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EC Tree
IUBMB Comments dTMP and dAMP can act as acceptors; dATP can act as donor.
The expected taxonomic range for this enzyme is: Escherichia coli
Synonyms
deoxynucleotide kinase,
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deoxynucleotide kinase
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kinase, deoxynucleotide (phosphorylating, T2-induced)
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ATP + dGMP (or dTMP) = ADP + dGDP (or dTDP)
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phospho group transfer
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ATP:(d)NMP phosphotransferase
dTMP and dAMP can act as acceptors; dATP can act as donor.
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ATP + 5-hydroxymethyl-dCMP
ADP + 5-hydroxymethyl-dCDP
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phosphorylation at about half the rate of dGMP or dTMP
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r
ATP + dTMP
ADP + dTDP
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as good as dGMP
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r
dATP + dGMP
dADP + dGDP
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phosphorylation at about 80-90% the rate of ATP
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r
dATP + dTMP
dADP + dTDP
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phosphorylation at about 80-90% the rate of ATP
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r
additional information
?
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poor substrates are dUMP or 5-methyl-dCMP, no donor substrates are GTP, CTP, dGTP, dCTP, or dTTP, no acceptor substrates are dCMP, dAMP, GMP, or UMP
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?
ATP + dGMP
ADP + dGDP
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as good as dTMP
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r
ATP + dGMP
ADP + dGDP
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or dTMP
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r
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ATP + dGMP
ADP + dGDP
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or dTMP
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r
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Ca2+
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requirement, about 60% as effective as Mn2+, dGMP as substrate
Mg2+
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requirement, about 90% as effective as Mn2+, dGMP as substrate
Mn2+
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requirement, dGMP as substrate
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5-hydroxymethyl-dCMP
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dGMP or dTMP as substrate
dGMP
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dTMP or 5-hydroxymethyl-dCMP as substrate
dTMP
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dGMP or 5-hydroxymethyl-dCMP as substrate
additional information
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tryptic digestion inactivates
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0.056
5-hydroxymethyl-dCMP
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pH 7.4, 37°C
0.085
dGMP
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pH 7.4, 37°C
0.278 - 0.313
dTMP
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pH 7.4, 37°C
0.82
ATP
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pH 7.4, 37°C, with dTMP
1.25
ATP
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pH 7.4, 37°C, with dGMP
5
ATP
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pH 7.4, 37°C, with 5-hydroxymethyl-dCMP
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0.04 - 0.044
5-hydroxymethyl-dCMP
0.04
5-hydroxymethyl-dCMP
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pH 7.4, 37°C, dTMP is substrate
0.044
5-hydroxymethyl-dCMP
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pH 7.4, 37°C, dGMP is substrate
0.064
dGMP
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pH 7.4, 37°C, dTMP is substrate
1.28
dGMP
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pH 7.4, 37°C, 5-hydroxymethyl-dCMP is substrate
0.25
dTMP
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pH 7.4, 37°C, 5-hydroxymethyl-dCMP is substrate
0.314
dTMP
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pH 7.4, 37°C, dGMP is substrate
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8.6
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5-hydroxymethyl-dCMP
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7 - 9.3
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pH 7: about 75% of maximal activity of 5-hydroxymethyl-dCMP, 84% of maximal activity dTMP or 87% of maximal activity of dGMP, pH 9.3: about 79% of maximal activity of dTMP, 83% of maximal activity of dGMP or 90% of maximal activity of 5-hydroxymethyl-dCMP
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infected with bacteriophage T2
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brenda
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4
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3 min, about 60% loss of activity at 37°C
642541
4.4
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37°C, 3 min, about 30% loss of activity, and about 45% loss of activity within 6 min
642541
4.7
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15 min, about 20% loss of activity at 37°C
642541
5.4
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15 min stable at 37°C
642541
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-10°C, crude preparation, at least a year
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4°C, partially purified preparation, at least 2 months
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at -10°C to 4°C, 1 mM EDTA, up to 20% loss of activity within 6 months
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infected with bacteriophage T2
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Bello, L.J.; Bessman, M.J.
The enzymology of virus-infected bacteria, IV. Purification and properties of the deoxynucleotide kinase induced by bacteriophage T2
J. Biol. Chem.
238
1777-1787
1963
Escherichia coli
brenda
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2.7.4.12 T2-induced deoxynucleotide kinase
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