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Information on EC 2.7.1.130 - tetraacyldisaccharide 4'-kinase and Organism(s) Pseudomonas aeruginosa

for references in articles please use BRENDA:EC2.7.1.130

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IUBMB Comments

Involved with EC 2.3.1.129 (acyl-[acyl-carrier-protein]—UDP-N-acetylglucosamine O-acyltransferase) and EC 2.4.1.182 (lipid-A-disaccharide synthase) in the biosynthesis of the phosphorylated glycolipid, lipid A, in the outer membrane of Gram-negative bacteria.

The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

Synonyms
lipid a 4'-kinase, tetraacyldisaccharide 4'-kinase, membrane-bound tetraacyldisaccharide-1-phosphate 4'-kinase, more

SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP:2,2',3,3'-tetrakis[(3R)-3-hydroxytetradecanoyl]-beta-D-glucosaminyl-(1->6)-alpha-D-glucosaminyl-phosphate 4'-O-phosphotransferase
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-
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kinase, lipid A 4'-(phosphorylating)
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-
-
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lipid A 4'-kinase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
lipid IVA biosynthesis (2,3-diamino-2,3-dideoxy-D-glucopyranose-containing), lipid IVA biosynthesis (E. coli), lipid IVA biosynthesis (generic), lipid IVA biosynthesis (H. pylori), lipid IVA biosynthesis (P. gingivalis), lipid IVA biosynthesis (P. putida), lipid IVA biosynthesis (Vibrio cholerae serogroup O1 El Tor)
SYSTEMATIC NAME
IUBMB Comments
ATP:2-deoxy-2-([(3R)-3-hydroxyacyl]amino)-3-O-[(3R)-3-hydroxyacyl]-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxyacyl]-2-([(3R)-3-hydroxyacyl]amino)-1-O-phospho-alpha-D-glucopyranose 4'-O-phosphotransferase
Involved with EC 2.3.1.129 (acyl-[acyl-carrier-protein]---UDP-N-acetylglucosamine O-acyltransferase) and EC 2.4.1.182 (lipid-A-disaccharide synthase) in the biosynthesis of the phosphorylated glycolipid, lipid A, in the outer membrane of Gram-negative bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
107309-06-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY hide
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl phosphate)
ADP + (2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl phosphate)
show the reaction diagram
Substrates: -
Products: -
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY hide
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl phosphate)
ADP + (2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl phosphate)
show the reaction diagram
Substrates: -
Products: -
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-amino-1-(3,4-dimethoxyphenyl)-1H-1,2,3-triazole-4-carboxamide
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5-amino-1-(3-chloro-4-methoxyphenyl)-1H-1,2,3-triazole-4-carboxamide
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5-amino-1-(3-chlorophenyl)-1H-1,2,3-triazole-4-carboxamide
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5-amino-1-(4-bromophenyl)-1H-1,2,3-triazole-4-carboxamide
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5-amino-1-(4-ethoxyphenyl)-1H-1,2,3-triazole-4-carboxamide
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additional information
comparative metabolic pathway analysis (Metacyc), data mining from a database of essential genes (DEG), homology modeling, molecular docking, pharmacophore based virtual screening, ADMET prediction and molecular dynamics simulation are used to identify compounds as potential enzyme inhibitor drugs
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top print hide Go to Organism Search
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line Links Gene Links
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
tetraacyldisaccharide 4'-kinase (LpxK) of Pseudomonas aeruginosa is an important tparticipant in the biosynthesis of lipopolysaccharide
additional information
the enzyme's active site is modeled with residues Tyr28, Val31, Arg35, Asn58, Val61, Gly62, Thr64, Lys66, Ser88, Arg89, Gly90, Tyr91, Glu114, Pro115, Arg72, Arg132, Asp151, Asp152, Gln155, His156, Leu180, Arg186 and Glu187
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A8G7EPJ3_PSEAI
332
0
36736
TrEMBL
-
A0A8G7PW11_PSEAI
332
0
36732
TrEMBL
-
A0A6B1Y9H5_PSEAI
332
0
36731
TrEMBL
-
A0A8G6VJ19_PSEAI
332
0
36758
TrEMBL
-
A0A8F9PPA8_PSEAI
332
0
36745
TrEMBL
-
A0A8G3Z0N6_PSEAI
332
0
36774
TrEMBL
-
A0A8G4K1Z3_PSEAI
332
0
36736
TrEMBL
-
A0A8G5RCA6_PSEAI
332
0
36727
TrEMBL
-
A0A0A8RCZ1_PSEAI
332
0
36730
TrEMBL
-
A0A071L1K6_PSEAI
66
2
7492
TrEMBL
-
A0A8G2KU46_PSEAI
332
0
36718
TrEMBL
-
A0A8G6T5M3_PSEAI
332
0
36677
TrEMBL
-
A0A8G7A9F2_PSEAI
332
0
36657
TrEMBL
-
A0A8G4E8E3_PSEAI
332
0
36762
TrEMBL
-
A0A072ZSZ2_PSEAI
332
0
36746
TrEMBL
-
A0A6H3G9T1_PSEAI
332
0
36776
TrEMBL
-
A0A643IYY4_PSEAI
227
0
25071
TrEMBL
-
A0A3S0LX04_PSEAI
332
0
36859
TrEMBL
-
A0A8G7DK14_PSEAI
332
0
36736
TrEMBL
-
A0A659BJR8_PSEAI
332
0
36745
TrEMBL
-
A0A0C6EVS4_PSEAI
332
0
36745
TrEMBL
-
A0A8G4I913_PSEAI
332
0
36673
TrEMBL
-
A0A8F9JM03_PSEAI
332
0
36788
TrEMBL
-
A0A8A9A4C3_PSEAI
332
0
36674
TrEMBL
-
A0A8G4D483_PSEAI
332
0
36774
TrEMBL
-
A0A8G3JPD6_PSEAI
332
0
36774
TrEMBL
-
A0A8G7AF94_PSEAI
332
0
36756
TrEMBL
-
A0A8G3CGT1_PSEAI
332
0
36732
TrEMBL
-
A0A485EZ09_PSEAI
332
0
36833
TrEMBL
-
A0A643INS5_PSEAI
101
0
11238
TrEMBL
-
A0A8F9KF27_PSEAI
332
0
36762
TrEMBL
-
A0A2R3IVI4_PSEAI
332
0
36831
TrEMBL
-
A0A2R3ITI0_PSEAI
154
2
17242
TrEMBL
-
A0A8G7HMF0_PSEAI
332
0
36759
TrEMBL
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
several pharmacoinformatics tools such as comparative metabolic pathway analysis (Metacyc), data mining from a database of essential genes (DEG), homology modeling, molecular docking, pharmacophore based virtual screening, ADMET prediction, and molecular dynamics simulation are used to identify the enzyme as a drug target and identify lead compounds against this target
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Damale, M.; Pathan, S.; Patil, R.; Sangshetti, J.
Pharmacoinformatics approaches to identify potential hits against tetraacyldisaccharide 4'-kinase (LpxK) of Pseudomonas aeruginosa
RSC Adv.
10
32856-32874
2020
Pseudomonas aeruginosa (Q9HZM3), Pseudomonas aeruginosa ATCC 15692 (Q9HZM3), Pseudomonas aeruginosa 1C (Q9HZM3), Pseudomonas aeruginosa PRS 101 (Q9HZM3), Pseudomonas aeruginosa DSM 22644 (Q9HZM3), Pseudomonas aeruginosa CIP 104116 (Q9HZM3), Pseudomonas aeruginosa LMG 12228 (Q9HZM3), Pseudomonas aeruginosa JCM 14847 (Q9HZM3)
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Manually annotated by BRENDA team