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EC Tree
IUBMB Comments 7-O-beta-D-Glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors.
The expected taxonomic range for this enzyme is: Petroselinum crispum
Synonyms
apiosyltransferase, uridine diphosphoapiose-flavone, UDPapiose:7-O-(beta-D-glucosyl)-flavone apiosyltransferase,
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apiosyltransferase, uridine diphosphoapiose-flavone
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UDPapiose:7-O-(beta-D-glucosyl)-flavone apiosyltransferase
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UDP-alpha-D-apiose + apigenin 7-O-beta-D-glucoside = UDP + apigenin 7-O-[beta-D-apiosyl-(1->2)-beta-D-glucoside]
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pentosyl group transfer
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UDP-apiose:5,4'-dihydroxyflavone 7-O-beta-D-glucoside 2''-O-beta-D-apiofuranosyltransferase
7-O-beta-D-Glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors.
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UDPapiose + 7-O-(beta-D-glucosyl)-apigenin
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biosynthesis of apiin
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UDPapiose + 7-O-(beta-D-glucosyl)-apigenin
UDP + 7-O-beta-D-apiofuranosyl-1,2-beta-D-glucosyl-apigenin
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specific for UDPapiose as glycosyl donor
i.e. apiin
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UDPapiose + biochanin A
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i.e. 5,7-dihydroxy-4'-methoxyisoflavone-7-glucoside
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additional information
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overview: 7-O-beta-glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors, not: flavonol-3-glucoside, flavonol-7-glucoside, apigenin-8-C-glucoside, aglycones of flavonoids, glucose
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UDPapiose + 7-O-(beta-D-glucosyl)-apigenin
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biosynthesis of apiin
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Chrysoeriol-7-glucoside
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iodoacetamide
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at 1 mM causes an inhibition of 55%, reversed by cysteine
p-chloromercuribenzoate
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at 1 mM inhibits the reaction completely
additional information
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no effect: EDTA, NH4+, K+
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dithioerythritol
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stimulates enzyme activity up to 35%, optimum concentration: 6 mM
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0.066
apigenin-7-glucoside
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additional information
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7
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higher apiin yield in Tris-HCl than in phosphate buffer
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brenda
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brenda
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brenda
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6 - 8
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highest stability
637786
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albumin, 0.1 mg, significant stabilization
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solution of enzyme after hydroxylapatite chromatography in sodium phosphate buffer, pH 7.0, rapidly loses activity upon freezing
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-20°C, Tris-HCl, dithiothreitol, protein concentration 0.5-1 mg/ml
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purified 123fold by protamine sulfate and ammonium sulfate precipitation and chromatography on DEAE-cellulose, SephadexG-100 and hydroxylapatite
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Ortmann, R.; Sutter, A.; Grisebach, H.
Purification and properties of udpapiose: 7-O-(beta-D-glucosyl)-flavone apiosyltransferase from cell suspension cultures of parsley
Biochim. Biophys. Acta
289
293-302
1972
Petroselinum crispum
brenda
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