Information on EC 2.7.1.108 - dolichol kinase and Organism(s) Homo sapiens

for references in articles please use BRENDA:EC2.7.1.108
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Homo sapiens


The taxonomic range for the selected organisms is: Homo sapiens

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.108
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RECOMMENDED NAME
GeneOntology No.
dolichol kinase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
Phosphorylation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
dolichol and dolichyl phosphate biosynthesis
N-Glycan biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
CTP:dolichol O-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
71768-07-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
dolichol kinase catalyzes the final step in biosynthesis of dolichol phosphate, which is the oligosaccharide carrier required for protein N-glycosylation
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + dolichol
CDP + dolichyl phosphate
show the reaction diagram
additional information
?
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the chain length of eukaryotic dolichol molecules is species specific and differs from 14-17 isoprene units in unicellular organisms like the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, mammalian cells produce longer dolichol molecules with 18-21 isoprene units
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + dolichol
CDP + dolichyl phosphate
show the reaction diagram
additional information
?
-
-
the chain length of eukaryotic dolichol molecules is species specific and differs from 14-17 isoprene units in unicellular organisms like the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, mammalian cells produce longer dolichol molecules with 18-21 isoprene units
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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extracts gained from patient fibroblasts show remarkably lower enzyme activity than extracts from control cells, in comparison with controls, the decrease in activity was 94.5-98.6% for all patients
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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polytopic membrane protein localized in the endoplasmic reticulum with an N-terminus extended into the lumen and a cytoplasmically oriented C-terminus
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000 - 60000
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SDS-PAGE
59270
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predicted from cDNA open reading frame
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene dolk, genotyping and identification of DOLK mutations
human cDNA complements defect in dolichol kinase activity in Saccharomyces cerevisiae sec59-1 mutant, human SEC59 gene also expressed in Sf-9 cells
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overexpression of normal or variant forms of hDK in CHO cells and in yeast sec59-1 cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q483K
naturally occuring homozygous c.1447C>A DOLK mutation involved in enzyme deficiency and a phenotype with anatomic malformations and multi-systemic dysfunction