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EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ATP:thiamin-diphosphate phosphotransferase, kinase (phosphorylating), thiamin diphosphate, kinase, thiamin diphosphate (phosphorylating), protein bound thiamin diphosphate:ATP phosphoryltransferase, TDP kinase, thiamin diphosphate kinase, thiamin diphosphate phosphotransferase, thiamin pyrophosphate kinase, thiamin-diphosphate kinase, thiamine diphosphate kinase,
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ATP:thiamin-diphosphate phosphotransferase
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kinase (phosphorylating), thiamin diphosphate
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kinase, thiamin diphosphate (phosphorylating)
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protein bound thiamin diphosphate:ATP phosphoryltransferase
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thiamin diphosphate kinase
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thiamin diphosphate phosphotransferase
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thiamin pyrophosphate kinase
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thiamin-diphosphate kinase
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thiamine diphosphate kinase
thiamine pyrophosphate-ATP phosphoryltransferase
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thiamine diphosphate kinase
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thiamine diphosphate kinase
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ATP + thiamine diphosphate = ADP + thiamine triphosphate
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phospho group transfer
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ATP:thiamine-diphosphate phosphotransferase
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ATP + thiamine diphosphate
ADP + thiamine triphosphate
CTP + thiamine diphosphate
CDP + thiamine triphosphate
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6% of the activity with ATP
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additional information
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enzymatic synthesis of thiamine triphosphate in Escherichia coli
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ATP + thiamine diphosphate
ADP + thiamine triphosphate
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ATP + thiamine diphosphate
ADP + thiamine triphosphate
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ATP + thiamine diphosphate
ADP + thiamine triphosphate
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ATP + thiamine diphosphate
ADP + thiamine triphosphate
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ATP + thiamine diphosphate
ADP + thiamine triphosphate
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r
ATP + thiamine diphosphate
ADP + thiamine triphosphate
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Co2+
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about 50% of the activation with Mg2+
Fe2+
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30 mM FeCl2, maximal activation of approximately 30%
Fe3+
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30 mM FeCl32, maximal activation of approximately 30%
Mn2+
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about 50% of the activation with Mg2+
Mg2+
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required
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HgCl2
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10 mM, complete inhibition
NEM
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10 mM, 64% inhibition
PCMB
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0.5 mM, 79.5% inhibition
PCMB
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10 mM, complete inhibition
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Creatine
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the enzyme is dependent on creatine
additional information
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a low molecular weight cofactor is required
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Leigh Disease
Studies on ATP: thiamine diphosphate phosphotransferase activity in rat brain.
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1.1
thiamine diphosphate
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0.01
ATP
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additional information
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thiamine triphoshate accumulation in response to amino acid starvation, controverse mechanism of synthesis dicussed, adenylate kinase-independent accumulation of thiamine triphosphate, heat-sensitive CV2 strain of Escherichia coli accumulates thiamine triphosphate despite full inactivation of adenylate cyclase and subsequent decrease of cellular ATP, ATP-dependent mechanisms for thiamine triphosphate synthesis not favoured since large accumulation also observed during energy stress where ATP is low
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7.5
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in Tris-HCl buffer slightly higher activity than in phosphate buffer
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5.1
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isoelectric focusing
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brenda
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brenda
BL21 strain and heat-sensitive CV2, CGSC 4682 strain
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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cortex
brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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primarily localized in
brenda
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brenda
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brenda
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Itokawa, Y.; Cooper, J.R.
The enzymatic synthesis of triphosphothiamin
Biochim. Biophys. Acta
158
180-182
1968
Rattus norvegicus
brenda
Cooper, J.R.; Nishino, K.
Enzymatic synthesis of thiamin triphosphate
Methods Enzymol.
122
24-29
1986
Bos taurus
brenda
Koyama, S.; Egi, Y.; Shikata, H.; Yamada, K.; Kawasaki, T.
Existence in animal tissues of adenosine triphosphate thiamin diphosphate phosphotransferase [EC 2.7.4.15]
Biochem. Int.
11
371-380
1985
Cavia porcellus
brenda
Shikata, H.; Koyama, S.; Egi, Y.; Yamada, K.; Kawasaki, T.
Identification of creatine as a cofactor of thiamin-diphosphate kinase
FEBS Lett.
201
101-104
1986
Sus scrofa
brenda
Ruenwongsa, P.; Cooper, J.R.
The role of bound thiamine pyrophosphate in the synthesis of thiamine triphosphate in rat liver
Biochim. Biophys. Acta
482
64-70
1977
Rattus norvegicus
brenda
Nishino, K.; Itokawa, Y.; Nishino, N.; Piros, K.; Cooper, J.R.
Enzyme system involved in the synthesis of thiamin triphosphate. I. Purification and characterization of protein-bound thiamin diphosphate: ATP phosphoryltransferase
J. Biol. Chem.
258
11871-11878
1983
Bos taurus
brenda
Yamaguchi, T.; Uchimura, K.; Mishiro, N.; Watanabe, K.
Evidence for the presence of thiamin diphosphate kinase in human erythrocytes
JPN. J. Toxicol. Environ. Health
42
524-528
1996
Homo sapiens
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brenda
Gigliobianco, T.; Lakaye, B.; Makarchikov, A.F.; Wins, P.; Bettendorff, L.
Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coli
BMC Microbiol.
8
16
2008
Escherichia coli
brenda
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