Information on EC 2.7.1.15 - ribokinase and Organism(s) Homo sapiens

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This record set is specific for:
Homo sapiens


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria


The taxonomic range for the selected organisms is: Homo sapiens

EC NUMBER
COMMENTARY hide
2.7.1.15
-
RECOMMENDED NAME
GeneOntology No.
ribokinase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ribose phosphorylation
-
-
degradation of pentoses
-
-
Pentose phosphate pathway
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-ribose 5-phosphotransferase
2-Deoxy-D-ribose can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-84-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-arabinose
?
show the reaction diagram
-
weak activity
-
-
?
ATP + D-arabinose
ADP + D-arabinose 5-phosphate
show the reaction diagram
-
4% if the activity with D-ribose
-
-
?
ATP + D-ribose
ADP + D-ribose 5-phosphate
show the reaction diagram
additional information
?
-
-
no significant phosphorylation of D-fructose, D-galactose and D-xylose
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
may partly support activity
Co2+
about 60% of the activity with Mn2+
Mg2+
about 70% of the activity with Mn2+
Mn2+
best activator, optimum concentration 0.1 mM
Ni2+
may partly support activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-carboxyethylphosphonic acid
-
-
clodronate
-
-
D-ribose
-
substrate inhibition
EDTA
complete inhibition
etidronate
-
-
N-(phosphonomethyl)-glycine
-
-
N-(phosphonomethyl)iminodiacetic acid
-
-
Phosphonoacetic acid
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphate
-
increases catalytic activity. KM-for ribose and ATP decreases in a concentration-dependent manner; marked dependency upon the presence of inorganic phosphate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029 - 7.48
ATP
0.08 - 0.12
D-ribose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 9.8
ATP
0.01 - 10.4
D-ribose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0013 - 250
ATP
0.08 - 90
D-ribose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 18.91
ATP
additional information
additional information
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-
-
PDB
SCOP
CATH
UNIPROT
ORGANISM
Homo sapiens;
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E202L
mutation in NXXE motif, increase in the Km for MgATP of 52fold, and a decrease in the kcat value of around 200fold
N199L
mutation in NXXE motif, increase in the Km for MgATP- by about 68fold, a decrease in the kcat between 573- and 1036fold and almost no change in the Km for D-ribose. No substrate inhibition by D-ribose