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IUBMB Comments The enzyme, characterized from the bacterium Streptomyces davawensis , has the activities of an oxidoreductase, a decarboxylase, and an aminotransferase. Its combined actions result in the replacement of a methyl substituent of one of the aromatic rings of FMN by an amino group, a step in the biosynthetic pathway of roseoflavin. The reaction requires thiamine for completion.
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Synonyms bn159_7989, more
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8-amino-flavin synthase
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8-demethyl-8-aminoriboflavin-5Ā-phosphate synthase
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BN159_7989
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rosB
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8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor = 8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
(1b)
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FMN + O2 = 8-demethyl-8-formylriboflavin 5'-phosphate + H2O
(1a)
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L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + 2 acceptor = 2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + 2 reduced acceptor
(1c)
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L-glutamate + FMN + O2 + H2O + 3 acceptor = 2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + 3 reduced acceptor
overall reaction
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L-glutamate:FMN aminotransferase (oxidizing, decarboxylating)
The enzyme, characterized from the bacterium Streptomyces davawensis, has the activities of an oxidoreductase, a decarboxylase, and an aminotransferase. Its combined actions result in the replacement of a methyl substituent of one of the aromatic rings of FMN by an amino group, a step in the biosynthetic pathway of roseoflavin. The reaction requires thiamine for completion.
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8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
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Substrates: - Products: -
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8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
Substrates: - Products: -
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FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
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Substrates: - Products: -
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FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
Substrates: - Products: -
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L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
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Substrates: - Products: -
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L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
Substrates: - Products: -
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L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
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Substrates: - Products: -
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L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
Substrates: - Products: -
?
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8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
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Substrates: - Products: -
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8-demethyl-8-formylriboflavin 5'-phosphate + H2O + acceptor
8-carboxy-8-demethylriboflavin 5'-phosphate + reduced acceptor
Substrates: - Products: -
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FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
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Substrates: - Products: -
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FMN + O2
8-demethyl-8-formylriboflavin 5'-phosphate + H2O
Substrates: - Products: -
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L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
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Substrates: - Products: -
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L-glutamate + 8-carboxy-8-demethylriboflavin 5'-phosphate + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
Substrates: - Products: -
?
L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
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Substrates: - Products: -
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L-glutamate + FMN + O2 + H2O + acceptor
2-oxoglutarate + 8-amino-8-demethylriboflavin 5'-phosphate + CO2 + reduced acceptor
Substrates: - Products: -
?
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brenda
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UniProt
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Highest Expressing Human Cell Lines
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ROSB_STRDJ
Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)
257
0
28865
Swiss-Prot
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A0A822IRA1_9EURY
277
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31441
TrEMBL
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A0A653J5A2_9SPHN
202
0
21543
TrEMBL
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homotetramer
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4 * 29000, SDS-PAGE
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Ni-NTA column chromatography
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21(DE3) cells
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Schwarz, J.; Konjik, V.; Jankowitsch, F.; Sandhoff, R.; Mack, M.
Identification of the key enzyme of roseoflavin biosynthesis
Angew. Chem. Int. Ed. Engl.
55
6103-6106
2016
Streptomyces davaonensis
brenda
Konjik, V.; Bruenle, S.; Demmer, U.; Vanselow, A.; Sandhoff, R.; Ermler, U.; Mack, M.
The crystal structure of RosB insights into the reaction mechanism of the first member of a family of flavodoxin-like enzymes
Angew. Chem. Int. Ed. Engl.
56
1146-1151
2017
Streptomyces davaonensis
brenda
Jhulki, I.; Chanani, P.; Abdelwahed, S.; Begley, T.
A remarkable oxidative cascade that replaces the riboflavin C8 methyl with an amino group during roseoflavin biosynthesis
J. Am. Chem. Soc.
138
8324-8327
2016
Streptomyces davaonensis (K4REZ6)
brenda
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