Information on EC 2.7.11.1 - non-specific serine/threonine protein kinase and Organism(s) Drosophila melanogaster

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Drosophila melanogaster


The taxonomic range for the selected organisms is: Drosophila melanogaster

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.11.1
-
RECOMMENDED NAME
GeneOntology No.
non-specific serine/threonine protein kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a protein = ADP + a phosphoprotein
show the reaction diagram
catalytic mechanism and regulation of PKN
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (non-specific)
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
CAS REGISTRY NUMBER
COMMENTARY hide
191808-15-8
phosphoinositide dependent protein kinase 1
340830-03-7
-
37278-10-7
-
377752-08-4
ribosomal protein S6 kinase 2
389133-24-8
ribosomal S6 kinase 3
52660-18-1
casein kinase, protein kinase CK2
9026-43-1
this CAS Reg. No. encompasses a great variety of protein kinases including the serine/threonine specific kinases
90698-26-3
ribosomal protein S6 kinase 1
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
casein kinase 1 alpha gene is developmentally regulated and the kinase activity of the protein is induced by DNA damage. Possible requirement in mechanisms associated with DNA repair during early embryogenesis
-
-
?
ATP + adenomatous polyposis coli protein
ADP + phosphorylated adenomatous polyposis coli protein
show the reaction diagram
-
i.e. APC, APC is phosphorylated in vitro by CKIdelta and CKIepsilon
-
-
?
ATP + beta-catenin
ADP + phosphorylated beta-catenin
show the reaction diagram
-
phosphorylation at Ser45, priming GSK3beta phosphorylation
-
-
?
ATP + protein Cubitus interruptus
ADP + phosphorylated protein Cubitus interruptus
show the reaction diagram
ATP + protein LRP6
ADP + phosphorylated protein LRP6
show the reaction diagram
-
CKIgamma
-
-
?
ATP + protein TCF3
ADP + phosphorylated protein TCF3
show the reaction diagram
-
CKIepsilon
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
enzyme is essential for eye and embryonic development
-
-
?
casein + ATP
phosphorylated casein + ADP
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
casein kinase 1 alpha gene is developmentally regulated and the kinase activity of the protein is induced by DNA damage. Possible requirement in mechanisms associated with DNA repair during early embryogenesis
-
-
?
ATP + adenomatous polyposis coli protein
ADP + phosphorylated adenomatous polyposis coli protein
show the reaction diagram
-
i.e. APC, APC is phosphorylated in vitro by CKIdelta and CKIepsilon
-
-
?
ATP + beta-catenin
ADP + phosphorylated beta-catenin
show the reaction diagram
-
phosphorylation at Ser45, priming GSK3beta phosphorylation
-
-
?
ATP + protein Cubitus interruptus
ADP + phosphorylated protein Cubitus interruptus
show the reaction diagram
-
Ci proteins are important in regulation of Hedgehog signaling, overview
-
-
?
ATP + protein LRP6
ADP + phosphorylated protein LRP6
show the reaction diagram
-
CKIgamma
-
-
?
ATP + protein TCF3
ADP + phosphorylated protein TCF3
show the reaction diagram
-
CKIepsilon
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
P49762
enzyme is essential for eye and embryonic development
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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PKN possesses an autoinhibitory site at the C-terminal C2 domain, which also binds activating arachidonic acid
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
arachidonic acid
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activates in vitro, binds at the autoinhibitory site at the C-terminal C2-domain
Rac1
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GTPase, binds and activates PKN
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Rac2
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GTPase, binds and activates PKN
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Rho1
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small GTPase, binds GTP-dependently and activates PKN
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RhoA
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small GTPase, binds to the ACC domain of PKNalpha forming a catalytic active site
additional information
-
phosphorylation of PKN is required for full activation
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
localized to the nuclei in a speckled pattern. Dmnk proteins become detectable in both somatic and germ line cell nuclei upon their arrival at the periplasm of the syncytial embryo, but then disappear from the somatic cell nuclei. Consistent with mRNA expression, Dmnk proteins in pole cell nuclei are sustained during gastrulation
Manually annotated by BRENDA team
PDB
SCOP
CATH
UNIPROT
ORGANISM
Drosophila melanogaster;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24700
-
alphaxbetax, x * 39833 + x * 24700, calculation from nucleotide sequence
39833
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alphaxbetax, x * 39833 + x * 24700, calculation from nucleotide sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
alphaxbetax, x * 39833 + x * 24700, calculation from nucleotide sequence
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha-subunit and beta-subunit
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overexpression of CKI in Drosophila Kc cell line, the sole Drosophila CKIdelta/epsilon gene, but not CKIalpha, activates a wingless transcriptional reporter in the absence of ligand
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D173N
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the dominant-negative form of CKIgamma inhibits Wnt signaling in a reporter assay in 293T cells, and gish RNAi partially blocks transcription induced by Wg in SL2 cells
K73R
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the dominant-negative form of CKIgamma inhibits Wnt signaling in a reporter assay in 293T cells, and gish RNAi partially blocks transcription induced by Wg in SL2 cells
additional information
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dbtS and dbtL mutations, which alter period length of Drosophila circadian rhythms, produce single amino acid changes in conserved regions of the predicted kinase