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IUBMB Comments Isolated from the bacteria Legionella pneumophila and Campylobacter jejuni. Involved in biosynthesis of legionaminic acid, a sialic acid-like derivative that is incorporated into virulence-associated cell surface glycoconjugates which may include lipopolysaccharide (LPS), capsular polysaccharide, pili and flagella.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
CMP-legionaminic acid synthetase, CMP-N,N'-diacetyllegionaminic acid synthetase,
legF ,
neuA ,
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CMP-legionaminic acid synthetase
CMP-N,N'-diacetyllegionaminic acid synthetase
CMP-legionaminic acid synthetase
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CMP-legionaminic acid synthetase
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CMP-N,N'-diacetyllegionaminic acid synthetase
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CMP-N,N'-diacetyllegionaminic acid synthetase
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CMP-N,N'-diacetyllegionaminic acid synthetase
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legF
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neuA
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CTP + N,N'-diacetyllegionaminate = CMP-N,N'-diacetyllegionaminate + diphosphate
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CTP:N,N'-diacetyllegionaminate cytidylyltransferase
Isolated from the bacteria Legionella pneumophila and Campylobacter jejuni. Involved in biosynthesis of legionaminic acid, a sialic acid-like derivative that is incorporated into virulence-associated cell surface glycoconjugates which may include lipopolysaccharide (LPS), capsular polysaccharide, pili and flagella.
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CTP + N,N'-diacetyllegionaminic acid
CMP-N,N'-diacetyllegionaminic acid + diphosphate
CTP + N,N'-diacetyllegionaminic acid
CMP-N,N'-diacetyllegionaminic acid + diphosphate
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CTP + N,N'-diacetyllegionaminic acid
CMP-N,N'-diacetyllegionaminic acid + diphosphate
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CTP + N,N'-diacetyllegionaminic acid
CMP-N,N'-diacetyllegionaminic acid + diphosphate
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CTP + N,N'-diacetyllegionaminic acid
CMP-N,N'-diacetyllegionaminic acid + diphosphate
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x * about 30000, His-tagged protein, SDS-PAGE
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x * about 30000, His-tagged protein, SDS-PAGE
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immobilized metal ion affinity chromatography (Ni2+)
immobilized metal ion affinity chromatography (Ni2+)
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immobilized metal ion affinity chromatography (Ni2+)
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His-tagged protein expressed in Escherichia coli
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His-tagged protein expressed in Escherichia coli BL21(DE3)
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Glaze, P.A.; Watson, D.C.; Young, N.M.; Tanner, M.E.
Biosynthesis of CMP-N,N-diacetyllegionaminic acid from UDP-N,N-diacetylbacillosamine in Legionella pneumophila
Biochemistry
47
3272-3282
2008
Legionella pneumophila, Legionella pneumophila ATCC 33152D
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Schoenhofen, I.; Vinogradov, E.; Whitfield, D.; Brisson, J.; Logan, S.
The CMP-legionaminic acid pathway in Campylobacter: Biosynthesis involving novel GDP-linked precursors
Glycobiology
19
715-725
2009
Campylobacter jejuni, Campylobacter jejuni 11168
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