese activity in liver of female animals; rhodanese activity in omasum of female animals
3.06
-
cytosolic rhodanese 12 h after apparent operation
3.07
-
cytosolic rhodanese 14 and 42 days after apparent operation
3.08
-
cytosolic rhodanese 3 days after apparent operation
3.09
-
cytosolic rhodanese 7 and 28 days after apparent operation
3.1
-
cytosolic rhodanese 1 and 2 days after apparent operation
3.14
-
rhodanese activity in reticulum of rumen of female animals
3.25
-
mitochondrial rhodanese 3 days after common bile duct ligation
3.63
-
cytosolic rhodanese 28 days after apparent operation
3.82
-
rhodanese activity in epithelium of omasum of female animals
4.02
-
mitochondrial rhodanese 1 day after common bile duct ligation
4.16
-
rhodanese activity in rumen of male animals
4.18
-
mitochondrial rhodanese 12 h after common bile duct ligation
4.23
-
rhodanese activity in rumen of female animals
4.25
-
mitochondrial rhodanese 7 and 42 days after apparent operation
4.26
-
mitochondrial rhodanese 14 and 28 days after apparent operation
4.3
-
mitochondrial rhodanese 2 and 3 days after apparent operation
4.31
-
mitochondrial rhodanese 1 day after apparent operation
4.35
-
mitochondrial rhodanese 12 h after apparent operation
4.64
-
mitochondrial rhodanese 28 days after apparent operation
4.95
-
enzyme from liver, pH 9.2, 37°C
5
-
rhodanese activity in epithelium of reticulum of rumen of female animals
5.08
-
rhodanese activity in epithelium of reticulum of rumen of male animals
5.21
-
enzyme from liver, pH 9.2, 37°C
8.92
-
rhodanese activity in epithelium of rumen of female animals
8.99
-
rhodanese activity in epithelium of rumen of male animals
10.3
-
enzyme from epithelial layer of rumen, pH 9.2, 37°C
15.9
-
Sephadex G-100
16.3
-
enzyme from epithelial layer of rumen, pH 9.2, 37°C
70
-
QAE-Sephadex column II
85.3
-
substrate thiosulfate, pH 9.2, 22°C
225
-
recombinant wild-type enzyme
332
-
wild-type
353
-
Ser-1
389
-
Ser-2
391
-
one additional Arg residue at the 3'-end followed by a TAA stop codon
491
-
C-terminally extended mutant enzyme
575.5
-
R186L
600
-
main fraction of Sephadex G-200 gel chromatography
672.8
-
wild-type
700
-
preparative polyacrylamide gel electrophoresis
720
-
-
730
-
purificated liver rhodanese
818
-
recombinant wild-type
850
-
pH 9.0, 80°C
1071
-
after Sephacryl S-200-HR gel filtration
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
mitochondria
7 - 8.5
-
-
7.5 - 8.5
-
-
8.5 - 10.5
-
strain O90 and strain O77
8.5 - 11.5
-
strain O10
10.5
-
strain O82
11.5
-
strain O80
additional information
-
cytoplasmic enzyme exhibits a more acidic pH-optimum than the mitochondrial enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 10.5
-
pH 5.5: about 45% of maximal activity, pH 10.5: about 95% of maximal activity, pH 11.0: about 15% of maximal activity
6 - 11
-
pH 6.0: about 75% of activity maximum, pH 11.0: about 70% of activity maximum
7
-
no residual activity below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 32
35 - 55
-
strain O90
45
-
strain O10 and strain O82
73
-
mitochondrial and cytosolic enzyme of untreated mice
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 50
-
-
25
-
8% of maximum activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
isoelectrophoresis in polyacrylamide gels, cytosol
5.3
-
isoelectrophoresis in polyacrylamide gels, mitochondria
6.6
-
isoelectric focusing in Phast Gel isoelectric focusing gel with pH range: 3 - 9
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
atrium and ventricle
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information