We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments A pyridoxal-phosphate protein. Acts on monoiodotyrosine, diiodotyrosine, triiodothyronine, thyroxine and dinitrotyrosine (unlike EC 2.6.1.24 diiodotyrosine transaminase, which does not act on dinitrotyrosine). Pyruvate or oxaloacetate can act as acceptors.
The expected taxonomic range for this enzyme is: Oryctolagus cuniculus
Synonyms
3,5-dinitrotyrosine transaminase, aminotransferase, thyroid hormone,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3,5-dinitrotyrosine transaminase
-
-
-
-
aminotransferase, thyroid hormone
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-3,5,3'-triiodothyronine + 2-oxoglutarate = 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
amino group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-3,5,3'-triiodothyronine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Acts on monoiodotyrosine, diiodotyrosine, triiodothyronine, thyroxine and dinitrotyrosine (unlike EC 2.6.1.24 diiodotyrosine transaminase, which does not act on dinitrotyrosine). Pyruvate or oxaloacetate can act as acceptors.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3,5-dibromo-L-tyrosine + 2-oxoglutarate
3-(3,5-dibromo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
-
r
3,5-diiodo-D-tyrosine + 2-oxoglutarate
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
very low activity
-
-
r
3,5-diiodo-L-tyrosine + 2-oxoglutarate
3-(3,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
-
r
3,5-dinitro-L-tyrosine + 2-oxoglutarate
3-(3,5-dinitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
i.e. 3,5-dinitro-p-hydroxyphenylpyruvic acid + Glu
r
3-amino-L-tyrosine + 2-oxoglutarate
3-(3-amino-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
very low activity
-
-
r
3-iodo-L-tyrosine + 2-oxoglutarate
3-(3-iodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
-
r
3-nitro-L-tyrosine + 2-oxoglutarate
3-(3-nitro-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
-
-
-
r
L-3,5,3'-triiodothyronine + 2-oxoglutarate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
-
-
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
r
L-3,5,3'-triiodothyronine + oxaloacetate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + glycine
-
-
i.e. 3,5,3'-triiodothyrophenylpyruvate + Gly
r
L-3,5,3'-triiodothyronine + pyruvate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-alanine
-
-
i.e. 3,5,3'-triiodothyrophenylpyruvate + L-Ala
r
L-phenylalanine + 2-oxoglutarate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
low activtiy
-
-
r
L-thyronine + 2-oxoglutarate
?
-
-
-
-
?
L-thyroxine + 2-oxoglutarate
3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
-
-
-
-
r
L-tryptophan + 2-oxoglutarate
3-indole-2-oxopropanoate + L-glutamate
-
low activtiy
-
-
r
L-tyrosine + 2-oxoglutarate
2-oxo-3-phenylpropanoate + L-glutamate
-
low activtiy
-
-
r
additional information
?
-
additional information
?
-
-
specific for 2-oxoglutarate
-
-
?
additional information
?
-
-
pyruvate and oxalacetate are ineffective with 3,5-dinitro-L-tyrosine and only partially effective with iodinated tyrosines
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-3,5,3'-triiodothyronine + 2-oxoglutarate
3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-2-oxopropanoate + L-glutamate
-
-
i.e. 3,5,3'-triiodothyropyruvate + L-Glu
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pyridoxal 5'-phosphate
-
partial requirement
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
p-hydroxymercuribenzoate
-
complete inhibition at 0.1 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-mercaptoethanol
-
absolutely required
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.9
3,5-diiodo-L-tyrosine
-
pH 7.8, 37°C
4.4
3,5-Dinitro-L-tyrosine
-
pH 7.8, 37°C
3.8
3-iodo-L-tyrosine
-
pH 7.8, 37°C
2.12
L-thyronine
-
pH 7.8, 37°C
0.034
L-triiodothyronine
-
pH 7.8, 37°C
2
pyruvate
-
cosubstrate L-triiodothyronine, pH 7.8, 37°C
0.2
2-oxoglutarate
-
cosubstrate triiodothyronine, pH 7.8, 37°C
0.2
2-oxoglutarate
-
cosubstrate L-thyroxine, pH 7.8, 37°C
2
2-oxoglutarate
-
cosubstrate 3,5-dinitro-L-tyrosine, pH 7.8, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1.1 - 6.6
3,5-diiodo-L-tyrosine
39.9
3,5-Dinitro-L-tyrosine
-
pH 7.8, 37°C
3.68
3-iodo-L-tyrosine
-
pH 7.8, 37°C
0.0233
L-thyroxine
-
pH 7.8, 37°C
0.0317
L-triiodothyronine
-
pH 7.8, 37°C
1.1
3,5-diiodo-L-tyrosine
-
pH 7.8, 37°C
6.6
3,5-diiodo-L-tyrosine
-
pH 7.8, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.013
-
purified enzyme, substrates L-3,5,3'-triiodothyronine + 2-oxoglutarate
15
-
purified enzyme, substrates 3,5-dinitro-L-tyrosine + 2-oxoglutarate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.4 - 8.6
-
broad optimum
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7 - 8.6
-
pH 7.0: 30% of maximal activity with L-triiodothyronine, 15% of maximal activity with 3,5-dinitro-L-tyrosine, pH 7.4-8.6: maximal activity with triiodothyronine and 3,5-dinitro-L-tyrosine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
low activity
brenda
-
low activity
brenda
-
-
brenda
-
-
brenda
-
low activity
brenda
-
low activity
brenda
additional information
-
not in serum
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
48000
-
2 * 48000, SDS-PAGE
95000
-
glycerol density gradient centrifugation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimer
-
2 * 48000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, 30% loss of activity per month
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Soffer, R.L.; Hechtman, P.; Savage, M.
L-Triiodothyronine aminotransferase
J. Biol. Chem.
248
1224-1230
1973
Oryctolagus cuniculus
brenda
Select items on the left to see more content.
BRENDA home
History
2.6.1.26 thyroid-hormone transaminase
more
top
