Information on EC 2.7.11.1 - non-specific serine/threonine protein kinase and Organism(s) Bos taurus

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The taxonomic range for the selected organisms is: Bos taurus

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.11.1
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RECOMMENDED NAME
GeneOntology No.
non-specific serine/threonine protein kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a protein = ADP + a phosphoprotein
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (non-specific)
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
CAS REGISTRY NUMBER
COMMENTARY hide
191808-15-8
phosphoinositide dependent protein kinase 1
340830-03-7
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37278-10-7
-
377752-08-4
ribosomal protein S6 kinase 2
389133-24-8
ribosomal S6 kinase 3
52660-18-1
casein kinase, protein kinase CK2
9026-43-1
this CAS Reg. No. encompasses a great variety of protein kinases including the serine/threonine specific kinases
90698-26-3
ribosomal protein S6 kinase 1
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + Ac-LRRASLG
ADP + Ac-LRRApSLG
show the reaction diagram
-
an efficient fluorescence assay for serine/threonine kinases using a peptide array is developed. Assay is based on chemical reactions specific to phosphoserine and phosphothreonine residues, that is, base-mediated beta-elimination of the phosphate group and subsequent Michael addition of a thiol-containing fluorescent reagent. This procedure enables the covalent introduction of a fluorescent moiety into the phosphorylated peptide. Novel fluorescent reagents are designed for this purpose and synthesized. As a model peptide, a well-known PKA consensus sequence Kemptide 49 is chosen
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-
?
ATP + bovine serum albumin
?
show the reaction diagram
ATP + casein
?
show the reaction diagram
ATP + eukaryotic protein synthesis initiation factor
?
show the reaction diagram
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i.e. eIF-4E, 1 mol phosphate per mol eIF-4E
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-
?
ATP + glycogen synthase
?
show the reaction diagram
ATP + histone H1
?
show the reaction diagram
ATP + histone IIB
?
show the reaction diagram
ATP + protamine
ADP + O-phosphoprotamine
show the reaction diagram
ATP + several 40S ribosomal polypeptides
?
show the reaction diagram
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e.g. S6, 2.5 mol phosphate per mol S6
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-
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ATP + synthetic peptide
?
show the reaction diagram
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Arg-Arg-Leu-Ser-Ser-Leu-Arg-Ala
-
-
?
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
casein + ATP
phosphorylated casein + ADP
show the reaction diagram
-
-
-
?
RRRDDDSDDD + ATP
? + ADP
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
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substrate of GSK3beta in brain
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-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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can replace Mg2+ with 20% efficiency
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Catalytic subunit of protein phosphatase 2A
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O-phosphoprotamine
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bovine serum albumin, not protamine as substrate
Protein phosphatase 1b
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weak
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Protein phosphatase 2A
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spermine
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bovine serum albumin, not protamine as substrate
staurosporine
-
-
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3',5'-AMP
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i.e. cAMP, activation; not
arachidonic acid
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activates in vitro, binds at the autoinhibitory site at the C-terminus
protein 14-3-3
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a dimeric scaffold protein, protein is absolutely required for connection by simultaneous binding of glycogen synthase kinase-3 beta to tau within a brain microtubule-associated tau phosphorylation complex
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RhoA
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small GTPase, binds to the ACC domain and activates isozymes PKNalpha and PRK2/PKNgamma/PAK-2
additional information
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00025
staurosporine
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
staurosporine
Bos taurus;
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 5
substrate: RRRDDDSDDD
5.11
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7.53
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17.38
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7 - 9
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broad, protamine as substrate
6 - 9
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6.1 - 8.2
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protamine as substrate
6.7
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bovine serum albumin as substrate
7 - 7.6
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eIF-4E as substrate
7.4
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assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 8.8
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about 85% of maximal activity at pH 5.8 and about 80% of maximal activity at pH 8.8, protamine as substrate
6.6 - 6.8
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about half-maximal activity at pH 6.6 and pH 6.8, bovine serum albumin as substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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isozymes show different tissue distribution
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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microsomal kinase is a form of the cytosolic kinase
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Manually annotated by BRENDA team
PDB
SCOP
CATH
UNIPROT
ORGANISM
Bos taurus;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 45000, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
diluted solutions are unstable
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repeated freeze-thawing inactivates
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Triton X-100 stabilizes during freeze-thawing procedures
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, at least 6 months
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-70°C, in 0.025 M Tris-HCl, pH 7.3, 10% glycerol, 1 mM EDTA, 1 mM benzamidine, 0.1 mM PMSF, 14 mM 2-mercaptoethanol, 0.1% Triton X-100, 12 months
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0-4°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, up to 3 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cytosolic
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isozyme PKNalpha from brain membrane
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microsomal
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mitochondrial kinase
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
co-expression of glycogen synthase kinase-3 beta, tau, and 14-3-3 in COS-7 cells and in HEK-293 cells
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