Information on EC 2.6.1.26 - thyroid-hormone transaminase

for references in articles please use BRENDA:EC2.6.1.26
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:


The expected taxonomic range for this enzyme is: Oryctolagus cuniculus

top print hide
EC NUMBER
COMMENTARY hide
2.6.1.26
-
top print hide
RECOMMENDED NAME
GeneOntology No.
thyroid-hormone transaminase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-3,5,3'-triiodothyronine + 2-oxoglutarate = 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
L-3,5,3'-triiodothyronine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Acts on monoiodotyrosine, diiodotyrosine, triiodothyronine, thyroxine and dinitrotyrosine (unlike EC 2.6.1.24 diiodotyrosine transaminase, which does not act on dinitrotyrosine). Pyruvate or oxaloacetate can act as acceptors.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
51004-29-6
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,5-dibromo-L-tyrosine + 2-oxoglutarate
3-(3,5-dibromo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
3,5-diiodo-D-tyrosine + 2-oxoglutarate
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
very low activity
-
-
r
3,5-diiodo-L-tyrosine + 2-oxoglutarate
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
3,5-dinitro-L-tyrosine + 2-oxoglutarate
3-(3,5-dinitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
i.e. 3,5-dinitro-p-hydroxyphenylpyruvic acid + Glu
r
3-amino-L-tyrosine + 2-oxoglutarate
3-(3-amino-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
very low activity
-
-
r
3-iodo-L-tyrosine + 2-oxoglutarate
3-(3-iodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
3-nitro-L-tyrosine + 2-oxoglutarate
3-(3-nitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
L-3,5,3'-triiodothyronine + 2-oxoglutarate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
r
L-3,5,3'-triiodothyronine + oxaloacetate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine
show the reaction diagram
-
-
i.e. 3,5,3'-triiodothyrophenylpyruvate + Gly
r
L-3,5,3'-triiodothyronine + pyruvate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-alanine
show the reaction diagram
-
-
i.e. 3,5,3'-triiodothyrophenylpyruvate + L-Ala
r
L-phenylalanine + 2-oxoglutarate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
low activtiy
-
-
r
L-thyroxine + 2-oxoglutarate
3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
L-tryptophan + 2-oxoglutarate
3-indole-2-oxopropanoate + L-glutamate
show the reaction diagram
-
low activtiy
-
-
r
L-tyrosine + 2-oxoglutarate
2-oxo-3-phenylpropanoate + L-glutamate
show the reaction diagram
-
low activtiy
-
-
r
additional information
?
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-3,5,3'-triiodothyronine + 2-oxoglutarate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
r
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
partial requirement
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-hydroxymercuribenzoate
-
complete inhibition at 0.1 mM
top print hide
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
absolutely required
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2 - 2
2-oxoglutarate
0.9
3,5-diiodo-L-tyrosine
-
pH 7.8, 37°C
4.4
3,5-Dinitro-L-tyrosine
-
pH 7.8, 37°C
3.8
3-iodo-L-tyrosine
-
pH 7.8, 37°C
2.12
L-thyronine
-
pH 7.8, 37°C
0.034
L-triiodothyronine
-
pH 7.8, 37°C
2
pyruvate
-
cosubstrate L-triiodothyronine, pH 7.8, 37°C
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1 - 6.6
3,5-diiodo-L-tyrosine
39.9
3,5-Dinitro-L-tyrosine
-
pH 7.8, 37°C
3.68
3-iodo-L-tyrosine
-
pH 7.8, 37°C
0.0233
L-thyroxine
-
pH 7.8, 37°C
0.0317
L-triiodothyronine
-
pH 7.8, 37°C
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.013
-
purified enzyme, substrates L-3,5,3'-triiodothyronine + 2-oxoglutarate
15
-
purified enzyme, substrates 3,5-dinitro-L-tyrosine + 2-oxoglutarate
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 8.6
-
broad optimum
top print hide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.6
-
pH 7.0: 30% of maximal activity with L-triiodothyronine, 15% of maximal activity with 3,5-dinitro-L-tyrosine, pH 7.4-8.6: maximal activity with triiodothyronine and 3,5-dinitro-L-tyrosine
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
low activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
additional information
-
not in serum
Manually annotated by BRENDA team
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
2 * 48000, SDS-PAGE
95000
-
glycerol density gradient centrifugation
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 48000, SDS-PAGE
top print hide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 30% loss of activity per month
-
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 2.6.1.26)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)