Information on EC 2.7.7.49 - RNA-directed DNA polymerase and Organism(s) Homo sapiens

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The taxonomic range for the selected organisms is: Homo sapiens

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.7.49
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RECOMMENDED NAME
GeneOntology No.
RNA-directed DNA polymerase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (RNA-directed)
Catalyses RNA-template-directed extension of the 3'- end of a DNA strand by one deoxynucleotide at a time. Cannot initiate a chain de novo. Requires an RNA or DNA primer. DNA can also serve as template. See also EC 2.7.7.7 DNA-directed DNA polymerase.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-38-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
deoxynucleoside triphosphate + DNAn
diphosphate + DNAn+1
show the reaction diagram
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DNA polymerase Pol gamma also catalyzes reverse transcription with a slightly higher efficiency than HIV-1 reverse transcriptase. RNA-primed DNA synthesis activity is required for initiation of mtDNA replication. Poly gamma holoenzyme is capable of performing this reaction at a physiologically releavant rate
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
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maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 with 50 mM KCl
Mg2+
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divalent metal ion required, enzyme has a preference for Mg2+ over Mn2+. Activity on poly(rA)*oligo(dT)12 is almost 3fold higher with Mg2+ than with Mn2+
Mn2+
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divalent metal ion required, enzyme has a preference for Mg2+ over Mn2+. Activity on poly(rA)*oligo(dT)12 is almost 3fold higher with Mg2+ than with Mn2+. Maximal dTTP incorporation into poly(rA)*oligo(dT)12 occurs between 0.3 and 0.6 mM MnCl2
NaCl
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maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 at 100 mM NaCl
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-epigallocatechin-3-gallate
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exposure to (-)-epigallocatechin-3-gallate reduces cellular proliferation and induced apoptosis in both MCF-7 and HL60 cells in vitro, although TERT mRNA expression is decreased only in MCF-7 cells
early growth response-1
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overexpressio nof early growth response-1 decreases TERT protein production as well TERT mRNA transcription
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Mn2+
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above 1 mM MnCl2
stavudine
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potent inhibitor
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
betapapillomavirus E6 protein 16E6
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activates telomerase, NFX1-91 protein is important for telomerase activation by betapapillomavirus E6 protein 16E6
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betapapillomavirus E6 protein 38E6
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activates telomerase
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betapapillomavirus E6 protein E6AP
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important for telomerase activation by betapapillomavirus E6 protein 16E6
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dithiothreitol
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maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 with 10 mM dithiothreitol
Epidermal growth factor
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exogenous epidermal growth factor activates TERT gene transcription, epidermal growth factor-induced TERT activity is ERK 1/2-dependent
NFX-91
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complex formation between betapapillomavirus E6 proteins E6, E6AP, and NFX1-91 is a critical step in mediating telomerase activation
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pontin
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pontin interacts with TERT and dyskerin and is critical for telomerase activity and for the accumulation of TERC and dyskerin
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reptin
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reptin interacts with TERT and dyskerin and is critical for telomerase activity and for the accumulation of TERC and dyskerin
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additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23 - 47
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23°C: about 30% of maximal activity, 47°C: about 15% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Homo sapiens;
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
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monomer
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ribonucleoprotein
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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heat sensitive enzyme
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, 50% glycerol, stable for more than 12 months
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-85 to -75°C, in frozen cells or tissues, at least one year, remains stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ectopic expression in the heterohybridoma cell line K6H6/B5
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human LINE-1 ORF2, which encodes reverse transcriptase, is inserted into a baculovirus shuttle vector and expressed in SF21 cells
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine