We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.7.4.15 - thiamine-diphosphate kinase for references in articles please use BRENDA:EC2.7.4.15
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ATP:thiamin-diphosphate phosphotransferase, kinase (phosphorylating), thiamin diphosphate, kinase, thiamin diphosphate (phosphorylating), protein bound thiamin diphosphate:ATP phosphoryltransferase, TDP kinase, thiamin diphosphate kinase, thiamin diphosphate phosphotransferase, thiamin pyrophosphate kinase, thiamin-diphosphate kinase, thiamine diphosphate kinase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP:thiamin-diphosphate phosphotransferase
-
-
-
-
kinase (phosphorylating), thiamin diphosphate
-
-
-
-
kinase, thiamin diphosphate (phosphorylating)
-
-
-
-
protein bound thiamin diphosphate:ATP phosphoryltransferase
-
-
-
-
thiamin diphosphate kinase
-
-
-
-
thiamin diphosphate phosphotransferase
-
-
-
-
thiamin pyrophosphate kinase
-
-
-
-
thiamin-diphosphate kinase
-
-
-
-
thiamine diphosphate kinase
thiamine pyrophosphate-ATP phosphoryltransferase
-
-
-
-
thiamine diphosphate kinase
-
-
-
-
thiamine diphosphate kinase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + thiamine diphosphate = ADP + thiamine triphosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
phospho group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP:thiamine-diphosphate phosphotransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + thiamine diphosphate
ADP + thiamine triphosphate
CTP + thiamine diphosphate
CDP + thiamine triphosphate
-
6% of the activity with ATP
-
-
?
additional information
?
-
-
enzymatic synthesis of thiamine triphosphate in Escherichia coli
-
-
-
ATP + thiamine diphosphate
ADP + thiamine triphosphate
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
-
-
-
-
?
ATP + thiamine diphosphate
ADP + thiamine triphosphate
-
-
-
-
r
ATP + thiamine diphosphate
ADP + thiamine triphosphate
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Co2+
-
about 50% of the activation with Mg2+
Fe2+
-
30 mM FeCl2, maximal activation of approximately 30%
Fe3+
-
30 mM FeCl32, maximal activation of approximately 30%
Mn2+
-
about 50% of the activation with Mg2+
Mg2+
-
required
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
HgCl2
-
10 mM, complete inhibition
NEM
-
10 mM, 64% inhibition
PCMB
-
0.5 mM, 79.5% inhibition
PCMB
-
10 mM, complete inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Creatine
-
the enzyme is dependent on creatine
additional information
-
a low molecular weight cofactor is required
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.1
thiamine diphosphate
-
-
0.01
ATP
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
thiamine triphoshate accumulation in response to amino acid starvation, controverse mechanism of synthesis dicussed, adenylate kinase-independent accumulation of thiamine triphosphate, heat-sensitive CV2 strain of Escherichia coli accumulates thiamine triphosphate despite full inactivation of adenylate cyclase and subsequent decrease of cellular ATP, ATP-dependent mechanisms for thiamine triphosphate synthesis not favoured since large accumulation also observed during energy stress where ATP is low
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.5
-
in Tris-HCl buffer slightly higher activity than in phosphate buffer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.1
-
isoelectric focusing
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
BL21 strain and heat-sensitive CV2, CGSC 4682 strain
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
-
-
brenda
-
cortex
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
primarily localized in
brenda
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Itokawa, Y.; Cooper, J.R.
The enzymatic synthesis of triphosphothiamin
Biochim. Biophys. Acta
158
180-182
1968
Rattus norvegicus
brenda
Cooper, J.R.; Nishino, K.
Enzymatic synthesis of thiamin triphosphate
Methods Enzymol.
122
24-29
1986
Bos taurus
brenda
Koyama, S.; Egi, Y.; Shikata, H.; Yamada, K.; Kawasaki, T.
Existence in animal tissues of adenosine triphosphate thiamin diphosphate phosphotransferase [EC 2.7.4.15]
Biochem. Int.
11
371-380
1985
Cavia porcellus
brenda
Shikata, H.; Koyama, S.; Egi, Y.; Yamada, K.; Kawasaki, T.
Identification of creatine as a cofactor of thiamin-diphosphate kinase
FEBS Lett.
201
101-104
1986
Sus scrofa
brenda
Ruenwongsa, P.; Cooper, J.R.
The role of bound thiamine pyrophosphate in the synthesis of thiamine triphosphate in rat liver
Biochim. Biophys. Acta
482
64-70
1977
Rattus norvegicus
brenda
Nishino, K.; Itokawa, Y.; Nishino, N.; Piros, K.; Cooper, J.R.
Enzyme system involved in the synthesis of thiamin triphosphate. I. Purification and characterization of protein-bound thiamin diphosphate: ATP phosphoryltransferase
J. Biol. Chem.
258
11871-11878
1983
Bos taurus
brenda
Yamaguchi, T.; Uchimura, K.; Mishiro, N.; Watanabe, K.
Evidence for the presence of thiamin diphosphate kinase in human erythrocytes
JPN. J. Toxicol. Environ. Health
42
524-528
1996
Homo sapiens
-
brenda
Gigliobianco, T.; Lakaye, B.; Makarchikov, A.F.; Wins, P.; Bettendorff, L.
Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coli
BMC Microbiol.
8
16
2008
Escherichia coli
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
2.7.4.15 thiamine-diphosphate kinase
more
top
Information
