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Information on EC 2.5.1.113 - [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase for references in articles please use BRENDA:EC2.5.1.113
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EC Tree
IUBMB Comments A pyridoxal-phosphate protein. The enzyme participates in an alternative pathway for L-cysteine biosynthesis that involves a protein-bound thiocarboxylate as the sulfide donor. The enzyme from the bacterium Mycobacterium tuberculosis also has very low activity with O3-acetyl-L-serine (cf. EC 2.5.1.65, O-phosphoserine sulfhydrylase).
Word Map
2.5.1.113
tubulin
microtubule
polyglutamylation
glutamylation
ligase-like
deglutamylated
cilia
purkinje
carboxypeptidase
The enzyme appears in viruses and cellular organisms
Reaction Schemes
+
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
=
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine
+
Synonyms
CysM, Cysteine synthase, O-phosphoserine sulfhydrylase, O-phosphoserine-specific cysteine synthase,
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O-phosphoserine sulfhydrylase
O-phosphoserine-specific cysteine synthase
CysM
-
-
ambiguous
-
O-phosphoserine sulfhydrylase
-
O-phosphoserine sulfhydrylase
-
-
O-phosphoserine-specific cysteine synthase
-
O-phosphoserine-specific cysteine synthase
-
-
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O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH = [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
-
-
-
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
O-phospho-L-serine:thiocarboxylated [CysO sulfur-carrier protein] 2-amino-2-carboxyethyltransferase
A pyridoxal-phosphate protein. The enzyme participates in an alternative pathway for L-cysteine biosynthesis that involves a protein-bound thiocarboxylate as the sulfide donor. The enzyme from the bacterium Mycobacterium tuberculosis also has very low activity with O3-acetyl-L-serine (cf. EC 2.5.1.65, O-phosphoserine sulfhydrylase).
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O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
?
-
the specificity of the enzyme for its amino acid substrate is more than 500fold greater for O-phospho-L-serine than for O-acetyl-L-serine
-
-
?
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
additional information
?
-
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
-
-
-
-
?
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
-
-
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
-
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
the specificity of the enzyme for the physiological sulfide equivalent [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH is more than 3 orders of magnitude greater than that for bisulfide
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
-
-
-
?
additional information
?
-
-
no activity with O-acetyl-L-serine
-
-
-
additional information
?
-
no activity with O-acetyl-L-serine
-
-
-
additional information
?
-
no activity with O-acetyl-L-serine
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
-
-
-
-
?
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate
-
-
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
-
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
the specificity of the enzyme for the physiological sulfide equivalent [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH is more than 3 orders of magnitude greater than that for bisulfide
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
-
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
dependent on
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0.1
O-phospho-L-serine
-
at pH 7.5 and 37°C
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1.34 - 8.85
O-phospho-L-serine
0.857 - 146
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
1.34
O-phospho-L-serine
-
wild type enzyme, at pH 7.5 and 37°C
8.85
O-phospho-L-serine
-
deletion mutant DELTA319-323, at pH 7.5 and 37°C
0.857
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
deletion mutant DELTA319-323, at pH 7.5 and 37°C
-
146
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
wild type enzyme, at pH 7.5 and 37°C
-
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-
-
-
brenda
-
UniProt
brenda
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UniProt
brenda
-
-
-
brenda
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CYSM_MYCTO
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
323
0
34438
Swiss-Prot
CYSM_MYCTU
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
323
0
34438
Swiss-Prot
CYSM_MYCBO
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
323
0
34438
Swiss-Prot
A0A498Q6Q9_9MYCO
323
0
34459
TrEMBL
A0A0J6VCP9_9MYCO
320
0
34355
TrEMBL
A0A2H5WCM0_9BACT
310
0
33681
TrEMBL
A0A2H5ZE03_9BACT
315
0
33692
TrEMBL
A0A2H5ZFD3_9BACT
314
0
34082
TrEMBL
A0A3N6FKS8_9ACTN
316
0
33755
TrEMBL
A0A3N6FA50_9ACTN
316
0
33692
TrEMBL
A0A1Q2ZNP0_9ACTN
316
0
33792
TrEMBL
K5BHG9_MYCHD
Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 / 3849)
323
0
35003
TrEMBL
A0A2Z5K0L0_9ACTN
316
0
33832
TrEMBL
A0A1K2FT08_9ACTN
316
0
33835
TrEMBL
A0A231H1J0_9NOCA
320
0
34693
TrEMBL
A0A4R8RXY1_9MYCO
320
0
34267
TrEMBL
A0A0J6W1J0_9MYCO
319
0
34237
TrEMBL
A0A2P8AT86_9ACTN
321
0
34491
TrEMBL
A0A1D2IKP5_9ACTN
316
0
33824
TrEMBL
A0A3G4VSX7_9ACTN
316
0
33844
TrEMBL
A0A1S1NJR7_9MYCO
320
0
34380
TrEMBL
A0A1A9GK65_9ACTN
315
0
34048
TrEMBL
A0A1Y2MMU1_PSEAH
331
0
35306
TrEMBL
A0A2S6X0Z0_9ACTN
316
0
33808
TrEMBL
A0A498Q734_9MYCO
323
0
34417
TrEMBL
A0A178WT14_9PSEU
316
0
33970
TrEMBL
A0A117DWG6_9MYCO
314
0
33914
TrEMBL
A0A2H6K499_9BACT
310
0
33967
TrEMBL
A0A1X0L7F6_9MYCO
323
0
34356
TrEMBL
A0A100JQ80_STRSC
316
0
33755
TrEMBL
A0A2P7ZK20_9ACTN
316
0
33866
TrEMBL
A0A2H5YFK4_9BACT
321
0
35415
TrEMBL
A0A3G8JHA7_9ACTN
328
0
35538
TrEMBL
A0A3N6H8H4_9ACTN
316
0
33892
TrEMBL
A0A177HWT5_9ACTN
316
0
33837
TrEMBL
A0A221VZ81_9PSEU
316
0
33642
TrEMBL
A0A385ZPS6_9ACTN
316
0
33778
TrEMBL
A0A3E2YTV2_9ACTN
321
0
34567
TrEMBL
A0A2I2KR51_9ACTN
315
0
33922
TrEMBL
A0A4P6Q2B2_9ACTN
315
0
34221
TrEMBL
A0A4R8STD9_9MYCO
320
0
34260
TrEMBL
A0A2P2C3D7_9ZZZZ
316
0
34125
TrEMBL
A0A0J6WRX6_MYCCU
320
0
34355
TrEMBL
A0A433DZY1_9ACTN
316
0
33803
TrEMBL
A0A387HP15_9ACTN
316
0
33705
TrEMBL
A0A3N6IX21_9ACTN
316
0
33755
TrEMBL
A0A222THQ1_GORRU
323
0
35109
TrEMBL
A0A3N6GD87_9ACTN
316
0
33786
TrEMBL
A0A2H5YCG5_9BACT
329
0
36000
TrEMBL
A0A3N6FTA5_9ACTN
316
0
33785
TrEMBL
A0A399HGY6_9ACTN
316
0
33789
TrEMBL
A0A0L0KL67_9ACTN
316
0
33764
TrEMBL
A0A1B9EPR4_9ACTN
316
0
33861
TrEMBL
A0A3E2MSK5_MYCMR
314
0
33866
TrEMBL
A0A5B7UTL1_9ACTN
316
0
33993
TrEMBL
A0A1Y2NBP0_STRPT
316
0
33737
TrEMBL
A0A2K8PIE4_STRLA
316
0
33741
TrEMBL
A0A1Y2P1X0_STRFR
316
0
33923
TrEMBL
A0A4R8SB59_9MYCO
324
0
34640
TrEMBL
A0A2P2C2W9_9ZZZZ
315
0
33943
TrEMBL
A0A498QVC4_9MYCO
311
0
33364
TrEMBL
A0A4V6QEU7_9MYCO
324
0
34699
TrEMBL
A0A2K8R9H7_9ACTN
316
0
33797
TrEMBL
A0A0K2AST4_STRA7
Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516)
316
0
33883
TrEMBL
A0A2P4UJA8_9ACTN
315
0
33920
TrEMBL
A0A3N6EXV9_9ACTN
316
0
33705
TrEMBL
A0A2X3KYU7_9ACTN
315
0
33936
TrEMBL
A0A4V1AXE3_9PSEU
316
0
33774
TrEMBL
A0A1D8G131_9ACTN
316
0
33923
TrEMBL
A0A3N6DTP8_9ACTN
316
0
33844
TrEMBL
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Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
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homodimer
-
x-ray crystallography
homodimer
-
x-ray crystallography; x-ray crystallography
-
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native enzyme bound to CysO sulfur-carrier protein, hanging drop vapor diffusion method, using 7-10% PEG (w/v) 4000, 0.1 M sodium citrate, pH 5.8, and 0.2 M ammonium acetate at 22°C
-
sitting drop vapor diffusion method, using 0.1 M Tris-HCl, pH 7.25-7.5, 0.1 M K2HPO4, 4.3 M NaCl
-
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DELTA319-323
-
the deletion mutant shows increased catalytic efficiency for O-phospho-L-serine and reduced catalytic efficiency for [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
R220A
-
the mutant shows reduced activity with O-phospho-L-serine and increased activity with O-acetyl-L-serine compared to the wild type enzyme
DELTA319-323
-
the deletion mutant shows increased catalytic efficiency for O-phospho-L-serine and reduced catalytic efficiency for [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
R220A
-
the mutant shows reduced activity with O-phospho-L-serine and increased activity with O-acetyl-L-serine compared to the wild type enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ni-NTA affinity column chromatography and Sephadex 200 gel filtration
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography, Superdex-200 gel filtration, and Sephadex G-25 gel filtration
-
nickel affinity column chromatography
-
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expressed in Escherichia coli B834(DE3) and BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
-
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OLeary, S.E.; Jurgenson, C.T.; Ealick, S.E.; Begley, T.P.
O-Phospho-l-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis
Biochemistry
47
11606-11615
2008
Mycobacterium tuberculosis
brenda
Agren, D.; Schnell, R.; Oehlmann, W.; Singh, M.; Schneider, G.
Cysteine synthase (CYSM) of Mycobacterium tuberculosis is an O-phosphoserine sulfhydrylase: Evidence for an alternative cysteine biosynthesis pathway in mycobacteria
J. Biol. Chem.
283
31567-31574
2008
Mycobacterium tuberculosis, Mycobacterium tuberculosis (P9WP53), Mycobacterium tuberculosis H37Rv (P9WP53)
brenda
Jurgenson, C.T.; Burns, K.E.; Begley, T.P.; Ealick, S.E.
Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of Mycobacterium tuberculosis
Biochemistry
47
10354-10364
2008
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Agren, D.; Schnell, R.; Schneider, G.
The C-terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity
FEBS Lett.
583
330-336
2009
Mycobacterium tuberculosis (P9WP53), Mycobacterium tuberculosis H37Rv (P9WP53)
brenda
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2.5.1.113 [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase
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