Information on EC 2.7.1.137 - phosphatidylinositol 3-kinase and Organism(s) Bos taurus

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Bos taurus


The taxonomic range for the selected organisms is: Bos taurus

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.137
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RECOMMENDED NAME
GeneOntology No.
phosphatidylinositol 3-kinase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-phosphoinositide biosynthesis
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Inositol phosphate metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase
One mammalian isoform is known.
CAS REGISTRY NUMBER
COMMENTARY hide
115926-52-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1-O-octadecyl-2-O-methyl-rac-3-glycerophospho-myo-inositol
ADP + ?
show the reaction diagram
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?
ATP + 1-phosphatidylinositol
ADP + phosphatidylinositol 3-phosphate
show the reaction diagram
ATP + phosphatidylinositol 4,5-bisphosphate
ADP + phosphatidylinositol 3,4,5-trisphosphate
show the reaction diagram
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weak activity
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?
ATP + phosphatidylinositol 4-phosphate
ADP + phosphatidylinositol 3,4-bisphosphate
show the reaction diagram
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weak activity
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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CaCl2 stimulates enzyme forms PI3KI and PI3KII in absence of MnCl2. Half-maximal activation of PI3KI at 0.1 mM Ca2+, maximal activity at 1 mM, in presence of 10 mM MgCl2 further 2fold activation by 2 mM Ca2+. For PI3KII maximal activity is obtained in presence of 2 mM Ca2+, 1.6fold higher than that obtained in presence of 10 mM MgCl2
Mn2+
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scarcely activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+/-)-2-[hydroxy[tetrahydro-2-(octadecyloxy)methylfuran-2-yl]methoxyl]phosphinyloxy-N,N,N-trimethylethaniminium hydroxide
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1-O-octadecyl-2-O-methyl-rac-3-glycerophospho-myo-inositol
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1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine
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noncompetitive with ATP
5'-p-fluorosulfonylbenzoyladenosine
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adenosine
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above 0.1 mM
ADP
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above 0.1 mM
AMP
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above 0.1 mM
Ca2+
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0.1 mM, 60% inhibition, in presence of 10 mM MgCl2
cardiolipin
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Dodecyl sucrose
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hexadexylphosphocholine
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isoquercetin
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PI 3-kinase I and PI 3-kinase II; strong inhibition of PI 3-kinase I and II, noncompetitive, apparent Ki value: 4 mM for PI 3-kinase I and 2.5 mM for PI 3-kinase II
LY301497
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Mg2+
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inhibition above 2.5 mM, activation below
Nonidet P40
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octylglucoside
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phosphatidylcholine
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strong inhibition of enzyme form PI3KII, weak inhibition of enzyme form PI3KI
quercetin
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inhibition of PI 3-kinase I and II; PI 3-kinase I and PI 3-kinase II, non-competitive
sodium cholate
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Sodium deoxycholate
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Triton X-100
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Wortmannin
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphatidic acid
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enhances activity more markedly for PI 3-kinase II than for PI 3-kinase I
additional information
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insulin and IGF-I treatment increases phosphatidylinositol 3-kinase activity 4.5fold in aPY20 immunoprecipitation from whole cell lysates
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.067
ATP
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0.034
phosphatidylinositol
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sonicated
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0017
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0.05
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dimeric enzyme form PI3KII
0.25
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monomeric enzyme form PI3KI
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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enzyme forms PI3KII and PI3KI
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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activation of PI3K signaling is required for the inhibitory effect of Axl on mineral deposition
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Bos taurus;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
124000
1 * 85000 + 1 * 124000, deduced from nucleotide sequence
190000
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heterodimeric form PI3KII, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 85000, SDS-PAGE
monomer
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1 * 110000, enzyme form PI3KI, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
large scale
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PI3KII, heterodimeric form; PI3KI, monomeric form
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type I phosphatidylinositol kinase
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of the 110000 Da subunit in Sf9 insect cells and in COS-1 cells; p110 expressed in insect cells possesses Pl3-kinase activity and associates with p85 alpha into an active p85 alpha-p110 complex that binds the activated colony-stimulating factor 1 receptor. p110 expressed in COS-1 cells is catalytically active only when complexed with p85 alpha
expression of the p85 subunit and a related p85beta protein by using baculovirus expression system
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