Information on EC 2.7.3.4 - taurocyamine kinase

Substrates: cytoplasmic TK shows activity only for D-lombricine and taurocyamine (the second activity being in the range of 15% to that for the main substrate), no activity for creatine
Products: -

additional information

Substrates: no activity for creatine
Products: -

additional information

Substrates: no activity for creatine
Products: -

additional information

Substrates: the mitochondrial isoform of MiTK shows high activity for glycocyamine (48%), in addition to D-lombricine (80%), the MiTK also shows weak but significant activity for L-arginine (0.2%)
Products: -

additional information

additional information

Substrates: residues R58, I60 and Y84 of domain 1, and H60, I63 and Y87 of domain 2 participate in binding taurocyamine. Recombinant CsTK enzyme protein has low sensitivity and specificity toward Clonorchis sinensis and platyhelminth-infected human sera on ELISA
Products: -

additional information

additional information

Phytophthora infestans

722083

Substrates: no activity with L-arginine, D-arginine, creatine, glycocyamine, gamma-guanidinobutyric acid, homoarginine, methylguanidine and L-acetylguanidine
Products: -

additional information

Substrates: the phosphagen kinases in the invertebrates are observed to utilize other guanidino substrates. These include arginine, glycocyamine, taurocyamine, lombricine, hypotaurocyamine, and opheline. Analysis of phosphagen formation by 31P NMR, overview
Products: -

additional information

additional information

Substrates: no kinase activity with the more ubiquitous guanidinium substrates, creatine or arginine
Products: -

additional information

Substrates: no kinase activity with the more ubiquitous guanidinium substrates, creatine or arginine
Products: -

additional information

additional information

Substrates: no kinase activity with the more ubiquitous guanidinium substrates, creatine or arginine
Products: -

additional information

Schistosoma mansoni

P16641

690293

Substrates: recombinant enzyme shows catalytic activity with several guanidylic compounds, the highest activity being measured with taurocyamine, taurocyamine may not be the physiological substrate and that Schistosoma mansoni may use another phosphagen
Products: -

additional information

Schistosoma mansoni

690293

show all columns Go to Natural Substrate Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

ATP + D-lombricine

ADP + N-phospho-D-lombricine

Substrates: -
Products: -

ATP + glycocyamine

ADP + N-phosphoglycocyamine

3 entries

ATP + taurocyamine

ADP + N-phosphotaurocyamine

14 entries

additional information

9 entries

ATP + glycocyamine

ADP + N-phosphoglycocyamine

Substrates: -
Products: -

ATP + glycocyamine

ADP + N-phosphoglycocyamine

Substrates: low activity
Products: -

ATP + glycocyamine

ADP + N-phosphoglycocyamine

Substrates: low activity
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Substrates: key role in the interconnection between energy production and utilization in animals
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Substrates: key role in the interconnection between energy production and utilization in animals
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Paragonimus iloktsuenensis

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Paragonimus ohirai

Paragonimus skrjabini miyazakii

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

738180

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Substrates: -
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Riftia pachyptila

Q4AEC7, Q4AEC8

Substrates: key role in the interconnection between energy production and utilization in animals
Products: -

ATP + taurocyamine

ADP + N-phosphotaurocyamine

Schistosoma mansoni

P16641

738646

Substrates: -
Products: -

additional information

Substrates: cytoplasmic TK shows activity only for D-lombricine and taurocyamine (the second activity being in the range of 15% to that for the main substrate), no activity for creatine
Products: -

additional information

Substrates: cytoplasmic TK shows activity only for D-lombricine and taurocyamine (the second activity being in the range of 15% to that for the main substrate), no activity for creatine
Products: -

additional information

Substrates: no activity for creatine
Products: -

additional information

Substrates: no activity for creatine
Products: -

additional information

additional information

additional information

additional information

additional information

show all columns Go to Cofactor Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

ADP

ATP

ATP

ATP

703716, 738180

ATP

show all columns Go to Metals and Ions Search

ATP

ATP

ATP

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Mg2+

5 entries

Mg2+

required, maximal activity at 10 mM

Mg2+

required

Mg2+

show all columns Go to Inhibitor Search

required

Mg2+

required

Mg2+

dependent on

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

Chloroacetophenone

monoiodoacetate

NEM

PCMB

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

DISEASE

TITLE OF PUBLICATION

LINK TO PUBMED

Schistosomiasis

Phosphagen kinase in Schistosoma japonicum: II. Determination of amino acid residues essential for substrate catalysis using site-directed mutagenesis.

24815317

show all columns Go to KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1.2

ADP

pH 7.2, 25°C

0.46 - 4.6

ATP

7 entries

6.1

glycocyamine

recombinant enzyme, pH 9.0, 30°C

0.83

N-phosphotaurocyamine

pH 7.2, 25°C

0.1

N-taurocyamine

pH 8.0, 25°C

0.082 - 33.44

Taurocyamine

30 entries

additional information

4 entries

0.46

ATP

recombinant domain D1, pH 8.0, 25°C

0.75

ATP

recombinant domain D2, pH 8.0, 25°C

0.78

ATP

recombinant full-length enzyme, pH 8.0, 25°C

0.98

ATP

1.8

ATP

recombinant enzyme, pH 9.0, 30°C, with taurocyamine

3.3

ATP

pH 8.0, 25°C

4.6

ATP

recombinant enzyme, pH 9.0, 30°C, with glycocyamine

0.082

Taurocyamine

mutant enzyme K95Y, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.14

Taurocyamine

mutant enzyme T68A/K95Y, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.153

Taurocyamine

mutant enzyme K69A/K95Y, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.184

Taurocyamine

mutant enzyme H67A/K95Y, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.205

Taurocyamine

mutant enzyme K95A, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.217

Taurocyamine

mutant enzyme K69R/K95Y, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.269

Taurocyamine

Phytophthora infestans

isoform PK1, in 100 mM Tris-HCl, at pH 8.0 and 25°C

722083

0.278

Taurocyamine

mutant enzyme K95I, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.287

Taurocyamine

mutant enzyme K95R, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.313

Taurocyamine

mutant enzyme K95H, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.332

Taurocyamine

mutant enzyme T70A/K95Y, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.35

Taurocyamine

recombinant domain D1, pH 8.0, 25°C

0.48

Taurocyamine

recombinant domain D2, pH 8.0, 25°C

0.49

Taurocyamine

recombinant full-length enzyme, pH 8.0, 25°C

0.52

Taurocyamine

recombinant enzyme, pH 9.0, 30°C

0.57

Taurocyamine

0.603

Taurocyamine

mutant enzyme V71A/K95Y, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.718

Taurocyamine

mutant enzyme T68A, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.742

Taurocyamine

mutant enzyme K69R, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.813

Taurocyamine

mutant enzyme H67A, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.881

Taurocyamine

0.913

Taurocyamine

wild type enzyme, in 100 mM Tris-HCl, at pH 8.0 and 25°C

0.969

Taurocyamine

Phytophthora infestans

isoform PK2, in 100 mM Tris-HCl, at pH 8.0 and 25°C

722083

1.228

Taurocyamine

mutant enzyme K69A, in 100 mM Tris-HCl, at pH 8.0 and 25°C

1.868

Taurocyamine

mutant enzyme T70A, in 100 mM Tris-HCl, at pH 8.0 and 25°C

2.091

Taurocyamine

mutant enzyme V71A, in 100 mM Tris-HCl, at pH 8.0 and 25°C

2.12

Taurocyamine

Riftia pachyptila

Q4AEC7, Q4AEC8

4.01

Taurocyamine

13.28

Taurocyamine

mutant enzyme K95E, in 100 mM Tris-HCl, at pH 8.0 and 25°C

33.44

Taurocyamine

additional information

comparisons of wild-type and mutant enzyme kinetics, overview

additional information

comparisons of wild-type and mutant enzyme kinetics, overview

additional information

steady-state kinetic analysis, overview. Analysis of quaternary structure and cooperativity in ligand-binding by ITC: absence of inter-subunit cooperativity in forming the PsTKTSAC

additional information

steady-state kinetic analysis, overview. Analysis of quaternary structure and cooperativity in ligand-binding by ITC: absence of inter-subunit cooperativity in forming the PsTKTSAC

show all columns Go to Turnover Number Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

4.5 - 22.59

ATP

3 entries

1.4

glycocyamine

recombinant enzyme, pH 9.0, 30°C

4.5 - 94.1

Taurocyamine

7 entries

4.5

ATP

recombinant domain D2, pH 8.0, 25°C

17.78

ATP

recombinant full-length enzyme, pH 8.0, 25°C

22.59

ATP

recombinant domain D1, pH 8.0, 25°C

4.5

Taurocyamine

recombinant domain D2, pH 8.0, 25°C

9.43

Taurocyamine

12.5

Taurocyamine

Riftia pachyptila

Q4AEC7, Q4AEC8

14.3

Taurocyamine

17.78

Taurocyamine

recombinant full-length enzyme, pH 8.0, 25°C

22.59

Taurocyamine

recombinant domain D1, pH 8.0, 25°C

94.1

Taurocyamine

recombinant enzyme, pH 9.0, 30°C

show all columns Go to kcat/KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.23

glycocyamine

recombinant enzyme, pH 9.0, 30°C

58.67 - 180

Taurocyamine

2 entries

58.67

Taurocyamine

180

Taurocyamine

recombinant enzyme, pH 9.0, 30°C

show all columns Go to Specific Activity Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.298

arginine

0.855

lombricine

1.32

glycocyamine

10.4

taurocyamine

1283

17.82

taurocyamine

2.541

lombricine

28.71

taurocyamine

3.25

lombricine

3.65

glycocyamine

4.04

taurocyamine

5.16

lombricine

show all columns Go to pH Optimum Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6.8

reaction with phosphotaurocyamine or hypophosphotaurocyamine

7.2

synthesis of ATP

8.5

reaction with hypotaurocyamine

642487

2 entries

show all columns Go to pH Range Search

synthesis of phosphotaurocyamine

assay at

reaction with taurocyamine

assay at

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6.2 - 8

pH 6.2: about 30% of maximal activity, pH 8.0: about 50% of maximal activity, synthesis of ATP

7.2 - 8.7

pH 7.2: about 40% of maximal activity, pH 8.7: about 40% of maximal activity, synthesis of phosphotaurocyamine

7.7 - 9.5

2 entries

7.7 - 9.5

pH 7.7: about 60% of maximal activity, pH 9.5: about 60% of maximal activity, synthesis of hypophosphotaurocyamine

642487

7.7 - 9.5

pH 7.7: about 50% of maximal activity, pH 9.5: about 70% of maximal activity, synthesis of phosphotaurocyamine

642487

show all columns Go to Temperature Optimum Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

assay at

assay at

show all columns Go to Temperature Range Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

20 - 45

20°C: 70% of maximal activity, 45°C: less than 45% of maximal activity

show all columns Go to Pi Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

pI VALUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

7.39

sequence calculation, D2 domain

7.62

calculated

7.88

sequence calculation, full-length enzyme

7.9

calculated from amino acid sequence

7.98

calculated

8.09

sequence calculation, D1 domain

8.55

calculated

additional information

separation of 8 bands between pH 6.2 and pH 7.8 with taurocyamine kinase activity, the highest activity appears at pH 7.3

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

6 entries