Information on EC 2.4.1.368 - oleanolate 3-O-glucosyltransferase

Substrates: the enzyme specifically glucosylates oleanoate at the C3 position
Products: -

Zingibroside R1

Ginsenoside Ro +UDP

AKA44603.1, AIE12488.1

Substrates: oleanane-type ginsenoside biosynthesis in P. ginseng callus
Products: -

zingibroside R1 + UDP-Glc

ginsenoside Ro + UDP

AKA44603.1, AIE12488.1

Substrates: high affinity
Products: -

additional information

3 entries

apigenin + UDP-Glc

apigenin-7-glucoside + UDP

Substrates: flavonoid patway
Products: -

apigenin + UDP-Glc

apigenin-7-glucoside + UDP

Substrates: -
Products: -

chikusetsusaponin IVa + UDP-Glc

ginsenoside Ro + UDP

AKA44603.1, AIE12488.1

Substrates: -
Products: -

chikusetsusaponin IVa + UDP-Glc

ginsenoside Ro + UDP

AKA44603.1, AIE12488.1

Substrates: high affinity and catalytic efficiency
Products: -

oleanolic acid +UDP-GlcA

calenduloside E +UDP

AKA44603.1, AIE12488.1

Substrates: the studies revealed that PgCSyGT1 is the key GT in ginseng responsible for calenduloside E biosynthesis via transfer of the glucuronosyl moiety of UDP-glucuronic acid to the C3 position of oleanolic acid
Products: -

oleanolic acid +UDP-GlcA

calenduloside E +UDP

AKA44603.1, AIE12488.1

Substrates: -
Products: -

additional information

Substrates: the enzyme shows weak activity toward quercetin and kaempferol at 0.1 mM concentration, while at 0.01 mM no activity detected
Products: -

additional information

Substrates: the enzyme shows weak activity toward quercetin and kaempferol at 0.1 mM concentration, while at 0.01 mM no activity detected
Products: -

additional information

Substrates: no activity with UDP-glucuronic acid
Products: -

show all columns Go to Natural Substrate Search

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

apigenin + UDP-Glc

apigenin-7-glucoside + UDP

Substrates: flavonoid patway
Products: -

Calenduloside E

Chikusetsusaponin IVa +UDP

AKA44603.1, AIE12488.1

Substrates: oleanane-type ginsenoside biosynthesis in P. ginseng callus
Products: -

calenduloside E + UDP-Glc

zingibroside R1 + UDP

AKA44603.1, AIE12488.1

Substrates: -
Products: -

chikusetsusaponin IVa + UDP-Glc

ginsenoside Ro + UDP

AKA44603.1, AIE12488.1

Substrates: -
Products: -

luteolin

luteolin glucosides

Substrates: flavonoid patway
Products: -

luteolin + UDP-Glc

luteolin-7-glucoside + UDP

Substrates: flavonoid patway
Products: -

oleanolic acid + UDP-Glc

AKA44603.1, AIE12488.1

Substrates: -
Products: -

oleanolic acid +UDP-GlcA

calenduloside E +UDP

AKA44603.1, AIE12488.1

quercetin

quercetin glucosides

Substrates: flavonoid patway
Products: -

quercetin + UDP-Glc

quercetin-7-glucoside + UDP

Substrates: flavonoid patway
Products: -

UDP-alpha-D-glucose + oleanolate

UDP + oleanolate 3-O-beta-D-glucoside

Substrates: the enzyme specifically glucosylates oleanoate at the C3 position
Products: -

Zingibroside R1

Ginsenoside Ro +UDP

AKA44603.1, AIE12488.1

Substrates: oleanane-type ginsenoside biosynthesis in P. ginseng callus
Products: -

show all columns Go to Metals and Ions Search

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Mg2+

about 110% activity at 5 mM

additional information

not influenced by Ba2+, Ni2+, Mn2+, and Ca2+

show all columns Go to Inhibitor Search

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

Co2+

about 68% residual activity at 5 mM

Cu2+

complete inhibition at 5 mM

Fe2+

about 85% residual activity at 5 mM

Zn2+

about 22% residual activity at 5 mM

additional information

not inhibited by EDTA

show all columns Go to KM Value Search

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.097 - 0.691

calenduloside E

0.304

chikusetsusaponin IVa

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.104

glycyrrhetinic acid

at pH 7.4 and 37°C

0.041

luteolin

pH 8.0, Temp 37 °C, 30 min

0.853

oleanolic acid

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.036

quercetin

pH 8.0, Temp 37 °C, 30 min

0.486

UDP-alpha-D-glucose

at pH 7.4 and 37°C

0.28

zingibroside R1

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.097

calenduloside E

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.691

calenduloside E

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

show all columns Go to Turnover Number Search

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.001 - 0.025

calenduloside E

0.002

chikusetsusaponin IVa

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.091

glycyrrhetinic acid

at pH 7.4 and 37°C

1.16

luteolin

pH 8.0, Temp 37 °C, 30 min

0.034

oleanolic acid

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

5.93

quercetin

pH 8.0, Temp 37 °C, 30 min

0.102

UDP-alpha-D-glucose

at pH 7.4 and 37°C

0.054

zingibroside R1

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.001

calenduloside E

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.025

calenduloside E

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

show all columns Go to kcat/KM Value Search

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.001 - 0.257

calenduloside E

0.006

chikusetsusaponin IVa

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.873

glycyrrhetinic acid

at pH 7.4 and 37°C

28.5

luteolin

pH 8.0, Temp 37 °C, 30 min

0.04

oleanolic acid

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

165.69

quercetin

pH 8.0, Temp 37 °C, 30 min

0.215

UDP-alpha-D-glucose

at pH 7.4 and 37°C

0.194

zingibroside R1

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.001

calenduloside E

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

0.257

calenduloside E

AKA44603.1, AIE12488.1

pH 7.0, 30 °C, 12h

show all columns Go to pH Optimum Search

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

show all columns Go to pH Range Search

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6 - 8

more than 60% activity between pH 6.0 and 8.0

show all columns Go to Temperature Optimum Search

AKA44603.1, AIE12488.1

Tris-HCl 50 mM

Tris-HCl 50 mM

TrisâHCl 50 mM

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

show all columns Go to Temperature Range Search

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TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

25 - 45

more than 50% activity between 25 and 45°C

AKA44603.1, AIE12488.1

Incubation temperature for enzymatic activity and kinetic assays for 1-12 h under standard conditions

Incubations carried out for 30 min - 6 h

Incubations carried out for 30 min â 6 h

show all columns Go to Organism Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

brenda

UniProt

brenda

brenda

Barbarea vulgaris subsp. arcuata

UniProt

brenda

brenda

AIE12488.1, AKA44603.1

GenBank

brenda

show all columns Go to Source Tissue Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

AKA44603.1, AIE12488.1

brenda

show all columns Go to Localization Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

show all columns Go to General Information Search

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

metabolism

4 entries

metabolism

the enzyme is involved in saponin biosynthesis

metabolism

EpUGT95 is a key player in facilitating the di-glucosylation processes of luteolin and quercetin

metabolism

EpUGT73 facilitates glucose glycosylation at the C7 position of apigenin, luteolin and quercetin, exhibiting a remarkable substrate heterogeneity and catalytic site specificity

metabolism

AKA44603.1, AIE12488.1

biosynthesis of diverse oleanane-type ginsenosides

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

K4GHS2 pBLAST

73C13_BARVU

495

55427

Swiss-Prot

K4GGT4 pBLAST

73C11_BARVU

495

55613

Swiss-Prot

K4GIP0 pBLAST

73C12_BARVU

495

55576

Swiss-Prot

K4GHR9 pBLAST

UGT1_BARVU

495

55599

Swiss-Prot

K4GKX2 pBLAST

73C10_BARVU

495

55486

Swiss-Prot

show all columns Go to Subunit Search

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

x * 62000, SDS-PAGE

Go to Purification (commentary) Search

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

Ni-NTA column chromatography

purified from the cell extracts using glutathione agarose affinity chromatography

AKA44603.1, AIE12488.1

Go to Cloned (commentary) Search

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

cloned from E. phaseoloides into pMal-C2X vector

expressed in Escherichia coli BL21(DE3) cells

expressed in Escherichia coli XJb(DE3) cells

expressed in Nicotiana benthamiana

expression in E. coli BL21(DE3)

AKA44603.1, AIE12488.1

expression in Escherichia coli BL21(DE3)

AKA44603.1, AIE12488.1

from E. phaseoloides into pMal-C2X vector; expression in E. coli BL21(DE3)

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

biotechnology

3 entries

biotechnology

the research integrated metabolomics and transcriptomics enabling identification of key enzymes in triterpenoid and flavonoid biosynthesis

biotechnology

AKA44603.1, AIE12488.1

Oleanane-type saponins have the potential to treat many diseases. The research studies showed that oleanane-type ginsenosides with structures similar to that of ginsenoside Ro also had high biological activity

biotechnology

AKA44603.1, AIE12488.1

Oleanane-type saponins have the potential to treat many diseases.The research studies showed that oleanane-type ginsenosides with structures similar to that of ginsenoside Ro also had high biological activity

top print hide References Search

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

Liu, X.; Zhang, L.; Feng, X.; Lv, B.; Li, C.

Biosynthesis of glycyrrhetinic acid-3-O-monoglucose using glycosyltransferase UGT73C11 from Barbarea vulgaris

Ind. Eng. Chem. Res.

14949-14958

2017

Barbarea vulgaris (K4GGT4)

brenda

Erthmann, P.O.; Agerbirk, N.; Bak, S.

A tandem array of UDP-glycosyltransferases from the UGT73C subfamily glycosylate sapogenins, forming a spectrum of mono- and bisdesmosidic saponins

Plant Mol. Biol.

37-55

2018

Barbarea vulgaris

29603041

brenda

Augustin, J.M.; Drok, S.; Shinoda, T.; Sanmiya, K.; Nielsen, J.K.; Khakimov, B.; Olsen, C.E.; Hansen, E.H.; Kuzina, V.; Ekstrom, C.T.; Hauser, T.; Bak, S.

UDP-glycosyltransferases from the UGT73C subfamily in Barbarea vulgaris catalyze sapogenin 3-O-glucosylation in saponin-mediated insect resistance

Plant Physiol.

160

1881-1895

2012

Barbarea vulgaris (K4GKX2), Barbarea vulgaris

23027665

brenda

Xu, X.; Tang, R.; Liu, L.; Zhang, Q.; Xiong, H.; Mei, Z.; Liu, Y.

Comparative transcriptome-metabolome study reveals the functions of pentacyclic triterpene synthase and flavonoid glycosyltransferases in Entada phaseoloides

Int. J. Biol. Macromol.

318

145278

2025

Entada phaseoloides

40523485

brenda