We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments 7-O-beta-D-Glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors.
The expected taxonomic range for this enzyme is: Petroselinum crispum
Synonyms
apiosyltransferase, uridine diphosphoapiose-flavone, UDPapiose:7-O-(beta-D-glucosyl)-flavone apiosyltransferase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
apiosyltransferase, uridine diphosphoapiose-flavone
-
-
-
-
UDPapiose:7-O-(beta-D-glucosyl)-flavone apiosyltransferase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-apiose + apigenin 7-O-beta-D-glucoside = UDP + apigenin 7-O-[beta-D-apiosyl-(1->2)-beta-D-glucoside]
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pentosyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-apiose:5,4'-dihydroxyflavone 7-O-beta-D-glucoside 2''-O-beta-D-apiofuranosyltransferase
7-O-beta-D-Glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin
?
-
biosynthesis of apiin
-
-
?
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin
UDP + 7-O-beta-D-apiofuranosyl-1,2-beta-D-glucosyl-apigenin
-
specific for UDPapiose as glycosyl donor
i.e. apiin
?
UDPapiose + biochanin A
?
-
i.e. 5,7-dihydroxy-4'-methoxyisoflavone-7-glucoside
-
-
?
additional information
?
-
-
overview: 7-O-beta-glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors, not: flavonol-3-glucoside, flavonol-7-glucoside, apigenin-8-C-glucoside, aglycones of flavonoids, glucose
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin
?
-
biosynthesis of apiin
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Chrysoeriol-7-glucoside
-
-
iodoacetamide
-
at 1 mM causes an inhibition of 55%, reversed by cysteine
p-chloromercuribenzoate
-
at 1 mM inhibits the reaction completely
additional information
-
no effect: EDTA, NH4+, K+
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dithioerythritol
-
stimulates enzyme activity up to 35%, optimum concentration: 6 mM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.066
apigenin-7-glucoside
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7
-
higher apiin yield in Tris-HCl than in phosphate buffer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6 - 8
-
highest stability
637786
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
albumin, 0.1 mg, significant stabilization
-
solution of enzyme after hydroxylapatite chromatography in sodium phosphate buffer, pH 7.0, rapidly loses activity upon freezing
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, Tris-HCl, dithiothreitol, protein concentration 0.5-1 mg/ml
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
purified 123fold by protamine sulfate and ammonium sulfate precipitation and chromatography on DEAE-cellulose, SephadexG-100 and hydroxylapatite
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ortmann, R.; Sutter, A.; Grisebach, H.
Purification and properties of udpapiose: 7-O-(beta-D-glucosyl)-flavone apiosyltransferase from cell suspension cultures of parsley
Biochim. Biophys. Acta
289
293-302
1972
Petroselinum crispum
brenda
Select items on the left to see more content.
html completed
BRENDA home
History
2.4.2.25 flavone apiosyltransferase
more
top
