Information on EC 2.7.11.23 - [RNA-polymerase]-subunit kinase and Organism(s) Homo sapiens

for references in articles please use BRENDA:EC2.7.11.23
Word Map on EC 2.7.11.23
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
This record set is specific for:
Homo sapiens


The taxonomic range for the selected organisms is: Homo sapiens

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.11.23
-
RECOMMENDED NAME
GeneOntology No.
[RNA-polymerase]-subunit kinase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:[DNA-directed RNA polymerase] phosphotransferase
The enzyme appears to be distinct from other protein kinases. It brings about multiple phosphorylations of the unique C-terminal repeat domain of the largest subunit of eukaryotic DNA-directed RNA polymerase (EC 2.7.7.6). The enzyme does not phosphorylate casein, phosvitin or histone.
CAS REGISTRY NUMBER
COMMENTARY hide
122097-00-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the human orthologue of Saccharomyces cerevisiae Ctk1 is one or both of the isozyme pair, hCDK12-hCDK13; the human orthologue of Saccharomyces cerevisiae Ctk1 is one or both of the isozyme pair, hCDK12-hCDK13
malfunction
metabolism
-
DYRK1A is a dosage-sensitive protein kinase that fulfills key roles during development and in tissue homeostasis. DYRK1A-bound promoters show a higher occurrence of activating chromatin modifications when compared with genes that do not associate with DYRK1A, including high levels of H3K4me3 and H3K4me2 surrounding TSSs or elevated H3K36me3 in the gene body
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + casein
ADP + phosphocasein
show the reaction diagram
-
phosphorylated at about 30% the rate of RNA-polymerase II subunit
-
-
?
ATP + CTD-containing fusion proteins
ADP + ?
show the reaction diagram
ATP + DYRKtide
ADP + phospho-DYRKtide
show the reaction diagram
-
-
-
-
?
ATP + GST-tagged yCTD fusion protein
?
show the reaction diagram
hCDK13 purified from nuclear extracts displays CTD kinase activity in vitro with recombinant GST-tagged yeast CTD fusion proteins as substrate, hyperphosphorylation of the CTD by hCDK13 kinase
-
-
?
ATP + histone H1
ADP + phosphohistone H1
show the reaction diagram
-
-
-
-
?
ATP + HIV Tat
ADP + phosphorylated HIV-Tat
show the reaction diagram
ATP + RNA polymerase II
?
show the reaction diagram
ATP + RNA polymerase II C-terminal subunit
ADP + phosphoylated RNA polymerase II C-terminal subunit
show the reaction diagram
ATP + synthetic peptides
ADP + ?
show the reaction diagram
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
show the reaction diagram
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
show the reaction diagram
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
show the reaction diagram
dATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
dADP + ?
show the reaction diagram
-
-
-
-
?
GTP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
GDP + ?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + HIV Tat
ADP + phosphorylated HIV-Tat
show the reaction diagram
-
-
-
-
?
ATP + RNA polymerase II C-terminal subunit
ADP + phosphoylated RNA polymerase II C-terminal subunit
show the reaction diagram
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
show the reaction diagram
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
show the reaction diagram
P49336, Q9H4B4
only Rpb1, the largest subunit of RNAPII evolved a unique, highly repetitive carboxy-terminal domain, termed CTD. Dynamic phosphorylation patterns of serine residues in the CTD during gene transcription. Also phosphorylation of threonine 4 and tyrosine 1 in the CTD. CTD is composed of multiple tandem heptapeptides with the evolutionary conserved consensus motif Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-(5-isoquinolinesulfonyl)-2-methylpiperazine
5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole
8-(methylthio)-4,5-dihydrothieno[3',4':5,6]benzoisoxazole-6-carboamide
-
KM05283, CTD kinase inhibitor
harmine
-
-
roscovitine
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cyclin H
-
required by Cdk7
-
cyclin T1
-
DNA
-
activation
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03 - 0.06
ATP
0.15
Synthetic peptide
-
27°C, pH 7.5
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27 - 28
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
foreskin fibroblasts
Manually annotated by BRENDA team
-
fibrosarcoma cell line
Manually annotated by BRENDA team
additional information
-
HFF cell
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
67000
-
1 * 67000 + 1 * 83000, component B, SDS-PAGE
83000
-
1 * 67000 + 1 * 83000, component B, SDS-PAGE
180000
-
gel filtration, enzyme component B
340000
-
gel filtration, enzyme component A
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
1 * 67000 + 1 * 83000, component B, SDS-PAGE
additional information
-
the three CTD kinase isozymes possess CDK-cyclin pairs, and TFIIH additionally forms a preinitiation complex, overview
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, glycerol, 20% v/v
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native hCDK13 from nuclear extracts of HeLa cells by Ion exchange chromatography and affinity chromatography, recombinant His-tagged chimeric mutant Ctk1/13 kinase from Saccharomyces cerevisiae strain FY23 by cation exchange chromatography and nickel affinity chromatography; recombinant His-tagged chimeric mutant Ctk1/12 kinase from Saccharomyces cerevisiae strain FY23 by cation exchange chromatographyand nickel affinity chromatography
partial
-
partial; two kinases: CDTK1 and CDTK2
-
two components: A and B
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA copy of cdk9HA placed into a lentiviral vector where its transcription is driven by an EF1alpha promoter, overexpression in HFF cells; overexpression of HA-tagged cdk9 in 293FT cells
-
DG75 cells transfected with pcDNA3-K41M, pFLAG-CMV2-D173A or pFLAG-CMV2-D167N
-
hCDK12, recombinant expression of the His-tagged chimeric mutant Ctk1/12 kinase in Saccharomyces cerevisiae strain FY23; hCDK13, recombinant expression of the His-tagged chimeric mutant Ctk1/13 kinase in Saccharomyces cerevisiae strain FY23
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D167N
-
dominant-negative mutant of CDK9, inhibits transcriptional activation by EBNA 2
D173A
-
CDK8 mutant, shows no effect on basal or EBNA 2-activated transcription
K41M
-
CDK7 mutant, both EBNA 2-activated and basal transcription from Cp reporter construct are not particularly dependent on CDK7 kinase activity with only small effects observed in the presence of high levels of mutant K41M
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
IE2 and multiple cellular factors needed for viral RNA synthesis accumulate within the first 8 h at the viral transcriptosome, functional cdk activity is required for the specific recruitment of cdk7 and cdk9 during this time interval